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Compound microbial agent for relieving continuous cropping obstacles of melon crops and application

A compound bacterial agent and continuous cropping obstacle technology, which is applied in the field of microbial and continuous cropping obstacle restoration, can solve problems such as continuous cropping obstacles, and achieve the effects of promoting stress resistance, improving soil physical and chemical properties, and good colonization ability

Inactive Publication Date: 2018-09-28
SHENYANG AGRI UNIV
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Problems solved by technology

[0012] In order to make up for the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a compound bacterial agent and its application for alleviating continuous cropping obstacles of melon crops, for degrading autotoxic substances in the rhizosphere of melon crops, and preventing and treating melon wilt bacteria and other soil pathogenic fungi to solve the problem of continuous cropping obstacles of melon crops, and to promote plant growth and degrade autotoxicity

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  • Compound microbial agent for relieving continuous cropping obstacles of melon crops and application
  • Compound microbial agent for relieving continuous cropping obstacles of melon crops and application

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[0020] 1. Materials and Methods

[0021] 1.1 Preparation of compound bacterial agent

[0022] (1) The medium used to isolate Pseudomonas oryzae is an inorganic salt medium, and the formula includes MgSO 4 ·7H 2 O0.2g / L, CaCl 2 2H 2 O 0.02g / L, FeCl 3 0.05g / L, KH 2 PO 4 1.0g / L, K 2 HPO 4 1.0g / L, NH 4 NO 3 1.0g / L, cholesterol 0.3g / L, and agar 17g / L respectively with a treatment concentration of 200μg·mL -1 The p-hydroxybenzoic acid, benzoic acid and cinnamic acid are used as the sole carbon source, and the pH value is adjusted to neutral.

[0023] The method for isolating Pseudomonas oryzae: wash the melon rhizome junction with running water, cut it into small pieces, soak in 75% alcohol for 1min, rinse with 0.1% mercury liter for 30s, and rinse it with sterile water for 4 times. Grind it into a slurry in a bowl, dilute the grinding solution 3 times, spread it on an inorganic salt medium, and cultivate it in an incubator at 28°C for 48 hours to obtain a strain of P...

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Abstract

The invention relates to a compound microbial agent for relieving continuous cropping obstacles of melon crops and application. The compound microbial agent is composed of pseudomonas oryzihabitans and trichoderma according to a ratio of 1:3-3:1, the concentration of the pseudomonas oryzihabitans in bacterial liquid is 1.0-3.0x107cfu / ml, and the concentration of trichoderma in the bacterial liquidis 1.0-3.0x107cfu / mL. The application method of the composite microbial agent comprises the steps that an application method of hole application or root irrigation is adopted in the transplanting period of melon crops or in the flowering and fruit setting period of the melon crops, and the dosage of the microbial agent for each seedling is 250ml. The composite microbial agent is a mixed bacteriaof melon plant rhizosphere soil separating bacteria and trichoderma probiotics, has good colonizing ability in the rhizosphere of the melon crops, is safe and reliable, has a good degradation effect on autotoxins in soil of continuous cropping melon crops, obviously improves the physical and chemical properties of the soil, and has an obvious promotion effect on the stress resistance of the meloncrops.

Description

technical field [0001] The invention relates to the field of repairing microorganisms and continuous cropping obstacles, in particular to a composite bacterial agent for alleviating continuous cropping obstacles of melon crops and its application. Background technique [0002] Continuous cropping obstacle (also known as replanting disease) refers to the phenomenon that the same crop or the same family of crops are planted continuously, even under normal management conditions, the growth potential will be weakened, the yield will be reduced, the quality will be reduced, and the pests and diseases will be aggravated. Continuous planting of crops leads to aggravation of soil-borne diseases. The survey on the causes of vegetable continuous cropping obstacles found that soil-borne diseases were the dominant factor, accounting for 57%, second only to underground nematodes at 63%. The other factor was poor physical and chemical properties of soil at 14.2%. The causes of continuous ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14A01G7/06C12R1/38C12R1/885
CPCA01G7/06C12N1/14C12N1/20C12N1/205C12R2001/38
Inventor 高增贵姚远刘畅刘限孙艳秋李天亚
Owner SHENYANG AGRI UNIV
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