18 results about "Acid Esterase" patented technology

The invention relates to a high-temperature-resistant feruloyl esterase liquid as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) constructing a reaction system: suspending recombinant microorganism thalli for expressing high-temperature-resistant feruloyl esterase in a buffer solution with the pH value of 7.0-9.0; (2) ultrasonic cracking and thermal purification: heating the reaction system to 70-85 DEG C under the condition of ultrasonic crushing, and keeping the temperature for 1-8 hours; carrying out structural destruction on the microbial thalli by utilizing ultrasound so as to release intracellular high-temperature-resistant feruloyl esterase, and carrying out denaturation and precipitation on host impure protein by utilizing the thermal stability difference between the high-temperature-resistant feruloyl esterase and the host impure protein; after the high-temperature-resistant feruloyl esterase is subjected to heat treatment in the step (2), the catalytic activity is kept at the temperature of 60 DEG C or above; and (3) obtaining an enzyme solution, namely centrifuging the reaction body fluid subjected to the ultrasonic treatment and the heat treatment in the step (2), and collecting supernate, so as to obtain the high-temperature-resistant feruloyl esterase solution.

The invention belongs to the technical field of microorganisms, and provides a high-yield feruloyl esterase plant lactobacillus and application thereof. The bacterial strain is lactobacillus plantarum Fs6, the preservation number of the bacterial strain is CGMCC (China General Microbiological Culture Collection Center) No.36264, and the preservation date of the bacterial strain is October 20, 2025. According to the present invention, the fermentation performance of the dough can be significantly improved with the plant lactobacillus, and the good synergistic effect is represented during the co-fermentation with the yeast; and a good synergistic effect is shown during co-fermentation with yeast. When the mass concentration of bile salt is 0.3 and 0.6 g / L, the survival rate is relatively good, which shows that the lactic acid bacteria Fs6 has a certain probiotic function. The self-aggregation force (32.77%) and the hydrophobicity (32.43%) of the Fs6 are relatively low, and the adhesion of the Fs6 is relatively poor. And combined phenolic acid in the grains can be effectively released, so that the nutritional value of the grains can play a better health role in a human body.

Provided are the screening and identification of a Pseudomonas sp. strain for producing feruloyl esterase at high yield and a use of the Pseudomonas sp. strain as a bioaugmentation agent in fermented foods, relating to the technical field of brewing. By means of screening, a Pseudomonas sp. strain A-4H for producing feruloyl esterase at high yield is obtained. After cultivation for 36 h, the Pseudomonas sp. strain A-4H can produce ferulic acid at a concentration of 153.79±2.36 mg / L, and the activity of feruloyl esterase in a fermentation broth reaches 136.63±10.27 U / L. The addition of the Pseudomonas sp. A-4H during the brewing of Huangjiu can effectively increase the content of ferulic acid, the content of phenolic acid, and the content of organic acid in Huangjiu, providing an effective biological pathway for increasing the content of ferulic acid in fermented foods.

The application discloses a carboxylic esterase lyophilization protective agent, a preparation method and application thereof, and belongs to the technical field of bioengineering. The carboxylic esterase lyophilization protective agent is a single-component polyvinylpyrrolidone; wherein, the polyvinylpyrrolidone has a viscosity-average molecular weight Mv of 10000-15000000; a K value of 17-90; when used for lyophilization protection of carboxylic esterase, the carboxylic esterase lyophilized powder can effectively improve the stability of the carboxylic esterase lyophilized powder at normal temperature, prevent the purity and activity of the carboxylic esterase lyophilized powder from decreasing, and the lyophilized powder cake is complete, easy to be powdered, the enzyme powder resuspension is clear and transparent, the enzyme activity recovery rate is high, and the carboxylic esterase is convenient for being applied to different application scenarios.

The invention provides a high temperature resistant feruloyl esterase and an application thereof. The feruloyl esterase wild type has a remarkable high-temperature-resistant characteristic and still shows relatively high thermal stability under the condition of 95 DEG C, and the thermal inactivation half-life period of the feruloyl esterase wild type can reach 5h. On the basis, after the feruloyl esterase mutant obtained through structural rationality modification continuously reacts for 5 h at the temperature of 75 DEG C, floccule precipitation is not observed, and stable catalytic activity is kept. In the application, the feruloyl esterase mutant disclosed by the invention and commercial xylanase have a synergistic effect, so that the release of bound ferulic acid in starch-removed wheat bran can be effectively promoted. The invention provides a novel enzymology tool for efficiently and quickly releasing the plant-derived ferulic acid under a high-temperature condition.

This invention discloses a near-infrared fluorescent probe for detecting bone regeneration vitality and its preparation method, belonging to the field of biochemistry. Using rhodamine as the fluorescent core, the invention utilizes quinoline ring expansion to increase the conjugated area and extend emission. The brightness and stability of the probe are controlled by adjusting the steric hindrance of the aromatic ring at position 9, constructing a series of stable, high-brightness probe cores with low pKa. By introducing a phosphate ester group as a responder while simultaneously quenching the fluorescence of the core, a near-infrared fluorescent probe, SiRd-MOP, for detecting bone regeneration vitality is obtained. The preparation method of this invention has high yield and mild reaction conditions. The prepared probe can specifically and sensitively respond to alkaline phosphatase (ALP), and has successfully achieved ALP activity monitoring in osteogenic differentiation models and throughout the entire process of fracture regeneration and repair in mice. It can be used for real-time assessment of bone regeneration vitality and has very high biological application prospects.

The invention provides a biosynthesis method of an esdoxaban tosylate chiral intermediate. According to the preparation method, 3-cyclohexene-1-carboxylic acid methyl ester is taken as a starting raw material, and a target intermediate is finally obtained through carboxylesterase resolution, hydrolysis, hydroxylation, dehydrogenation oxidation, transamination reaction, amidation and Boc2O reaction in sequence. According to the method, high-selectivity carboxylesterase is used for realizing chiral resolution; p450 oxidase with excellent regioselectivity and stereoselectivity is utilized to realize specific hydroxylation of a C-H bond under a mild condition, and a traditional process depending on a highly toxic chemical oxidant is overturned; then, clean oxidation is realized through alcohol dehydrogenase, and a way is laid for subsequent reaction; most importantly, the transaminase is adopted to directly construct the chiral amine, so that the use of a high-risk reagent sodium azide is thoroughly avoided, and major potential safety hazards are eliminated from the source; and finally, green amidation is realized by virtue of lipase, and an acyl chloride method for generating corrosive wastewater is replaced.

The present invention provides compositions used for the treatment and / or prevention of protein aggregation disorders. [Solution] Proteopathy encompasses a wide range of ailments, including neurodegenerative diseases (e.g., polyglutamine diseases such as huntingtin in Alzheimer's disease, Parkinson's disease, and Huntington's disease, and prion diseases); amyloidosis of other non-neuronal proteins (especially I1-antitrypsin, immunoglobulin light and heavy chains, lactadherin, apolipoprotein, gelzolin, lysozyme, fibrinogen, atrial natriuretic factor, keratin, lactoferrin, and β-2 microglobulin, etc.); sickle cell disease; cataracts; cystic fibrosis; retinitis pigmentosa; and nephrogenic diabetes insipidus. Administration of sulfatase inhibitors is generally suitable for treating and / or preventing protein toxicity associated with proteopathy. Therefore, the present invention provides compositions comprising sulfatase inhibitors for the treatment of proteopathy.

The invention relates to the technical field of microbial application and bioengineering, and discloses an aspergillus awamori SNJ-2F capable of simultaneously producing feruloyl esterase, cellulase and xylanase at high yield, which is separated from Shennongjia high-temperature yeast for making hard liquor and preserved in China Center for Type Culture Collection with the preservation number of CCTCC NO: M20252024. The invention also discloses a screening method of the strain, a bran seed preparation process and a method for producing ferulic acid by performing enzymolysis on starch-removed wheat bran with a bran seed crude enzyme. The strain disclosed by the invention is strong in enzyme system synergy, green in process and low in cost, can be used for ferulic acid preparation, white spirit / soy sauce brewing and feed industry, and has a wide application prospect.

The application discloses a recombinant feruloyl esterase and a mutant and application thereof, and belongs to the technical field of enzyme engineering. Clostridium butyricum, The wild-type enzyme amino acid sequence is shown as SEQ ID NO:1, and the coding gene sequence is shown as SEQ ID NO:2; the target enzyme is obtained by constructing a recombinant expression vector containing the CbFAE gene, inducing expression in Escherichia coli BL21 (DE3) and purifying; substrate spectrum analysis shows that the enzyme not only has catalytic decomposition activity of model substrates such as methyl ferulate, but also has catalytic activity of decomposing mono (2-hydroxyethyl) terephthalate (MHET) into terephthalic acid (TPA) and ethylene glycol (EG); the application also constructs the CbFAE mutant L168R based on sequence and structure analysis, the amino acid sequence of the mutant enzyme is shown as SEQ ID NO:3, and the catalytic MHET decomposition activity of the mutant enzyme is increased by nearly 4 times compared with the wild type under the same reaction condition. The recombinant feruloyl esterase and the mutant provided by the application expand the types of MHET hydrolytic enzymes and provide a new way for the biodegradation of MHET.

The invention provides a DNA molecule for coding feruloyl esterase, a recombinant expression vector, a genetically engineered bacterium and application of the genetically engineered bacterium in production of feruloyl esterase, and belongs to the technical field of microbial genetic engineering. The nucleotide sequence of the DNA molecule for coding the feruloyl esterase is as shown in SEQ ID No. 1, and the amino acid sequence correspondingly coded by the DNA molecule is as shown in SEQ ID No. 2. The invention also discloses a recombinant expression vector and a genetically engineered bacterium which comprise the DNA molecule for coding the feruloyl esterase. According to the feruloyl esterase BccFae3074, a strain extracted from soil is used for sequencing, then a corresponding expression vector and engineering bacteria are obtained through genetic engineering modification, and the produced feruloyl esterase has relatively high enzymatic activity and yield; by optimizing the selection of carriers and the optimization of induction conditions, large-scale fermentation and industrial production are realized, ferulic acid is released through enzymolysis, high-value utilization of wastes such as straws is promoted, and pollution is reduced.

The invention provides a DNA molecule for coding feruloyl esterase, a recombinant expression vector, a genetically engineered bacterium and application of the genetically engineered bacterium in production of feruloyl esterase, and belongs to the technical field of microbial genetic engineering. The nucleotide sequence of the DNA molecule for coding the feruloyl esterase is as shown in SEQ ID No. 1, and the amino acid sequence correspondingly coded by the DNA molecule is as shown in SEQ ID No. 2. The invention also discloses a recombinant expression vector and a genetically engineered bacterium which comprise the DNA molecule for coding the feruloyl esterase. According to the feruloyl esterase BcFae4711, a strain extracted from soil is used for sequencing, then a corresponding expression vector and engineering bacteria are obtained through genetic engineering modification, and the produced feruloyl esterase has relatively high enzymatic activity and yield; by optimizing the selection of carriers and the optimization of induction conditions, large-scale fermentation and industrial production are realized, ferulic acid is released through enzymolysis, high-value utilization of wastes such as straws is promoted, and pollution is reduced.

The invention discloses a flurbiprofen axetil-containing pharmaceutical composition in the technical field of pharmaceutical preparations. The flurbiprofen axetil-containing pharmaceutical composition comprises flurbiprofen axetil and an intestinal carboxylesterase 2 inhibitor. The intestinal carboxylesterase 2 inhibitor can obviously inhibit intestinal carboxylesterase 2 from hydrolyzing flurbiprofen axetil to release flurbiprofen, so that the local concentration and accumulation of flurbiprofen in gastrointestinal tract tissues are obviously reduced, the damage of flurbiprofen to gastrointestinal mucosa is reduced, the bioavailability of flurbiprofen is improved, the effect is enhanced, the toxicity is reduced, and the preparation method is simple and convenient. The composition is suitable for inflammatory disease and pain management requiring long-term administration.

The invention provides feruloyl esterase, a DNA molecule for coding the feruloyl esterase, a recombinant expression vector, a genetically engineered bacterium and application of the feruloyl esterase in production of the feruloyl esterase, and belongs to the technical field of microbial genetic engineering. The nucleotide sequence of the DNA molecule for coding the feruloyl esterase is as shown in SEQ ID No. 1, and the amino acid sequence correspondingly coded by the DNA molecule is as shown in SEQ ID No. 2. The invention also discloses a recombinant expression vector and a genetically engineered bacterium which comprise the DNA molecule for coding the feruloyl esterase. The feruloyl esterase BgFae2148 is subjected to genetic engineering modification to obtain a corresponding expression vector and an engineering bacterium, and the produced feruloyl esterase has relatively high enzymatic activity and yield; by optimizing the selection of carriers and the optimization of induction conditions, large-scale fermentation and industrial production are realized, ferulic acid is released through enzymolysis, high-value utilization of wastes such as straws is promoted, and pollution is reduced.

This invention relates to a method for preparing feed from leftover food from the dining table, comprising the following steps: fermentation using an enzyme-bacterial compound; the enzyme-bacterial compound includes a compound bacterial agent and a bifunctional enzyme preparation; the bifunctional enzyme preparation is a bifunctional enzyme with xylanase and ferulic acid esterase activities. This invention offers advantages such as high fermentation efficiency, strong process controllability, safe and stable product quality, good anti-rancidity effect, reduced harmful gas emissions, and benefits for environmental protection and resource utilization. The produced semi-digestible feed product has effects such as promoting intestinal peristalsis, stimulating immune function in animals, anti-oxidation, antibacterial and anti-inflammatory effects, anti-cancer effects, and lowering blood lipids, improving nutritional components and feeding effects, and promoting animal health.

The invention discloses a method for flexibly and moderately processing edible oil through biological enzymolysis, and belongs to the technical field of edible vegetable oil processing. The method sequentially comprises the steps of cleaning and color sorting, ozone sterilization, moderate conditioning, biological enzymolysis treatment, moderate aroma enhancement, squeezing at proper temperature, moderate refining, filtering at proper temperature and preservation at proper temperature. The method is characterized in that ultrasonic pretreatment is innovatively introduced after tempering and cooperates with an enzymolysis process that cellulase and feruloyl esterase are compounded according to the mass ratio of 1: 1, and then two-waveband infrared is adopted for aroma enhancement. According to the method, traditional chemical refining is abandoned, the problems of nutrition loss and flavor damage caused by excessive processing are effectively solved, the oil pressing rate and the oil oxidation stability are remarkably improved, active nutritional ingredients such as polyphenols such as vitamin E and squalene are reserved to the maximum extent, and high-valued green processing of oil plants is achieved.

The application provides high-SDF wheat bran powder and a preparation method and application thereof. The steps are as follows: S1. high-quality wheat bran is taken, washed, dried, ultra-finely pulverized and sieved to obtain wheat bran powder; S2. water is added to the wheat bran powder, cellulase is added, ultrasonic treatment is carried out, and enzyme hydrolysis is carried out, 80 DEG C short-time enzyme inactivation is carried out, and the fermentation substrate is obtained after cooling; S3. the water content of the substrate is adjusted, and constant-temperature fermentation is carried out in stages at 37 DEG C: stage one is aerobic fermentation by inoculating Bacillus natto; stage two is anaerobic fermentation by adding sterile water to adjust the water content, introducing nitrogen, inoculating Lactobacillus plantarum and adding food-grade ferulic acid esterase; S4. the fermentation product is subjected to vacuum freeze-drying, ultra-fine pulverization and sieving, and the high-SDF wheat bran powder is obtained. The application breaks through the bottleneck of the disconnection between pretreatment and fermentation, low enzyme utilization rate and non-targeted product conversion in the existing process by using the mild enzyme-preserving pretreatment-bacteria-enzyme synergistic stage-by-stage fermentation technology, realizes efficient value-added modification of wheat bran resources, and expands the application scene of the wheat bran in low GI functional food.

The application belongs to the technical field of biotechnology, and particularly relates to a high-efficiency expression of a Kluyveromyces marxianus strain and application thereof. The Kluyveromyces marxianus strain is obtained by using CRISPR / Cas9 gene editing technology to knock out genes in a protein synthesis metabolic pathway, a secretion and transport pathway, a cytoskeleton synthesis or an intercellular information transmission pathway in a genome of the Kluyveromyces marxianus, and taking the Kluyveromyces marxianus FIM-1 in which a key gene URA3 of a uracil synthesis pathway is knocked out as a starting strain. A FIM-1 ura3Delta mnn11Delta is screened and obtained, and the alpha-galactosidase secretion expression level can be increased by more than 3 times. The Kluyveromyces marxianus strain can be applied to high-efficiency preparation of biological enzymes such as alpha-galactosidase, ferulic acid esterase, chitosanase, saccharifying enzyme, xylanase, mannanase, cellulase and beta-amylase, and has high industrial application value.