37 results about "Pseudomonas oryzihabitans" patented technology

Methylotrophic or methanotrophic bacteria such as Methylobacterium are transformed with a gene of interest, and expression of the gene is regulated by means of a cumate repressor protein and an operator sequence which is operatively linked to the gene of interest, and the addition of an external agent. Specifically, the cymR repressor and cmt operator from Pseudomonas putida may serve to regulate gene expression in methylotrophic or methanotrophic bacteria with the addition of cumate.

The invention provides a pathogen inspection reagent, and concretely discloses a primer probe combination and a kit for combined inspection of 15 kinds of respiratory tract infection pathogens. By aiming at specific target sequences of the gene sequence conserved region of klebsiella pneumoniae, haemophilus influenzae, streptococcus pyogenes, staphylococcus aureus, escherichia coli, chlamydia pneumoniae, mycobacterium tuberculosis, stenotrophomonas maltophilia, baumanii, mycoplasma pneumoniae, enterococcus faecalis, ligionella pneumohpillia, streptococcus pneumoniae, bacillus pyocyaneus and mycobacterium abscessus, primers and probes which do not have mutual crossed reaction are designed, so that the problem that detection probes of different pathogens can easily generate mutual influenceor interference is solved; the combination and matching of different primers and probes are ensured; the goal of simultaneously performing efficient specific inspection at the same temperature can also be achieved.

The invention discloses a kitchen waste composite microbial degradation agent as well as a preparation method and application thereof. The degradation agent is prepared from Bacillus subtilis, Streptomyces thermodiastaticus, Bacillus stearothermophilus, Lactobacillus acidophilus, Saccharomyces cerevisiae, Enteromorpha protease, Methylobacterium radiora, Pantoea dispersa, Pseudomonas oryzihabitans,Citrobacter freundii and Staphylococcus cohnii. The mixed strain accounts for 15-25% of the total mass of the degradation agent, the mass percentage of water in the degradation agent is 10-20%, the pH value of the degradation agent is 5.5-8.5, and each gram of the degradation agent contains 106-109 viable bacteria. The degradation agent has the beneficial effects that: kitchen waste containing various substances such as vegetables, grains and meat can be effectively degraded, the activity of microorganisms in the degrading agent is high, the degrading rate reaches 80% or above, the degradingrate is high, the use amount is small when the kitchen waste is treated, generated peculiar smells are small, the cost is low, use is convenient, toxic and harmful substances are not generated, and safety, reliability and environment friendliness are achieved.

The invention discloses a fish protein polypeptide biological bacterial fertilizer, which is composed of the following raw materials in parts by weight: 15-17 parts of deep sea fish, 4-6 parts of bean cake powder, 7-9 parts of rice bran, 6-8 parts of molasses, 0.045-0.05 part of strain and 50-60 parts of water, wherein the strain comprises saccharomyces cerevisiae and rhodopseudomonas palustris of which bacteria amount ratio is (1-12):(0.8-1.0). The beneficial effects of the fish protein polypeptide biological bacterial fertilizer are as follows: (1) quick action: after the fertilizer is used for 2-3 days, new roots are obviously increased, fruits are remarkably enlarged with bright color and lustre and deep green leaves; (2) the fertilizer can be used for improving fruit setting rate, reducing deformity fruits, improving single fruit weight, and realizing completely filled fruits and excellent mouthfeel; (3) the fertilizer can effectively relieve root senescence, soil compaction and salinization of soil caused by long-term fertilization of a fertilizer, and strengthen the capability of continuous cropping resistance, disease resistance and cold tolerance of crops; and (4) the fertilizer is fertilized to improve the use ratio of the fertilizer.

The invention relates to an application of pseudomonas chlororaphis subsp.aureofaciens in preventing and controlling nematode. The pseudomonas chlororaphis subsp.aureofaciens is chlororaphis subsp.aureofaciens GDMCC NO. 60273. The invention relates to the application of pseudomonas chlororaphis subsp.aureofaciens in preparing a biological bacteriostatic agent for preventing and controlling nematode. The biological bacteriostatic agent includes the chlororaphis subsp.aureofaciens served as an active ingredient and a carrier. The biological bacteriostatic agent is chlororaphis subsp.aureofaciensfermentation liquor. A method for preventing and controlling nematode comprises the step of spraying the biological bacteriostatic agent containing chlororaphis subsp.aureofaciens in claim 1 and / or fermentation liquor thereof onto an affected part. According to the application of pseudomonas chlororaphis subsp.aureofaciens in preventing and controlling nematode provided by the invention, chlororaphis subsp.aureofaciens used for preventing and controlling nematode is free from pesticide residue, free from environmental pollution and safe to human and livestock. Application of pseudomonas chlororaphis subsp.aureofaciens in preparing the biological bacteriostatic agent for preventing and controlling nematode is characterized by simple preparation process, low cost, excellent effect and convenience in use.

The invention relates to the technical field of microorganisms, in particular to a strain of pseudomonas having significant antagonistic action against Xanthomonas oryzae and magnaporthe oryzae and application of the pseudomonas. The strain is isolated from rice rhizosphere soil of Fengxian District, Shanghai; the pseudomonas provided by the invention was deposited in the China Center for Type Culture Collection on May 7, 2018, the preservation name is Pseudomonas mosselii 923, and the preservation number is CCTCC No:M 2018252. The pseudomonas provided by the invention has a significant inhibitory effect on Xanthomonas oryzae, has relatively strong inhibitory capacity against other plant pathogenic bacteria and magnaporthe oryzae of Xanthomonas, and has biological control application value.

The invention aims to provide a multiple PCR detection kit and a method for identifying susceptible bacteria species for intracranial post-operation to eliminate endogenous nucleic acid contamination. The detected bacteria species are the following common bacteria: staphylococcus aureus, enterococcus faecalis, streptococcus pneumoniae, escherichia coli, bacillus pyocyaneus, klebsiella pneumoniae and acinetobacter baumannii. Meanwhile, the invention provides the method to eliminate the endogenous nucleic acid contamination in a raw material of a PCR system and exclude a false positive experimental result of a PCR caused by the endogenous nucleic acid contamination. The detecting method using the kit has the advantages of that the operation is simple and fast, the detection cost is lowered, the detection result is accurate, and the false positive result is reduced. A negative control and a positive control are added in the amplification step, so that the judgment of false positive and false negative is effectively improved, and the detection accuracy is higher.

The invention discloses a post-neurosurgical intracranial bacterial infection common pathogenic bacterium MLPA detection probe and an application thereof. The probe comprises a first group of probes to a seventeenth group of probes, and the first group of probes to the seventeenth group of probes are probes for detecting Neisseria meningitides, Bacillus pyocyaneus, Salmonella, Stenotrophomonas maltophilia, Staphylococcus aureus, Staphylococcus epidermidis, Klebsiella pneumonia, Serratia marcescens, Acinetobacter lwoffii, Corynebacteria diphtheria, Streptococcus mitis, Acinetobacter baumannii, Streptococcus pneumonia, Propionibacterium acnes, Listeria monocytogenes, Enterobacter cloacae and Haemophilus influenza sequentially. The probe is developed through an MLPA technology, can rapidly diagnose post-neurosurgical intracranial bacterial infection 17 common pathogenic bacteria once, provides possibility for rapid diagnosis of pathogenic bacteria of like patients, and is helpful for reducing the fatality and the disability rate of like infection.

The present invention discloses a pseudomonas arsenicoxydans and an application thereof, and relates to the pseudomonas arsenicoxydans and the application thereof. The pseudomonas arsenicoxydans is pseudomonas arsenicoxydans Y24-2 and stored in China General Microbiological Culture Collection Center, a preservation number is CGMCC No.16655, and a preservation date is October 29, 2018. When the pseudomonas arsenicoxydans is in a low-temperature and oligotrophic condition and a nitrate concentration in water is 20-100 mg / L, the pseudomonas arsenicoxydans can efficiently remove nitrite in groundwater and has a removal rate of the nitrate of 12.94-93.96 mg / L / h. The pseudomonas arsenicoxydans is applied to the technical field of environmental microorganisms.

The invention discloses a molecular beacon probe for rapidly detecting urinary tract infection bacteria. The molecular beacon probe is Beacon NL. The nucleotide sequence of the probe is Beacon NL:5'-CY3-TTCCACACAAGCGGTGGAGCATGTGGTTTGGAA-BHQ1- 3'; the 5' terminal of the probe is marked with CY3, the 3' terminal of the probe is marked with BHQ1, the excitation wavelength of a fluorescent group is 552 nm, and the detection wavelength is 570nm. The molecular beacon probe uses16s rRNA of the bacteria as target spots and can detect six kinds of urinary tract infection bacteria, namely escherichia coli, enterococcus, pseudomonas aeruginosa, proteus mirabilis, klebsiella pneumoniae and staphylococcus. The probe is high in fluorescence intensity and specificity. The invention further discloses a kit and method for rapidly detecting the urinary tract infection bacteria.

The invention relates to a compound microbial agent for relieving continuous cropping obstacles of melon crops and application. The compound microbial agent is composed of pseudomonas oryzihabitans and trichoderma according to a ratio of 1:3-3:1, the concentration of the pseudomonas oryzihabitans in bacterial liquid is 1.0-3.0x107cfu / ml, and the concentration of trichoderma in the bacterial liquidis 1.0-3.0x107cfu / mL. The application method of the composite microbial agent comprises the steps that an application method of hole application or root irrigation is adopted in the transplanting period of melon crops or in the flowering and fruit setting period of the melon crops, and the dosage of the microbial agent for each seedling is 250ml. The composite microbial agent is a mixed bacteriaof melon plant rhizosphere soil separating bacteria and trichoderma probiotics, has good colonizing ability in the rhizosphere of the melon crops, is safe and reliable, has a good degradation effect on autotoxins in soil of continuous cropping melon crops, obviously improves the physical and chemical properties of the soil, and has an obvious promotion effect on the stress resistance of the meloncrops.

The invention relates to a biofertilizer leavening agent for improving a strawberry rhizosphere soil ecological structure. The biofertilizer leavening agent is characterized by comprising 40-60 partsof pseudomonas oryzihabitans, 20-30 parts of saccharomycetes, and 20-30 parts of bacillus subtilis. The pseudomonas oryzihabitans is preserved in China General Microbiological Culture Collection Center (CGMCC) which is located in No.3, No.1 courtyard, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC No.15325, and the preservation data is Jan. 29, 2018. Bacterial manures prepared by the leavening agent can better colonize at the root of the strawberry, is safe and reliable, can decompose autotoxin in soil of continuous cropping strawberries, improves physical andchemical properties of soil remarkably, promotes stress tolerance of strawberry plants, increases yield and improves quality of strawberries.

The invention provides a microbial biocontrol preparation. The microbial biocontrol preparation is a liquid and comprises active ingredients of Trichoderma longibrachiatum and its fermentation metabolites, bacillus amyloliquefaciens and its fermentation metabolites, Rhodopseudomonas palustris and its fermentation metabolites, berberine and chitosan. The microbial biocontrol preparation has a berberine concentration of 0.1 to 0.8 wt% and a chitosan concentration of 0.5-1wt%. The total viable count of the Trichoderma longibrachiatum, bacillus amyloliquefaciens and Rhodopseudomonas palustris is greater than or equal to 5*10<7> cfu / ml. The active ingredients belong to different action mechanisms. Under the synergistic effect of the active ingredients, the microbial biocontrol preparation has good prevention effects on tea grey blight. The active ingredients are environmentally friendly and non-toxic and haves pharmacological and fertilizer dual biological regulation functions.

The invention discloses a degrading bacterium for autotoxic substances of the rhizosphere of a melon crop and application of the degrading bacterium. The degrading bacterium is Pseudomonas oryzihabitans, the preservation unit is General Microbiological Center of China Committee for Culture Collection of Microorganisms (CGMCC); the address is No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing; the preservation number is CGMCC No. 15325; the preservation date is 29th January, 2018. The degrading bacterium can be used to prepare a microbial inoculum, and the concentration of the bacterial liquid is 2.0-3.0*10<8> cfu / ml. The microbial inoculum has good rhizosphere colonization ability and is safe and reliable when applied in a field, and has no pathogenic infection ability to intercropping crops, has a good degradation effect on the autotoxic substances in root exudates of the melon crop, promotes the anti-reverse ability of plants of the melon crop, improves the yield and fruit quality of the melon crop; the preparation method is simple, the microbial inoculum is reasonable in formula and stable in efficacy, and has an obvious alleviating effect on the autotoxic effectof the melon crop.

Methylotrophic or methanotrophic bacteria such as Methylobacterium are transformed with a gene of interest, and expression of the gene is regulated by means of a cumate repressor protein and an operator sequence which is operatively linked to the gene of interest, and the addition of an external agent. Specifically, the cymR repressor and cmt operator from Pseudomonas putida may serve to regulate gene expression in methylotrophic or methanotrophic bacteria with the addition of cumate.

The invention relates to a corn continuous-cropping obstacle restoration agent based on allelopathy autotoxicity and application of the corn continuous-cropping obstacle restoration agent. The corn continuous-cropping obstacle restoration agent is characterized by being prepared from pseudomonas oryzihabitans and burkholderia according to a ratio of (1:3) to (3:1), the concentration of the pseudomonas oryzihabitans in a restoration agent bacteria solution is 2.0 to 3.0*10<8>cfu / ml, and the concentration of the burkholderia in the restoration agent bacteria solution is 2.0 to 3.0*10<8>cfu / ml. The application method of the restoration agent comprises the following steps: adopting a seed coating method, stirring and sufficiently mixing 50g of corn seeds and 5mL of fermented solution, standingfor 3 to 5 hours, and then seeding. The restoration agent adopted by the invention is pseudomonas oryzihabitans and burkholderia, and is good in corn rhizosphere fix planting capability, safe and reliable, good in degradation effect for an autotoxic substance in the continuous cropping corn soil, apparent in effect for promoting the stress resistance of the corn, and capable of increasing the yield of the corns and improving the fruit quality; and the restoration agent is simple in preparation method, reasonable in formula, and capable for controlling the corn continuous cropping obstacles.

The invention discloses a free living nitrogen fixing bacterium pseudomonas oryzihabitans MBC4. The preservation number of the free living nitrogen fixing bacterium pseudomonas oryzihabitans MBC4 is CGMCC No.10820, the preservation date is May 19, 2015, and the preservation institution is China General Microbiological Culture collection Center. The strain can still live, breed and have nitrogen fixation activity under drought, large temperature differences, salt and alkali disasters and strong ultraviolet radiation. A microbial agent prepared from the free living nitrogen fixing bacterium pseudomonas oryzihabitans MBC4 can be applied to ecological systems with drought, large temperature differences, salt and alkali disasters and strong ultraviolet radiation, can increase nitrogen accumulation in the ecological systems and can perform the function of improving soil environments and the physiological activity of microorganisms and plants and increasing net primary productivity.

The invention relates to a microbial agent for preventing and treating strawberry root rot. The microbial agent comprises a Pseudomonas oryzihabitans bacterial solution and a synergist, wherein Pseudomonas oryzihabitans is collected by the General Microorganisms Center of China Committee for Culture Collection of Microorganisms, which is located at Institute of Microbiology, Chinese Academy of Sciences, 3#, 1# Courtyard, Beichen West Road, Chaoyang District, Beijing, on January 29, 2018 and has the collection number of CGMCC No. 15325; and the synergist is a 4,000-5,000-solution mixed solutionof chlorothalonil and Hymexazol. The bacterial fertilizer has good colonization capability at rhizosphere of strawberries, is safe and reliable and plays a good role in degrading root rot pathogenicbacteria in continuous cropping strawberry soil, so that the physicochemical properties of the soil are obviously improved, the stress resistance of strawberry plants is promoted, and the yield and fruit quality of strawberries are improved.

The invention discloses a compound bacterial agent for efficiently degrading kitchen waste and a preparation method thereof. The compound bacterial agent is prepared from pseudomonas, enterococcus andenterobacter, wherein the pseudomonas is aerobic bacteria, and both the enterococcus and the enterobacter are facultative anaerobic bacteria. Two types of facultative anaerobic bacteria are added asauxiliary components, wherein the enterococcus undertakes the main degradation work in the case of sufficient oxygen, and the enterobacter exerts the main degradation work in the micro-environment inthe case of local hypoxia, thereby making up for the functional defects caused by singly using aerobic bacteria, and greatly enhancing the waste treatment efficiency.

The invention discloses a prokaryotic expression vector pET28a-PADH for efficiently expressing Pseudomonas putida glutathione-independent formaldehyde dehydrogenase protein. The vector is a prokaryotic expression vector containing a Pseudomonas putida glutathione-independent formaldehyde dehydrogenase gene (PADH). In the invention, the PADH gene is cloned from Pseudomonas putida, and the expression of the PADH gene in colon bacillus is controlled by using a T7 promoter; 40 percent of expressed recombinant protein exists in a supernate in a soluble mode (accounting for 30 percent of soluble total protein of the colon bacillus), and 60 percent of expressed recombinant protein exists in an inclusion body; the high-purity PADH recombinant protein can be easily obtained from the upper inclusion body through tapping and purification and can be used as an antigen used for preparing a PADH specific antibody. The PADH recombinant protein with activity can be easily purified from the soluble total protein of the colon bacillus by affinity chromatography and is used for the production and the physiological-biochemical characteristic analysis of PADH enzyme preparations.

The invention discloses a strain of pseudomonas oryzihabitans capable of secreting ferulic acid esterase and application of the pseudomonas oryzihabitans and belongs to the technical field of fermentation engineering and biology. The pseudomonas oryzihabitans Y-5 is preserved in the China Center for Type Culture Collection in July, 13th, 2018, and the preservation number is CCTCC NO:M2018480. Thestrain can secrete ferulic acid esterase, and under the induction of bran, the enzyme activity of the ferulic acid esterase of a fermented crude enzyme liquid of the strain is 116 U / L, and by adoptingthe bran for substrate fermentation to produce ferulic acid, the yield of ferulic acid can reach 59.51+ / -0.6 mg / L. According to the pseudomonas oryzihabitans, the content of ferulic acid in yellow rice liquor can be increased when the alcoholic strength, pH value and reducing sugar content of the yellow rice liquor are not influenced, and after fermentation of strengthened wheat koji to which thepseudomonas oryzihabitans is inoculated, the content of ferulic acid in the yellow rice liquor is about 2.6 times higher than that of common yellow rice liquor fermented by traditional wheat koji.

The invention discloses a strain of pseudomonas producing ferulic acid esterase and application thereof, belonging to the fields of fermentation engineering and biotechnology. The Pseudomonas oryzihabitans Y‑5 of the present invention was deposited in the China Center for Type Culture Collection on July 13, 2018, with the preservation number CCTCC NO: M 2018480. The strain can secrete ferulic acid esterase. Under the induction of bran, the ferulic acid esterase activity of the fermented crude enzyme solution is 116U / L, and ferulic acid is produced by fermentation with wheat bran as the substrate, and the yield can reach 59.51±0.6mg / L. The Pseudomonas of the present invention can increase the content of ferulic acid in the rice wine without affecting the alcohol content, pH value and reducing sugar content of the rice wine. The content of ferulic acid is about 2.6 times that of traditional wheat koji.

The invention provides a frozen microorganism soil mixing pile. Soft soil is forcibly mixed with a water reducing agent, a bacterial liquid and a cementing solution on site in a foundation, so that the soft soil is hardened into foundation soil; and then a freezing method is implemented, so that the phenomena of frost heaving and thaw collapse of surrounding stratums can be effectively inhibited, and the bearing capacity, the shear strength, the pile body strength and the impermeability of the microorganism soil mixing pile are enhanced. According to the frozen microorganism soil mixing pile, soft soil gaps are filled with the cementing solution, and the bacterial liquid containing bacillus pasteurii and pseudomonas oryzihabitans is added, so that cracks generated when the mixing pile is frozen are repaired, and the bearing capacity of the mixing pile is improved; and by adding the water reducing agent, the impermeability of the microorganism soil mixing pile is improved, and the influence of water on the freezing effect is reduced.

The present invention aims at providing a mucosal vaccine composition that can be administered to an intraoral mucous membrane, ocular mucous membrane, ear mucous membrane, genital mucous membrane, pharyngeal mucous membrane, respiratory tract mucous membrane, bronchial mucous membrane, pulmonary mucous membrane, gastric mucous membrane, enteric mucous membrane, or rectal mucous membrane, that is useful as a prophylactic or therapeutic agent for infectious diseases or cancers, and is capable of safely and effectively inducing the systemic immune response and mucosal immune response. The present invention provides a mucosal vaccine composition to be administered to at least one mucous membrane selected from the group consisting of a human or animal intraoral mucous membrane, ocular mucous membrane, ear mucous membrane, genital mucous membrane, pharyngeal mucous membrane, respiratory tract mucous membrane, bronchial mucous membrane, pulmonary mucous membrane, gastric mucous membrane, enteric mucous membrane, and rectal mucous membrane, containing: at least one antigen; and as an adjuvant, a lipopolysaccharide derived from at least one gram-negative bacterium selected from the group consisting of Serratia, Leclercia, Rahnella, Acidicaldus, Acidiphilium, Acidisphaera, Acidocella, Acidomonas, Asaia, Belnapia, Craurococcus, Gluconacetobacter, Gluconobacter, Kozakia, Leahibacter, Muricoccus, Neoasaia, Oleomonas, Paracraurococcus, Rhodopila, Roseococcus, Rubritepida, Saccharibacter, Stella, Swaminathania, Teichococcus, Zavarzinia, Pseudomonas, Achromobacter, Bacillus, Methanoculleus, Methanosarcina, Clostridium, Micrococcus, Flavobacterium, Pantoea, Acetobacter, Zymomonas, Xanthomonas, and Enterobacter, or a salt thereof.

The invention provides an inoculant comprising Pseudomonas diazotrophicus and a carrier, which is useful for enhancing plant growth. The invention also provides a method of producing a plant which comprises applying an inoculant comprising Pseudomonas diazotrophicus and a carrier to plant or the growth medium of the plant.

A composition comprising a purified extract isolated from Pseudolysimachion rotundum var. subintegrum containing abundant amount of active ingredient or the compounds isolated therefrom as an active ingredient for treating a chronic obstructive pulmonary disease and the use thereof. Inventive purified extract and compounds showed potent anti-COPD activity without beta-2-receptor agonistic response through various in vivo tests as well as in vitro test. Therefore, it can be used as the therapeutics or functional health food for treating and preventing chronic obstructive pulmonary disease (COPD).

The invention provides a composition containing a mixture of a first phenyl-carbamoyl thiazole derivative and a second phenyl-carbamoyl thiazole derivative which are different from each other. Together with extracts of plants such as crotalaria assamica, star anise fruits, calophyllum membranaceum, schisandrae virides caulis, cycas leaves and the like, the mixture can generate synergistic antibacterial action on staphylococcus hominis, pantoea agglomerans, kocuria kristinae, serratia liquefaciens, ralstonia pickettii, peptostreptococcus anaerobius, klebsiella oxytoca, corynebacterium macginleyi, listeria monocytogens, simulation corynebacterium, clostridium perfringens, staphylococcus haemolyticus, aeromonas hydrophila, acinetobacter baumannii, streptococcus sanguis, peptostreptococcus prevotii, propionibacterium acnes, pseudomonas oryzihabitans, staphylococcus warneri, and legionella pneumophila.

The invention discloses a preparation method of an enzyme washing liquid for automobiles. The technical scheme includes that the enzyme washing liquid is prepared by mixing stephania tetrandra root extract, dipotassium glycyrrhizinate, natto fermented extract, oat polypeptide, polyquaternium, bisabolol, thiamine hydrochloride, phytase, rhodopseudomonas palustris, probiotics, methylethyl ester, capryloyl salicylic acid, AES, trehalose, kathon 2, sodium alcohol ether sulfate, enzymes, acetic acid, lemon flavor and water.

The invention relates to a kitchen garbage degrading composite microbial inoculum and a preparation method and application thereof. The kitchen garbage degrading composite microbial inoculum is prepared by mixing a mixed inoculum of bacillus amyloliquefaciens, radiation-resistant methylobacterium, dispersed pantoea, pseudomonas oryzihabitans, citrobacter freundii and staphylococcus cohnii and a carrier, wherein the mass of the mixed inoculum accounts for 15-25% of the total mass of the kitchen garbage-degrading composite microbial inoculum; the mass ratio of the bacillus amyloliquefaciens, the radiation-resistant methylobacterium, the dispersed pantoea, the pseudomonas oryzihabitans, the citrobacter freundii and the staphylococcus cohnii is (1.5-3):(1-1.5):(1-1.5): (1-1.5):(1-1.5):(1-1.5); the kitchen garbage-degrading composite microbial inoculum is used for degrading kitchen garbage. Compared with the prior art, the kitchen garbage degrading composite microbial inoculum has the advantages as follows: the kitchen garbage degrading composite microbial inoculum can effectively degrade common vegetables, grains, fish, poultry meat and other kitchen garbage, has the degradation rate being above 80%, is high in degradation speed rate and small in odor, does not produce pollutants or poisonous substances, and is low in cost and stable in performance.

The invention discloses a free living nitrogen fixing bacterium pseudomonas oryzihabitans MBC4. The preservation number of the free living nitrogen fixing bacterium pseudomonas oryzihabitans MBC4 is CGMCC No.10820, the preservation date is May 19, 2015, and the preservation institution is China General Microbiological Culture collection Center. The strain can still live, breed and have nitrogen fixation activity under drought, large temperature differences, salt and alkali disasters and strong ultraviolet radiation. A microbial agent prepared from the free living nitrogen fixing bacterium pseudomonas oryzihabitans MBC4 can be applied to ecological systems with drought, large temperature differences, salt and alkali disasters and strong ultraviolet radiation, can increase nitrogen accumulation in the ecological systems and can perform the function of improving soil environments and the physiological activity of microorganisms and plants and increasing net primary productivity.