Molecular beacon probe, kit and method for rapidly detecting urinary tract infection bacteria

Abstract

The invention discloses a molecular beacon probe for rapidly detecting urinary tract infection bacteria. The molecular beacon probe is Beacon NL. The nucleotide sequence of the probe is Beacon NL:5'-CY3-TTCCACACAAGCGGTGGAGCATGTGGTTTGGAA-BHQ1- 3'; the 5' terminal of the probe is marked with CY3, the 3' terminal of the probe is marked with BHQ1, the excitation wavelength of a fluorescent group is 552 nm, and the detection wavelength is 570nm. The molecular beacon probe uses16s rRNA of the bacteria as target spots and can detect six kinds of urinary tract infection bacteria, namely escherichia coli, enterococcus, pseudomonas aeruginosa, proteus mirabilis, klebsiella pneumoniae and staphylococcus. The probe is high in fluorescence intensity and specificity. The invention further discloses a kit and method for rapidly detecting the urinary tract infection bacteria.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a molecular beacon probe and a kit for detecting urinary tract infection bacteria in a sample by means of fluorescence in situ hybridization. Background technique [0002] More than 95% of UTIs are caused by a single bacterium. In about 50% of the patients, the pathogenic bacteria is Escherichia coli, which can be serotyped up to 140 species. It is mostly seen in asymptomatic bacteriuria or uncomplicated urinary infection; besides Escherichia coli, Enterococcus, Pseudomonas aeruginosa, Proteus mirabilis, Klebsiella and other bacteria are common in reinfection, indwelling catheter , Urinary tract infection with complications; Staphylococcus is more common in skin wounds and drug addicts. A variety of bacterial co-infections are seen in indwelling catheters, neurogenic bladder, stones, congenital malformations, and vaginal, intestinal, and urethral fistulas. [0003]...

AI Technical Summary

Problems solved by technology

Among them, the bacterial culture method is the gold standard for detecting bacteria in urinary tract infections, but this method requires drug susceptibility testing, usually takes 3-5 days to see the final test results, and the detection cost is high and the detection period is long; the PCR method can be used in In a short period of time, the copy number of a specific nucleic acid sequence increases geometrically, which has high sensitivity and specificity, but the disadvantage is that the false positive rate is high. On the other hand, the urine of patients often contains components that inhibit the PCR reaction. It will lead to the occurrence of false negatives, and the shortcomings of these two aspects limit the clinical application of PCR detection.

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