Tertiary alcohol ester hydrolase, encoding gene, carrier, engineering bacteria and application thereof

Abstract

The invention provides tertiary alcohol ester hydrolase, a gene for encoding the same, a carrier containing the encoding gene, engineering bacteria and application thereof. The amino acid sequence of the tertiary alcohol ester hydrolase is shown as SEQ ID No.2, and the amino acid sequence of the encoding gene is shown as SEQ ID No.1. The tertiary alcohol ester hydrolase is high in short-carbon chain ester hydrolytic activity; and the enzymatic activity for catalyzing hydrolysis of p-nitrophenol butyrate in a Tri-HCl buffer solution with pH of 8.5 at the temperature of 50 DEG C can reach 3,010 U / mg. The esterase is high in strong organic solvent resistance stability and activity improving characteristic; and after the esterase is incubated in strong organic solvents such as normal hexane, toluene and dimethylbenzene, of which the partition coefficient Log P is greater than 3, the enzymatic activity still can be improved by 51 percent, 44 percent and 49 percent respectively. Moreover, the esterase has tertiary alcohol ester hydrolytic activity, so that the esterase can be widely used for hydrolyzing different tertiary alcohol ester substrates. The esterase, the encoding gene, the carrier, and the recombinant bacteria can be widely applied to ester catalysis of synthesis and resolution of chiral compounds related to tertiary alcohol substrates in medicine industry, food industry, chemical industry and the like.

Description

(1) Technical field

[0001] The invention relates to a tertiary alcohol ester hydrolase, a gene encoding the enzyme, a carrier containing the encoding gene, engineering bacteria and application thereof. (2) Background technology

[0002] Esterase is an enzyme that can hydrolyze fatty acid esters to produce free fatty acids, hydrolyze oils into fatty acids and glycerol, and also catalyze ester synthesis and transesterification. As an important class of biocatalysts, esterases are widely used in light industry, chemical industry, medicine, food and other industries because of their high selectivity, mild catalytic reaction conditions, and no pollution. In recent years, with the deepening of non-aqueous enzymology and interfacial enzymology, the application of esterase has also been continuously expanded, and it is widely used in ester synthesis, resolution of chiral compounds, selective group protection of chemical synthesis intermediates, Polymer synthesis, peptide synthesis,...

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