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Loaded enzyme hydrogel composition with oxygen releasing function and hydrogel prepared from hydrogel composition

A composition and hydrogel technology, applied in the direction of drug combination, organic active ingredients, inorganic active ingredients, etc., to achieve the effect of improving oxygen supply, beneficial to healing, and good biocompatibility

Active Publication Date: 2014-10-29
GUANGDONG PROV MEDICAL INSTR INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, from a biosafety point of view, most reactions are not acceptable

Method used

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  • Loaded enzyme hydrogel composition with oxygen releasing function and hydrogel prepared from hydrogel composition
  • Loaded enzyme hydrogel composition with oxygen releasing function and hydrogel prepared from hydrogel composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Add 8g of guar gum to 50mL of deionized water, heat to dissolve, add 1.5g of sodium alginate to dissolve after cooling to room temperature, add 0.04g of glucose oxidase and 0.04g of catalase under stirring conditions, mix well, and form a viscose Thick aqueous solution, that is, component A (containing 800μg / mL glucose oxidase, 800μg / mL catalase, 190g / L hydrocolloid);

[0036] Add 0.8 g of borax to 50 mL of deionized water, dissolve, then add 6 g of glucose under stirring conditions, mix well to form an aqueous solution, which is component B (containing 120 g / L glucose, 16 g / L cross-linking agent);

[0037] Mix components A and B evenly to obtain a hydrogel, and use an oxygen electrode to measure its oxygen sustained release effect. After component A and component B are mixed in a closed flask, the oxygen concentration change is measured by an oxygen concentration measuring instrument, and the measurement results are as follows: figure 1 shown.

Embodiment 2

[0039] Add 0.3 g of polyvinyl alcohol to 50 mL of deionized water, heat to dissolve, add 0.2 g of sodium alginate to dissolve after cooling to room temperature, add 0.1 g of glucose oxidase and 0.1 g of catalase under stirring conditions, mix well, and form Viscous aqueous solution, that is, component A (containing 2000μg / mL glucose oxidase, 2000μg / mL catalase, 10g / L hydrocolloid);

[0040] Add 0.1 g of borax to 50 mL of deionized water, dissolve, then add 0.5 g of glucose and 0.5 g of carbamide peroxide under stirring conditions, and mix well to form an aqueous solution, which is component B (containing 10 g / L glucose, 2 g / L cross-linked agent);

[0041] Mix components A and B evenly to obtain a hydrogel, and use an oxygen electrode to measure its oxygen sustained release effect.

Embodiment 3

[0043] Add 150g of gelatin to 500mL of deionized water, heat to dissolve, add 50g of oxidized starch to dissolve after cooling to room temperature, add 0.005g of glucose oxidase and 0.005g of catalase under stirring conditions, and mix well to form a viscous aqueous solution, namely Component A (containing 10μg / mL glucose oxidase, 10μg / mL catalase, 300g / L hydrocolloid);

[0044] Add 40 g of borax to 500 mL of deionized water, dissolve, then add 200 g of glucose under stirring conditions, and mix well to form an aqueous solution, which is component B (containing 400 g / L glucose and 80 g / L cross-linking agent);

[0045] Mix components A and B evenly to obtain a hydrogel, and use an oxygen electrode to measure its oxygen sustained release effect.

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Abstract

The invention discloses a load enzyme hydrogel composition with oxygen releasing function and hydrogel prepared from the hydrogel composition, the the hydrogel composition contains a component A and a component B, wherein the active components of the component A are glucose oxidase, a catalyst for catalyzing hydrogen peroxide to produce oxygen, and hydrophilic colloid; the active components of the component B are glucose and a cross linking agent. The preparation method of the hydrogel is even mixing of the component A and the component B. The load enzyme hydrogel composition adopts raw materials belonging to the food or medical materials with high biological safety and good biocompatibility; and before use, the two components are not contacted and stable in performance. The hydrogel prepared by mixing of the hydrogel composition can continuously supply oxygen, can increase the oxygen partial pressure at the wound, improves the wound tissue oxygen supply, improves the local tissue aerobic metabolism, is conducive to healing, enables the oxygen to reach to the wound through the body outer surface other than blood circulation, is a local oxygen supply technology and easy to carry, and has wide popularization and application prospect.

Description

technical field [0001] The invention relates to an enzyme-loaded hydrogel composition with oxygen-releasing function and the hydrogel formed therefrom. Background technique [0002] With the development of wound healing theory, the effect of oxygen on wound healing has been increasingly understood. An important reason why chronic wounds such as diabetic feet are difficult to heal is that the lack of blood supply to the wound site prevents oxygen from being delivered to the wound through the blood. Another example is when the wound is narrow and deep, ischemic, with many necrotic tissues, poor drainage, and mixed with other aerobic bacteria infection to cause local hypoxia of the wound, tetanus is just prone to occur. The role of oxygen in the wound healing process is multifaceted. Wound healing is an energy-consuming process. Oxygen can provide the energy support needed for the process; promote the synthesis of collagen and the formation of new blood vessels in the process ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/44A61K33/00A61K9/06A61P17/02A61K31/7004
Inventor 刘群峰陈军张佳泳许少波张泽荣张锦耿志杰
Owner GUANGDONG PROV MEDICAL INSTR INST
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