43 results about "Acid Esterase" patented technology

The invention belongs to the technical field of antibacterial products, and particularly relates to a female private part antibacterial composition. Through the synergistic effect of the white willow bark extract, the melaleuca alternifolia leaf oil, the sandalwood essential oil and the cedar wood oil, the prepared antibacterial composition has an excellent antibacterial effect, is mild and non-irritant, and can solve the problems that an existing female private part antibacterial agent is limited in antibacterial effect, and the antibacterial effect is poor; and gynecological diseases can be aggravated after long-term use. Wherein the white willow bark extract is prepared through enzymolysis of a compound enzyme composed of cellulase, feruloyl esterase and alkaline protease, the enzymolysis efficiency can be improved, and then the release rate of active ingredients in white willow bark is increased. The preparation method of the antibacterial composition is simple and convenient in operation process and suitable for industrial production. The antibacterial composition is applied to preparation of female private part care products and has good application value.

The present invention relates to fusarium toxin cleavage polypeptide variants of a fusarium toxin carboxylesterase having SEQ ID No.1, 46 wherein said polypeptide variants each have an amino acid sequence shortened by 47 amino acids at the N-terminus and said amino acid sequence has a sequence identity of at least 70%, preferably 80%, particularly preferably 100%, to the amino acid sequence segments 48-540 of SEQ ID No.46, i.e. SEQ ID No.1; and isolated polynucleotides encoding the polypeptide variants; and an additive for cleaving fusarium toxins comprising at least one polypeptide variant and optionally at least one adjuvant; the invention also relates to the use of said polypeptide variants or additives for hydrolytically cleaving at least one fusarium toxin, and to a method for hydrolytically cleaving at least one fusarium toxin.

The invention relates to a high-temperature-resistant feruloyl esterase liquid as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) constructing a reaction system: suspending recombinant microorganism thalli for expressing high-temperature-resistant feruloyl esterase in a buffer solution with the pH value of 7.0-9.0; (2) ultrasonic cracking and thermal purification: heating the reaction system to 70-85 DEG C under the condition of ultrasonic crushing, and keeping the temperature for 1-8 hours; carrying out structural destruction on the microbial thalli by utilizing ultrasound so as to release intracellular high-temperature-resistant feruloyl esterase, and carrying out denaturation and precipitation on host impure protein by utilizing the thermal stability difference between the high-temperature-resistant feruloyl esterase and the host impure protein; after the high-temperature-resistant feruloyl esterase is subjected to heat treatment in the step (2), the catalytic activity is kept at the temperature of 60 DEG C or above; and (3) obtaining an enzyme solution, namely centrifuging the reaction body fluid subjected to the ultrasonic treatment and the heat treatment in the step (2), and collecting supernate, so as to obtain the high-temperature-resistant feruloyl esterase solution.

The present invention relates to fusarium toxin cleavage polypeptide variants of a fusarium toxin carboxylesterase of SEQ ID No. 46 wherein said polypeptide variants each have an amino acid sequence shortened by 47 amino acids at the N-terminus and said amino acid sequence has a sequence identity of at least 70%, preferably 80%, particularly preferably 100%, to the amino acid sequence segments 48-540 of SEQ ID No. 46, i.e. SEQ ID No. 1; and isolated polynucleotides encoding the polypeptide variants; and an additive for cleaving fusarium toxins comprising at least one polypeptide variant and optionally at least one adjuvant; the invention also relates to the use of said polypeptide variants or additives for hydrolytically cleaving at least one fusarium toxin, and to a method for hydrolytically cleaving at least one fusarium toxin.

The invention belongs to the technical field of microorganisms, and provides a high-yield feruloyl esterase plant lactobacillus and application thereof. The bacterial strain is lactobacillus plantarum Fs6, the preservation number of the bacterial strain is CGMCC (China General Microbiological Culture Collection Center) No.36264, and the preservation date of the bacterial strain is October 20, 2025. According to the present invention, the fermentation performance of the dough can be significantly improved with the plant lactobacillus, and the good synergistic effect is represented during the co-fermentation with the yeast; and a good synergistic effect is shown during co-fermentation with yeast. When the mass concentration of bile salt is 0.3 and 0.6 g / L, the survival rate is relatively good, which shows that the lactic acid bacteria Fs6 has a certain probiotic function. The self-aggregation force (32.77%) and the hydrophobicity (32.43%) of the Fs6 are relatively low, and the adhesion of the Fs6 is relatively poor. And combined phenolic acid in the grains can be effectively released, so that the nutritional value of the grains can play a better health role in a human body.

Provided are the screening and identification of a Pseudomonas sp. strain for producing feruloyl esterase at high yield and a use of the Pseudomonas sp. strain as a bioaugmentation agent in fermented foods, relating to the technical field of brewing. By means of screening, a Pseudomonas sp. strain A-4H for producing feruloyl esterase at high yield is obtained. After cultivation for 36 h, the Pseudomonas sp. strain A-4H can produce ferulic acid at a concentration of 153.79±2.36 mg / L, and the activity of feruloyl esterase in a fermentation broth reaches 136.63±10.27 U / L. The addition of the Pseudomonas sp. A-4H during the brewing of Huangjiu can effectively increase the content of ferulic acid, the content of phenolic acid, and the content of organic acid in Huangjiu, providing an effective biological pathway for increasing the content of ferulic acid in fermented foods.

This invention provides a feed additive and compound feed for preventing and treating coccidiosis in broilers, comprising the following components: *Kluyveromyces martensii*, ferulic acid esterase, aminopeptidase, and glucanase. Preferably, the mass percentage of *Kluyveromyces martensii* is 30-40%; the mass percentage of ferulic acid esterase is 15-28%; the mass percentage of aminopeptidase is 15-25%; and the mass percentage of glucanase is 18-25%. Based on extensive research, this invention has determined that this combination has a significant effect on coccidiosis control in broilers, effectively improving broiler production performance indicators, increasing economic benefits, and reducing the use of anticoccidial drugs.

The application belongs to the technical field of cosmetics and skin external preparation, and discloses a retinoid, a preparation method and application thereof. The compound is formed by covalently coupling a Schiff base or a kinin releasing enzyme cleavable peptide bond (L) and a free L-amino acid, a di- to hexapeptide or a C6-C22 fatty acid ester (P) of the retinol parent (R), and the structural general formula is R-L-P. The compound has a 12-week stability rate of ≥80% in a 25℃ aqueous preparation, is recognized by a keratin layer carboxylic acid esterase and a kinin releasing enzyme KLK-5 / KLK-7 after being applied to the skin, is subjected to enzymatic cutting in a skin acidic cuticle (pH 4.5-5.5) microenvironment, and releases free retinol at a fixed point. Compared with retinol palmitate, the in-situ release efficiency of the compound of the application is increased by 2.5-4.5 times, while the risk of systemic absorption is significantly reduced, and the incidence of sensitive skin irritation is ≤6.7%.

The invention relates to an application of carboxylesterase TfdI in degradation of phenoxy carboxylic acid herbicides. The invention provides application of rhodococcus pyridinivorans Y6 in degrading phenoxy carboxylic acid herbicides, a novel phenoxy carboxylic acid herbicide degrading enzyme TfdI is cloned from the rhodococcus pyridinivorans Y6, the rhodococcus pyridinivorans Y6 has excellent catalytic action on phenoxy carboxylic acid herbicides, the degradation rate on 2, 4-D isooctyl ester with the concentration of 100 mg / L within 1 h is 100%, and the degradation effect is stable. The excellent degrading enzyme provided by the invention has good application potential in bioremediation of the environment polluted by the phenoxy carboxylic acid herbicide, a new scheme is provided for solving the problem of residual pollution of the 2, 4-D isooctyl ester herbicide in agricultural production, and meanwhile, a herbicide degradation gene resource library is enriched.

Methods of treating a metabolic disorder in a subject are provided. Aspects of the method include enhancing extracellular Carboxylesterase 2 (CES2) activity in the subject in order to treat the subject for the metabolic disorder. Also provided are compositions for use in practicing the methods.

The application discloses a carboxylic esterase lyophilization protective agent, a preparation method and application thereof, and belongs to the technical field of bioengineering. The carboxylic esterase lyophilization protective agent is a single-component polyvinylpyrrolidone; wherein, the polyvinylpyrrolidone has a viscosity-average molecular weight Mv of 10000-15000000; a K value of 17-90; when used for lyophilization protection of carboxylic esterase, the carboxylic esterase lyophilized powder can effectively improve the stability of the carboxylic esterase lyophilized powder at normal temperature, prevent the purity and activity of the carboxylic esterase lyophilized powder from decreasing, and the lyophilized powder cake is complete, easy to be powdered, the enzyme powder resuspension is clear and transparent, the enzyme activity recovery rate is high, and the carboxylic esterase is convenient for being applied to different application scenarios.

The invention provides a high temperature resistant feruloyl esterase and an application thereof. The feruloyl esterase wild type has a remarkable high-temperature-resistant characteristic and still shows relatively high thermal stability under the condition of 95 DEG C, and the thermal inactivation half-life period of the feruloyl esterase wild type can reach 5h. On the basis, after the feruloyl esterase mutant obtained through structural rationality modification continuously reacts for 5 h at the temperature of 75 DEG C, floccule precipitation is not observed, and stable catalytic activity is kept. In the application, the feruloyl esterase mutant disclosed by the invention and commercial xylanase have a synergistic effect, so that the release of bound ferulic acid in starch-removed wheat bran can be effectively promoted. The invention provides a novel enzymology tool for efficiently and quickly releasing the plant-derived ferulic acid under a high-temperature condition.

The invention belongs to the field of biological enzyme catalysis, and provides a carboxylesterase CarEst3 mutant, the mutant is obtained by mutating phenylalanine at the 66th site of an amino acid sequence as shown in SEQ ID NO.2 into arginine, and the mutant can be used for catalyzing kinetic resolution of racemization 3-cyclohexene-1-methyl formate to prepare (S)-3-cyclohexene-1-formic acid. High stereoselectivity is achieved, and large-scale production can be achieved; the method for preparing (S)-3-cyclohexene-1-formic acid has the advantages of mild reaction conditions, high substrate concentration, good stereoselectivity, simple post-treatment, high product yield and the like.

This invention discloses a near-infrared fluorescent probe for detecting bone regeneration vitality and its preparation method, belonging to the field of biochemistry. Using rhodamine as the fluorescent core, the invention utilizes quinoline ring expansion to increase the conjugated area and extend emission. The brightness and stability of the probe are controlled by adjusting the steric hindrance of the aromatic ring at position 9, constructing a series of stable, high-brightness probe cores with low pKa. By introducing a phosphate ester group as a responder while simultaneously quenching the fluorescence of the core, a near-infrared fluorescent probe, SiRd-MOP, for detecting bone regeneration vitality is obtained. The preparation method of this invention has high yield and mild reaction conditions. The prepared probe can specifically and sensitively respond to alkaline phosphatase (ALP), and has successfully achieved ALP activity monitoring in osteogenic differentiation models and throughout the entire process of fracture regeneration and repair in mice. It can be used for real-time assessment of bone regeneration vitality and has very high biological application prospects.

The invention provides a biosynthesis method of an esdoxaban tosylate chiral intermediate. According to the preparation method, 3-cyclohexene-1-carboxylic acid methyl ester is taken as a starting raw material, and a target intermediate is finally obtained through carboxylesterase resolution, hydrolysis, hydroxylation, dehydrogenation oxidation, transamination reaction, amidation and Boc2O reaction in sequence. According to the method, high-selectivity carboxylesterase is used for realizing chiral resolution; p450 oxidase with excellent regioselectivity and stereoselectivity is utilized to realize specific hydroxylation of a C-H bond under a mild condition, and a traditional process depending on a highly toxic chemical oxidant is overturned; then, clean oxidation is realized through alcohol dehydrogenase, and a way is laid for subsequent reaction; most importantly, the transaminase is adopted to directly construct the chiral amine, so that the use of a high-risk reagent sodium azide is thoroughly avoided, and major potential safety hazards are eliminated from the source; and finally, green amidation is realized by virtue of lipase, and an acyl chloride method for generating corrosive wastewater is replaced.

The present invention provides compositions used for the treatment and / or prevention of protein aggregation disorders. [Solution] Proteopathy encompasses a wide range of ailments, including neurodegenerative diseases (e.g., polyglutamine diseases such as huntingtin in Alzheimer's disease, Parkinson's disease, and Huntington's disease, and prion diseases); amyloidosis of other non-neuronal proteins (especially I1-antitrypsin, immunoglobulin light and heavy chains, lactadherin, apolipoprotein, gelzolin, lysozyme, fibrinogen, atrial natriuretic factor, keratin, lactoferrin, and β-2 microglobulin, etc.); sickle cell disease; cataracts; cystic fibrosis; retinitis pigmentosa; and nephrogenic diabetes insipidus. Administration of sulfatase inhibitors is generally suitable for treating and / or preventing protein toxicity associated with proteopathy. Therefore, the present invention provides compositions comprising sulfatase inhibitors for the treatment of proteopathy.

The present disclosure provides engineered carboxylesterase enzymes having improved activity in catalyzing cyclization of short polypeptides, as compared to a naturally occurring wild-type carboxylesterase enzyme. These variants catalyze the cyclization of linear tetrapeptides with high regioselectivity. Also provided are polynucleotides and expression vectors encoding the carboxylesterase enzymes and methods of using the carboxylesterase enzymes to generate cyclic tetrapeptides containing non-canonical amino acids.

The present invention discloses a novel camphor-based fluorescent probe for detecting the organophosphorus pesticide dimethoate, as well as its preparation method and application. The present invention uses camphor as a raw material, condenses it with ethyl isonicotinate to obtain 3-(pyridine-4-formyl)-1,7,7-trimethylbicyclo[2.2.1]2-heptanone, and then cyclizes it with 4-bromophenylhydrazine to obtain 2-(4-bromophenyl)-7,8,8-trimethyl-3-(pyridine-4-yl)-4,5,6,7-tetrahydro-2H-4,7-bridged methylindazole, which is then coupled with 4-formylphenylboronic acid to obtain 4 ′ ‑(7,8,8-trimethyl-3-(pyridin-4-yl)-4,5,6,7-tetrahydro-2H-4,7-methyleneindazol-2-yl)-[1,1′-diphenyl]-4-yl)methylene)benzofuran-3(2H)-one, which was then esterified with acetyl chloride. 3-Oxo-2-((4′-(7,8,8-trimethyl-3-(pyridin-4-yl)-4,5,6,7-tetrahydro-2H-4,7-methyleneindazol-2-yl)-[1,1′-diphenyl]-4-yl)methenyl)-2,3-dihydrobenzofuran-6-yl acetate (abbreviated as CPA-OAc) was obtained. This compound can undergo enzymatic hydrolysis with carboxylesterase. Under ultraviolet irradiation, the fluorescent color of the solution changes from colorless to green. Since dimethoate can effectively inhibit the activity of carboxylesterase, when dimethoate is added to the solution, the fluorescence of the solution changes from green to colorless. Therefore, this compound can be used as a fluorescent probe for detecting dimethoate.

The present invention provides a method for synthesizing phenylpropenyl esters using ferulic acid esterase. The method comprises reacting methyl phenylpropenyl ester and ethyl phenylpropenyl ester with an alcohol or a linear fatty alcohol containing a phenyl or naphthyl group. The method is mild and environmentally friendly, has simple steps, uses a cost-effective substrate, produces few by-products, is conducive to industrial production, and is suitable for widespread application.

The invention relates to the technical field of microbial application and bioengineering, and discloses an aspergillus awamori SNJ-2F capable of simultaneously producing feruloyl esterase, cellulase and xylanase at high yield, which is separated from Shennongjia high-temperature yeast for making hard liquor and preserved in China Center for Type Culture Collection with the preservation number of CCTCC NO: M20252024. The invention also discloses a screening method of the strain, a bran seed preparation process and a method for producing ferulic acid by performing enzymolysis on starch-removed wheat bran with a bran seed crude enzyme. The strain disclosed by the invention is strong in enzyme system synergy, green in process and low in cost, can be used for ferulic acid preparation, white spirit / soy sauce brewing and feed industry, and has a wide application prospect.

The invention discloses a near-infrared fluorescent probe for detecting bone regeneration activity and a preparation method of the near-infrared fluorescent probe, and relates to the technical field of biochemistry. According to the invention, rhodamine is taken as a fluorescent parent nucleus, quinoline ring expansion is utilized to enlarge the conjugate area and prolong emission, and the brightness and stability of the probe are regulated by regulating the steric hindrance of a ninth aromatic ring, so that a series of stable and high-brightness probe parent nucleuses with smaller pKa are constructed. The near-infrared fluorescent probe SiRd-MOP for detecting the bone regeneration activity is obtained by introducing a phosphate ester group as a response group and quenching parent nucleus fluorescence at the same time. The preparation method is high in yield and mild in reaction condition, the prepared probe can specifically and sensitively respond to alkaline phosphatase (ALP), ALP activity monitoring in the whole process of fracture regeneration and repair of a cell osteogenic differentiation model and a mouse is successfully achieved, the probe can be used for evaluating bone regeneration activity in real time, and the probe has very high biological application prospects.

The present invention provides a near-infrared fluorescent probe for detecting carboxylesterase and exhibiting antibacterial activity, as well as its preparation method and application. The probe has the following structural formula: #imgabs0#, wherein R is selected from #imgabs1#, #imgabs2#, #imgabs3#, #imgabs4#, #imgabs5#, or #imgabs6#. The probe of the present invention utilizes a hemicyanine derivative as a parent nucleus, introduces p-aminobenzenesulfonamide as an antibacterial active group, and utilizes acid esters containing different R groups as recognition groups for detecting carboxylesterase. Carboxylesterase-induced ester cleavage is accompanied by a significant enhancement in the fluorescence signal. The probe can effectively detect carboxylesterase from different proteases, amino acids, and yin and yang samples, exhibiting good selectivity and high sensitivity. The probe's action on carboxylesterase is accompanied by a distinct color change visible to the naked eye, and the probe exhibits a certain degree of antibacterial activity, resulting in successful applications for the detection and imaging of carboxylesterase in biological systems.

The application discloses a recombinant feruloyl esterase and a mutant and application thereof, and belongs to the technical field of enzyme engineering. Clostridium butyricum, The wild-type enzyme amino acid sequence is shown as SEQ ID NO:1, and the coding gene sequence is shown as SEQ ID NO:2; the target enzyme is obtained by constructing a recombinant expression vector containing the CbFAE gene, inducing expression in Escherichia coli BL21 (DE3) and purifying; substrate spectrum analysis shows that the enzyme not only has catalytic decomposition activity of model substrates such as methyl ferulate, but also has catalytic activity of decomposing mono (2-hydroxyethyl) terephthalate (MHET) into terephthalic acid (TPA) and ethylene glycol (EG); the application also constructs the CbFAE mutant L168R based on sequence and structure analysis, the amino acid sequence of the mutant enzyme is shown as SEQ ID NO:3, and the catalytic MHET decomposition activity of the mutant enzyme is increased by nearly 4 times compared with the wild type under the same reaction condition. The recombinant feruloyl esterase and the mutant provided by the application expand the types of MHET hydrolytic enzymes and provide a new way for the biodegradation of MHET.

The invention provides a DNA molecule for coding feruloyl esterase, a recombinant expression vector, a genetically engineered bacterium and application of the genetically engineered bacterium in production of feruloyl esterase, and belongs to the technical field of microbial genetic engineering. The nucleotide sequence of the DNA molecule for coding the feruloyl esterase is as shown in SEQ ID No. 1, and the amino acid sequence correspondingly coded by the DNA molecule is as shown in SEQ ID No. 2. The invention also discloses a recombinant expression vector and a genetically engineered bacterium which comprise the DNA molecule for coding the feruloyl esterase. According to the feruloyl esterase BccFae3074, a strain extracted from soil is used for sequencing, then a corresponding expression vector and engineering bacteria are obtained through genetic engineering modification, and the produced feruloyl esterase has relatively high enzymatic activity and yield; by optimizing the selection of carriers and the optimization of induction conditions, large-scale fermentation and industrial production are realized, ferulic acid is released through enzymolysis, high-value utilization of wastes such as straws is promoted, and pollution is reduced.

The invention provides a DNA molecule for coding feruloyl esterase, a recombinant expression vector, a genetically engineered bacterium and application of the genetically engineered bacterium in production of feruloyl esterase, and belongs to the technical field of microbial genetic engineering. The nucleotide sequence of the DNA molecule for coding the feruloyl esterase is as shown in SEQ ID No. 1, and the amino acid sequence correspondingly coded by the DNA molecule is as shown in SEQ ID No. 2. The invention also discloses a recombinant expression vector and a genetically engineered bacterium which comprise the DNA molecule for coding the feruloyl esterase. According to the feruloyl esterase BcFae4711, a strain extracted from soil is used for sequencing, then a corresponding expression vector and engineering bacteria are obtained through genetic engineering modification, and the produced feruloyl esterase has relatively high enzymatic activity and yield; by optimizing the selection of carriers and the optimization of induction conditions, large-scale fermentation and industrial production are realized, ferulic acid is released through enzymolysis, high-value utilization of wastes such as straws is promoted, and pollution is reduced.

The invention belongs to the technical field of genetic engineering and biology, and particularly discloses construction and application of a xylan feruloyl esterase delivery plasmid. The amino acid sequence of the xylan feruloyl esterase is shown as SEQ ID NO: 1, a xylan feruloyl esterase delivery plasmid pQ-XFE based on IncQ type plasmid and a recombinant genetic engineering bacterium are constructed, and thus the xylan feruloyl esterase is obtained. According to the delivery plasmid, efficient and stable delivery of xylan feruloyl esterase genes in various microorganisms is achieved, the enzyme expression quantity and activity can be remarkably improved, and the application range of the xylan feruloyl esterase genes is widened. The recombinant xylan feruloyl esterase has a remarkable degradation effect on ester compounds and can be applied to the fields of food, pharmacy, papermaking, feed and the like due to the specific activity and enzymatic characteristics of the recombinant xylan feruloyl esterase.

The application provides ferulic acid esterase mutants with improved specific activity and application thereof, and the application carries out directional evolution on a ferulic acid esterase gene derived from Bacillus pumilus, expresses the ferulic acid esterase gene in an E. coli expression system, and obtains ferulic acid esterase mutants DV1 and DV2 with improved enzyme activity and expression efficiency through screening. The amino acid sequences of the ferulic acid esterase mutants DV1 and DV2 are shown in SEQ ID NO. 4 and SEQ ID NO. 6 respectively, and the mutants DV1 and DV2 obtained by the application have higher protein yield, higher specific activity of methyl ferulate esterase, and higher degradation efficiency of macromolecular p-nitrophenol ester substrates compared with the original bacteria BpFAE. Therefore, the mutants can be used for degrading plant fibers and preparing feed fermenting agents, and have good market application prospect.

The invention discloses a flurbiprofen axetil-containing pharmaceutical composition in the technical field of pharmaceutical preparations. The flurbiprofen axetil-containing pharmaceutical composition comprises flurbiprofen axetil and an intestinal carboxylesterase 2 inhibitor. The intestinal carboxylesterase 2 inhibitor can obviously inhibit intestinal carboxylesterase 2 from hydrolyzing flurbiprofen axetil to release flurbiprofen, so that the local concentration and accumulation of flurbiprofen in gastrointestinal tract tissues are obviously reduced, the damage of flurbiprofen to gastrointestinal mucosa is reduced, the bioavailability of flurbiprofen is improved, the effect is enhanced, the toxicity is reduced, and the preparation method is simple and convenient. The composition is suitable for inflammatory disease and pain management requiring long-term administration.

The invention provides feruloyl esterase, a DNA molecule for coding the feruloyl esterase, a recombinant expression vector, a genetically engineered bacterium and application of the feruloyl esterase in production of the feruloyl esterase, and belongs to the technical field of microbial genetic engineering. The nucleotide sequence of the DNA molecule for coding the feruloyl esterase is as shown in SEQ ID No. 1, and the amino acid sequence correspondingly coded by the DNA molecule is as shown in SEQ ID No. 2. The invention also discloses a recombinant expression vector and a genetically engineered bacterium which comprise the DNA molecule for coding the feruloyl esterase. The feruloyl esterase BgFae2148 is subjected to genetic engineering modification to obtain a corresponding expression vector and an engineering bacterium, and the produced feruloyl esterase has relatively high enzymatic activity and yield; by optimizing the selection of carriers and the optimization of induction conditions, large-scale fermentation and industrial production are realized, ferulic acid is released through enzymolysis, high-value utilization of wastes such as straws is promoted, and pollution is reduced.

The invention relates to the technical field of enzyme engineering and biology, and discloses carboxylesterase as well as a coding gene, a recombinant vector, a recombinant strain, an enzyme preparation and application thereof. The carboxylesterase (GutestA) has an amino acid sequence as shown in SEQ ID NO. 1 (sequence identifier number 1). When the GutestA takes an acid ester compound as a substrate, the GutestA is high in specific enzyme activity, high in thermal stability, wide in pH application range and high in catalytic efficiency, and has a good industrial application prospect in the fields of drug synthesis, industrial catalysis, chiral compound resolution, grease processing, environmental governance and the like.