Lactobacillus plantarum producing exopolysaccharides

A technology of Lactobacillus plantarum and whey, applied in the field of microorganisms, can solve the problems of high cost, no treatment system, pollution, etc., and achieve the effect of improving flavor substances and good product properties

Active Publication Date: 2019-08-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional aerobic process of whey treatment is very costly (Enzyme and Microbial Technology, 2007, 41:876-880), and most dairy factories do not have corresponding treatment systems, therefore, about 47% of whey is discharged to rivers or land to cause serious pollution problems (Biotechnology Progress, 2004, 20:1091-1095), which also caused a waste of nutrients

Method used

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  • Lactobacillus plantarum producing exopolysaccharides
  • Lactobacillus plantarum producing exopolysaccharides
  • Lactobacillus plantarum producing exopolysaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Isolation and screening of exopolysaccharide-producing lactobacillus

[0046] (1) Dilution of sample solution: Use a sterile 1mL pipette to draw 1mL sample solution into a sterile small test tube and dilute it with sterile physiological saline to a dilution of 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 , 10 -7 , 10 -8 , 10 -9 , 10 -10 , 10 -11 , 10 -12 . Choose a dilution of 10 -4 to 10 -12 The diluent is ready for use.

[0047] (2) Separation of plates: first, under the condition of aseptic operation on the ultra-clean bench, the sterilized MRS solid medium containing calcium carbonate is poured onto the plate, and after the temperature is room temperature, the above dilution is drawn with a pipette gun. 10 -4 to 10 -12 0.1mL of fermentation broth. Pouring and coating onto the culture dish of the MRS solid medium containing calcium carbonate by pouring method and coating method respectively, each dilution was repeated twice, and packed wit...

Embodiment 2

[0050] Embodiment 2 monosaccharide composition analysis

[0051] According to the "monosaccharide composition analysis method" in the specific embodiment, the monosaccharide composition is analyzed, and the results are as follows Figure 4 As shown, the extracted polysaccharides are mainly composed of glucose, mannose, galactose and galacturonic acid, with contents of 260, 27, 82 and 20 μmol / g, respectively.

Embodiment 3

[0052] The identification of embodiment 3 bacterial strains

[0053] (1) Bacterial morphology observation and Gram staining

[0054] Use an inoculation loop to pick up a small amount of bacterial samples in the ultra-clean bench and spread them evenly on the glass slide, then heat to kill the bacteria with an alcohol lamp, add ammonium oxalate crystal violet dropwise, and stain for 1 min. Rinse off excess staining solution on the washed slides with water, and blot dry with absorbent paper. Add a small amount of iodine-potassium iodide solution dropwise, let stand for 1min, then wash with water, blot dry with absorbent paper, add ethanol continuously dropwise for decolorization, until the outflowing liquid is colorless, then wash with water. Finally, counterstain with saffron stain for 30 s. After staining, observe with a microscope, Gram-positive bacteria are purple, and Gram-negative bacteria are red. As a result of the identification, the isolated lactic acid bacteria are...

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Abstract

The invention discloses a lactobacillus plantarum producing exopolysaccharides, and belongs to the technical field of microorganisms. The lactobacillus plantarum JNULCC001 has biosafety and does not generate toxins, and with the strain as a fermentation strain and whey powder as a raw material, functional fermented products can be produced. The produced fermented whey products are uniform in texture, good in sensory state, good in rheological property, capable of generating characteristic flavor matter, good in post-curing stability and high in viable count. Besides, in the method of producingthe fermented products, it is not needed to add any exogenous food additive, the recycling of whey is realized, the problems of environmental pollution and resource waste are relieved, and the methodhas environmental friendliness and realizes sustainable development.

Description

technical field [0001] The invention relates to a plant lactobacillus producing exopolysaccharide, which belongs to the technical field of microorganisms. Background technique [0002] Every year, a large amount of food waste and food residues are produced all over the world, which not only causes environmental pollution but also brings huge economic losses. With the increase of people's preference for fermented milk products and the development of dairy enterprises, a large amount of whey and other liquid residues are produced (Food & Function, 2018). Whey accounts for 85-95% of the whole milk volume (Bioresource technology, 1996, 57: 1-11), and is considered as a substance rich in nutritional value because it contains 55% of dairy nutrients. Whey contains 93-94% water and lactose, protein, minerals, lactic acid and fat (Journal of environmental management, 2012, 110:48-68.). [0003] The traditional aerobic process of whey treatment is very costly (Enzyme and Microbial T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04A23C21/02A23L29/00A23L33/135A23K10/18C12R1/25
CPCC12N1/20C12P19/04A23C21/02A23L29/065A23L33/135A23K10/18A23V2002/00C12R2001/25C12N1/205A23V2400/169A23V2200/30
Inventor 李程程
Owner JIANGNAN UNIV
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