Post-neurosurgical intracranial bacterial infection common pathogenic bacterium MLPA detection probe and application thereof

Abstract

The invention discloses a post-neurosurgical intracranial bacterial infection common pathogenic bacterium MLPA detection probe and an application thereof. The probe comprises a first group of probes to a seventeenth group of probes, and the first group of probes to the seventeenth group of probes are probes for detecting Neisseria meningitides, Bacillus pyocyaneus, Salmonella, Stenotrophomonas maltophilia, Staphylococcus aureus, Staphylococcus epidermidis, Klebsiella pneumonia, Serratia marcescens, Acinetobacter lwoffii, Corynebacteria diphtheria, Streptococcus mitis, Acinetobacter baumannii, Streptococcus pneumonia, Propionibacterium acnes, Listeria monocytogenes, Enterobacter cloacae and Haemophilus influenza sequentially. The probe is developed through an MLPA technology, can rapidly diagnose post-neurosurgical intracranial bacterial infection 17 common pathogenic bacteria once, provides possibility for rapid diagnosis of pathogenic bacteria of like patients, and is helpful for reducing the fatality and the disability rate of like infection.

Description

technical field [0001] The invention belongs to the technical field of pathogenic microorganism detection, and in particular relates to a common pathogenic bacteria MLPA detection probe for intracranial bacterial infection after neurosurgery and an application thereof. Background technique [0002] Intracranial bacterial infection following neurosurgery most commonly manifests as meningitis, subdural empyema, or brain abscess. The mortality rate of intracranial infection reported abroad is 27.4% to 35% [42,43]. Domestic scholars reported that the mortality rate of intracranial bacterial infection after severe neurosurgery can be as high as 57% [44]. [0003] There are dozens of pathogenic bacteria in intracranial bacterial infection after neurosurgery, and nearly 20 common species come from nearly 10 different bacterial genera. Most of them are Gram-positive bacteria, the most common pathogens are Staphylococcus aureus and Staphylococcus epidermidis (31% and 37%, respectiv...

AI Technical Summary

Problems solved by technology

However, the detection throughput of this technology is low, and it can only detect up to 8 kinds of pathogenic bacteria at the same time, and the missed diagnosis rate is high.

In theory, although microarray chips and sequencing technologies can realize the simultaneous detection of most pathogenic bacteria, these technologies involve complex analysis of large amounts of data and require personnel with specialized skills

In short, at present, there is no clinical kit for one-time diagnosis of more than 8 kinds of pathogenic bacteria in intracranial bacterial infection after neurosurgery craniotomy

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