Temperature-sensitive postoperative adhesion cellulose modifying material and preparation method and application thereof

A modified material and cellulose technology, applied in surgery, medical science, bandages, etc., can solve the problems of long blocking time, easy loss, difficult operation, etc., and achieve the effect of low equipment requirements, low price and wide applicability

Active Publication Date: 2017-02-15
SUN YAT SEN UNIV +1
View PDF5 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commonly used anti-adhesion barrier materials mainly contain the following three categories: one is solid film materials, such as a postoperative anti-adhesion film material disclosed in patent CN103044700A, a postoperative anti-adhesion polymer film disclosed in patent CN104436317A, etc., such materials Because it has been preformed, it is not easy to operate during the operation and it is difficult to apply to some complicated wound sites, so its application is limited; the second type is water liquid substances, such as a kind of anti-adhesion washing liquid for surgery disclosed in patent CN103271939A, this type The material has good fluidity and can be injected or applied to complex wound sites, but it is easy to lose, the barrier time is not long enough, and the effect is limited; the third is hydrogel subst

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Temperature-sensitive postoperative adhesion cellulose modifying material and preparation method and application thereof
  • Temperature-sensitive postoperative adhesion cellulose modifying material and preparation method and application thereof
  • Temperature-sensitive postoperative adhesion cellulose modifying material and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Weigh 1.0 g of methyl cellulose (MC) with a viscosity of 15 cP and add it to 50 mL of distilled water at 80° C., then place the flask in an ice-water bath and continue stirring to dissolve it completely, and it becomes a transparent liquid. Then weigh 0.5 g of methacryloyl ethyl sulfobetaine (DMAPS) into the flask and continue to stir, and pass nitrogen protection. Then add 50 mg of ammonium persulfate (APS) as an initiator and 65 microliters of N,N,N',N'-tetramethylethylenediamine (TEMED, density 0.775g / mL, the same below) as an accelerator. The reaction was carried out at 25° C. for 24 hours under a nitrogen atmosphere. After the reaction, all the reaction solution was transferred to a dialysis bag with a molecular weight cut-off of 8000, dialyzed with deionized water for 3 days, and freeze-dried to obtain methyl cellulose grafted methacryloyl ethyl sulfobetaine zwitterionic single body copolymer.

[0037] (2) Weigh 1 gram of methylcellulose grafted methacryloyl...

Embodiment 2

[0040](1) Weigh 1.2 g of methyl cellulose (MC) with a viscosity of 15 cP and add it to 50 mL of distilled water at 80° C., then place the flask in an ice-water bath and continue to stir to dissolve it completely, and it becomes a transparent liquid. Then weigh 0.3 g of methacryloyl ethyl sulfobetaine (DMAPS) into the flask and continue to stir, and pass nitrogen protection. Then add 30 mg of ammonium persulfate (APS) as an initiator and 40 microliters of N,N,N', N'-tetramethylethylenediamine (TEMED) as an accelerator, and react at 45°C under a nitrogen atmosphere for 12 Hour. After the reaction, all the reaction solution was transferred to a dialysis bag with a molecular weight cut-off of 8000, dialyzed with deionized water for 3 days, and freeze-dried to obtain methyl cellulose grafted methacryloyl ethyl sulfobetaine zwitterionic single body copolymer.

[0041] (2) Weigh 1 gram of methylcellulose grafted methacryloyl ethyl sulfobetaine zwitterionic monomer copolymer prepare...

Embodiment 3

[0046] (1) Referring to Example 2, an aqueous solution of methyl cellulose grafted methacryloyl ethyl sulfobetaine zwitterionic monomer copolymer with a mass fraction of 10% was prepared and stored at 4°C.

[0047] (2) Weigh 1 gram of methylcellulose with a viscosity of 15 cP, add 10 ml of disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution with pH=7.4, place in an ice-water bath and stir continuously to dissolve it. After being placed overnight in a refrigerator at 4°C to allow air bubbles to escape, a 10% aqueous solution of methylcellulose was obtained and stored at 4°C.

[0048] (3) Use methylcellulose aqueous solution and methylcellulose grafted methacryloyl ethyl sulfobetaine zwitterionic monomer copolymer aqueous solution to evenly cover half the area of ​​the cell culture plate, and the other half area of ​​the culture plate is covered with As a blank control. Place the culture plate in a cell culture incubator at 37°C to allow the gel samples to f...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Viscosityaaaaaaaaaa
Login to view more

Abstract

The invention discloses a temperature-sensitive postoperative adhesion cellulose modifying material and a preparation method and application thereof. Sulfobetaines type zwitterionic monomers with anti-cell-adhesion or protein non-specificity adsorption property are grafted and co-polymerized onto a temperature-sensitive methylcellulose main chain, and a grafted copolymer aqueous solution is prepared by a low-temperature dissolution method. At the room temperature, the grafted copolymer aqueous solution is viscous liquid which is easy to inject or coat, can become hydrogel spontaneously and rapidly at the temperature of the human body, and has reversible sol-gel phase transformation characteristic. The temperature-sensitive postoperative adhesion cellulose modifying material is good in biological compatibility, and high in cell adhesion resistance and tissue adhesion resistance, is suitable for adhesion prevention of complicated surgical wounds or wound parts, and has high operation convenience and postoperative adhesion prevention effectiveness.

Description

technical field [0001] The invention belongs to the technical field of preparation of biomedical hydrogel materials, and in particular relates to a temperature-sensitive anti-postoperative adhesion cellulose modified material and a preparation method and application thereof. Background technique [0002] With the development of a large number of surgical operations, the problem of postoperative biological tissue adhesion has become increasingly prominent. Adhesions are generally abnormal structures formed by the abnormal connection between the wound site and surrounding tissues or organs caused by trauma, infection or other factors after surgery. Postoperative adhesions not only affect the effect of surgery, but also lead to serious postoperative complications, such as small bowel obstruction, pelvic adhesions in women, etc., which make the operation fail. Therefore, finding effective ways to prevent postoperative adhesions has always been an important topic in the field of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C08F251/02C08F220/38A61L31/14A61L31/04A61L26/00
CPCA61L26/0023A61L26/0061A61L26/008A61L26/009A61L31/042A61L31/14A61L31/145A61L31/148C08F251/02
Inventor 张黎明张俊钊李海量黄雅清谢民强
Owner SUN YAT SEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap