Post-neurosurgical intracranial bacterial infection common pathogenic bacterium MLPA detection probe and application thereof

A neurosurgery and detection probe technology, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, and resistance to vector-borne diseases, etc., can solve the problems of high missed diagnosis rate, complex data analysis, low detection throughput, etc., and achieve reduction Effects on fatality and morbidity

Inactive Publication Date: 2015-11-04
HUAQIAO UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the detection throughput of this technology is low, and it can only detect up to 8 kinds of pathogenic bacteria at the same time, and the missed diagnosis rate is high.
In theory, although microarray chips and sequencing technologies can realize the simultaneous detection of most pathogenic b

Method used

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  • Post-neurosurgical intracranial bacterial infection common pathogenic bacterium MLPA detection probe and application thereof
  • Post-neurosurgical intracranial bacterial infection common pathogenic bacterium MLPA detection probe and application thereof
  • Post-neurosurgical intracranial bacterial infection common pathogenic bacterium MLPA detection probe and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 Detection target design of pathogenic bacteria in intracranial infection

[0054] First, the common pathogenic bacteria of intracranial bacterial infection after neurosurgery are summarized, as shown in Table 1:

[0055] Table 1. Summary of common pathogens showing intracranial bacterial infection after neurosurgery

[0056]

[0057]

[0058] In principle, the process of determining the detection target of pathogenic bacteria in intracranial infection: enter PubMed to find the conserved sequence specific to each pathogen, such as the sequence of housekeeping gene 16S rRNA, rpoB, gyrB or ITS, as shown in Table 2 Shown:

[0059] Table 2. Detection targets of common pathogenic bacteria in intracranial bacterial infection after neurosurgery

[0060]

Embodiment 2

[0061] Example 2 The reference sequence of the MLPA probe hybridization target

[0062] After analyzing and comparing the conserved sequences of 17 pathogenic bacteria in neurosurgery intracranial infection by Blast tool, the reference sequence was determined to design hybridization probes, as shown in Table 3:

[0063] Table 3. Reference Sequences Showing Hybridization Targets of MLPA Probes

[0064]

Embodiment 3

[0065] Example 3 left probe LPO

[0066] The sequence of the left probe LPO for MLPA analysis of intracranial infection of 17 kinds of pathogenic bacteria after neurosurgery designed by the present invention is shown in Table 4:

[0067] Table 4. Design results of the left probe LPO sequence (for the upstream universal primer + hybridization sequence)

[0068]

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Abstract

The invention discloses a post-neurosurgical intracranial bacterial infection common pathogenic bacterium MLPA detection probe and an application thereof. The probe comprises a first group of probes to a seventeenth group of probes, and the first group of probes to the seventeenth group of probes are probes for detecting Neisseria meningitides, Bacillus pyocyaneus, Salmonella, Stenotrophomonas maltophilia, Staphylococcus aureus, Staphylococcus epidermidis, Klebsiella pneumonia, Serratia marcescens, Acinetobacter lwoffii, Corynebacteria diphtheria, Streptococcus mitis, Acinetobacter baumannii, Streptococcus pneumonia, Propionibacterium acnes, Listeria monocytogenes, Enterobacter cloacae and Haemophilus influenza sequentially. The probe is developed through an MLPA technology, can rapidly diagnose post-neurosurgical intracranial bacterial infection 17 common pathogenic bacteria once, provides possibility for rapid diagnosis of pathogenic bacteria of like patients, and is helpful for reducing the fatality and the disability rate of like infection.

Description

technical field [0001] The invention belongs to the technical field of pathogenic microorganism detection, and in particular relates to a common pathogenic bacteria MLPA detection probe for intracranial bacterial infection after neurosurgery and an application thereof. Background technique [0002] Intracranial bacterial infection following neurosurgery most commonly manifests as meningitis, subdural empyema, or brain abscess. The mortality rate of intracranial infection reported abroad is 27.4% to 35% [42,43]. Domestic scholars reported that the mortality rate of intracranial bacterial infection after severe neurosurgery can be as high as 57% [44]. [0003] There are dozens of pathogenic bacteria in intracranial bacterial infection after neurosurgery, and nearly 20 common species come from nearly 10 different bacterial genera. Most of them are Gram-positive bacteria, the most common pathogens are Staphylococcus aureus and Staphylococcus epidermidis (31% and 37%, respectiv...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/14C12Q1/04C12N15/11
CPCC12Q1/6816C12Q2525/131Y02A50/30
Inventor 王明席李云振王庆波姜涛
Owner HUAQIAO UNIVERSITY
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