Bacillus fusiformis for promoting rhizosphere growth of blueberries and application thereof

A technology of Bacillus fusiformis and rhizosphere growth promotion, applied in the direction of application, bacteria, biocide, etc., can solve the problems of abnormal growth of microorganisms, achieve the effects of excellent strain resources, increase stem thickness, and promote root growth

Inactive Publication Date: 2013-04-24
SHANDONG FOREST SCI RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the growth-promoting bacteria in the rhizosphere of plants have strong specificity, and the growth conditions of blueberries are relatively special. They are only suitable for growth in acidic soil (4.0-4.8) with high organic matter content, and most of them grow in the environment of pH 4.0-4.8. Microorganisms do not grow normally

Method used

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  • Bacillus fusiformis for promoting rhizosphere growth of blueberries and application thereof
  • Bacillus fusiformis for promoting rhizosphere growth of blueberries and application thereof
  • Bacillus fusiformis for promoting rhizosphere growth of blueberries and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Screening of blueberry rhizosphere growth-promoting Fusilicus bacillus

[0019] (1) Screening process:

[0020] By gradient dilution method, 216 isolates were isolated from blueberry rhizosphere soil samples in Wolin blueberry production base in Jiaonan City, Shandong Province. Purify the isolated bacteria by the three-section line method, and judge whether the strain is purified by microscopic examination, number the purified bacteria, pick a single colony, and transfer it to an LB slant for future use. 14 strains with good green-preserving effect were screened out by the green-preserving method, combined with the radish cotyledon weight-increasing method, a bacterial strain with good growth-promoting effect was finally screened out, which was denoted as L13.

[0021] (2) Colony characteristics and cell morphology:

[0022] After being cultured on LB plates for 24 hours, Bacillus fusiformis L13 forms a milky white colony, which is round, opaque, with neat a...

Embodiment 2

[0029] Example 2 Blueberry rhizosphere growth-promoting Bacillus fusiformis has a growth-promoting effect on blueberry growth

[0030] Activate the preserved blueberry rhizosphere growth-promoting Bacillus fusiformis L13 on the LB slope, pick a ring and insert it into the beef extract peptone medium (beef extract 0.3%, peptone 1%, sodium chloride 0.5%, agar 2%, pH7 .0-7.2, the above are mass percentages), shake culture at 37°C and 180rpm for 2 days. Centrifuge the fermentation broth at 6000rpm for 5min, rinse the bacteria with sterile normal saline for 3 times, adjust the bacterial suspension with sterile normal saline (2.0×10 8 cfu / m) to make inoculants. After the blueberry container seedlings are planted, take 20ml and dilute it 10 times with sterile water and evenly water it around the blueberry roots. Watering the same amount of sterile saline was used as a control, and the root system, seedling height and ground diameter of blueberries were measured three months later, ...

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Abstract

The invention discloses a bacillus fusiformis for promoting rhizosphere growth of blueberries. The bacterial strain is named as bacillus fusiformis (Bacillus fusiformis) L13 in class, and preserved at the general microbiological center of the China Committee for Culture Collection of Microorganisms on December 31, 2012; and the preservation number is CGMCC No.7068. The invention also provides application of the bacillus fusiformis for promoting rhizosphere growth of the blueberries. The bacillus fusiformis for promoting rhizosphere growth of the blueberries disclosed by the invention is screened from rhizosphere soil of the blueberries, adapts with a special rhizosphere soil environment of the blueberries, and can promote growth of the blueberries.

Description

technical field [0001] The invention relates to a blueberry rhizosphere growth-promoting fusiform bacillus and application thereof. Background technique [0002] Blueberry (Vaccinum ssp.), also known as bilberry, lingonberry, blueberry, is a plant of the genus Vaccinium L. in the family Ericaceae. Blueberry fruit is a berry, rich in various vitamins, arbutin, anthocyanin, SOD, tannic acid and flavonoids and other antioxidant compounds. Nutrition and health fruit with extremely high nutritional value, health care function and medicinal value. The International Food and Agriculture Organization lists it as one of the five health foods for human beings, and it is a treasure among fruit trees. [0003] Blueberry is an oligotrophic plant, and excessive or improper fertilization can easily cause damage to the tree. Moreover, most of the fertilizers currently used in blueberry planting are traditional fruit tree fertilizers, which are not only poorly targeted, but also easily ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/07
Inventor 刘方春邢尚军孙磊马海林马丙尧杜振宇杜秉海丁延琴姚良同陈波
Owner SHANDONG FOREST SCI RES INST
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