A kind of compound microbial fertilizer special for heterophylla heterophylla and its preparation method and application

A technology of composite microorganisms and composite microbial agents, which is applied in the fields of agricultural fertilizers and biological control, can solve problems such as the inability of beneficial bacteria to colonize, and achieve the effects of good rhizosphere colonization ability, improved soil physical and chemical properties, and stable efficacy

Active Publication Date: 2019-05-03
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies also found that continuous cropping of Pseudostellariae Pseudostellariae led to significant changes in the soil environment, such as acidification, and Pseudostellariae Pseudostellaria continued to release a variety of secondary metabolites and self-toxic substances. Colonizes in the rhizosphere of Pseudostellariae Pseudostellariae, so the host specificity of soil-borne pathogens of Pseudostellariae Pseudostellariae and the particularity of continuous cropping soil environment should be considered comprehensively when using microbial agents to control the replanting diseases of Pseudostellariae Pseudostellariae

Method used

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  • A kind of compound microbial fertilizer special for heterophylla heterophylla and its preparation method and application
  • A kind of compound microbial fertilizer special for heterophylla heterophylla and its preparation method and application
  • A kind of compound microbial fertilizer special for heterophylla heterophylla and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Screening and identification of indigenous antagonistic bacteria

[0034]Pseudomonas selective medium and Bacillus selective medium were used to isolate Pseudomonas and Bacillus from the rhizosphere of Radix Pseudostellariae, and inorganic medium (containing higher concentration of autotoxic substances in Pseudostellariae) was used to isolate Toxin-degrading bacteria were isolated.

[0035] Randomly selected single colonies on the above-mentioned different media were used to face off on PDA plates to evaluate their antagonistic effects on heterophylla-specific pathogenic bacteria (such as Fusarium oxysporum, Fusarium moniliforme, and ankle mold, etc.). Bacteria with strong antagonistic ability were identified by 16s rRNA gene or 16s-23s rRNA interval PCR identification for molecular identification. 16s rRNA gene PCR amplification upstream and downstream primers are: 27f (5'-AGAGTTTGATCCTGGCTCAG-3') and 1522r (5'-AAGGAGGTGAT CCAGCCGCA-3'); 16s-23s rRNA gene PC...

Embodiment 2

[0045] Embodiment 2 Preparation of special compound microbial fertilizer

[0046] The special compound microbial fertilizer of the present invention is made by fermenting the special compound microbial agent and organic materials together. Among them, the special compound microbial agent is made by mixing the common beneficial bacteria solution (EM composite bacteria solution) and the indigenous beneficial bacteria solution (IM composite bacteria solution) in a volume ratio of 4:6. The specific preparation method is as follows:

[0047] 2.1 Preparation of EM composite bacterial solution

[0048] Add 300g of brown sugar and 50mL of MS mother liquor to 18L of well water (tap water needs to be left for at least 2 days before use), then add 1L of activated commercially available EM liquid bacteria, and place it in a room at 37°C for 3 weeks to ferment in a closed room. From the second day onwards, you can open the airtight container mouth to deflate and reduce pressure once ever...

Embodiment 3

[0061] Example 3 Evaluation of Field Effects of Special Compound Microbial Fertilizers on Prevention and Control of Pseudostellaria Pseudostellariae Continuous Cropping Obstacles

[0062] First, use the prepared special compound microbial fertilizer as base fertilizer to pre-improve the soil for continuous cropping of Pseudostellariae Pseudostellariae, then prepare the plots and plant Pseudostellariae Pseudostellariae, and from the seedling stage of Pseudostellariae Pseudostellariae, root-irrigating and topdressing special compound microbial liquid used for fermented fertilizer Bacteria, the difference in yield was compared at the harvest stage. The specific preventive measures are as follows:

[0063] 3.1 Preparation of microbial organic base fertilizer

[0064] Mix the organic materials bean dregs, rice bran, dried pig manure, fish meal, and brown sugar in a weight ratio of 2:1:1:1:0.03, and then add a special compound microbial liquid bacterial agent (1L / 10kg) to control t...

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Abstract

The invention relates to a special composite microbial fertilizer for controlling successive cropping obstacles of radix psuedostellariae. The fertilizer is prepared by fermenting a special composite microbial inoculant and organic materials (bean pulp, rice bran, dried pig manure, fish meal and brown sugar in a weight ratio of 2:1:1:1:0.03). The composite microbial inoculant is prepared by mixing a universal beneficial bacterium solution and an aboriginal beneficial bacterium solution in a volume ratio of 4:6. The aboriginal beneficial bacterium comprises an aboriginal beneficial bacterium composite bacterium solution which is formed by independently fermenting Burkholderia, pseudomonad and Bacillus separated to the radix psuedostellariae rhizosphere soil, and mixing in a volume ratio of 4:1:1. The organic materials are subjected to special composite microbial inoculant anaerobic fermentation and oxygen-consuming fermentation to prepare the special composite microbial fertilizer. The long-term on-site verification proves that the microbial fertilizer has the advantages of reasonable formula, stable effects, favorable biocontrol effect and no pollution, has obvious effects on controlling succession cropping obstacles of radix psuedostellariae, is safe and ecological, and thus, has wide development and application prospects.

Description

technical field [0001] The invention relates to a special compound microbial fertilizer for heterophylla and its preparation method and application, which can be used to prevent and cure the continuous cropping obstacle of medicinal plants heterophylla, and belongs to the technical field of agricultural fertilizers and biological control. Background technique [0002] Pseudostellaria ( Pseudostellaria heterophylla ) is also known as children's ginseng and baby ginseng. It is a perennial herbaceous plant of Caryophyllaceae. Its roots are used as medicine. However, there are serious continuous cropping obstacles in the process of planting Pseudostellariae heterophylla, also known as the problem of repeated cropping or replanting disease, that is, the same plot of Pseudostellaria heterophylla has been planted for many years, resulting in the failure of normal expansion of the root of Pseudostellaria heterophylla, serious pests and diseases, and a significant decline in yield an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05F17/00C05G3/04A01C21/00C12N1/20C12N1/16C12R1/40C12R1/39C12R1/38C12R1/07C12R1/125C12R1/085C12R1/11C05G3/80
CPCA01C21/00C05F1/005C05F17/50C05G3/80C12N1/16C12N1/20C05F3/00C05F5/002C05F11/00C05F11/08Y02W30/40
Inventor 吴林坤林文雄陈军吴红淼林生
Owner FUJIAN AGRI & FORESTRY UNIV
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