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Bacillus aryabhattai and application thereof

A technology of Bacillus aristolochia and strains, applied in the field of microbial resources, can solve the problems of few reports of Bacillus, and achieve the effects of improving the photosynthetic ability, high activity and promoting growth of walnuts

Active Publication Date: 2021-02-26
SHANDONG AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, there are many reports about protease-producing bacteria and siderophores produced by bacteria, but there are fewer reports about bacillus that can simultaneously dissociate phosphorus and potassium, produce protease, IAA, siderophores, and significantly promote growth and drought resistance on walnuts

Method used

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  • Bacillus aryabhattai and application thereof
  • Bacillus aryabhattai and application thereof
  • Bacillus aryabhattai and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Strain identification

[0052] In the present invention, a bacterial strain obtained through screening with walnut growth-promoting and drought-resistant effects is determined to be B. aryabhattai JNJ266 through identification of 16S rDNA and physiological and biochemical indicators. Such as Figure 1-2 Shown, the thalline and bacterium colony characteristics of described bacillus arborii (B.aryabhattai) JNJ266 are: JNJ266 is in LB plate culture medium (yeast powder 0.5%, peptone 1%, sodium chloride 1%, distilled water 1000ml, agar 1.6 %, pH 7.0, sterilized at 121° C. for 20 minutes) and cultured for 24 hours, the colonies were regular round, white, without protrusions and folds; the bacteria were rod-shaped, spore-forming, and Gram-positive.

[0053] The physiological and biochemical characteristics of the strain are: negative starch hydrolysis test, positive gelatin liquefaction test, positive contact enzyme test, negative V-P test, negative indole test, ...

Embodiment 2

[0056] Example 2 Experiments on Phosphorus and Potassium Solubilization, Protease, IAA and Siderophore Production of B.aryabhattai JNJ266

[0057] The protease-producing, phosphorus-dissolving, siderophore-producing, IAA-producing and potassium-dissolving abilities of B. aryabhattai JNJ266 were detected, and the results are shown in Tables 1-4.

[0058] Table 1 protease production results

[0059]

[0060]

[0061] Table 2 results of organic phosphorus solution

[0062]

[0063] Table 3 produces IAA measurement result

[0064]

[0065] Table 4 JNJ266 function effect

[0066]

[0067] Note: "+" indicates function

Embodiment 3

[0068] Example 3 Growth-promoting and drought-resistance of B.aryabhattai JNJ266 and its effect on soil

[0069] Preparation of fermentation broth: Transfer the strains preserved on the slant to a test tube containing 5ml of LB liquid medium, and culture them in a constant temperature shaker for 12 hours, temperature: 37°C, speed: 180rpm; 1% of the inoculum was inserted into 50ml LB liquid medium, placed in a shaker for 24 hours, temperature: 37°C, rotation speed: 180rpm, to make a fermentation broth.

[0070] After the walnut seedlings are settled, 10ml of the fermented liquid is taken, diluted to 1000ml, and then treated with root irrigation. Take the treatment without adding bacteria solution as the control; the drought treatment and the control group, while applying the bacteria solution, no longer water after watering until the end of the test (60 days of cultivation after applying the bacteria).

[0071] The dosage when filling the roots is: 10mL per plant in the pot ...

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Abstract

The invention provides a bacillus aryabhattai JNJ266 strain and an application thereof, and belongs to the technical field of microorganisms. The strain is preserved in China General Microbiological Culture Collection Center on August 5, 2020, and the biological preservation number of the strain is CGMCC No. 20483. The strain has the functions of dissolving phosphorus and potassium, producing protease, IAA, siderophore and the like, under the potting condition, root irrigation treatment is carried out on the bacillus asterii fermentation liquor after walnut seedlings are fixed, after the JNJ266 fermentation liquor is applied, the diameter, the plant height, the branch number, the wet weight and the dry weight of the walnut are increased, and the chlorophyll content, the walnut leaf enzymeactivity and the walnut soil enzyme activity are also improved; and under the drought condition, the diameter, the plant height, the branch number, the wet weight and the dry weight of the walnuts treated with JNJ266 fermentation broth increased, and the chlorophyll content, the walnut leaves enzyme activity and the walnut soil enzyme activity are also increased. The bacillus aryabhattai screenedhas good walnut growth-promoting and drought-resisting effects.

Description

technical field [0001] The invention relates to the technical field of microbial resources, in particular to a bacillus arborii and its application. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Walnut (Juglans regia L.) is a Juglans plant with a long history of planting in my country. It is an important economic forest crop in my country. Every part of the walnut tree has important economic value. Walnuts were originally produced in Persia, with a planting history of 7,000 years, and are widely planted around the world. For humans, walnuts are a very long-standing tree food. Although my country is far ahead of other countries in terms of walnut production, the share a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01B79/02C05G3/00C05G5/20C05G5/12C05G5/27C05G5/10C12R1/07
CPCC12N1/20A01B79/02C05C11/00C05G3/00C05G5/20C12R2001/07C12N1/205C05D9/00C05F11/08
Inventor 丁延芹杜秉海汪城墙陈安镜马海林刘凯姚良同刘方春
Owner SHANDONG AGRICULTURAL UNIVERSITY
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