Biological microbial agent for preventing and treating strawberry root rot bacteria
A technology for strawberry root rot and microbial inoculants, applied in the directions of chemicals for biological control, biocides, microorganism-based methods, etc., can solve drug resistance and strawberry quality decline, damage to seedlings, and economic benefits problems, to achieve the effect of improving soil physical and chemical properties, good degradation, and reasonable formula
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[0016] This example provides a biological fungal agent that can effectively prevent and treat strawberry root rot, while alleviating the autotoxicity of strawberry and promoting growth and development.
[0017] 1 Materials and methods
[0018] 1.1 Test materials
[0019] Strawberry varieties tested: Dandong 99
[0020] The medium used to isolate Pseudomonas oryzae is an inorganic salt medium, and the formula includes MgSO 4 ·7H 2 O 0.2 g / L, CaCl 2 2H 2 O0.02g / L, FeCl 3 0.05g / L, KH 2 PO 4 1.0 g / L, K 2 HPO 4 1.0 g / L, NH 4 NO 3 1.0 g / L, cholesterol 0.3 g / L, and agar 17 g / L, the treatment concentration was 200 μg·mL -1 The p-hydroxybenzoic acid, benzoic acid and cinnamic acid are used as the sole carbon source, and the pH value is adjusted to neutral.
[0021] The method for isolating Pseudomonas oryzae: wash the junction of muskmelon rhizome with running water and cut it into small pieces, soak in 75% alcohol for 1 min, rinse with 0.1% mercury liter for 30 s, rin...
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