26 results about "Lysinibacillus sp." patented technology

The invention discloses a strain of Lysinibacillus bacilli B1 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653, and the preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653. The bacstein lysine bacillus has an inhibition effect on penicillium expansum, aspergillus ochraceus, botrytis cinerea, escherichia coli, listeria monocytogenes, sclerotium rolfsii, root rot bacteria, ralstonia solanacearum and fusarium oxysporum. The strain can be used for preventing and treating southern blight and root rot of siraitia grosvenorii, banana fusarium wilt, mycosis of navel oranges and the like; the bacterial fertilizer can be applied to the fields of fruit preservation and bacterial fertilizer biocontrol of the plants; meanwhile, the strain can also be applied to the field of food preservation, is a bacterial strain with multiple functions and wide application fields, and has a good application prospect.

The invention belongs to the field of resource utilization of livestock and poultry manure, and particularly relates to a thermophilic ammoniation compound microbial agent, a fertilizer and application. The thermophilic ammoniation compound microbial agent is prepared by compounding lysinibacillus spp., bacillus megatherium and white-rot fungi, the preservation number of the lysinibacillus sphaeroides is CCTCC NO: M 20242776, the preservation number of the bacillus megaterium is CCTCC NO: M 20242777, the preservation number of the white-rot fungi is CCTCC NO: M 20242778, and the three strains are all preserved in the China Center for Type Culture Collection. Tests prove that the thermophilic ammoniation compound microbial agent shows higher ammoniation activity at 60 DEG C, and can be used for promoting the compost recycling process and improving the compost product quality.

The application discloses a kind of synthetic functional microbial inoculants and its application in salt-tolerant growth promotion of wheat, adopt hydrophobic gordon's bacteria ( Gordonia hydrophobica ), odyssey lysine bacillus ( Lysinibacillus odysseyi ), sea bed motility microorganism ( Planomicrobium okeanokoites ), north China broussonetia yellow color bacterium ( Luteimonas huabeiensis ), epidermis brevibacterium ( Brevibacterium epidermidis ) five strains of bacteria, microbial inoculant has no antagonism between strains, salt-tolerant, and other significant characteristics such as viable count, can significantly promote spring wheat germination rate, seedling length and root length, and has significant promoting effect on seedling length and root length of winter wheat.Under salt stress conditions, the microbial inoculant can significantly improve the activities of catalase, peroxidase and superoxide dismutase of wheat, and the total chlorophyll content;after treatment with the microbial inoculant, the contents of malondialdehyde and proline of wheat are significantly reduced.The microbial inoculant plays a promoting and protecting role in the process of resisting salt stress of spring wheat and winter wheat, and has an impact on the development and sustainable development of arid region agriculture.

The invention belongs to the field of resource utilization of livestock and poultry manure, and particularly relates to a spindle-shaped lysine bacillus, fermentation liquor, an ammonifying bacterium agent and application. The lysinibacillus fusiforme is preserved in the China Center for Type Culture Collection, and the preservation number of the lysinibacillus fusiforme is CCTCC (China Center for Type Culture Collection) NO: M 20242776. Tests prove that the ammoniating bacterium inoculant prepared by utilizing the lysine bacillus spunlaginosus shows higher ammoniating activity at 37 DEG C, and the ammoniating bacterium inoculant can promote the composting recycling process and improve the composting product quality by inoculating the ammoniating bacterium inoculant.

The invention provides lysinibacillus sphaericus WSF-65, a fumigation preparation and application of the lysinibacillus sphaericus WSF-65 and belongs to the technical field of biological prevention and control, the Latin name of the bacillus sphaericus is Lysinibacillus sphaericus, the strain number of the bacillus sphaericus is WSF-65, and the preservation number of the bacillus sphaericus is CCTCC (China Center for Type Culture Collection) NO.M20251104. The Lysinibacillus sphaericus WSF-65 disclosed by the invention can inhibit the occurrence of the citrus anthracnose through generated volatile substances, so that the Lysinibacillus sphaericus WSF-65 can be developed into a fumigation preparation for controlling the occurrence of the citrus anthracnose; the lysinibacillus sphaericus WSF-65 has a certain broad-spectrum inhibition effect on the pathogenic fungi after the citrus is picked, and fermentation liquor of the lysinibacillus sphaericus WSF-65 can be adopted to soak the picked citrus fruits, so that the rotting of the citrus fruits caused by the pathogenic fungi during the storage and transportation period after the citrus is picked is reduced; the inhibition effect of the biocontrol bacterium WSF-65 on pathogenic fungi is similar to that of a chemical bactericide commonly used in the market.

The invention relates to the technical field of microorganisms, in particular to lysinibacillus elongate E and application of the lysinibacillus elongate E in prevention and treatment of root-knot nematodes. The invention provides a microbe, namely, lysinibacillus elongate E, capable of effectively preventing and treating root-knot nematodes. The preservation number of the microbe is CGMCC (China General Microbiological Culture Collection Center) No.33877. The effect of the strain is verified through indoor nematode killing activity determination and field tests, and results show that in an indoor nematode killing activity determination experiment, the fatality rate of fermentation liquor of the long lysine bacillus E strain on nematodes is close to 100%, the effect is the best, 50% fermentation liquor still keeps very high activity (87.56%), and the fermentation liquor still keeps very high activity (87.56%). After the supernate of the fermentation liquor of the long lysine bacillus E strain is sterilized, the effect is slightly reduced from 92.38% to 88.42%; in a field test, the strain can also effectively prevent and treat tomato root knot nematode disease, and the prevention and treatment effect is 48.56%.

This invention discloses a lysinibacillus macroides bacterium that degrades Alternaria alternata toxins and its applications, belonging to the field of microbial technology. The lysinibacillus macroides is deposited at the China Center for Type Culture Collection (CCTCC), Wuhan University, Hubei Province, with accession number CCTCC No. M20241816. This invention is the first to obtain a lysinibacillus macroides bacterium that can effectively reduce and degrade Alternaria alternata toxins, which helps improve the quality of agricultural products (including aquatic products).

The application provides a lysinibacillus sphaericus for producing lipase and application thereof, and relates to the technical field of microbial enzymes. Lysinibacillus sphaericus Yin-202501, and a preservation number of CCTCC No.M2025574.The lysinibacillus sphaericus for producing lipase provided by the application has the following characteristics: Lysinibacillus sphaericus Yin-202501 has the following characteristics: high lipase activity, simple process, and the like. Lysinibacillus sphaericus Yin-202501 is used as an enzyme-producing strain, and olive oil emulsifier and ferrous sulfate are appropriately added in Luria.Bertani (LB) liquid medium, so that the method for preparing a high-activity lipase crude solution by using lysinibacillus sphaericus has the characteristics of simple operation, simple process, good repeatability and the like.

The invention belongs to the technical field of microorganisms, and particularly relates to an IAA-producing strain XY11 and application of the IAA-producing strain XY11 in inhibition of activity of heavy metal cadmium. The invention discloses a strain XY11, the taxonomic name of which is Lysinibacillus sp. The strain XY11 is preserved in Guangdong Microbial Culture Collection Center on July 1, 2025, and the preservation number of the strain XY11 is GDMCC No: 66620. According to the present invention, the morphological observation and the molecular biological identification are performed on the strain XY11, such that the strain XY11 is determined to be Lysinibacillus sp. In addition, tests find that the strain XY11 has the effects of selenium resistance, cadmium resistance, IAA production, selenium activation and cadmium reduction, can improve the content of effective selenium in soil and reduce the content of effective cadmium in soil, and is beneficial to selenium enrichment and cadmium reduction of crops; meanwhile, the growth of crops can be promoted, the selenium and cadmium stress resistance of the crops is improved, and the method has important significance for producing selenium-rich green products.

The application discloses a microbial substrate for strawberry planting and a preparation method and application thereof, and the substrate is made of peat, perlite, bacteria-loaded biochar and fungi, and the volume ratio of the peat, the perlite and the bacteria-loaded biochar is (45-75):25:(10-30); the bacteria-loaded biochar is prepared by loading biochar with a biocontrol composite bacteria; and the biocontrol composite bacteria is composed of Rhodopseudomonas palustris, Bacillus subtilis and Bacillus licheniformis. The application changes the traditional single bacterial group into a mixed bacterial group, and uses biochar as a microbial loading material, so that the mixed bacterial group can better improve the prevention and treatment effect, and the biochar can provide a carbon source for the microorganisms and promote the better reproduction and growth of the microorganisms.

The invention discloses a functional composite microbial inoculant for ecological improvement of moderate and severe saline-alkali soil and a preparation method and application thereof. The composite bacterial agent is prepared by compounding bacterial solutions of paenibacillus polymyxa SMr02, bacillus velezensis DS-R-B-3-5, bacillus laterosporus BHr6-5, lysinibacillus fusiformis SCB19 and pseudomonas japonicus D109 according to the volume ratio of 3: 2: 2: 2: 1. The complex microbial inoculant disclosed by the invention has good saline-alkali tolerance, can obviously improve the stable soil fertility and nutrient content in moderate and severe saline-alkali soil, reduce the soil salinity and alkalinity, relieve saline-alkali stress of salt-tolerant crops and promote growth of the salt-tolerant crops, is simple and convenient to operate, low in cost and environment-friendly, and has a wide application prospect. The method is suitable for ecological improvement of moderate and severe saline-alkali soil in arid areas, irrigation areas in the middle and upstream of the Yellow River and the like, and has good ecological benefits and economic benefits.

The invention discloses a compound flora fermentation method and application of polygonatum cyrtonema, and relates to the technical field of microbial ferment.The method comprises the steps that polygonatum cyrtonema is used as a raw material and subjected to liquid nitrogen quick-freezing and freeze-drying, grinding and screening with a 60-mesh sieve for pretreatment; according to the method, the total sugar concentration of the polygonatum sibiricum can reach 28.75 mg / mL, the polysaccharide concentration of the polygonatum sibiricum can reach 27.70 mg / mL, the yield of the polygonatum sibiricum can reach 34.63%, and the total sugar concentration, the polysaccharide concentration and the yield of the polygonatum sibiricum are obviously higher than those of single-bacterium fermentation, double-bacterium fermentation and three-bacterium fermentation; the increase of the flavone concentration of a fermentation product is superior to that of a blank group, the DPPH free radical scavenging rate reaches 73%, and the pH is reduced to 3.0 at the final stage of fermentation. The extraction rate and biological activity of effective components are improved through the synergistic effect of florae, the process is stable and controllable, and the method is suitable for the fields of food, health care products and medicines and has good application prospects.

The invention provides lysinibacillus for producing lipase and application of the lysinibacillus, and relates to the technical field of microbial enzymology. The lysinibacillus subtilis for producing the lipase is named as Lysinibacillus capsici Yin-202501, and the preservation number of the lysinibacillus capsici Yin-202501 is CCTCC (China Center for Type Culture Collection) No. M2025574. The Lysinibacillus capsici Yin-202501 disclosed by the invention has the following characteristics that the activity of producing the lipase is high, and the process is simple. According to the method, Lysinibacillus capsici Yin-202501 is used as an enzyme producing strain, a proper amount of olive oil emulsifier and ferrous sulfate are added into a Luria. Bertani (LB) liquid culture medium, the method for preparing the high-activity lipase crude solution from the lysinibacillus has the advantages of being easy to operate, simple in process, good in repeatability and the like, the prepared lipase solution is high in activity, the yield is high, and the method is suitable for industrial production. The method has good application potential and development and utilization prospects in the industrial field.

The application discloses an ethyl carbamate degrading strain and application thereof, and belongs to the field of food biotechnology. The application provides fusiform lysinibacillus ZYXW-J9-5 which can efficiently degrade ethyl carbamate, and the preservation number is GDMCC No. 64938. The fusiform lysinibacillus ZYXW-J9-5 has the advantages of fast growth speed, the ability of degrading ethyl carbamate, high production of ethyl carbamate hydrolytic enzyme, excellent fermentation performance and probiotic performance, and is a safe strain and can be applied in the field of food. The strain can be used as a fermenting agent to degrade EC in fermented food, has obvious effect of degrading EC, is convenient to use, has low production cost, and can be widely applied in fermented food such as wine beverage, fermented dairy product, soy sauce, vinegar and the like.

The invention discloses a coupled nitrogen catabolism compound microbial agent and application thereof, and belongs to the technical field of biology. According to the method, an efficient ammonification strain (lysinibacillus L11) and a direct ammoxidation strain (alcaligenes M8) are screened, an ammonification-Dirammox coupling system is constructed, the strain co-culture time sequence (L11 is inoculated 48 hours earlier) is optimized, and organic nitrogen is directly converted into N2. According to the method, the total nitrogen removal rate in the synchronous ammoniation and nitrification culture medium reaches 96.84%, and the ammonia nitrogen removal rate reaches 99.37%. In practical application, the ammonia nitrogen in effluent of a sewage treatment system is stabilized at 9.44 mg / L and COD (Chemical Oxygen Demand) is reduced to 455.98 mg / L by adding the microbial agent, which is obviously superior to that of the traditional process. The method provided by the invention solves the problems of low treatment efficiency of high-ammonia-nitrogen wastewater and serious secondary pollution, and is suitable for co-treatment of aging leachate and perishable landfill leachate.

The invention discloses lysinibacillus, lysinibacillus high-density fermentation liquor and a preservation method of the lysinibacillus high-density fermentation liquor. The lysine bacillus disclosed by the invention can be used for efficiently synthesizing auxin indoleacetic acid (IAA) and has good saline-alkaline resistance, and tests show that the strain and fermentation liquor thereof have a remarkable promoting effect on plant growth; by optimizing the fermentation process of the lysinibacillus, the thallus density and the IAA yield can be remarkably improved, the high-density fermentation liquor of the lysinibacillus can be obtained, the unification of high-density fermentation and high-activity metabolites is realized, the process is stable, and the repeatability is good. The lysinibacillus high-density fermentation liquor is further subjected to special treatment and is matched with a specific composite protective agent, so that the vitality of thalli can be effectively maintained, the normal-temperature storage life of the fermentation liquor is remarkably prolonged, and the problems of short shelf life and poor storage and transportation stability of a liquid microbial inoculum are solved.

The invention belongs to the technical field of microorganisms, and particularly relates to a selenium-tolerant cadmium-tolerant strain XY10 and application thereof in IAA production. The invention relates to a strain XY10, the taxonomic name of which is Lysinibacillus macrorrhizus, and the strain XY10 is preserved in Guangdong Microbial Culture Collection Center on July 1, 2025, and the preservation number is GDMCC No: 66619. According to the invention, the strain XY04 is subjected to morphological observation and molecular biological identification, and it is determined that the strain XY10 is lysinibacillus macroides, and the strain XY04 and the strain XY10 are lysinibacillus macroides. In addition, tests find that the strain XY10 has the effects of selenium resistance, cadmium resistance, IAA production, selenium activation and cadmium reduction, can improve the content of effective selenium in soil and reduce the content of effective cadmium in soil, and is beneficial to selenium enrichment and cadmium reduction of crops; meanwhile, the growth of crops can be promoted, the selenium and cadmium stress resistance of the crops is improved, and the method has important significance for producing selenium-rich green products.

The invention relates to a kit for rapidly screening alkali-producing microorganisms, lysinibacillus pekinensis and application of the lysinibacillus pekinensis, and belongs to the technical field of microorganisms. The invention provides a kit for rapidly screening alkali-producing microorganisms. The kit comprises an acid-base indicator and a 96-hole cell culture plate. The acid-base indicator and the 96-hole cell culture plate are used as the kit for rapidly screening the alkali-producing microorganisms, so that the alkali-producing microorganisms can be rapidly and efficiently screened. The invention also provides the Lysinibacillus pinottii GDAAS 10355 which is preserved in the Guangdong Microbial Culture Collection Center, the preservation number is GDMCC No: 66348, and the preservation time is May 16, 2025. The invention also provides the Lysinibacillus pinottii GDAAS 10355, and the Lysinibacillus pinottii GDAAS 10355 is preserved in the Guangdong Microbial Culture Collection Center, the preservation number is GDMCC No: 66348. Experiments prove that the lysinibacillus pekinensis has the effect of increasing the pH value of the acid soil and can be applied to preparation of a preparation for improving the acid soil.

The invention belongs to the technical field of microorganisms, and particularly relates to lysine bacillus with efficient degradation capacity on carbaryl, the lysine bacillus is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is M20251846. The invention further provides a fungicide prepared from the strain and application of the fungicide. The strain or the fungicide thereof provided by the invention can be applied to the fields of agricultural soil remediation, water pollution treatment and pesticide residue degradation, and can promote degradation of carbaryl or a degradation intermediate 1-naphthol thereof in the environment.

The vinasse bacterial fertilizer is prepared by mixing a bacterial suspension and vinasse according to a mass ratio, the bacterial suspension comprises a bacterial suspension of bacillus amyloliquefaciens JPB35 and a bacterial suspension of lysine bacillus JPB31, and the OD600 value of the bacterial suspension is 0.5 to 0.6. Bacillus amyloliquefaciens JPB35 and lysine bacillus JPB31 separated from vinasse are mixed with the vinasse to prepare the microbial fertilizer, the microbial fertilizer is used for sorghum planting, growth indexes and physicochemical indexes of plants are measured, and the microbial fertilizer can greatly improve the high biological yield of the sorghum plants, optimize the sorghum quality and promote the growth of the sorghum plants.

The application discloses a bacillus licheniformis longus agent, a preparation method and application, and the agent is prepared from a bacillus licheniformis longus strain C1 and comprises the following steps: culturing the bacillus licheniformis longus strain through a tryptone soy agar culture medium until yellow single colonies appear; inoculating the yellow single colonies into serum, and performing seed culture on the bacillus licheniformis longus liquid culture medium until a logarithmic growth phase is reached to obtain a bacterial liquid; inoculating the cultured bacterial liquid into a production device according to the bacillus licheniformis longus strain, and performing production culture on the bacillus licheniformis longus production culture medium until a logarithmic growth phase is reached to prepare a bacillus licheniformis longus biocontrol agent; performing centrifugal treatment on the obtained bacillus licheniformis longus biocontrol agent, taking supernatant, and performing filtration on the supernatant to prepare a bacillus licheniformis longus biocontrol fermentation liquid. The bacillus licheniformis longus agent is applied to antagonizing pyricularia oryzae and preventing and controlling rice blast.

The invention relates to the technical field of flocculant development, in particular to a bio-based flocculant and a preparation method thereof.The bio-based flocculant comprises 15%-25% of cationic polyacrylamide, 23%-57% of polymeric silicate aluminum ferric sulfate, 6%-10% of heavy metal composite adsorption aid powder, 13%-23% of a magnetic adsorption aid, 7%-13% of composite bacterial powder, 2%-5% of a microbial deodorant, 4%-9% of a microbial oxygen aid and 2%-16% of an excipient. The heavy metal composite adsorption assistant powder comprises stenotrophomonas maltophilia, providencia alcaligenes and a mixture of metallophilic bacteria and lysinibacillus, the composite bacterial powder is a mixture of agaricus blazei hyphae, lentinus edodes mushroom residues, flammulina velutipes mushroom residues and oyster mushroom residues, and the microbial oxygen assistant is a mixture of cyanobacteria chlorophycocyanobacteria and zoogloea. On one hand, the flocculation speed and the decomposition speed of heavy metal impurities in sewage can be effectively increased, on the other hand, magnetic adsorption and rapid flocculation of the heavy metal impurities in the sewage can be achieved, and meanwhile, the flocculant can also achieve increase of the oxygen content of the sewage, so that the oxygen exposure purification efficiency during sewage flocculation is effectively improved.

The invention belongs to the technical field of organic fertilizers, and particularly relates to a microbial organic fertilizer for promoting selenium enrichment of vegetables, which is prepared from the following components: composted chicken manure, composted rice straw, calcium superphosphate, potassium sulfate, a functional bacterial agent and the balance of inert filler, wherein the functional bacterial agent is prepared by mixing freeze-dried powder of follicle-like bacillus and freeze-dried powder of lysinibacillus; and the inert filler is bentonite. According to the microbial organic fertilizer disclosed by the invention, the vesicularia delavayi freeze-dried powder and the lysinibacillus lysinii freeze-dried powder are added as functional microbial agents, so that the growth of vegetable crops can be promoted, the selenium resistance and arsenic resistance of the vegetable crops are improved, and meanwhile, the selenium enrichment effect of the crops is realized. In addition, the disease resistance of vegetable crops can be improved. In conclusion, the microbial organic fertilizer disclosed by the invention is beneficial to promoting selenium enrichment of the vegetables and realizing green and healthy development of the vegetables, and has a relatively good application prospect.

The invention belongs to the field of microorganisms, and particularly relates to a method for efficiently preparing a novel carboxymethyl pachymaran derivative. The method comprises the following steps: firstly, inoculating poria cocos bacteria CGMCC 5.78 for first-stage fermentation; then, lysinibacillus fusiformis CGMCC 1.10295 is inoculated, a selenium source is added, second-stage co-culture is carried out, and the stage comprises a key high-temperature stimulation step and a subsequent pH adjusting step; and finally, separating and purifying the fermentation liquor to obtain the selenized carboxymethyl pachymaran. By means of the specific technology, the yield of the selenized carboxymethyl pachymaran and the selenium element conversion rate are remarkably increased, and the selenized carboxymethyl pachymaran has good DPPH free radical scavenging capacity and in-vitro anti-tumor activity. The process is green and efficient, and a new way is provided for development of the functional selenium polysaccharide.

The application relates to a coupling nitrogen catabolism composite microbial agent and application thereof, and belongs to the technical field of biology. The application constructs an 'ammoniation-Dirammox' coupling system by screening high-efficiency ammoniation strains (lysine bacillus L11) and direct ammonia oxidation strains (alkaligenes M8), and optimizes the co-culturing time sequence (L11 is inoculated 48 hours in advance), so that organic nitrogen is directly converted into N2. The total nitrogen removal rate in the synchronous ammoniation and nitrification medium reaches 96.84%, and the ammonia nitrogen removal rate reaches 99.37%. In actual application, the agent dosing makes the effluent ammonia nitrogen of the sewage treatment system stable at 9.44 mg / L, and the COD is reduced to 455.98 mg / L, which is significantly better than that of the traditional process. The application solves the problems of low treatment efficiency of high-ammonia-nitrogen wastewater and serious secondary pollution, and is suitable for the collaborative treatment of aged leachate and perishable garbage leachate.

The application discloses a kind of nitrobenzene high-efficiency degradation bacterial agent, preparation and application, belong to biotechnology field, the microbial inoculum of nitrobenzene wastewater treated by the present application mainly consists of lysinibacillus (Lysinibacillus sp.LBQ22-1), and is identified as Lysinibacillus sp. by 16S rDNA sequence sequencing, has been preserved in China General Microbiological Culture Collection Center, preservation number is CGMCC NO.26095, and preservation date is November 10, 2022.The nitrobenzene degradation bacterial agent of the present application can grow with nitrobenzene as sole carbon source and nitrogen source, and 1600mg / L nitrobenzene can be degraded to 2mg / L or less in 36h, and the removal rate of nitrobenzene is more than 99.5%.The bacterial preparation prepared by the present application is added to wastewater at a mass ratio of 0.5~5 ‰, and has good degradation effect on nitrobenzene contaminated water body, solves the problem of difficult biodegradation of high-concentration nitrobenzene.