39 results about "Burkholderia lata" patented technology

The present invention relates to compositions for the treatment of conditions, including pancreatic insufficiency. The compositions of the present invention comprise lipase, protease and amylase in a particular ratio that provides beneficial results in patients, such as those afflicted with pancreatic insufficiency. This invention also relates to methods using such compositions for the treatment of pancreatic insufficiency. The compositions specifically comprise crosslinked Burkholderia cepacia lipase crystals, Aspergillus melleus protease crystals and amorphous Aspergillus oryzae amylase in a ratio of about 1:1:0.15 USP units.

The invention belongs to the technical field of microorganisms, and specifically discloses a Burkholderia kururiensis strain and application thereof. The strain is Burkholderia kururiensis yy01, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on March 17, 2015, and has preservation No. CGMCC No. 10631. The strain yy01 has both efficient releasing of phosphorus and potassium and high nitrogenase activity, increases the effectiveness of insoluble phosphorous and potassium in soil and convert free nitrogen in the air into ammonia for absorption and utilization by crops, and has significant growth-promoting effect on rice, tobacco and other crops.

A DNA fragment encoding a β subunit is obtained by inverse PCR using primers designed based on the nucleotide sequence of a N-terminal signal sequence region of a GDH β subunit derived from Burkholderia cepacia KS1 strain.

The invention discloses a burkholderia lata strain PN1 and application thereof. The classification name of the strain is Burkholderia lata, the strain number is PN1, the strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO:M 2019880. The strain PN1 screened out from phyllostachys edulis root systems has a significant inhibiting effect on phyllostachys edulis shoot blight pathogens ceratosphaeria phyllostachydis, a bacteriostasis rate of fermentation broth of the strain PN1 screened out from the phyllostachys edulis root systems can reach58.82%, and the strain PN1 screened out from the phyllostachys edulis root systems has efficient capability of phosphate solubilizing and IAA secretion; and the phyllostachys edulis endophytic bacteria PN1 can significantly promote growth of phyllostachys edulis seedlings, and test data shows that a liquid bacterial agent produced from the strain PN1 significantly increases growth indexes of the ground diameter, the seedling height, chlorophyll and the like of phyllostachys edulis. Therefore, excellent strain resources are provided for development of an environment-friendly bio-fertilizer special for the phyllostachys edulis.

The invention discloses a genetic liquid phase chip for rapid detection of five drastic pathogenic bacteria of bacillus anthracis, yersinia pestis, brucella bacteria, francisella tularensis and burkholderia pseudomallei. The liquid phase chip for detecting the five drastic pathogenic bacteria has the advantages of large flux, less required sample capacity, strong specificity, high sensitivity, accuracy, high efficiency, and the like.

A respirable composition for treatment of a bacterial infection includes one or more active bacteriophages in combination with a pharmaceutically acceptable respirable carrier. The composition includes a carbohydrate carrier, and is prepared as fine powder. In another aspect, bacteriophages are provided in a liquid carrier for administration by nebulization. In one aspect, the bacteriophages have anti-bacterial activity against one or more species or strains of Burkholderia cepacia complex (BCC) bacteria. The invention further relates to the use of a BCC bacteriophage to treat a BCC infection, in particular in an individual suffering from cystic fibrosis.

Provided are antimicrobial agents produced from Burkholderia cepacia complex (Bcc) bacteria, in particular from bacteria which comprise a cluster of polyketide synthesis genes. Also provided is use of the antimicrobial agents in the treatment of disease. Further provided are methods for producing antimicrobials, methods for detecting antimicrobial producing bacterial strains and kits for use in the methods.

Disclosed is a method for producing (1S,2R)-2-chloro-2-fluorocyclopropanecarboxylic acid with a high yield and high selectivity. Specifically disclosed is a method for producing (1S,2R)-2-chloro-2-fluorocyclopropanecarboxylic acid, wherein (1S)-2-chloro-2-fluorocyclopropanecarboxylic acid ester is hydrolyzed using an esterase derived from Burkholderia cepacia.

The invention discloses a molecular beacon and a kit for quickly identifying various clinically common bacteria and belongs to the technical field of microbiological detection. The sequences SEQ ID of the molecular beacon are as shown in v6p1, v6p2, v1p1 and v1r. Through comparison between various clinically common bacteria 16s ribosome RNA sequences in an NCBI gene sequence database and design of the molecular beacon at a tag sequence, clinically common 18 bacteria, including baumanii, A.hydrophila, burkholderia cepacia, citrobacter freundii, enterobacter cloacae, enterococcus faecium, enterococcus faecalis, enterobacter aerogenes, escherichia coli, klebsiella pneumoniae, listeria monocytogenes, proteus mirabilis, pseudomonas aeruginosa, staphylococcus epidermidis, staphylococcus aureus, salmonella, serratia marcescens and stenotrophomonas maltophilia, can be quickly identified. The invention further discloses the kit containing the molecular beacon and used for detecting various bacteria, and the kit can quickly and accurately identify various clinically common bacteria.

The invention belongs to the technical field of kitchen waste treatment, and specifically discloses a method for improving the yield of biodiesel produced by enzymatically preparing kitchen grease by utilizing pretreatment. The method comprises the following steps: S1. removing sundries in the kitchen waste, and sequentially subjecting the kitchen waste to microwave, ultrasonic and hydrothermal treatment; S2. separating the three phases to obtain the upper layer of grease; S3. passing in ozone for oxidation; S4 .Add immobilized compound lipase to the oil obtained in S3, and add short-chain alcohol to carry out transesterification to prepare biodiesel; wherein, the immobilized compound lipase described in S4 includes Geotrichum candidum lipase, Candida lipase and onion lipase Burkholderia lipase. The invention adopts microwave, ultrasonic and hydrothermal treatment to jointly treat kitchen waste, and the amount of kitchen grease precipitation increases by 10-50% compared with that before treatment, and increases by 2-30% compared with single pretreatment, effectively increasing the amount of kitchen grease precipitation, and significantly Improve the yield and yield of biodiesel produced by enzymatic method.

The invention discloses microbial population sensing signal quenching bacteria and application thereof as biocontrol bacteria. Through study, the invention finds that Burkholderia cepacia has an obvious degradation effect on a population sensing signal molecule DSF; meanwhile, the Burkholderia cepacia F25 for efficiently degrading the DSF signal molecule is obtained by screening. The strain was preserved in the Guangdong Province Microbiological Culture Center on April 3, 2018, with a preservation number of GDMCC No:60346. The bacteria can quickly and efficiently degrade the DSF, and has population sensing and quenching functions and remarkable biocontrol effects on DSF pathogenic diseases such as black rot caused by xanthomonas campestris XC1. The microbial population sensing signal quenching bacteria disclosed by the invention can replace a chemical control method and eliminate potential safety hazards of antibiotic use, is environmentally-friendly and has important application valuefor preventing and treating plant diseases caused by pathogens relying on the DSF.

This invention relates to methods and compositions for increasing immunity against, and survival of, a bacterial infection by inhibiting Target of Rapamycin (TOR) complex 2 or TORC 2. In particular, the current invention is useful in increasing immunity and survival after infection by Burkholderia cepacia as well as other bacteria since the agents that target TORC2 increase host tolerance of infection rather than target the clearance or containment of specific types of bacteria. This invention also relates to methods and compositions for increasing immunity against, survival of, and host tolerance to a bacterial infection by inhibiting the circadian regulator, Period protein.

The invention discloses a method for detecting Burkholderia pseudomallei by multi-cross isothermal amplification in combination with gold nano-biosensing. Corresponding specific primers is designed based on Burkholderia pseudomallei TTS1 genes, and meanwhile fluorescein is marked at the 5'end of an amplification primer C1 and biotin is marked at the 5'end of an amplification primer D2 to obtain C1* and D1 * newly labeled amplification primers. The method can realize visual detection of the Burkholderia pseudomallei via the gold nano-biosensor, and the method is convenient, rapid, high in sensitivity and good in specificity.

The invention discloses a special compound microorganism bacterium agent capable of eliminating continuous cropping obstacles of radix pseudostellariae. The special compound microorganism bacterium agent is prepared from common probiotics and aboriginal probiotics at the volume ratio of 4 to 6, wherein the common probiotics are prepared by mixing lactic acid bacteria, photosynthetic bacteria, saccharomycetes and actinomyces at the volume ratio of 1 to 1 to 1 to 1; the aboriginal probiotics are prepared by mixing burkholderia, pseudomonas and bacillus at the volume ratio of 4 to 1 to 1. The invention further discloses a preparation method of the special compound microorganism bacterium agent capable of eliminating the continuous cropping obstacles of the radix pseudostellariae. The preparation method comprises the following steps: step 1, screening aboriginal antagonistic bacteria; step 2, preparing common compound bacterium liquid; step 3, activating and carrying out expanding culture on the aboriginal probiotics; step 4, preparing the compound microorganism bacterium agent. The special compound microorganism bacterium agent prepared by the method has a good root fixed planting capability and is safe and reliable, has obvious prevention and control effects on the continuous cropping obstacles of the radix pseudostellariae and soil-borne diseases, and has no pathogenic infection capacity on other succession crops.

The invention discloses a strain of Burkholderia cepacia and its application, belonging to the field of microorganisms. The Burkholderia cepacia ZWS15 was deposited in the China Center for Type Culture Collection on November 6, 2017, with the preservation number CCTCC NO: M2017661, and the preservation address is Wuhan University, Wuhan, China. The bacterium can use cholesterol as the sole carbon source, can grow at 50°C, tolerates 10% organic solvents, and the enzyme activity of cholesterol oxidase in the crude enzyme liquid obtained by its fermentation is as high as 200U / L. Using Burkholderia cepacia ZWS15 fermented crude enzyme solution to catalyze cholesterol in a two-phase system in the presence of organic solvents, the amount of cholest-4-en-3-one collected was about 5-11% of the added amount of substrate %, the amount of cholest-4-ene-3,6-dione is about 10-15% of the amount of substrate added.