42 results about "Lysinibacillus sp." patented technology

The invention discloses lysinibacillus sp. For degrading alternaria alternata toxins and application of the lysinibacillus sp., and belongs to the technical field of microorganisms, the lysinibacillus sp. Is preserved in China Center for Type Microbiological Culture Collection, the preservation address is Wuhan University, Hubei Province, and the preservation number is CCTCC No.M20241816. The invention further discloses a preparation method of the lysinibacillus sp. According to the invention, the Lysinibacillus macroides capable of effectively reducing and degrading alternaria alternata toxin is obtained for the first time, and the quality of agricultural products (including aquatic products) is favorably improved.

The invention discloses a long lysinibacillus inoculant, a preparation method and application, the inoculant is prepared from a long lysinibacillus strain C1, and the preparation method comprises the following steps: culturing the strain of the long lysinibacillus strain through a tryptone soybean agar culture medium until yellow single colonies appear; inoculating the yellow single colony into serum, and carrying out seed culture by using a long lysine bacillus liquid culture medium until a logarithmic phase to obtain a bacterial solution; and inoculating the cultured bacterial liquid into a production device according to a long lysine bacillus strain, and carrying out production culture by using a long lysine bacillus production culture medium to a logarithmic phase to prepare the long lysine bacillus biocontrol inoculant. And carrying out centrifugal treatment on the obtained biocontrol microbial inoculum of the long lysine bacillus, taking a supernatant, and filtering the supernatant to obtain the long lysine bacillus biocontrol fermentation broth. The invention further discloses application of the lysinibacillus elongate microbial inoculum in antagonism of rice blast bacteria and prevention and control of rice blast.

The invention discloses a synthetic functional bacterial agent and application of the synthetic functional bacterial agent in salt tolerance and growth promotion of wheat, the synthetic functional bacterial agent is prepared from five strains, namely, Gordonia hydrophora, Lysinibacillus oldseyi, planomicrobium okeanokites, Luteimonas huaabiensis and Brevibacterium epidermidis, and the bacterial agent has the remarkable characteristics of no antagonism among the strains, salt and alkali tolerance, high viable count and the like, and can be applied to salt tolerance and growth promotion of wheat. The germination rate, the seedling length and the root length of the spring wheat can be remarkably increased, and the seedling length and the root length of the winter wheat are also remarkably promoted. Under the condition of salt stress, the microbial inoculum can remarkably improve the activity of catalase, peroxidase and superoxide dismutase of wheat and the total content of chlorophyll, after microbial inoculum treatment, the content of malondialdehyde and proline of wheat is remarkably reduced, and the microbial inoculum plays a role in promoting and protecting spring wheat and winter wheat in the process of resisting saline-alkali stress and has a good application prospect. The method has an influence effect on agricultural development and sustainable development in the arid region.

The invention discloses a strain of Lysinibacillus bacilli B1 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653, and the preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653. The bacstein lysine bacillus has an inhibition effect on penicillium expansum, aspergillus ochraceus, botrytis cinerea, escherichia coli, listeria monocytogenes, sclerotium rolfsii, root rot bacteria, ralstonia solanacearum and fusarium oxysporum. The strain can be used for preventing and treating southern blight and root rot of siraitia grosvenorii, banana fusarium wilt, mycosis of navel oranges and the like; the bacterial fertilizer can be applied to the fields of fruit preservation and bacterial fertilizer biocontrol of the plants; meanwhile, the strain can also be applied to the field of food preservation, is a bacterial strain with multiple functions and wide application fields, and has a good application prospect.

The invention belongs to the field of resource utilization of livestock and poultry manure, and particularly relates to a thermophilic ammoniation compound microbial agent, a fertilizer and application. The thermophilic ammoniation compound microbial agent is prepared by compounding lysinibacillus spp., bacillus megatherium and white-rot fungi, the preservation number of the lysinibacillus sphaeroides is CCTCC NO: M 20242776, the preservation number of the bacillus megaterium is CCTCC NO: M 20242777, the preservation number of the white-rot fungi is CCTCC NO: M 20242778, and the three strains are all preserved in the China Center for Type Culture Collection. Tests prove that the thermophilic ammoniation compound microbial agent shows higher ammoniation activity at 60 DEG C, and can be used for promoting the compost recycling process and improving the compost product quality.

The present invention relates to a method for treating high-salt wastewater discharged into the sea, comprising five steps: ion flotation pretreatment, ozone catalytic oxidation, biochemical treatment and deep treatment; the biochemical treatment is: adding a composite salt-tolerant bacteria liquid after domestication of the first-level wastewater I to the first-level wastewater I obtained after ozone catalytic oxidation for anoxic-aerobic process treatment, the composite salt-tolerant bacteria liquid after domestication of the first-level wastewater I is obtained by inoculating lysine Bacillus, Clostridium butyricum and Hasavobacterium into a culture medium and culturing to the logarithmic phase to obtain the corresponding seed liquid, and then adding it to the domestication liquid containing the first-level wastewater I at a volume ratio of 1: (0.5-0.7): (0.3-0.6) for domestication. After the present invention compounds Hasavobacterium with lysine Bacillus and Clostridium butyricum in a certain proportion, the salt tolerance of the composite bacteria is significantly improved, thereby improving the wastewater treatment capacity, and the treated water quality reaches the fourth category seawater standard, which can be directly discharged into the sea for treatment.

The invention discloses a spindle-shaped lysinibacillus and an application thereof, and relates to the field of microbial screening and application. The deposit number of the spindle-shaped lysinibacillus is CGMCC No. 28950. The spindle-shaped lysinibacillus provided by the invention has the properties of dissolving phosphorus and potassium, promoting the growth of crops, and having a good control effect on southern root-knot nematodes.

The invention discloses a microbial premix product and application thereof in aquaculture, and belongs to the technical field of microorganisms, the microbial premix product comprises lysinibacillus and lactobacillus rhamnosus, the lysinibacillus is preserved in China Center for Type Microbiological Culture Collection, the preservation address is Wuhan University, Hubei Province, and the preservation number is CGMCC NO. The preservation number is CCTCC (China Center For Type Culture Collection) No.M20241816; the lactobacillus rhamnosus is preserved in the China Center for Type Culture Collection, the preservation address is Wuhan University, Hubei province, and the preservation number is CCTCC (China Center for Type Culture Collection) No.M20241815. The microbial premix product provided by the invention is helpful for promoting the growth of the stichopus japonicus, and can significantly reduce the cadmium content of the stichopus japonicus.

The application discloses a kind of synthetic functional microbial inoculants and its application in salt-tolerant growth promotion of wheat, adopt hydrophobic gordon's bacteria ( Gordonia hydrophobica ), odyssey lysine bacillus ( Lysinibacillus odysseyi ), sea bed motility microorganism ( Planomicrobium okeanokoites ), north China broussonetia yellow color bacterium ( Luteimonas huabeiensis ), epidermis brevibacterium ( Brevibacterium epidermidis ) five strains of bacteria, microbial inoculant has no antagonism between strains, salt-tolerant, and other significant characteristics such as viable count, can significantly promote spring wheat germination rate, seedling length and root length, and has significant promoting effect on seedling length and root length of winter wheat.Under salt stress conditions, the microbial inoculant can significantly improve the activities of catalase, peroxidase and superoxide dismutase of wheat, and the total chlorophyll content;after treatment with the microbial inoculant, the contents of malondialdehyde and proline of wheat are significantly reduced.The microbial inoculant plays a promoting and protecting role in the process of resisting salt stress of spring wheat and winter wheat, and has an impact on the development and sustainable development of arid region agriculture.

The invention discloses a composite micro-ecological preparation and application thereof in feed, and belongs to the technical field of microorganisms, the composite micro-ecological preparation comprises lysinibacillus and mannan oligosaccharide, the lysinibacillus is preserved in the China Center for Type Microbiological Culture Collection, the preservation address is Wuhan University, Hubei Province, and the preservation number is CCTCC No.M20241816. The invention further discloses a preparation method of the composite micro-ecological preparation. The compound microecological preparation disclosed by the invention can be used for more remarkably reducing the content of aflatoxin B1 and increasing the content of water-soluble polysaccharide, and is beneficial to increasing the market value of a feed containing peanut meal, corn and / or soybean meal.

The invention belongs to the field of resource utilization of livestock and poultry manure, and particularly relates to a spindle-shaped lysine bacillus, fermentation liquor, an ammonifying bacterium agent and application. The lysinibacillus fusiforme is preserved in the China Center for Type Culture Collection, and the preservation number of the lysinibacillus fusiforme is CCTCC (China Center for Type Culture Collection) NO: M 20242776. Tests prove that the ammoniating bacterium inoculant prepared by utilizing the lysine bacillus spunlaginosus shows higher ammoniating activity at 37 DEG C, and the ammoniating bacterium inoculant can promote the composting recycling process and improve the composting product quality by inoculating the ammoniating bacterium inoculant.

The invention provides lysinibacillus sphaericus WSF-65, a fumigation preparation and application of the lysinibacillus sphaericus WSF-65 and belongs to the technical field of biological prevention and control, the Latin name of the bacillus sphaericus is Lysinibacillus sphaericus, the strain number of the bacillus sphaericus is WSF-65, and the preservation number of the bacillus sphaericus is CCTCC (China Center for Type Culture Collection) NO.M20251104. The Lysinibacillus sphaericus WSF-65 disclosed by the invention can inhibit the occurrence of the citrus anthracnose through generated volatile substances, so that the Lysinibacillus sphaericus WSF-65 can be developed into a fumigation preparation for controlling the occurrence of the citrus anthracnose; the lysinibacillus sphaericus WSF-65 has a certain broad-spectrum inhibition effect on the pathogenic fungi after the citrus is picked, and fermentation liquor of the lysinibacillus sphaericus WSF-65 can be adopted to soak the picked citrus fruits, so that the rotting of the citrus fruits caused by the pathogenic fungi during the storage and transportation period after the citrus is picked is reduced; the inhibition effect of the biocontrol bacterium WSF-65 on pathogenic fungi is similar to that of a chemical bactericide commonly used in the market.

The invention relates to the technical field of microorganisms, in particular to lysinibacillus elongate E and application of the lysinibacillus elongate E in prevention and treatment of root-knot nematodes. The invention provides a microbe, namely, lysinibacillus elongate E, capable of effectively preventing and treating root-knot nematodes. The preservation number of the microbe is CGMCC (China General Microbiological Culture Collection Center) No.33877. The effect of the strain is verified through indoor nematode killing activity determination and field tests, and results show that in an indoor nematode killing activity determination experiment, the fatality rate of fermentation liquor of the long lysine bacillus E strain on nematodes is close to 100%, the effect is the best, 50% fermentation liquor still keeps very high activity (87.56%), and the fermentation liquor still keeps very high activity (87.56%). After the supernate of the fermentation liquor of the long lysine bacillus E strain is sterilized, the effect is slightly reduced from 92.38% to 88.42%; in a field test, the strain can also effectively prevent and treat tomato root knot nematode disease, and the prevention and treatment effect is 48.56%.

This invention discloses a lysinibacillus macroides bacterium that degrades Alternaria alternata toxins and its applications, belonging to the field of microbial technology. The lysinibacillus macroides is deposited at the China Center for Type Culture Collection (CCTCC), Wuhan University, Hubei Province, with accession number CCTCC No. M20241816. This invention is the first to obtain a lysinibacillus macroides bacterium that can effectively reduce and degrade Alternaria alternata toxins, which helps improve the quality of agricultural products (including aquatic products).

The application provides a lysinibacillus sphaericus for producing lipase and application thereof, and relates to the technical field of microbial enzymes. Lysinibacillus sphaericus Yin-202501, and a preservation number of CCTCC No.M2025574.The lysinibacillus sphaericus for producing lipase provided by the application has the following characteristics: Lysinibacillus sphaericus Yin-202501 has the following characteristics: high lipase activity, simple process, and the like. Lysinibacillus sphaericus Yin-202501 is used as an enzyme-producing strain, and olive oil emulsifier and ferrous sulfate are appropriately added in Luria.Bertani (LB) liquid medium, so that the method for preparing a high-activity lipase crude solution by using lysinibacillus sphaericus has the characteristics of simple operation, simple process, good repeatability and the like.

The invention belongs to the technical field of microorganisms, and particularly relates to an IAA-producing strain XY11 and application of the IAA-producing strain XY11 in inhibition of activity of heavy metal cadmium. The invention discloses a strain XY11, the taxonomic name of which is Lysinibacillus sp. The strain XY11 is preserved in Guangdong Microbial Culture Collection Center on July 1, 2025, and the preservation number of the strain XY11 is GDMCC No: 66620. According to the present invention, the morphological observation and the molecular biological identification are performed on the strain XY11, such that the strain XY11 is determined to be Lysinibacillus sp. In addition, tests find that the strain XY11 has the effects of selenium resistance, cadmium resistance, IAA production, selenium activation and cadmium reduction, can improve the content of effective selenium in soil and reduce the content of effective cadmium in soil, and is beneficial to selenium enrichment and cadmium reduction of crops; meanwhile, the growth of crops can be promoted, the selenium and cadmium stress resistance of the crops is improved, and the method has important significance for producing selenium-rich green products.

The application discloses a microbial substrate for strawberry planting and a preparation method and application thereof, and the substrate is made of peat, perlite, bacteria-loaded biochar and fungi, and the volume ratio of the peat, the perlite and the bacteria-loaded biochar is (45-75):25:(10-30); the bacteria-loaded biochar is prepared by loading biochar with a biocontrol composite bacteria; and the biocontrol composite bacteria is composed of Rhodopseudomonas palustris, Bacillus subtilis and Bacillus licheniformis. The application changes the traditional single bacterial group into a mixed bacterial group, and uses biochar as a microbial loading material, so that the mixed bacterial group can better improve the prevention and treatment effect, and the biochar can provide a carbon source for the microorganisms and promote the better reproduction and growth of the microorganisms.

The invention discloses a functional composite microbial inoculant for ecological improvement of moderate and severe saline-alkali soil and a preparation method and application thereof. The composite bacterial agent is prepared by compounding bacterial solutions of paenibacillus polymyxa SMr02, bacillus velezensis DS-R-B-3-5, bacillus laterosporus BHr6-5, lysinibacillus fusiformis SCB19 and pseudomonas japonicus D109 according to the volume ratio of 3: 2: 2: 2: 1. The complex microbial inoculant disclosed by the invention has good saline-alkali tolerance, can obviously improve the stable soil fertility and nutrient content in moderate and severe saline-alkali soil, reduce the soil salinity and alkalinity, relieve saline-alkali stress of salt-tolerant crops and promote growth of the salt-tolerant crops, is simple and convenient to operate, low in cost and environment-friendly, and has a wide application prospect. The method is suitable for ecological improvement of moderate and severe saline-alkali soil in arid areas, irrigation areas in the middle and upstream of the Yellow River and the like, and has good ecological benefits and economic benefits.

The invention discloses a multi-effect-in-one compound bacterium capable of promoting decay and growth during straw returning, which consists of lysinibacillus fusiformis H93 and bacillus rollei X96, the preservation numbers of the lysinibacillus fusiformis H93 and the bacillus rollei X96 are respectively CGMCC (China General Microbiological Culture Collection Center) NO.33633 and CGMCC NO.33632, and the compound bacterium is preserved in China General Microbiological Culture Collection Center on February 24, 2025. The invention further discloses a complex microbial inoculant prepared from the strain and application of the complex microbial inoculant in promoting degradation of wheat straw directly returned to the field and promoting growth of rice. In different rice and wheat crop rotation soil in Jiangsu 13, a bacterium of lysinibacillus fusiformis H93 is screened from Huaian soil with the best crop growth promoting effect, a bacterium of bacillus rolfsii X96 is screened from Yuzhou soil with the best straw degradation effect, and the formed compound bacteria have the effects of straw direct returning to the field to promote decay and crop growth promoting.

The invention discloses a compound flora fermentation method and application of polygonatum cyrtonema, and relates to the technical field of microbial ferment.The method comprises the steps that polygonatum cyrtonema is used as a raw material and subjected to liquid nitrogen quick-freezing and freeze-drying, grinding and screening with a 60-mesh sieve for pretreatment; according to the method, the total sugar concentration of the polygonatum sibiricum can reach 28.75 mg / mL, the polysaccharide concentration of the polygonatum sibiricum can reach 27.70 mg / mL, the yield of the polygonatum sibiricum can reach 34.63%, and the total sugar concentration, the polysaccharide concentration and the yield of the polygonatum sibiricum are obviously higher than those of single-bacterium fermentation, double-bacterium fermentation and three-bacterium fermentation; the increase of the flavone concentration of a fermentation product is superior to that of a blank group, the DPPH free radical scavenging rate reaches 73%, and the pH is reduced to 3.0 at the final stage of fermentation. The extraction rate and biological activity of effective components are improved through the synergistic effect of florae, the process is stable and controllable, and the method is suitable for the fields of food, health care products and medicines and has good application prospects.

The invention provides lysinibacillus for producing lipase and application of the lysinibacillus, and relates to the technical field of microbial enzymology. The lysinibacillus subtilis for producing the lipase is named as Lysinibacillus capsici Yin-202501, and the preservation number of the lysinibacillus capsici Yin-202501 is CCTCC (China Center for Type Culture Collection) No. M2025574. The Lysinibacillus capsici Yin-202501 disclosed by the invention has the following characteristics that the activity of producing the lipase is high, and the process is simple. According to the method, Lysinibacillus capsici Yin-202501 is used as an enzyme producing strain, a proper amount of olive oil emulsifier and ferrous sulfate are added into a Luria. Bertani (LB) liquid culture medium, the method for preparing the high-activity lipase crude solution from the lysinibacillus has the advantages of being easy to operate, simple in process, good in repeatability and the like, the prepared lipase solution is high in activity, the yield is high, and the method is suitable for industrial production. The method has good application potential and development and utilization prospects in the industrial field.

The application discloses an ethyl carbamate degrading strain and application thereof, and belongs to the field of food biotechnology. The application provides fusiform lysinibacillus ZYXW-J9-5 which can efficiently degrade ethyl carbamate, and the preservation number is GDMCC No. 64938. The fusiform lysinibacillus ZYXW-J9-5 has the advantages of fast growth speed, the ability of degrading ethyl carbamate, high production of ethyl carbamate hydrolytic enzyme, excellent fermentation performance and probiotic performance, and is a safe strain and can be applied in the field of food. The strain can be used as a fermenting agent to degrade EC in fermented food, has obvious effect of degrading EC, is convenient to use, has low production cost, and can be widely applied in fermented food such as wine beverage, fermented dairy product, soy sauce, vinegar and the like.

The invention discloses a coupled nitrogen catabolism compound microbial agent and application thereof, and belongs to the technical field of biology. According to the method, an efficient ammonification strain (lysinibacillus L11) and a direct ammoxidation strain (alcaligenes M8) are screened, an ammonification-Dirammox coupling system is constructed, the strain co-culture time sequence (L11 is inoculated 48 hours earlier) is optimized, and organic nitrogen is directly converted into N2. According to the method, the total nitrogen removal rate in the synchronous ammoniation and nitrification culture medium reaches 96.84%, and the ammonia nitrogen removal rate reaches 99.37%. In practical application, the ammonia nitrogen in effluent of a sewage treatment system is stabilized at 9.44 mg / L and COD (Chemical Oxygen Demand) is reduced to 455.98 mg / L by adding the microbial agent, which is obviously superior to that of the traditional process. The method provided by the invention solves the problems of low treatment efficiency of high-ammonia-nitrogen wastewater and serious secondary pollution, and is suitable for co-treatment of aging leachate and perishable landfill leachate.

This application discloses a lysinibacillus, its biological preparation, and its application, belonging to the field of microbial technology. The lysinibacillus of this application, deposited with CCTCC NO: M 20251099, exhibits high control efficacy against major rice pests and diseases, rice planthoppers and rice blast. Furthermore, since rice planthoppers and rice blast can occur at both the seedling and panicle stages, with overlapping occurrence phases, lysinibacillus possessing both rice planthopper and rice blast bioinhibitory activity can effectively reduce the frequency of pest and disease control efforts and the investment in control products.

The invention relates to the technical field of microorganisms, in particular to lysinibacillus sphaericus Y-WFX-3 and application thereof.The lysinibacillus sphaericus Y-WFX-3 is a microorganism which is screened by the applicant and has the antibacterial property, the microorganism belongs to bacillus, and according to detection of pathogenic bacteria, the strain has the high inhibition effect on most vibrio and can be used for preparing an antibacterial agent. Wherein the bacterial strain comprises vibrio candersonii, vibrio alginolyticus, vibrio magic and / or vibrio parahaemolyticus, it is indicated that the bacterial strain can effectively prevent fish diseases caused by vibrio, enough germplasm resources can be provided for later preparation of antibacterial microecological preparation products, and in addition, through research on the influence of waste pericarp on the growth of microorganisms, the bacterial strain can be used for preventing fish diseases caused by vibrio. A new thought of kitchen waste utilization is obtained, and the corresponding kitchen waste is applied to dry powder processing of the strain, so that the raw material cost is reduced, the application, transportation and storage capacities of the strain are improved, and a new processing method is provided for marketization of the strain.

The invention discloses a microbial substrate for strawberry planting and a preparation method and application thereof, the substrate is prepared from the following raw materials: peat, perlite, bacterium-loaded charcoal and fungi, and the volume ratio of the peat to the perlite to the bacterium-loaded charcoal is (45-75): 25: (10-30); the bacterium-loaded biochar is prepared by loading biocontrol compound bacteria on biochar; the biocontrol compound bacteria are prepared from rhodopseudomonas palustris, bacillus subtilis and lysine bacillus. The traditional single flora is changed into the mixed flora, the biochar is used as a microorganism loading material, the mixed flora can better improve the prevention and treatment effect, and the biochar can also provide a carbon source for microorganisms and promote the microorganisms to better breed and grow.

The invention discloses lysinibacillus, lysinibacillus high-density fermentation liquor and a preservation method of the lysinibacillus high-density fermentation liquor. The lysine bacillus disclosed by the invention can be used for efficiently synthesizing auxin indoleacetic acid (IAA) and has good saline-alkaline resistance, and tests show that the strain and fermentation liquor thereof have a remarkable promoting effect on plant growth; by optimizing the fermentation process of the lysinibacillus, the thallus density and the IAA yield can be remarkably improved, the high-density fermentation liquor of the lysinibacillus can be obtained, the unification of high-density fermentation and high-activity metabolites is realized, the process is stable, and the repeatability is good. The lysinibacillus high-density fermentation liquor is further subjected to special treatment and is matched with a specific composite protective agent, so that the vitality of thalli can be effectively maintained, the normal-temperature storage life of the fermentation liquor is remarkably prolonged, and the problems of short shelf life and poor storage and transportation stability of a liquid microbial inoculum are solved.

The invention belongs to the technical field of microorganisms, and particularly relates to a selenium-tolerant cadmium-tolerant strain XY10 and application thereof in IAA production. The invention relates to a strain XY10, the taxonomic name of which is Lysinibacillus macrorrhizus, and the strain XY10 is preserved in Guangdong Microbial Culture Collection Center on July 1, 2025, and the preservation number is GDMCC No: 66619. According to the invention, the strain XY04 is subjected to morphological observation and molecular biological identification, and it is determined that the strain XY10 is lysinibacillus macroides, and the strain XY04 and the strain XY10 are lysinibacillus macroides. In addition, tests find that the strain XY10 has the effects of selenium resistance, cadmium resistance, IAA production, selenium activation and cadmium reduction, can improve the content of effective selenium in soil and reduce the content of effective cadmium in soil, and is beneficial to selenium enrichment and cadmium reduction of crops; meanwhile, the growth of crops can be promoted, the selenium and cadmium stress resistance of the crops is improved, and the method has important significance for producing selenium-rich green products.

The present invention relates to a dental self-healing resin composite based on biomineralization, its preparation and application. The dental self-healing resin composite includes: a base resin, a filler, a nutrient powder, and a bacterial powder; wherein the bacteria in the bacterial powder are selected from at least one of Bacillus, Bifidobacterium longum, or Lactobacillus reuteri; the Bacillus is selected from at least one of Bacillus sphaericus, Lysinibacillus sphaericus, Bacillus licheniformis, Bacillus pasteurii, or Bacillus subtilis; the preparation method includes: mixing the base resin, barium glass powder, nutrient powder, and bacterial powder, and subjecting it to photocuring to obtain. Compared with the prior art, the present invention introduces bacteria that can induce calcium carbonate precipitation into the dental resin composite, and achieves the purpose of self-healing through biomineralization, and realizes multiple self-healings.

The invention relates to lysinibacillus sp.GWN-3 and application thereof in inhibiting sulfate reducing bacteria in the technical field of biological treatment of oilfield produced water, the lysinibacillus sp.GWN-3 is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO 13904, the preservation date is March 20, 2017, and the preservation number is CGMCC NO 13904. The lysinibacillus fusiformis is classified and named as lysinibacillus fusiformis, and is named as Lysinibacillus fusiformis in Latin literature. The 16S rDNA (ribosomal deoxyribonucleic acid) gene sequence of the lysinibacillus sp. GWN-3 is as shown in SEQ ID NO: 1. The lysinibacillus sp. Is separated from oilfield produced water, is used for inhibiting proliferation of high-concentration SRB in an oilfield produced water ground treatment system, is long in inhibition time, and has a relatively good biological inhibition effect on SRB at the rear end of the oilfield ground produced water treatment system compared with a chemical sterilization technology; the content of SRB at the rear end of the system is effectively controlled, and the treatment cost of produced water is greatly reduced.