37 results about "Pseudomonas oryzihabitans" patented technology

The invention relates to an application of pseudomonas chlororaphis subsp.aureofaciens in preventing and controlling nematode. The pseudomonas chlororaphis subsp.aureofaciens is chlororaphis subsp.aureofaciens GDMCC NO. 60273. The invention relates to the application of pseudomonas chlororaphis subsp.aureofaciens in preparing a biological bacteriostatic agent for preventing and controlling nematode. The biological bacteriostatic agent includes the chlororaphis subsp.aureofaciens served as an active ingredient and a carrier. The biological bacteriostatic agent is chlororaphis subsp.aureofaciensfermentation liquor. A method for preventing and controlling nematode comprises the step of spraying the biological bacteriostatic agent containing chlororaphis subsp.aureofaciens in claim 1 and / or fermentation liquor thereof onto an affected part. According to the application of pseudomonas chlororaphis subsp.aureofaciens in preventing and controlling nematode provided by the invention, chlororaphis subsp.aureofaciens used for preventing and controlling nematode is free from pesticide residue, free from environmental pollution and safe to human and livestock. Application of pseudomonas chlororaphis subsp.aureofaciens in preparing the biological bacteriostatic agent for preventing and controlling nematode is characterized by simple preparation process, low cost, excellent effect and convenience in use.

The invention discloses a molecular beacon probe for rapidly detecting urinary tract infection bacteria. The molecular beacon probe is Beacon NL. The nucleotide sequence of the probe is Beacon NL:5'-CY3-TTCCACACAAGCGGTGGAGCATGTGGTTTGGAA-BHQ1- 3'; the 5' terminal of the probe is marked with CY3, the 3' terminal of the probe is marked with BHQ1, the excitation wavelength of a fluorescent group is 552 nm, and the detection wavelength is 570nm. The molecular beacon probe uses16s rRNA of the bacteria as target spots and can detect six kinds of urinary tract infection bacteria, namely escherichia coli, enterococcus, pseudomonas aeruginosa, proteus mirabilis, klebsiella pneumoniae and staphylococcus. The probe is high in fluorescence intensity and specificity. The invention further discloses a kit and method for rapidly detecting the urinary tract infection bacteria.

The invention relates to a compound microbial agent for relieving continuous cropping obstacles of melon crops and application. The compound microbial agent is composed of pseudomonas oryzihabitans and trichoderma according to a ratio of 1:3-3:1, the concentration of the pseudomonas oryzihabitans in bacterial liquid is 1.0-3.0x107cfu / ml, and the concentration of trichoderma in the bacterial liquidis 1.0-3.0x107cfu / mL. The application method of the composite microbial agent comprises the steps that an application method of hole application or root irrigation is adopted in the transplanting period of melon crops or in the flowering and fruit setting period of the melon crops, and the dosage of the microbial agent for each seedling is 250ml. The composite microbial agent is a mixed bacteriaof melon plant rhizosphere soil separating bacteria and trichoderma probiotics, has good colonizing ability in the rhizosphere of the melon crops, is safe and reliable, has a good degradation effect on autotoxins in soil of continuous cropping melon crops, obviously improves the physical and chemical properties of the soil, and has an obvious promotion effect on the stress resistance of the meloncrops.

Methylotrophic or methanotrophic bacteria such as Methylobacterium are transformed with a gene of interest, and expression of the gene is regulated by means of a cumate repressor protein and an operator sequence which is operatively linked to the gene of interest, and the addition of an external agent. Specifically, the cymR repressor and cmt operator from Pseudomonas putida may serve to regulate gene expression in methylotrophic or methanotrophic bacteria with the addition of cumate.

The invention discloses a strain of pseudomonas producing ferulic acid esterase and application thereof, belonging to the fields of fermentation engineering and biotechnology. The Pseudomonas oryzihabitans Y‑5 of the present invention was deposited in the China Center for Type Culture Collection on July 13, 2018, with the preservation number CCTCC NO: M 2018480. The strain can secrete ferulic acid esterase. Under the induction of bran, the ferulic acid esterase activity of the fermented crude enzyme solution is 116U / L, and ferulic acid is produced by fermentation with wheat bran as the substrate, and the yield can reach 59.51±0.6mg / L. The Pseudomonas of the present invention can increase the content of ferulic acid in the rice wine without affecting the alcohol content, pH value and reducing sugar content of the rice wine. The content of ferulic acid is about 2.6 times that of traditional wheat koji.

The invention provides a frozen microorganism soil mixing pile. Soft soil is forcibly mixed with a water reducing agent, a bacterial liquid and a cementing solution on site in a foundation, so that the soft soil is hardened into foundation soil; and then a freezing method is implemented, so that the phenomena of frost heaving and thaw collapse of surrounding stratums can be effectively inhibited, and the bearing capacity, the shear strength, the pile body strength and the impermeability of the microorganism soil mixing pile are enhanced. According to the frozen microorganism soil mixing pile, soft soil gaps are filled with the cementing solution, and the bacterial liquid containing bacillus pasteurii and pseudomonas oryzihabitans is added, so that cracks generated when the mixing pile is frozen are repaired, and the bearing capacity of the mixing pile is improved; and by adding the water reducing agent, the impermeability of the microorganism soil mixing pile is improved, and the influence of water on the freezing effect is reduced.

The present invention aims at providing a mucosal vaccine composition that can be administered to an intraoral mucous membrane, ocular mucous membrane, ear mucous membrane, genital mucous membrane, pharyngeal mucous membrane, respiratory tract mucous membrane, bronchial mucous membrane, pulmonary mucous membrane, gastric mucous membrane, enteric mucous membrane, or rectal mucous membrane, that is useful as a prophylactic or therapeutic agent for infectious diseases or cancers, and is capable of safely and effectively inducing the systemic immune response and mucosal immune response. The present invention provides a mucosal vaccine composition to be administered to at least one mucous membrane selected from the group consisting of a human or animal intraoral mucous membrane, ocular mucous membrane, ear mucous membrane, genital mucous membrane, pharyngeal mucous membrane, respiratory tract mucous membrane, bronchial mucous membrane, pulmonary mucous membrane, gastric mucous membrane, enteric mucous membrane, and rectal mucous membrane, containing: at least one antigen; and as an adjuvant, a lipopolysaccharide derived from at least one gram-negative bacterium selected from the group consisting of Serratia, Leclercia, Rahnella, Acidicaldus, Acidiphilium, Acidisphaera, Acidocella, Acidomonas, Asaia, Belnapia, Craurococcus, Gluconacetobacter, Gluconobacter, Kozakia, Leahibacter, Muricoccus, Neoasaia, Oleomonas, Paracraurococcus, Rhodopila, Roseococcus, Rubritepida, Saccharibacter, Stella, Swaminathania, Teichococcus, Zavarzinia, Pseudomonas, Achromobacter, Bacillus, Methanoculleus, Methanosarcina, Clostridium, Micrococcus, Flavobacterium, Pantoea, Acetobacter, Zymomonas, Xanthomonas, and Enterobacter, or a salt thereof.

A composition comprising a purified extract isolated from Pseudolysimachion rotundum var. subintegrum containing abundant amount of active ingredient or the compounds isolated therefrom as an active ingredient for treating a chronic obstructive pulmonary disease and the use thereof. Inventive purified extract and compounds showed potent anti-COPD activity without beta-2-receptor agonistic response through various in vivo tests as well as in vitro test. Therefore, it can be used as the therapeutics or functional health food for treating and preventing chronic obstructive pulmonary disease (COPD).

The invention provides a composition containing a mixture of a first phenyl-carbamoyl thiazole derivative and a second phenyl-carbamoyl thiazole derivative which are different from each other. Together with extracts of plants such as crotalaria assamica, star anise fruits, calophyllum membranaceum, schisandrae virides caulis, cycas leaves and the like, the mixture can generate synergistic antibacterial action on staphylococcus hominis, pantoea agglomerans, kocuria kristinae, serratia liquefaciens, ralstonia pickettii, peptostreptococcus anaerobius, klebsiella oxytoca, corynebacterium macginleyi, listeria monocytogens, simulation corynebacterium, clostridium perfringens, staphylococcus haemolyticus, aeromonas hydrophila, acinetobacter baumannii, streptococcus sanguis, peptostreptococcus prevotii, propionibacterium acnes, pseudomonas oryzihabitans, staphylococcus warneri, and legionella pneumophila.

The invention relates to a kitchen garbage degrading composite microbial inoculum and a preparation method and application thereof. The kitchen garbage degrading composite microbial inoculum is prepared by mixing a mixed inoculum of bacillus amyloliquefaciens, radiation-resistant methylobacterium, dispersed pantoea, pseudomonas oryzihabitans, citrobacter freundii and staphylococcus cohnii and a carrier, wherein the mass of the mixed inoculum accounts for 15-25% of the total mass of the kitchen garbage-degrading composite microbial inoculum; the mass ratio of the bacillus amyloliquefaciens, the radiation-resistant methylobacterium, the dispersed pantoea, the pseudomonas oryzihabitans, the citrobacter freundii and the staphylococcus cohnii is (1.5-3):(1-1.5):(1-1.5): (1-1.5):(1-1.5):(1-1.5); the kitchen garbage-degrading composite microbial inoculum is used for degrading kitchen garbage. Compared with the prior art, the kitchen garbage degrading composite microbial inoculum has the advantages as follows: the kitchen garbage degrading composite microbial inoculum can effectively degrade common vegetables, grains, fish, poultry meat and other kitchen garbage, has the degradation rate being above 80%, is high in degradation speed rate and small in odor, does not produce pollutants or poisonous substances, and is low in cost and stable in performance.

The invention discloses a free living nitrogen fixing bacterium pseudomonas oryzihabitans MBC4. The preservation number of the free living nitrogen fixing bacterium pseudomonas oryzihabitans MBC4 is CGMCC No.10820, the preservation date is May 19, 2015, and the preservation institution is China General Microbiological Culture collection Center. The strain can still live, breed and have nitrogen fixation activity under drought, large temperature differences, salt and alkali disasters and strong ultraviolet radiation. A microbial agent prepared from the free living nitrogen fixing bacterium pseudomonas oryzihabitans MBC4 can be applied to ecological systems with drought, large temperature differences, salt and alkali disasters and strong ultraviolet radiation, can increase nitrogen accumulation in the ecological systems and can perform the function of improving soil environments and the physiological activity of microorganisms and plants and increasing net primary productivity.