The invention discloses a
small molecule compound combination and a method for preparing vascular endothelial cells by using the
small molecule compound combination to induce differentiated cells. According to the method, differentiated cells are subjected to targeted induction so as to finally obtain vascular endothelial cells, wherein the targeted induction comprises: inhibiting the activity oflysine
deacetylase activity, inhibiting the signaling pathway of TGF-beta, inhibiting the activity of
DNA methyltransferase (DNMT), activating the signaling pathway of WNT / beta-
catenin, and activatingthe signaling pathway of cAMP. According to the present invention, through the induction with the
small molecule compound combination, the differentiated cells can be transdifferentiated into the vascular endothelial cells, each step can be subjected to precise
quality control, and the standardized operation and the large-scale production can be conveniently achieved; and the donor source of themethod is wide, the patient himself can be served as the donor, and the vascular endothelial cells required by
basic research,
clinical treatment or
tissue engineering production can be obtained within a short time.