A special compound microbial agent for eliminating the obstacle of Pseudostellaria heterophylla and its preparation method

A technology of compound microbial agent and continuous cropping obstacle, which is applied in the field of special compound microbial agent and its preparation to eliminate the obstacle of continuous cropping of Radix Pseudostellariae. It can solve the problems of inability to colonize beneficial bacteria and deterioration of soil environment, and achieve rhizosphere colonization ability Good, activate soil nutrients, the effect is obvious

Active Publication Date: 2020-12-01
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The continuous cropping of Pseudostellariae Pseudostellariae significantly deteriorated the soil environment, and Pseudostellariae Pseudostellariae continued to release a variety of secondary metabolites and medicinal components, which may prevent the beneficial bacteria in the commercially available microbial agents from colonizing in the rhizosphere of Pseudostellariae Pseudostellariae. The host specificity of soil-borne pathogens and the particularity of continuous cropping soil environment should be considered comprehensively when using microbial agents to control the replanting diseases of heterophylla heterophylla

Method used

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  • A special compound microbial agent for eliminating the obstacle of Pseudostellaria heterophylla and its preparation method
  • A special compound microbial agent for eliminating the obstacle of Pseudostellaria heterophylla and its preparation method
  • A special compound microbial agent for eliminating the obstacle of Pseudostellaria heterophylla and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Screening and identification of indigenous antagonistic bacteria

[0042] Pseudomonas selective medium and Bacillus selective medium were used to isolate Pseudomonas and Bacillus from the rhizosphere of Radix Pseudostellariae, and inorganic medium (containing higher concentrations of autotoxic substances in Radix Pseudostellariae) was used to isolate Toxin-degrading bacteria were isolated.

[0043] Randomly selected single colonies on the above-mentioned different media were used to face off on PDA plates to evaluate their antagonistic effects on heterophyllosis-specific pathogenic bacteria (such as Fusarium oxysporum, Fusarium moniliforme, and ankle mold, etc.). Bacteria with strong antagonistic ability were identified by 16s rRNA gene or 16s-23s rRNA interval PCR identification for molecular identification. 16s rRNA gene PCR amplification upstream and downstream primers are: 27f (5'-AGAGTTTGATCCTGGCTCAG-3') and 1522r (5'-AAGGAGGTGATCCAGCCGCA-3'); 16s-23s rR...

Embodiment 2

[0050] The preparation of embodiment 2 special-purpose composite microbial bacterial agents

[0051] A kind of special-purpose composite microbial bacterial agent described in the present invention is formed by mixing general-purpose beneficial bacteria and native beneficial bacteria in a ratio of 4:6 by volume, and the specific preparation method is as follows:

[0052] 2.1 Preparation of EM composite bacterial solution

[0053] Add 300g of brown sugar and 50mL of MS mother liquor to 18L of well water (tap water needs to be left for at least 2 days before use), then add 1L of activated EM liquid bacteria, and place it in a room at 35-37°C for 2-3 weeks of closed fermentation. From the second day of fermentation, you can open the mouth of the airtight container to deflate and depressurize once every other day. When you open the mouth of the container, you can smell a sweet and sour smell, which becomes more and more obvious. This is a normal phenomenon, which means that the fe...

Embodiment 3

[0063] Example 3 Evaluation of Field Effect of Special Compound Microbial Inoculant to Prevent and Control Pseudostellaria Pseudostellariae Continuous Cropping Obstacles

[0064] First, the prepared special compound microbial agent is fermented together with organic materials to prepare bio-organic fertilizer, which is used as base fertilizer to pre-improve the soil of Pseudostellariae heterophylla, and then the plots are prepared to plant Pseudostellaria heterophylla, and proper amount shall be applied from the seedling stage of Pseudostellaria heterophylla Composite microbial liquid inoculum specially used for root irrigation and topdressing, and the yield difference was compared during the harvest period. The specific preventive measures are as follows:

[0065] 3.1 Preparation of microbial organic base fertilizer

[0066] Mix the organic materials bean dregs, rice bran, dried pig manure, fish meal, and brown sugar in a weight ratio of 2:1:1:1:0.03, then add an appropriate...

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Abstract

The invention discloses a special compound microorganism bacterium agent capable of eliminating continuous cropping obstacles of radix pseudostellariae. The special compound microorganism bacterium agent is prepared from common probiotics and aboriginal probiotics at the volume ratio of 4 to 6, wherein the common probiotics are prepared by mixing lactic acid bacteria, photosynthetic bacteria, saccharomycetes and actinomyces at the volume ratio of 1 to 1 to 1 to 1; the aboriginal probiotics are prepared by mixing burkholderia, pseudomonas and bacillus at the volume ratio of 4 to 1 to 1. The invention further discloses a preparation method of the special compound microorganism bacterium agent capable of eliminating the continuous cropping obstacles of the radix pseudostellariae. The preparation method comprises the following steps: step 1, screening aboriginal antagonistic bacteria; step 2, preparing common compound bacterium liquid; step 3, activating and carrying out expanding culture on the aboriginal probiotics; step 4, preparing the compound microorganism bacterium agent. The special compound microorganism bacterium agent prepared by the method has a good root fixed planting capability and is safe and reliable, has obvious prevention and control effects on the continuous cropping obstacles of the radix pseudostellariae and soil-borne diseases, and has no pathogenic infection capacity on other succession crops.

Description

technical field [0001] The invention relates to the technical field of microbes and biological control, in particular to a special compound microbial agent for eliminating the obstacle of joint operation of Pseudostellariae and a preparation method thereof. Background technique [0002] Continuous cropping obstacle refers to the phenomenon that even under normal field management measures, the same plant is planted in the same field for many years, resulting in poor growth and development of plants, serious pests and diseases, and a decline in yield and quality. It is also called allelopathic autotoxicity or replanting disease. Continuous cropping of medicinal plants is generally prohibited. According to statistics, about 70% of root medicinal materials have different degrees of continuous cropping obstacles, which seriously hinders the sustainable development of my country's modern pharmaceutical industry. [0003] Years of field observations have found that the continuous c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/16C12N1/20A01N63/32A01N63/20A01N63/27A01N63/22A01N63/28A01P21/00A01P1/00A01P3/00C12R1/25C12R1/46C12R1/245
CPCA01N63/00A01N63/30C12N1/14C12N1/16C12N1/20A01N2300/00
Inventor 吴林坤林文雄陈军吴红淼林生
Owner FUJIAN AGRI & FORESTRY UNIV
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