The invention discloses a method for extracting
rapeseed peptides through
wet milling combined with an enzymic method. The method comprises the following steps of: taking dry degreased and unshelled
rapeseed dregs,
grinding and filtering the dry degreased and unshelled
rapeseed dregs through a 40-mesh
sieve, adding water which is 5 times of the materials in weight, adding 5-10 u / g of
cellulase, and performing
wet grinding on black
soybean meal for 1-3 hours under the condition of a rotating speed in the range from 250 to 400 rpm (
revolutions per minute) by using a
ball mill to obtain an ultrathin
powder turbid liquid; (2) supplementing water to the ultrathin
powder turbid liquid until the concentration of the dry materials is in the range from 50 to 100 g / l, adjusting the pH to 7.0, adding 40u / g of
protease, maintaining the temperature at 55 DEG C and the pH at 7.0, and hydrolyzing for 1-2 hours; (3) centrifuging the enzymatic
hydrolysate for 15 minutes under the condition of 10000 rpm, continuously adding water which is 5-10 times of residues in weight to the residues, adding 20u / g of
protease, hydrolyzing for 1-2 hours at the temperature of 55 DEG C and the pH of 7.0, centrifuging, and blending the two supernates to obtain a rough
peptide solution. According to the invention,
cellulose dissolving causes large-
molecular materials such as
protein to be hydrolyzed more easily, and also the
hydrolysis rate of the
protein can be increased; in addition, the conversion rate of the peptides is greatly increased.