A kind of functional composite microorganism seedling-raising matrix and its preparation method and application

A technology of composite microorganisms and seedling-raising substrates, which is applied in the field of functional composite microbial seedling-raising substrates, can solve problems such as unbalanced nutrient supply, unstable field effects, and poor colonization ability of biological bacteria agents, and achieve good rhizosphere colonization capabilities, High soluble phosphorus content, increase the effect of emergence rate

Active Publication Date: 2022-04-22
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are some biological agents used in agricultural production on the market, but due to the poor colonization ability of biological agents in plant roots, the supply of nutrients is not balanced, and the field effect is usually unstable

Method used

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  • A kind of functional composite microorganism seedling-raising matrix and its preparation method and application
  • A kind of functional composite microorganism seedling-raising matrix and its preparation method and application
  • A kind of functional composite microorganism seedling-raising matrix and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Isolation and screening of strain NJHR92:

[0048] Soil samples were collected from the plastic greenhouses in the tomato planting area of ​​Houcun, Qilin Town, Nanjing. They were collected from the rhizosphere soil of healthy plants in the field of bacterial wilt disease. The healthy plants were uprooted and shaken gently. After most of the soil was shaken, the rest were close to the roots. The soil together with part of the fibrous roots was used as the rhizosphere soil. Take 1g of the rhizosphere soil and place it in a 50mL Erlenmeyer flask (containing glass beads) filled with 9mL of sterile water, vibrate at 170rpm for 30min, and after serially diluting the soil suspension 10 times, take 10 8 The suspension was spread on the NA medium plate and cultured at 30°C for about 48h. A single colony was picked from the NA medium plate, streaked on the NA medium plate for purification, and strain NJHR92 was obtained.

[0049] NJHR92 belongs to Lysinibacillus sphaericus, sph...

Embodiment 2

[0057] 1. Strain culture method

[0058] Strains NJHR92 and NJQL-A6 were streaked with NA solid medium, and activated in a 30°C incubator for 48 hours; single colonies of NJHR92 and NJQL-A6 were respectively picked and placed in NA liquid medium, 30°C, 170r min -1 Cultivate overnight on a shaking table to obtain NJHR92 fermentation broth and NJQL-A6 fermentation broth respectively; resuspend the bacteria in normal saline, 4500r min -1 Centrifuge for 5 minutes to collect the bacteria, repeat 3 times to wash to remove the residual medium, and finally adjust the OD with sterile saline 600 =0.5 NJHR92 seed solution, OD 600 =0.5 NJQL-A6 seed solution.

[0059] Add NJHR92 seed solution, NJQL-A6 seed solution and functional bacteria combination seed solution (NJHR92 seed solution and NJQL-A6 seed solution according to the volume ratio of 1:1) to 96-well cells containing NA liquid medium at an inoculation amount of 1%. Culture plate (Costar), 170r min -1 , Cultivate on a shaker at...

Embodiment 3

[0071] The colonization quantity investigation of embodiment 3 bacterial strains in tomato rhizosphere

[0072] For the root colonization experiment, the substrate with a volume ratio of distiller's grains:cow dung compost:vermiculite 62:15:20 was added to the tissue culture bottle, the water content of the substrate was adjusted to 40% with tap water, and sterilized at 121°C for 1 hour. Cool to room temperature, and then operate the test in a super-clean bench, plant 4 dew-white tomato seedlings in each tissue culture bottle that have been germinated at 25-28°C, repeat 3 times, and insert the seed solution of NJHR92+NJQL-A6 functional bacteria combination (same as Example 2, NJHR92 and NJQL-A6 were cultured overnight in NA liquid medium respectively, and the bacteria were collected by centrifugation, and the bacteria suspension was made with sterile physiological saline, and the OD was adjusted 600 =0.5, NJHR92 seed solution and NJQL-A6 seed solution were obtained respectivel...

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Abstract

The invention discloses a functional composite microbial seedling-raising substrate, which is prepared by inoculating microorganisms NJHR92 and NJQL‑A6 in a seedling-raising substrate composed of wine tanks, cow dung compost and vermiculite as raw materials and then fermenting into; bacterial strain NJHR92, classified as Bacillus sphaericus, and the strain preservation number is CGMCC No.6629; bacterial strain NJQL‑A6, classified and named as Ralstonia piterii, and the strain preservation number is CGMCC No. 6628. The invention also discloses the application of the functional compound microorganism seedling-raising matrix in cultivating the seedlings of melon, fruit and vegetable crops. The functional compound microbial seedling-raising substrate of the present invention not only provides growth medium and nutrients for plants, promotes plant growth, but also has the function of preventing and controlling diseases, increases the emergence rate of seeds, cultivates high-quality seedlings, enhances the adaptability of seedlings, and enhances their Stability of growth after transplanting to field.

Description

technical field [0001] The invention belongs to the technical production field of agricultural microorganisms, and relates to a functional composite microorganism seedling-raising substrate, in particular to a functional composite microorganism seedling-raising substrate added with bacterial strains NJHR92 and NJQL-A6. Background technique [0002] With the improvement of living environment quality requirements, the reduction of arable land area, and the intensive development of agricultural production, the seedling substrate has the advantages of energy saving, high production efficiency, good seedling quality, and high survival rate of slow seedlings. It is widely used in the cultivation of flowers and so on. High-quality seedlings are the prerequisite for high-yield and high-quality crops, and the substrate is the basis for ensuring high-quality seedlings. The use of substrate seedling technology is the direction of promotion in the future. The function of the plant seed...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/04C05F17/20C05G3/00A01G24/20A01G24/15A01G24/22A01G31/00C12R1/01
CPCA01G24/15A01G24/20A01G24/22A01G31/00C12N1/20C12P1/04C05C11/00C05F17/00C05G3/00C12R2001/01C12N1/205C05D9/00C05F3/00C05F5/008C05F11/08Y02W30/40
Inventor 徐阳春韦中杨清俊浩折霞沈其荣
Owner NANJING AGRICULTURAL UNIVERSITY
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