99results about How to "High hydrolytic activity" patented technology

Compositions and methods are provided for enhancing saccharification of biomass with one or more enzymes to enhance availability of substrates for fermentation by a microorganism. Microorganisms are also modified to enhance activity of one or more hydrolytic enzymes that are present endogenously in or are introduced heterologously into a host microorganism.

The invention belongs to the technical field of sulfur reclamation in the rubber ingredient industry, and in particular relates to a method for filling a sulfur production catalyst in the rubber ingredient industry, which is characterized in that the sulfur production catalyst is respectively filled into triple-stage sulfur production reactors, wherein the height of the catalyst in the triple-stage sulfur production reactors is all 800 millimeter; the upper part of a primary converter is filled with 1 cubic meter of PSR-41, and the lower part of the primary converter is filled with 2 cubic meters of PSR-31; the upper part of a secondary converter is filled with 1.1 cubic meters of the PSR-41, and the lower part of the secondary converter is filled with 2.1 cubic meters of the PSR-31; and the upper part of a tertiary converter is filled with 1.6 cubic meters of the PSR-41, and the lower part of the tertiary converter is filled with 1.6 cubic meters of the PSR-31. The method improves the sulfur conversion rate of a sulfur reclamation plant in the rubber industry.

The invention provides a method for producing fermented beef jerky with compound leaven. The bacteria strain used in fermentation is lactobacillus casei and staphylococcus xylosus with volume ratio of 1:3 and volume of addition of 1-1.5%, the fermentation temperature is 20 DEG C, and the fermentation time is 5 days. The addition of lactobacillus casei reduces pH value of the product, improves safety, improves the texture of the product, and shortens fermentation period. At the same time, the addition of staphylococcus xylosus generates a large amount of flavoring substances during fermentation process, improves sensing effect of the product, and the final product has thick fragrance, good taste, and infinitive aftertaste. The inventive method is especially suitable for producing fermented beef jerky, as well as air dried sausage and sausage.

The invention discloses a higher-activity partial glyceride lipase mutant and application thereof. The mutant is an enzyme mutant prepared by carrying out site-directed mutagenesis on Malassezia globosa partial glyceride lipase, wherein the mutant site is Phe at the 278th site, and the Phe at the 278th site is mutated into Ile or Gly. The obtained mutant Phe278Ile or Phe278Gly has higher partial glyceride hydrolysis activity, and is more suitable for application in biochemical engineering industry. The partial glyceride hydrolysis activities of the modified SMG1Phe278Ile and SMG1Phe278Gly mutants are enhanced to different degrees, which are respectively 1.84 times and 1.68 times of the wild type SMG1 lipase hydrolysis activity; and the modified SMG1Phe278Ile and SMG1Phe278Gly mutants have higher partial glyceride activity, and can be used for removing partial glyceride in grease.

The invention belongs to the technical field of sulfur reclamation in the rubber ingredient industry, and in particular relates to a method for filling a sulfur production catalyst in the rubber ingredient industry, which is characterized in that the sulfur production catalyst is respectively filled into triple-stage sulfur production reactors, wherein the height of the catalyst in the triple-stage sulfur production reactors is all 800 millimeter; the upper part of a primary converter is filled with 1 cubic meter of PSR-41, and the lower part of the primary converter is filled with 2 cubic meters of PSR-31; the upper part of a secondary converter is filled with 1.1 cubic meters of the PSR-41, and the lower part of the secondary converter is filled with 2.1 cubic meters of the PSR-31; and the upper part of a tertiary converter is filled with 1.6 cubic meters of the PSR-41, and the lower part of the tertiary converter is filled with 1.6 cubic meters of the PSR-31. The method improves the sulfur conversion rate of a sulfur reclamation plant in the rubber industry.

The present invention relates to methods and enzymes for degrading hydrophobic ester pesticides and toxins. In particular, the present invention relates to the use of insect esterases, and mutants thereof, in the bioremediation of hydrophobic ester pesticides and toxins residues, such as pyrethroid residues, contaminating the environment and horticultural commodities.

The invention relates to a catalyst used for removing carbonyl sulfide from chemical production raw gas, in particular to a COS hydrolysis catalyst comprising components of magnesium-aluminum-based hydrotalcite, gamma-Al2O3 and TiO2, and a preparation method thereof. The preparation method comprises the following steps of: uniformly mixing the gamma-Al2O3, TiO2 and magnesium-aluminum-based hydrotalcite, adding water for kneading, extruding into strips, drying and roasting to prepare the COS hydrolysis catalyst product. The invention has the advantages that: the hydrotalcite is roasted at high temperature to form a compound oxide with high specific surface area and strong basicity, which can meet the requirements on pore diameter and suitable basicity of the COS hydrolysis catalyst; meanwhile, the prepared catalyst is not needed to be impregnated with alkali metal solution, and has high hydrolysis activity and high stability; and the preparation process is simple, the cost is low and the invention has obvious economic benefit.

The present invention relates to the use of insect esterases or lipases, or mutants thereof, as catalysts in biotransformation processes. The present invention may have application in any process involving hydrolysis, esterification, transesterification, interesterification or acylation reactions. The invention also has application in the enzymatic resolution of compounds to produce optically active compounds and has particular, but not exclusive, application to substrates having a hydrophobic moiety such as pyrethroids and fatty acid esters.

The invention discloses a sulfonated carbonaceous solid acid catalyst as well as a preparation method and application thereof to a microcrystalline cellulose hydrolysis process. The density of a sulfonic acid group on the surface of the catalyst is 0.30 to 0.80mmol / g and the density of an oxygen-containing group is 1.00 to 2.50mmol / g; the density ratio of the sulfonic acid group to the oxygen-containing group is controlled to be from (1 to 1.25) to (1 to 8.33). The catalyst has high hydrolysis efficiency and a preparation technology is simple and is suitable for large-scale production.

The invention discloses a magnetic solid acid catalyst which uses Fe3O4 as a magnetic core. Tetraethoxysilane and N-[3-(trimethoxysilyl)propyl]ethylenediamine are subjected to sol-to-gel reaction in water and ethanol solution separately, the surface of the magnetic core is coated with amino silica, and a sulfonic acid group is modified by the sulfonation reaction of chlorosulfonic acid, so that magnetic solid acid is obtained. The invention proposes that the magnetic solid acid catalyst is applied to the hydrolysis of dioscorea nipponica and the like to extract saponin for the first time, theobtained magnetic solid acid has high catalytic activity and environmental friendliness, the yield of saponin is effectively improved, and remarkable environmental and economic benefits are achieved.

The invention discloses a fusion protein with antibacterial and repairing function and a production method and application thereof. A fusion gene is prepared by splicing an anthropogenic antibacterial peptide gene and a cell growth factor gene through a segment of hydrophobic polypeptide joint; and the fusion protein is prepared by adopting the expression of a prokaryocyte or yeast expression system, and is encoded by the anthropogenic antibacterial peptide, the polypeptide joint and the cell growth factor gene from an N end to a C end of the fusion protein. The invention simultaneously provides application of the fusion protein to the preparation of medicaments for treating wounds, burns / scalds, cold injury, chronic ulcer or bedsore. The invention creatively fuses the two kinds of polypeptide genes together to successfully construct a fusion protein with dual function of preventing infection and accelerating the repair of the damaged tissue, improves the safety of clinical medicament simultaneously, and prolongs the half-life period of the fusion protein.

The invention provides a ternary nanometer catalyst used for hydrolyzing ammonia borane to release hydrogen and a preparation method of the ternary nanometer catalyst. The catalyst takes a metal-organic framework material of MIL-101 as a carrier and sodium borohydride as a reducing agent, and the multiple catalyst of RuCuCo@MIL-101 is obtained by reducing precursors of ruthenium salts, copper salts and cobalt salts. The specific surface area of the multiple catalyst synthesized with the preparation method reaches up to 2868m<2> / g, the grain size is around 6.9nm, good catalytic activity is exerted by catalyzing the ammonia borane to be hydrolyzed to release the hydrogen under the room temperature, transformation frequency (TOF) is 241.2mol H2 min<1> (mol Ru)<1>, and activation energy (Ea) is 48 kJ mol<1>. The catalyst is still good in stability after circulation tests are performed for 5 times; the ternary nanometer catalyst doped with non-noble metal has the advantages of even distribution of the metal particles, large specific surface area, more in catalytic active sites and the like, and compared with a conventional noble-metal catalyst, the ternary nanometer catalyst is low in cost, simple in preparation, easy to obtain raw materials, suitable for industrial production and good in application prospect.

The invention provides a preparation method of a magnetic micro / nano gel-coated biological charcoal immobilized lipase. The method comprises the following steps: compounding magnetic ferroferric oxide nanoparticles and a sodium oleate solution to form a nano compound, mixing the nano compound with a temperature-sensitive monomer N-isopropyl acrylamide, a crosslinking agent and an emulsifier solution, and carrying out emulsion polymerization reaction to prepare the magnetic micro / nano gel; regulating the temperature of the solution according to the phase change characteristics of the obtained micro / nano gel, so that the micro / nano gel forms a coating on the surface of the biological charcoal; mixing the biological charcoal carrier with a free lipase solution so that the lipase molecules permeates into the micro / nano gel network of the carrier surface and the catalytic active site of the lipase is activated through the oil / water interface; and carrying out glutaraldehyde crosslinking reaction to immobilize the active lipase. The prepared magnetic immobilized lipase has higher catalytic activity, and can be conveniently separated and recovered from the reaction medium by the aid of a magnet, so the application cost is low.

The invention provides feruloyl esterase and a preparing method and application thereof. A feruloyl esterase gene coming from a soil macro gene library have the nucleotide sequence and amino acid sequence shown in SEQ ID NO.1 and SEQ ID NO.2. The gene contains a tetrapeptide SXXK sequence motif which is rarely seen, and after the esterase gene is inserted into plasmid pET28a(+), the gene is transformed into escherichia coli BL21(DE3) to achieve heterogeneous expression. The molecular weight of purified recombinase (DLFae4) is 38.3 kDa. Besides, it is put forward for the first time that novel feruloyl esterase can hydrolyze penbritin, penicillin, cefazolin and other lactam antibiotics. As is shown by site-directed mutagenesis experiments, a catalysis triplet of DLFae4 is composed of serine(S11), histidine (H74) and aspartic acid (D302), and the mutation of any of serine (S11), histidine (H74) and aspartic acid (D302) can cause loss of the catalysis capability of DLFae4. DLFae4 has a high hydrolytic activity on methyl ferulate and has good heat stability. In the presence of cellulase, DLFae4 can obviously increase the amount of ferulic acid released from destarched wheat bran. Due to peculiar activities and enzymatic characteristics of novel feruloyl esterase, novel feruloyl esterase can be applied to feed, paper making, food, pharmacy and other fields.

The invention provides an enzyme-producing microorganism for hydrolyzing D,L-pantoyl lactone and application and a sifting method of the enzyme-producing microorganism. The method specifically comprises the steps of using an enzyme-producing microorganism strain to hydrolyze D,L-pantoyl lactone, then acidizing D,L-pantoyl lactone to form a converting solution, and conducting high-performance-liquid chromatography chirality online detection on the formed converting solution to quickly sift out strains which have the catalytic activity and stereoscopic selectivity on hydrolyzing D,L-pantoyl lactone. Through the directed sifting method, it is found that a bacterial strain Fusarium verticillioides CGMCC NO.14552 has an obvious and unique advantage in the aspect of hydrolyzing D,L-pantoyl lactone, the hydrolyzing conversion rate of 1-70% D,L-pantoyl lactone within 5-10 h can reach 35% or above, and the ee value of a target product D-pantoyl lactone is larger than 98%.

The present invention is characterized by that making the cysteine of 112th site of enterokinase light chain gene produce site-specific mutation, using colibacillus and microzyme to express enterokinase light chain variant, and utilizing Zn-Sepharose and STI-Sepharose affinity chromatography so as to obtain high-purity enterokinase light chain variant protein. As compared with wild enterokinase light chain said varient has higher stability and enzyme activity.

The invention discloses novel esterase, encoding gene and an application thereof in splitting (+ / -)-1-phenethyl alcohol and (+ / -)-styralyl acetate. The amino acid sequence of the esterase is shown in SEQ ID NO.2 and the nucleotide sequence is shown in SEQ ID NO.1. Asymmetric hydrolysis is carried out on the (+ / -)-styralyl acetate in an aqueous phase and (S)-styralyl acetate with the enantiomer excess value being 95% and (R)-1-phenethyl alcohol with the enantiomer excess value being more than 99% are obtained; and asymmetric transesterification is carried out on the (+ / -)-1-phenethyl alcohol in an organic phase and (R)-styralyl acetate with the enantiomer excess value being 99% and (S)-1-phenethyl alcohol with the enantiomer excess value being more than 70% are obtained. Compared with the traditional chemical splitting phase, the novel esterase, the encoding gene and the application disclosed by the invention have the advantages of mild reaction condition, no pollution and high enantiomer excess value and the obtained product can be used for synthesizing corresponding medicines after being simply purified.

The invention belongs to the technical field of deep processing for peanut protein, and specifically relates to a method for producing active peptide from peanut cake and meal by immobilized enzyme. The method comprises the steps of: immobilizing two proteases, i.e. alkaline and neutral proteases, by adopting chemically-modified magnetic chitosan micro-particles so as to implement enzymatic hydrolysis, and then centrifuging, decolorizing, desalting by nano-filtration, grading by ultrafiltration and spray-drying to obtain peanut active peptide. Compared with the traditional preparation method,the method has the characteristics of low production cost, short production period, high product yield, high product purity, mild, green and energy-saving production process and the like. In additionto excellent dissolubility, the peanut functional peptide produced by the method provided by the invention has the functions of lowering blood pressure and resisting oxidation, so the active peptide has high market development value.

The invention relates to the technical field of lipase immobilization and provides a preparation method of charcoal-base immobilized lipase coated by nanogel modified by an oleic acid molecule. The preparation method comprises the steps of carbonizing thatch biomass into a charcoal matrix, coating the surface of the charcoal matrix by virtue of the nanogel modified by the oleic acid molecule, adequately contacting the coated charcoal matrix with a lipase solution, and carrying out interface excitation, crosslinking fixation and freeze drying on the lipase, so as to obtain the immobilized lipase. The invention simultaneously provides the immobilized lipase prepared by utilizing the preparation method. The catalytic activity of the lipase prepared by utilizing the preparation method is substantially improved.

The invention discloses a COS and CS.2. hydrolyst and its application in the field of desulfurizing agent and its application which charges ammonium salt onª†-Al.2.O.3. to prepare the catalysis.

The invention provides tertiary alcohol ester hydrolase, a gene for encoding the same, a carrier containing the encoding gene, engineering bacteria and application thereof. The amino acid sequence of the tertiary alcohol ester hydrolase is shown as SEQ ID No.2, and the amino acid sequence of the encoding gene is shown as SEQ ID No.1. The tertiary alcohol ester hydrolase is high in short-carbon chain ester hydrolytic activity; and the enzymatic activity for catalyzing hydrolysis of p-nitrophenol butyrate in a Tri-HCl buffer solution with pH of 8.5 at the temperature of 50 DEG C can reach 3,010 U / mg. The esterase is high in strong organic solvent resistance stability and activity improving characteristic; and after the esterase is incubated in strong organic solvents such as normal hexane, toluene and dimethylbenzene, of which the partition coefficient Log P is greater than 3, the enzymatic activity still can be improved by 51 percent, 44 percent and 49 percent respectively. Moreover, the esterase has tertiary alcohol ester hydrolytic activity, so that the esterase can be widely used for hydrolyzing different tertiary alcohol ester substrates. The esterase, the encoding gene, the carrier, and the recombinant bacteria can be widely applied to ester catalysis of synthesis and resolution of chiral compounds related to tertiary alcohol substrates in medicine industry, food industry, chemical industry and the like.

The invention discloses yersinia pseudotuberculosis-sourced antifungal protein and a coding gene and application thereof, and belongs to the technical field of genetic engineering. The antifungal protein is protein comprising the amino acid sequence shown as SEQ ID NO: 1, or protein obtained by substituting, deleting or inserting one or several amino acid sequences in the amino acid sequence shownas SEQ ID NO: 1 and having equivalent activity. The invention further provides the coding gene, an expression vector and engineering bacteria of the antifungal protein. The invention further providesa separation and purification method of the yersinia pseudotuberculosis-sourced antifungal protein. The protein is applied to an antifungal agent and the coding gene of the protein is applied to a transformed plant. The YPK_AFP1001 antifungal gene and the protein, related by the invention, have higher and more broad-spectrum antifungal activity.

The invention relates to an extracellular serine protease crystal structure with nematophagous fungi infectivity and belongs to the fields of molecular biology and applied microbiology. The invention implements liquid cultivation with Lecanicillium psalliotae YMF1.00112 and Paecilomyces lilacinus M-14, fractional precipitation of ammonium sulphate with a fermented liquid, and dissolution of protease-containing active component with buffer solution, and then obtains raw enzyme solution. Cation exchange chromatography and molecular sieve purification of the raw enzyme solution are implemented. After purified enzyme is obtained, concentration processing is implemented so as to obtain an enzyme solution with higher concentration. Hanging drop vapor diffused crystallization of the enzyme solution with higher concentration is implemented. The crystallization conditions are filtered with a reagent kit. Enough diffraction pictures of obtained crystal are collected through diffraction on an X-ray machine. The diffraction pictures are processed with HKL2000, protein structure is analyzed and optimized with software O, ccp4 and the like. The invention discloses the extracellular serine protease crystal structure for the first time and provides foundation for improving the hydrolysis activity of protease and the infectivity of nematophagous fungi.

The invention discloses a method of producing rutile titanium dioxide by a sulfuric acid method and a preparation method of a double-effect seed crystal used. Titanium liquid is uniformly and quicklyheated up, pyrohydrolyzed and nucleated to generate the double-effect seed crystal with high hydrolysis activity and low temperature crystal transformation ability; the seed crystal, as an additionalseed crystal, is used for a hydrolysis reaction of the industrial titanium liquid to induce the hydrolysis reaction of the titanium liquid, is high in hydrolysis activity and can accelerate the hydrolysis reaction; a metatitanic acid product with uniform particle size distribution and moderate particle size is obtained; further, the metatitanic acid product is applied to a calcining section; and metatitanic acid can be quickly converted into a high-quality rutile titanium dioxide product at a low temperature after dehydration and desulfuration without adding a rutile calcining seed crystal additionally.

The invention relates to a filling method for sulfur recycled catalyst with a high content of CO2 in a raw material gas to be treated, which belongs to the method of preparing sulfur from a sulfur compound in a gaseous state of sulfides in the gaseous state. By the adoption of a two-stage sulfur preparation and conversion process, multi-functional sulfur recycled catalyst with high hydrolysis activity of organic sulfur is filled at the height from one thirds to a half in the upper part of the first-stage converter, and alumina base sulfur recycled catalyst with a large ratio surface is filled at the height from two thirds to a half in the lower part of the first-stage converter; the second-stage converter is totally filled with alumina base sulfur recycled catalyst with the large ratio surface. According to the filling method for sulfur recycled catalyst with the high content of CO2 in the raw material gas to be treated, the hydrolysis activity of sulfur is increased, and the Claus activity can be maintained high, the gas volume of CO2 in the raw material gas to be treated is as high as 50 percent, the total sulfur conversion rate of the sulfur recycle device can reach above 96 percent, and the service life of the catalyst can reach more than six years. The filling method provided by the invention is suitable for the sulfur recycling devices of facilities in petroleum refining and production, coal chemical industry and natural gas purifying.

The invention relates to the technical field of titanium catalysts, and discloses a preparation method of a high-dispersity titanium catalyst for polyester synthesis. The preparation method comprises the following steps: adding water, a titanate compound and an organic guanidine compound into a solvent system formed by organic alcohol to form a reaction system, and carrying out a titanate hydrolysis reaction to obtain the high-dispersity titanium catalyst. The titanium catalyst prepared by the invention can be directly used for polyester synthesis, and agglomeration in the process of transferring water to an organic solvent can be avoided, so the prepared polyester has better hue; and moreover, the organic guanidine compound is adopted as the catalyst, and silica gel is added into a reaction system, so the situation that the titanium catalyst does not need to be agglomerated and is too large in size in the preparation process can be avoided, the prepared titanium catalyst has relatively high catalytic activity, and polyester prepared from the titanium catalyst is relatively good in hue.

The invention discloses an aspergillus oryzae lipase mutant and application thereof in disassembling (R,S)-2-(4-hydroxyphenoxy)propionate for preparing (R)-2-(4-hydroxyphenoxy)propionate. The mutant is obtained by site-saturated single or multiple mutations of the 38th site or the 230th site of the amino acid sequence in SEQ ID NO.1. By comparing the hydrolysis capability of mutant enzymes AOL-3F38N and AOL-3F38N-V230R and the non-mutated enzyme AOL-3, a user finds that the hydrolytic activity of the mutant enzymes AOL-3F38N and AOL-3F38N-V230R is 3.4 times and 4.0 times that of the non-mutated lipase, the thermal stability of the modified lipase is better after 50 DEG C, and the yield of the (R)-2-(4-hydroxyphenoxy)propionate is increased. The mutant is more suitable for industrial production.

The invention provides tertiary alcohol ester hydrolase, a gene for encoding the same, a carrier containing the encoding gene, engineering bacteria and application thereof. The amino acid sequence of the tertiary alcohol ester hydrolase is shown as SEQ ID No.2, and the amino acid sequence of the encoding gene is shown as SEQ ID No.1. The tertiary alcohol ester hydrolase is high in short-carbon chain ester hydrolytic activity; and the enzymatic activity for catalyzing hydrolysis of p-nitrophenol butyrate in a Tri-HCl buffer solution with pH of 8.5 at the temperature of 50 DEG C can reach 3,010 U / mg. The esterase is high in strong organic solvent resistance stability and activity improving characteristic; and after the esterase is incubated in strong organic solvents such as normal hexane, toluene and dimethylbenzene, of which the partition coefficient Log P is greater than 3, the enzymatic activity still can be improved by 51 percent, 44 percent and 49 percent respectively. Moreover, the esterase has tertiary alcohol ester hydrolytic activity, so that the esterase can be widely used for hydrolyzing different tertiary alcohol ester substrates. The esterase, the encoding gene, the carrier, and the recombinant bacteria can be widely applied to ester catalysis of synthesis and resolution of chiral compounds related to tertiary alcohol substrates in medicine industry, food industry, chemical industry and the like.

The invention discloses an environment-friendly separation process for valuable components in biomass materials. The environment-friendly separation process comprises the following steps of crushing the biomass materials; adding water for pulverizing-leaching treatment on the crushed biomass materials; activating the pulverized and leached materials in a stirring device; conveying the activated materials to a reaction kettle for pyrohydrolysis, wherein the temperature is controlled to 150-220 DEG C and the pressure is controlled to 0.5-3MPa during pyrohydrolysis; filtering a reaction product after pyrohydrolysis, wherein filter liquor mainly comprises hydrolysis components of hemicellulose in the biomass materials, filter residue can be subsequently treated, a lignin degradation component, cellulose and other components can be further separated through subsequent oxydative degradation. The method has the advantages of convenient operation, low equipment investment, low-price and easily obtained materials, environment friendliness, circular using of biomass resource and the like.