127results about How to "Biosafety" patented technology

The invention provides a cellulose / two-dimensional layered material composite hydrogel. The composite hydrogel comprises a cellulose three-dimensional network structure and a two-dimensional materialsupported in the cellulose three-dimensional network structure, wherein the two-dimensional layered material is a two-dimensional transition metal carbide, nitride, or carbonitride. According to the composite hydrogel provided by the invention, the two-dimensional layered material can be stably supported in the composite hydrogel system and is not easy to agglomerate; and the composite hydrogel has good compatibility with a biological fluid, and the advantages of full biodegradability and high biological safety, and can be used in the field of biomedicines. The invention also provides a preparation method of the composite hydrogel.

The invention discloses bacillus pumilus, a probiotics preparation and a preparation method and application thereof. The Bacillus pumilus LV149 is preserved in China center for type culture collection (CCTCC) in Nov. 23th, 2011, and the preservation number is CCTCC NO: M 2011411. The bacillus pumilus LV149 has strong extracellular protease, lipase and amylase activities, has wide rejection capability to vibrio and has no hemolytic activity. The Bacillus pumilus LV149 serves as fermenting bacterial strains and is performed with solid fermentation, drying and smashing so as to prepare bacillus pumilus probiotics preparation which uses Bacillus pumilus LV149 as the active ingredients. The bacillus pumilus probiotics preparation can be added into prawn feeds for feeding prawns, so that growth of prawn intestinal pathogenic vibrio can be restrained, prawn vibriosis can be reduced, simultaneously prawn growth is promoted, fish bait coefficient is reduced, quality of commodity is improved, culture cycle is shortened, accordingly culture risk and culture cost are reduced, biological safety is high and the bacillus pumilus, the probiotics preparation and the preparation method and application thereof have wide application prospect in aquaculture.

The invention provides a bacillus subtilis xylose induced secretion expression vector pXYL-amyQ. The expression vector is constructed by using bacillus subtilis plasmids pHT43 as starting plasmids, removing original promoters and a repressor gene sequence thereof and inserting the bacillus subtilis plasmids into an expression box of bacillus subtilis xylose promoters and a signal peptide sequence. The xylose promoter expression box comprises a xylose induced promoter sequence and a repressor gene sequence thereof. The constructed expression vector pXYL-amyQ can secrete expression homogenous and heterogenous proteins in bacillus subtilis. The host bacillus subtilis of the vector is a generally recognized as safe (GRAS) host, and the adopted xylose serving as an inducer is nontoxic, so the expression vector has biological safety and plays an important role in protein expression, particularly enzyme and antibody preparation.

The invention discloses a Zn-Li-Mg-system zinc alloy and a preparation method and application thereof. The zinc alloy comprises, by weight, 0-1% of Li, 0-1% of Mg and the balance Zn, wherein the masspercents of Li and Mg are not zero. The zinc alloy prepared by the preparation method is excellent in mechanical performance, can provide long-term and effective supporting force in a body, has excellent cellular and blood compatibility, and can be applied to preparation of biomedical implants.

The invention discloses a Mg(OH)2 / Mg-Sn hydrotalcite composite film having an intercalation structure on the surface of magnesium and magnesium alloy and a preparation method thereof, relates to the technical field of the surface of magnesium and magnesium alloy, and aims to improve the corrosion resistance of magnesium and magnesium alloy and widen the application prospects in the fields of biological medicine and the like. The preparation method comprises the following steps: S1, initial treatment of magnesium and magnesium alloy; S2, pretreatment: preparing a Sn4<+> salt solution, regulating the pH value to 4.0 to obtain a pretreatment solution, and putting the magnesium and magnesium alloy in the pretreatment solution with continuously introduced CO2 to obtain a precursor film; S3, posttreatment: soaking the magnesium and magnesium alloy coated with the precursor film in a Na2CO3 solution to grow a Mg(OH)2 / Mg-Sn hydrotalcite composite film on the surface of the magnesium and magnesium alloy; and S4, subsequent cleaning. According to the invention, the Mg(OH)2 / Mg-Sn hydrotalcite composite film containing no aluminum and having biosafety is grown on the surface of magnesium and magnesium alloy in an in-situ generation manner through a two-step method; the corrosion resistance of magnesium and magnesium alloy is improved; and the application of the hydrotalcite film in biological magnesium alloy surface protection is widened.

The invention discloses a preparation method of a pervaporation composite membrane filled with hydrophobically modified nano calcium carbonate. The preparation method of the pervaporation composite membrane filled with hydrophobically modified nano calcium carbonate comprises the following steps: (1) adding dried nano calcium carbonate into oleic acid, stirring for reacting, then repeatedly washing with hexane for removing excess oleic acid,, drying in vacuum, and grinding so as to obtain hydrophobically modified nano calcium carbonate; (2) mixing dried polyvinylidene fluoride with hydrophobically modified nano calcium carbonate, subsequently adding the mixture into dimethylacetamide, carrying out ultrasonic dispersion, stirring, filtering, standing and defoaming, and subsequently pouring the obtained liquid on a polyester non-woven fabric for scraping a membrane so as to obtain a modified polyvinylidene fluoride base membrane by using an immersion precipitation phase inversion method; and (3) dissolving polydimethylsiloxane in n-hexane, adding hydrophobically modified nano calcium carbonate, carrying out ultrasonic dispersion, adding a cross-linking agent and a catalyst, stirring for reacting, centrifuging and dedoaming, subsequently pouring the obtained liquid on the polyvinylidene fluoride base membrane for scraping the membrane, airing and drying the membrane in vacuum to prepare the pervaporation composite membrane. The composite membrane prepared by the method is simple in process and low in cost, and moreover, the selectivity and the flux of the composite membrane on alcohol are improved.

The invention discloses a Zn-Li-Mn-based zinc alloy and a preparation method and application thereof. The Zn-Li-Mn-based zinc alloy comprises, by mass, greater than 0 and less than or equal to 1% of Li, greater than 0 and less than or equal to 1% of Mn and the balance of Zn. The Zn-Li-Mn-based zinc alloy has excellent mechanical properties, can provide long-term effective support in the human body, has excellent cell compatibility and blood compatibility and can be used for preparation of biomedical implants.

The invention relates to an activated toxic antibody kit for detecting a porcine reproductive and respiratory syndrome virus, which is characterized by being implemented by comprising the following steps of: after diluting a serum sample diluent to be detected twice, adding 100 microliters into an ELISA (Enzyme-Linked Immuno Sorbent Assay) plate for hatching at 37 DEG C for 1 hour; setting positive control, negative control and blank control; cleaning for five times with washing liquid every two minutes; then adding 100 microliters into each combined monoclonal antibody hole, hatching at 37 DEG C for 1 hour and cleaning for five times with washing liquid every two minutes; then adding goat-antimouse IgG-HRP ligature for acting at 37 DEG C for 1 hour and cleaning for five times with washing liquid every two minutes; then adding a developing substrate solution; mixing the substrate A with the substrate B according to the proportion of 1:1; adding 100 microliters into the ELISA hole; developing for 15 minutes by keeping in dark place; and adding 50 microliters of stop solution and detecting the OD490 value. The judging result is set according to the principle that the sample corresponding to the inhibition ratio PI which is equal to (OD uninhibited-OD sample) / OD uninhibited*100 percent, PI) 30 percent is positive, and the sample smaller than 30 percent is negative. The kit method has mature technology and strong repeatability, can effectively distinguish porcine reproductive and respiratory syndrome virus active toxic and inactivated toxic antibody and can be finished just by a common researcher.

The invention discloses a method for preparing a compound water purifying agent for emergency treatment of water pollution of a drinking water source, which comprises the following steps of: grinding concave soil raw ore into fine powder, and sieving by using a sieve with 100 to 300 meshes to obtain concave soil powder; calcining the concave soil powder at the temperature of between 350 and 500 DEG C for 1 to 5 hours, or radiating the concave soil powder by using microwaves with the power of 300 to 600W for 5 to 15 minutes to obtain modified concave soil; and mixing the modified concave soil and an inorganic polymer coagulant accounting for 5 to10 weight percent of the modified concave soil and potassium permanganate accounting for 1 to 5 weight percent of the modified concave soil in aqueous solution, stirring for uniform dispersion, drying, and grinding to obtain the water purifying agent finished product. The compound water purifying agent can obviously reduce the content of organic matters, algae toxins, heavy metals and the like in the water of the polluted drinking water source, the manufacturing cost is low, the operating process is simple, the sedimentation velocity is high, the sludge volume is small, the residues of inorganic reagents are a few, and the compound water purifying agent has chemical and biological safety and is a novel environment-friendly and high-efficiency water purifying agent for emergency treatment of the water pollution of the drinking water source.

The invention discloses a Zn-RE series zinc alloy as well as a preparation method and application thereof. The main element of the material of the Zn-RE series zinc alloy is Zn, and the alloy elementis one of non-radioactive rare earth elements RE; the mass percentage of RE is 0%-3% but not including 0; the balance is zinc; and the non-radioactive rare earth element RE comprises Sc, Y, 139La, Ce,Pr, Nd, 150Sm, Gd, Tb, Dy, Ho, Er, Tm, Yb and 175Lu. According to the Zn-RE series zinc alloy as well as the preparation method and application thereof, compared with rare earth-containing multi-element zinc alloy in the prior art, the alloy components of the Zn-RE series zinc alloy are reduced, the preparation process flow is simplified, the adjustment of the microstructure in the material and the regulation and control of the performance can be realized through different processing technologies, the mechanical property is greatly improved, meanwhile, the biocompatibility is good, and the Zn-RE series zinc alloy can be used for preparing degradable medical implants.

The invention discloses a multi-mode ionization source, a multi-mode ionization source system, a mass spectrum, and a method for analyzing a to-be-tested sample with the mass spectrum. The multi-mode ionization source comprises a capillary tube, an internal electrode, an insulating tube, an external electrode, a tee fitting, an insulating sleeve, a metal ring, a conductive member and a regulating member, wherein the outlet of the internal electrode in the axial direction is located between the insulating tube and the outlet of the outer electrode. The multi-mode ionization source can be easily switched between two modes of electrospray ionization and plasma ionization, which can effectively improve the efficiency of analysis and detection, avoid the complicated dismounting process of the ionization source and realize the highly sensitive detection of components in the complex sample. The multi-mode ionization source is especially suitable for a variety of polar and non-polar small molecule compounds.

The invention discloses a load enzyme hydrogel composition with oxygen releasing function and hydrogel prepared from the hydrogel composition, the the hydrogel composition contains a component A and a component B, wherein the active components of the component A are glucose oxidase, a catalyst for catalyzing hydrogen peroxide to produce oxygen, and hydrophilic colloid; the active components of the component B are glucose and a cross linking agent. The preparation method of the hydrogel is even mixing of the component A and the component B. The load enzyme hydrogel composition adopts raw materials belonging to the food or medical materials with high biological safety and good biocompatibility; and before use, the two components are not contacted and stable in performance. The hydrogel prepared by mixing of the hydrogel composition can continuously supply oxygen, can increase the oxygen partial pressure at the wound, improves the wound tissue oxygen supply, improves the local tissue aerobic metabolism, is conducive to healing, enables the oxygen to reach to the wound through the body outer surface other than blood circulation, is a local oxygen supply technology and easy to carry, and has wide popularization and application prospect.

The present invention belongs to the field of biological preventing and controlling of weeds. The strain for preventing and controlling weed alligator alternanthera belongs to Fusarium and has the preservation number of CGMCC No. 0922. It has strong parasitic, high alligator alternanthera preventing and controlling effect to above ground part and underground part of alligator alternanthera, and no pathogenicity to tested crops, is environment safe. It may be used to replace available pesticide with serious pollution. It may be used via spraying with the fungus spore concentration being 105 / ml. After being applied, it can kill over 95 % of malignant weed alligator alternanthera in 5-10 days.

The invention discloses a temperature-sensitive postoperative adhesion cellulose modifying material and a preparation method and application thereof. Sulfobetaines type zwitterionic monomers with anti-cell-adhesion or protein non-specificity adsorption property are grafted and co-polymerized onto a temperature-sensitive methylcellulose main chain, and a grafted copolymer aqueous solution is prepared by a low-temperature dissolution method. At the room temperature, the grafted copolymer aqueous solution is viscous liquid which is easy to inject or coat, can become hydrogel spontaneously and rapidly at the temperature of the human body, and has reversible sol-gel phase transformation characteristic. The temperature-sensitive postoperative adhesion cellulose modifying material is good in biological compatibility, and high in cell adhesion resistance and tissue adhesion resistance, is suitable for adhesion prevention of complicated surgical wounds or wound parts, and has high operation convenience and postoperative adhesion prevention effectiveness.

The invention relates to the fields of a medical material and an apparatus thereof, and provides an in-vivo degradable implantable intravascular stent product. The product comprises 1) an in-vivo degradable implantable zinc-based metal material; 2) the intravascular stent product produced by the zinc-based alloy material in the step 1) is in a pipenet structure; 3) a magnesium metal film coating is uniformly distributed and deposed at the surface of the pipenet stent product; and 4) the magnesium film coating is coated with a degradable polymer coating, and the polymer coating can contains therapeutic drugs. The zinc based alloy has the advantages of excellent mechanical properties, strong corrosion controllability, and excellent compatibility. According to the intravascular stent product,surface special arrangement is benefit for keeping a complete initial structure, is benefit for stent endothelialization, reduces an inflammatory reaction, and reduces local inflammation, restenosisand thrombus risk in the stent after stent implantation.

The invention relates to a preparation method of a long-chain alkane quaternization antibacterial agent, and a product and application of the antibacterial agent. Long-chain haloalkane is modified ona polymer richly containing tertiary amine; the carbon number on alkyl of the long-chain haloalkane is not smaller than 7. The prepared antibacterial agent has an excellent antibacterial effect on staphylococcus aureus, escherichia coli, candida albicans, helicobacter pylori, pseudomonas aeruginosa, aspergillus flavus and listeria monocytogenes; in the concentration dose with the antibacterial effect, cytotoxicity cannot be generated; the biological safety is realized. The preparation method is safe and simple and can be applied to industrial production; the development and application have development prospects.

The invention discloses a composition of plant direct gene transformation, transforming method and appliance, which is characterized by the following: making the component include goal gene and free nucleic acid component of adjusted and controlled element, transforming buffer liquid and transforming assistance component; co-culturing; soaking; surface-daubing; injecting; dripping; spraying; transforming with dip flower method or through genetic gun bombardment, supersonic wave and electric shock method. This component can be used to receptor plant cell direct transformation of fertilized ovum, suspended cell, protoplast, single-layer cell and diachyma cell and can be used to seed, receptor plant pollen, pistil, flower pillar, pollen pipe and ovary, which possesses impotent meaning for plant genetic seeding, variety improvement and gene functional analysis.

The invention discloses an immunofluorescence reagent for detecting an E-type enterovirus and a detection kit thereof. A direct immunofluorescence detection method set up by applying the immunofluorescence reagent for detecting the E-type enterovirus can be used for clinically fast diagnosing E-type enterovirus infection and used for positioning and authenticating an E-type enterovirus antigen in tissue. The time for direct immunofluorescence detection is short, and a result can be reported within 40 min; specificity is high, and the immunofluorescence reagent only reacts with the E-type enterovirus; the immunofluorescence reagent is high in repeatability and accuracy, the coincidence rate between results of immunofluorescence reagent and results of an indirect immunofluorescence method is 90% or more, and the immunofluorescence reagent can be used for fast diagnosing E-type enterovirus infection.

The invention provides an embolism microsphere based on soluble starch as well as preparation and application of the embolism microsphere. The embolization microsphere is prepared from soluble starch, an olefin polar monomer, an initiator and a cross-linking agent through a reversed-phase suspension polymerization technology, a continuous phase is an oil phase and a surfactant, and a dispersed phase is the soluble starch, the olefin polar monomer, the initiator and the cross-linking agent. According to the embolism microsphere, the olefin polar monomers and the soluble starch are subjected to free radical grafting and then cross-linking to obtain embolism microspheres, the embolism microspheres are of rich porous structures, pores extend towards the interiors of the microspheres, the specific surface area of the embolism microspheres is large, the surfaces of the embolism microspheres have a large number of hydrophilic polar groups, and the embolism microspheres can generate electrostatic adsorption with drugs, and a good drug sustained-release effect of the embolism microsphere is realized. The doxorubicin hydrochloride drug loading capacity of the embolism microspheres prepared by the method is as high as 191mg / g, the encapsulation efficiency is as high as 96%, and the drug loading capacity and the encapsulation efficiency of the starch embolism microspheres are effectively improved.

The invention relates to macromolecular fire retarding and extinguishing gel and a preparation method thereof. The preparation method of the macromolecular fire retarding and extinguishing gel comprises the following steps: 1, preparing a neutralization solution; 2, adding a crosslinking agent, an initiator and an adhesion promoter; 3, performing polymerization reaction; 4, dispersing generated high-water-absorption resin into plant oil; 5, adding a stabilizing agent into suspension. The gel prepared by the preparation method disclosed by the invention is always in a uniformly dispersed liquid state; during use, the macromolecular fire retarding and extinguishing gel can be directly siphoned into a water tank without stirring and mixing and then directly sprayed without waiting; the gel cannot be blocked and aggregated, is high in dispersivity, is damage-free to spraying equipment, cannot delay a fire extinguishing fighter, and fundamentally overcomes the shortcoming of an existing powdered solid product; furthermore, the macromolecular fire retarding and extinguishing gel can be sprayed for use.

The invention discloses a preparation method of oxalate decarboxylase. The method comprises the following steps: constructing a recombinant expression plasmid vector comprising Bacillus-containing acid induced promoter, a secretion signal peptide, and a fungus-derived oxalate decarboxylase gene prepared under the control of the secretion signal peptide, transforming bacilli normally growing in a pH value being not more than 4.5 by the vector to prepare oxalate decarboxylase recombinant bacteria; and carrying out culture and acid feeding on the recombinant bacteria in a fermentation medium, inducing the recombinant bacteria, and carrying out simple purification to recover generated extracellular oxalate decarboxylase. The extracellular oxalate decarboxylase prepared through the method is used in diagnosis reagent raw materials, medicinal compositions, healthcare foods or formulated foods with special medical uses. The mutated acid induced promoter is adopted to substitute an original acid induced promoter in the recombinant expression vector, so the transformed bacilli have a high expression level.

The invention discloses a G type enterovirus detecting immunofluorescent reagent and a G type enterovirus immunofluorescent detecting kit. The immunofluorescent reagent is a VP1 monoclonal antibody capable of being labeled with a fluorescent dye, the VP1 monoclonal antibody is obtained by utilizing VP1 protein of the G type enterovirus and adopting a hybridoma monoclonal antibody preparation technology, and the amino acid sequence of the VP1 protein is shown as the sequence table SEQ ID NO.2. The immunofluorescent reagent and the detecting kit can be used for rapid diagnosis of G type enterovirus infection in clinic, and can also be used for localization and identification of a G type enterovirus pathogen in tissue; the direct immunofluorescent detecting time is short, and report results can be generated in 40 min; the specificity is strong, and reaction can only be conducted with the G type enterovirus; the repeatability is good, the accuracy is high, the coincidence rate to an indirect immunofluorescence method can reach 90% or above, and the immunofluorescent reagent and the detecting kit can be used for rapid diagnosis of the G type enterovirus infection.

The invention relates to a method for implementing gene engineering genetic improvement on a gp64-free baculovirus of a genome by using a Bac-to-Bac system. The method comprises construction of a recombinant virus, amplification of a recombinant virus polyhedrosis and biological assay of the recombinant virus; and according to the construction strategy, a gp64 gene controlled by an autographa californica multiplenucleopolyhedrovirus (AcMNPV) polyhedrosis virus gene promoter and a cyst membrane glycoprotein gene promoter of an orgyia pseudotsugata multiplenucleopolyhedrovirus (OpMNPV) polyhedrosis virus together and a helicoverpa armigera single nucleopolyhedrovirus (HearNPV) polyhedrin gene controlled by an AcMNPV polyhedrin gene promoter and a HearNPV polyhedrin gene promoter together are inserted into a polyhedrin gene site of HearNPV bacmid to construct a recombinant HearNPV bacmid for co-expressing two cyst membrane glycoproteins, namely GP64 and F proteins. The recombinant virus has better insecticidal activity and higher biological safety than a wild virus.

The invention discloses a double-antibody sandwich ELISA kit for detecting an E-type enterovirus pathogen. VP1 protein expressed by a VP1 gene with the base sequence shown as a sequence table SEQ ID NO.1 is adopted to immunize BALB / C mice to prepare a polyclonal antibody serving as a capture antibody, VP2 protein expressed by a VP2 gene with the base sequence shown as a sequence table SEQ ID NO.3 is adopted to immunize BALB / C mice to prepare a monoclonal antibody, and horse radish peroxidase (HRP) is marked. Safety is high, and the coincidence rate between kit detection results and RT-PCR detection results is 100%. The kit can be stored for 6 months at the temperature of 4 DEG C, and no obvious difference exists between detection results and those of a conventional method.

The invention discloses a prcK gene knocked-out suicide plasmid pYTKLKRT and a construction method thereof, relating to a suicide plasmid and a construction method thereof. The prcK gene knocked-out suicide plasmid pYTKLKRT is composed of a left fragment prcKL of a prcK gene, a right fragment prcKR of the prcK gene, a tetracycline resistance gene and a plasmid pUC18. The construction method comprises the steps: firstly, amplifying the fragment prcKL; secondly, obtaining a plasmid pUC18-KL; thirdly, amplifying the fragment prcKR; fourthly, thus obtaining a plasmid pUC18-KLKR; fifthly, amplifying the tetracycline resistance gene Tet; and sixthly, obtaining the prcK gene knocked-out suicide plasmid pYTKLKRT. The prcK gene knocked-out suicide plasmid pYTKLKRT and the construction method thereof are mainly applied to the field of bioengineering research.

The invention relates to colloidal gold quick diagnosis test paper for distinguishing classic porcine reproductive and respiratory syndrome (PRRS) from highly pathogenic PRRS (HPRRS). The test paper is characterized in that a sample pad, a bonding pad, a nitrocellulose membrane and an absorption pad are sequentially adhered to a nonabsorbent support sheet; the bonding pad is coated with a colloidal gold labeled classic PRRS virus (PRRSV) peculiar antigen; and the nitrocellulose membrane is respectively coated with a detection line, namely a T line consisting of staphylococcal protein A (SPA), and a quality control line, namely a C line consisting of a classic PRRS monoclonal antibody 2B9. The test paper has the obvious advantages of high specificity, sensitivity and diagnosis speed, is easy to operate, can be used for distinguishing classic PRRSV from HPRRSV, and is used for the quick diagnosis of PRRSV.

The invention discloses a lactobacillus plantarum producing exopolysaccharides, and belongs to the technical field of microorganisms. The lactobacillus plantarum JNULCC001 has biosafety and does not generate toxins, and with the strain as a fermentation strain and whey powder as a raw material, functional fermented products can be produced. The produced fermented whey products are uniform in texture, good in sensory state, good in rheological property, capable of generating characteristic flavor matter, good in post-curing stability and high in viable count. Besides, in the method of producingthe fermented products, it is not needed to add any exogenous food additive, the recycling of whey is realized, the problems of environmental pollution and resource waste are relieved, and the methodhas environmental friendliness and realizes sustainable development.

The invention provides a microbial fertilizer, a preparation method and an application thereof. The microbial fertilizer comprises fungus species LP-20 and organic fertilizers, wherein the organic fertilizers comprise humic acid, turfy soil and composted chicken manure. The preparation method of the microbial fertilizer comprises the following steps: culturing the fungus species LP-20 to obtain a bacterial suspension; concentrating the obtain bacterial suspension to obtain a bacteria concentrate, and uniformly mixing the bacteria concentrate with the humic acid, the turfy soil and the composted chicken manure; pelletizing and drying to obtain the microbial fertilizer. The microbial fertilizer prepared from the fungus species LP-20 and the organic fertilizers can be applied to treatment of heavy metal pollution of soil, can effectively enrich free cadmium ions and zinc ions in the soil, has double effect of being taken as a production-increasing and fertilizer-maintaining fertilizer and a natural conditioner for treating the heavy metal pollution of the soil, and is an environmental friendly and ecological-coordination microbial fertilizer for treating the heavy metal pollution of the soil.

The invention relates to a preparation method using bacterial lysate as inactivated vaccine adjuvant. The preparation method comprises the following steps: step 1, respectively putting corynebacterium pseudotuberculosis and rhodococcus equi into an LB culture medium for culturing at 37 DEG C, and harvesting thalli through a centrifugal machine; step 2, diluting each thallus to 8.0* 10<9> cfu / mL by using sterilized normal saline, and crushing the bacterial liquid by using a high-pressure homogenizer; and step 3, after sterility test, mixing the split products of corynebacterium pseudotuberculosis and rhodococcus equi with the two bacteria inactivated by formaldehyde according to a ratio of 1: 1 to obtain the inactivated vaccine added with the adjuvant. The preparation method has the characteristics of safety, high efficiency, strong immune enhancement capability and low cost, and is suitable for producing various bacterial inactivated vaccines.