Method for extracting rapeseed peptides through wet milling combined with enzymic method

Abstract

The invention discloses a method for extracting rapeseed peptides through wet milling combined with an enzymic method. The method comprises the following steps of: taking dry degreased and unshelled rapeseed dregs, grinding and filtering the dry degreased and unshelled rapeseed dregs through a 40-mesh sieve, adding water which is 5 times of the materials in weight, adding 5-10 u / g of cellulase, and performing wet grinding on black soybean meal for 1-3 hours under the condition of a rotating speed in the range from 250 to 400 rpm (revolutions per minute) by using a ball mill to obtain an ultrathin powder turbid liquid; (2) supplementing water to the ultrathin powder turbid liquid until the concentration of the dry materials is in the range from 50 to 100 g / l, adjusting the pH to 7.0, adding 40u / g of protease, maintaining the temperature at 55 DEG C and the pH at 7.0, and hydrolyzing for 1-2 hours; (3) centrifuging the enzymatic hydrolysate for 15 minutes under the condition of 10000 rpm, continuously adding water which is 5-10 times of residues in weight to the residues, adding 20u / g of protease, hydrolyzing for 1-2 hours at the temperature of 55 DEG C and the pH of 7.0, centrifuging, and blending the two supernates to obtain a rough peptide solution. According to the invention, cellulose dissolving causes large-molecular materials such as protein to be hydrolyzed more easily, and also the hydrolysis rate of the protein can be increased; in addition, the conversion rate of the peptides is greatly increased.

Description

technical field [0001] The invention belongs to the technical field of food processing, and relates to a method for extracting rapeseed polypeptide by wet ball milling combined with an enzymatic method. Background technique [0002] Rapeseed is one of the important oil crops, and the output of rapeseed in my country ranks first in the world. Under normal circumstances, rapeseed can be processed to obtain about 3506 rapeseed oil and about 65% rapeseed meal. Rapeseed meal is rich in crude protein, crude fat, lecithin, multivitamins, amino acids, etc. The digestible protein content of its protein can reach 27.8%. In addition, rapeseed meal also contains high levels of choline, biotin, folic acid, niacin, thiamine, riboflavin and other B vitamins and various trace amounts of Ca, P, Fe, Se, Cu, Zn, Mn, etc. element. The crude protein content of rapeseed meal is generally 35%-45%. It is a high-quality full-price vegetable protein, and its development and utilization have import...

AI Technical Summary

Problems solved by technology

At present, in the enzymatic hydrolysis process, the rapeseed cake is mainly crushed and hydrolyzed directly. In this process, most of the rapeseed protein is insoluble in water, and the enzymatic hydrolysis efficiency is low.