Microbial fertilizer, preparation method and application thereof
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A microbial fertilizer and microorganism technology, applied in the field of microorganisms, can solve problems such as heavy metal pollution, and achieve the effect of promoting adsorption
Inactive Publication Date: 2015-04-29
CECEP L&T ENVIRONMENTAL TECH
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CN 1490280A discloses a microbial multi-antibacterial fertilizer and its preparation method and application. The microbial multi-antibacterial fertilizer made of bacteria and fungi with different effects is a microbial fertilizer with nutritional and biological control functions, but it is not aimed at heavy metal pollution. governance
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Embodiment 1
[0035] The adsorption effect of fungal strains to heavy metal cadmium and zinc in soil in the bacterial fertilizer of embodiment 1
[0036] In the present embodiment, the fungal strain LP-20 is cultivated by the following method, comprising the following steps:
[0037] (a) Petri dish culture: inoculate the fungal strain LP-20 on a solid medium under sterile conditions, and cultivate at 25°C for 5 days;
[0038] (b) primary seed cultivation: inoculate the bacteria cultured in step (a) in a liquid medium under aseptic conditions, and cultivate them for 5 days at 25° C. to obtain primary seeds;
[0039] (c) Secondary seed cultivation: the volume ratio of the liquid medium is 10% inoculum size, inoculating the first-order seeds into a 5L fermenter, aerating, and cultivating for 6 days to obtain the second-order seeds;
[0040] (d) Mixed fermentation culture: the volume ratio of the liquid medium is 10% inoculum amount, inoculate the secondary seeds into the fermenter (mechanic...
Embodiment 2
[0048] Example 2 The different mixing ratios of strains and organic fertilizers on the adsorption of heavy metal cadmium in soil by microbial fertilizers and the effect of zinc
[0049] First, prepare microbial fertilizer samples 1#, 2#, 3#, 4#, 5# and 6#, wherein the 1# sample is prepared as follows: as in Example 1, the LP-20 bacterial suspension is obtained, and it is centrifuged , to obtain concentrated thallus, get concentrated thalline 0.01g, it is mixed with 1g humic acid, 5g peat soil and 3.99g decomposed chicken manure to make microbial fertilizer 1# sample (thalline content in microbial fertilizer is 0.1 %); 2# sample is prepared as follows: cultivate LP-20 bacterium suspension as embodiment 1, carry out centrifugation to it, obtain concentrated thalline, get concentrated thalline 0.1g, with 1g humic acid, 5g peat Soil and 3.9g decomposed chicken manure are mixed and made into microbial fertilizer 2# sample (thalline content is 1% in the microbial fertilizer); 3#...
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Abstract
The invention provides a microbial fertilizer, a preparation method and an application thereof. The microbial fertilizer comprises fungus species LP-20 and organic fertilizers, wherein the organic fertilizers comprise humic acid, turfy soil and composted chicken manure. The preparation method of the microbial fertilizer comprises the following steps: culturing the fungus species LP-20 to obtain a bacterial suspension; concentrating the obtain bacterial suspension to obtain a bacteria concentrate, and uniformly mixing the bacteria concentrate with the humic acid, the turfy soil and the composted chicken manure; pelletizing and drying to obtain the microbial fertilizer. The microbial fertilizer prepared from the fungus species LP-20 and the organic fertilizers can be applied to treatment of heavy metal pollution of soil, can effectively enrich free cadmium ions and zinc ions in the soil, has double effect of being taken as a production-increasing and fertilizer-maintaining fertilizer and a natural conditioner for treating the heavy metal pollution of the soil, and is an environmental friendly and ecological-coordination microbial fertilizer for treating the heavy metal pollution of the soil.
Description
technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a microbial fertilizer, a preparation method and an application thereof. Background technique [0002] In recent decades, various environmental problems have become more and more serious threats to the development of human beings. Various industrial waste gases, waste water, chemical fertilizers, pesticides and other pollutants have entered the soil of their final hosts, resulting in a decline in the quality of the soil environment, especially It is soil heavy metal pollution, which has become a hot spot of common concern of the government and society. [0003] Due to the poor structure of heavy metal polluted soil and the lack of nutrients, heavy metals exist in a highly toxic form, which affects plant growth, and heavy metal ions inhibit or activate soil enzyme activities, thereby affecting the release of soil nutrients and the absorption of nutrients by crops. Cadmium is...
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