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Biological microbial agent for preventing and treating strawberry root rot bacteria

A technology for strawberry root rot and microbial inoculants, applied in the directions of chemicals for biological control, biocides, microorganism-based methods, etc., can solve drug resistance and strawberry quality decline, damage to seedlings, and economic benefits problems, to achieve the effect of improving soil physical and chemical properties, good degradation, and reasonable formula

Inactive Publication Date: 2018-10-12
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For strawberry farmers, during the 3-4 years of strawberry picking, frequent crop rotation will lead to lower economic benefits, and the method of fertilization will easily cause adverse consequences such as injury to seedlings, and single chemical control will cause drug resistance and strawberry quality decline

Method used

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Examples

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Embodiment 1

[0016] This example provides a biological fungal agent that can effectively prevent and treat strawberry root rot, while alleviating the autotoxicity of strawberry and promoting growth and development.

[0017] 1 Materials and methods

[0018] 1.1 Test materials

[0019] Strawberry varieties tested: Dandong 99

[0020] The medium used to isolate Pseudomonas oryzae is an inorganic salt medium, and the formula includes MgSO 4 ·7H 2 O 0.2 g / L, CaCl 2 2H 2 O0.02g / L, FeCl 3 0.05g / L, KH 2 PO 4 1.0 g / L, K 2 HPO 4 1.0 g / L, NH 4 NO 3 1.0 g / L, cholesterol 0.3 g / L, and agar 17 g / L, the treatment concentration was 200 μg·mL -1 The p-hydroxybenzoic acid, benzoic acid and cinnamic acid are used as the sole carbon source, and the pH value is adjusted to neutral.

[0021] The method for isolating Pseudomonas oryzae: wash the junction of muskmelon rhizome with running water and cut it into small pieces, soak in 75% alcohol for 1 min, rinse with 0.1% mercury liter for 30 s, rin...

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PUM

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Abstract

The invention relates to a microbial agent for preventing and treating strawberry root rot. The microbial agent comprises a Pseudomonas oryzihabitans bacterial solution and a synergist, wherein Pseudomonas oryzihabitans is collected by the General Microorganisms Center of China Committee for Culture Collection of Microorganisms, which is located at Institute of Microbiology, Chinese Academy of Sciences, 3#, 1# Courtyard, Beichen West Road, Chaoyang District, Beijing, on January 29, 2018 and has the collection number of CGMCC No. 15325; and the synergist is a 4,000-5,000-solution mixed solutionof chlorothalonil and Hymexazol. The bacterial fertilizer has good colonization capability at rhizosphere of strawberries, is safe and reliable and plays a good role in degrading root rot pathogenicbacteria in continuous cropping strawberry soil, so that the physicochemical properties of the soil are obviously improved, the stress resistance of strawberry plants is promoted, and the yield and fruit quality of strawberries are improved.

Description

technical field [0001] The invention relates to the field of biological control, in particular to a biological bacterial agent for controlling strawberry root rot. Background technique [0002] Strawberry root rot mainly damages the root system. When the onset starts from small lateral roots or new roots, irregular light red and brown plaques appear at first, and the color gradually darkens to dark brown. As the disease develops, all roots rapidly become necrotic and brown. The aboveground part is that the outer leaf margins turn yellow, brown, necrotic, and shrink. The diseased plant shows a lack of water, and gradually develops towards the center until the whole plant is yellow and dead. The root system of strawberry root rot is shorter and darker than that of healthy plants, and the adventitious roots in the underground part die in large numbers, and the new roots are attacked by pathogenic bacteria and grow sparsely. The roots are damaged, the absorption capacity decre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01N37/34A01N43/80A01P3/00A01P21/00C12N1/20C12R1/38
CPCA01N63/00A01N37/34A01N43/80C12N1/20
Inventor 孟祥东
Owner SHENYANG AGRI UNIV
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