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Strain and fungus agent for preventingand controlling malignant weed alternathera philoxeroides

A vicious weed, water peanut technology, applied in application, biocide, plant growth regulator, etc., to achieve good control effect, strong parasitic specialization, and fast speed.

Inactive Publication Date: 2004-04-28
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the control effect of pathogenic bacteria on water peanuts is affected by factors such as the environment, dosage form, and the host range of the pathogen itself, there is currently no mature commercial product for the biological control of water peanuts.

Method used

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  • Strain and fungus agent for preventingand controlling malignant weed alternathera philoxeroides
  • Strain and fungus agent for preventingand controlling malignant weed alternathera philoxeroides
  • Strain and fungus agent for preventingand controlling malignant weed alternathera philoxeroides

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0027] Preparation of biocontrol agents: Transfer the isolated Fusarium strain WNJ01 to a PDA plate, and after cultivating in the dark at 25°C for 5 days, cut 20 pieces of 1×1mm mycelium along the edge of the colony and transfer them to the PDA liquid medium After 10 days of cultivation at 25°C and in the dark with shaking (80rpm), measure the concentration of conidia in the culture solution, and adjust the concentration of spores to 10 7 spores / ml, and stored at room temperature for later use.

[0028] Application method: Dilute the above bacterial agents 100 times to 10 5 spores / ml, add 2% Tween-80 solution, and when the humidity is relatively high in the evening, the 2 Apply 300mL of consumption to spray on water peanuts, after about 3 days, the aerial parts of water peanut plants are completely rotten, and after 7 days, the underground roots are completely rotten. image 3 .

Embodiment 1

[0029] Example 1 Control of land water peanuts:

[0030] The living environment of water peanuts includes both land and water environments. The bacterium agent provided by the present invention is diluted 100 times, to 10 5 spores / ml, add 2% Tween 80, and when the humidity is relatively high in the evening, the 2 Spray 300mL on water peanuts. After 1 day, obvious brown necrosis spots appear on the tender leaves of water peanuts. After about 5 days, the aboveground parts of water peanut plants are completely rotten. After 10 days, the underground roots are completely rotten, which is harmful to the farmland environment. The control effect of Caoshui Peanut is more than 95%.

example 2

[0031] Example 2 Prevention and control of water peanuts in waters and rivers:

[0032] The bacterium agent provided by the present invention is diluted 100 times, to 10 5 spores / ml, add 2% Tween 80, in the evening or early morning, according to every 10m 2 Spray 300mL on water peanuts. After 1 day, obvious brown necrosis spots appear on the young leaves of water peanuts. After about 4 days, the aboveground parts of the water peanut plants are completely rotten. After about 10 days, the roots in the water are completely rotten, which is harmful to the farmland environment. The control effect of weeds and peanuts reaches more than 95%.

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Abstract

The present invention belongs to the field of biological preventing and controlling of weeds. The strain for preventing and controlling weed alligator alternanthera belongs to Fusarium and has the preservation number of CGMCC No. 0922. It has strong parasitic, high alligator alternanthera preventing and controlling effect to above ground part and underground part of alligator alternanthera, and no pathogenicity to tested crops, is environment safe. It may be used to replace available pesticide with serious pollution. It may be used via spraying with the fungus spore concentration being 105 / ml. After being applied, it can kill over 95 % of malignant weed alligator alternanthera in 5-10 days.

Description

(1) Technical field [0001] The invention discloses a bacterial strain, bacterial agent and application for preventing and controlling the malignant weed water peanut, and belongs to the field of biological control. It is specially used for the safe control of water peanuts, and the obtained biological agent is a new high-efficiency herbicide. In today's serious pesticide pollution, it provides a good substitute for chemical pesticides. (2) Background technology [0002] Water peanut, scientific name Alternantliera philoxeroides Griseb, also known as Alternantliera philoxeroides Griseb, is a perennial herbaceous plant of the genus Alternamthera in the family Amaranthaecae. Water peanuts were introduced to my country in the late 1930s. Because water peanuts have strong vitality, adapt to aquatic and terrestrial environments, grow and reproduce rapidly, and are artificially introduced and spread, the breeding and spread of water peanuts in my country is very fast. At present, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00
Inventor 王源超郑小波郑燕梅张正光
Owner NANJING AGRICULTURAL UNIVERSITY
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