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Preparation method of novel protein feed additive

A protein feed and additive technology is applied in the preparation of new protein feed additives, the preparation of hydrolyzed protein mixtures and protein peptide powder feed additives, and can solve the problem of protein degradation and anti-nutritional factors. , can not take into account the optimal reaction conditions and other issues, to achieve the effect of improving apparent digestibility, reducing piglet feed-to-meat ratio, and improving transformation utilization rate

Active Publication Date: 2009-09-16
BEIJING DABEINONG TECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Enzyme method has a wide range of uses in the food industry, but most of them use a single protease, which has limited degradation of protein and the destruction of anti-nutritional factors, so it is difficult to be applied in practice
[0005] Patent CN1530022 discloses a preparation method of protein feed, which adopts biological fermentation method combined with enzyme method to prepare, on the one hand, fermentation removes anti-nutritional factors, and on the other hand, protein is rapidly degraded by enzyme; however, considering the growth cycle of bacteria , the fermentation time is still long; and adding bacteria and enzymes at the same time cannot take into account the optimal reaction conditions of the two; in addition, the use of solid fermentation method, the product quality is not stable
Patents CN1377590 and CN1274361 disclose methods for producing soybean peptides by liquid enzymatic methods, both of which use a single protease to hydrolyze proteins only, and do not degrade non-starch polysaccharides in vegetable proteins
Patent CN1957736 discloses a production method of soybean protein (peptide) powder, which involves a comprehensive development and utilization of soybean meal, but due to its cumbersome process, it is not conducive to the application and promotion of low value-added feed industry

Method used

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  • Preparation method of novel protein feed additive
  • Preparation method of novel protein feed additive
  • Preparation method of novel protein feed additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Hydrolyzed protein mixture—H1

[0050] 1) Raw material pretreatment:

[0051]The enzymolysis substrate - soybean protein isolate is pulverized to 100 mesh by a pulverizer, put into the enzymolysis tank, add water, the weight ratio of the weight of the added water to the protein contained in the enzymolysis substrate is 100:5, Stir evenly, adjust the pH to 7, soak in an enzymatic hydrolysis tank at 30°C for 1 hour, then sterilize at 80°C for 20 minutes, cool the sterilization solution to 50°C, and prepare for enzymatic hydrolysis;

[0052] 2) Enzymatic hydrolysis treatment of compound enzyme:

[0053] To the pretreated raw material obtained in step 1), sequentially add non-starch polysaccharide enzyme (NSP enzyme) and protease for hydrolysis. During the reaction, the temperature is 45° C., the pH is natural, and the enzymolysis is carried out for 14 hours until the degree of hydrolysis (DH%) is 20%. stop reacting when

[0054] The amount of various enzymes a...

Embodiment 2

[0075] Example 2 Hydrolyzed protein mixture—H2

[0076] 1) Raw material pretreatment:

[0077] The enzymatic hydrolysis substrate - soybean meal is crushed to 100 mesh by a pulverizer, put into the enzymatic hydrolysis tank, add water, the ratio of the weight of the added water to the weight of the protein contained in the enzymatic hydrolysis substrate is 100:16, and stir evenly , adjust the pH to 7, soak in the enzymatic hydrolysis tank at 37°C for 0.5 hours, then perform 10-minute sterilization at 100°C, cool the sterilizing solution to 60°C, and prepare for enzymatic hydrolysis;

[0078] 2) Enzymatic hydrolysis treatment of compound enzyme:

[0079] To the pretreated raw material obtained in step 1), sequentially add non-starch polysaccharide enzyme (NSP enzyme) and protease for hydrolysis. During the reaction, the temperature is 45° C., the pH is natural, and the enzymolysis is carried out for 24 hours until the degree of hydrolysis (DH%) is 42%. stop reacting when

[...

Embodiment 3

[0087] Example 3 Hydrolyzed Protein Mixture H2 Reducing Sugar Release Amount

[0088] (1) Determination of reducing sugar

[0089] ① Drawing of standard curve

[0090] Take 40MI of acetic acid-sodium acetate buffer solution (pH5.5), add 5.0ml of DNS reagent, heat in boiling water for 5min, cool to room temperature with tap water, and dilute to 25ml with water to make a standard blank solution.

[0091] Pipette 1.00ml, 2.00ml, 3.00ml, 4.00ml, 5.00ml, 6.00ml, 7.00ml of glucose solution respectively, and dilute to 100ml with buffer respectively, and prepare glucose standard solution with concentration of 0.10mg / ml~0.70mg / ml .

[0092] Draw 2.00ml of the glucose standard solutions of the above concentration series (make two parallels), add them to the graduated test tubes respectively, then add 2ml of water and 5ml of DNS reagent respectively, oscillate electromagnetically for 3s, heat in boiling water for 5min, and then. Cool to room temperature with tap water, then dilute to ...

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Abstract

The invention relates to a preparation method of a hydrolyzed protein mixture and a protein peptide powder feed additive, wherein a multienzyme complex enzyme method is used for hydrolyzing vegetable protein to prepare the hydrolyzed protein mixture and protein peptide powder. The technology of the method is simple, and a non-starch polysaccharide enzyme and a proteinase are compounded so as to be capable of improving the hydrolyzation rate and degree of protein as well as the utilization rate of feed and reducing the production cost. Products prepared by the preparation method can be used as the feed additive to be capable of significantly improving the laying rate of egg-laying poultry, improving the weight increment of meat poultry, reducing the feed-meat rate and improving the production performance of table poultry as well as reducing the incidence rate of diarrhea in young animals and the feed-meat rate of piggy; and the preparation method is applied to aquatic livestock to be capable of improving the weight increment of fish and reducing the bait coefficient and content viscosity in the intestinal tract.

Description

technical field [0001] The invention belongs to the field of feed additives, and relates to a preparation method of a novel protein feed additive, in particular to a preparation method of a hydrolyzed protein mixture and protein peptide powder feed additive. Background technique [0002] Insufficient feed resources, especially the shortage of protein resources in feed, are the main problems restricting the development of my country's aquaculture industry. Moreover, in the utilization of protein feed resources, my country still has the problem of low conversion rate and utilization rate. Therefore, the development and reasonable The use of protein feed is of great significance to the development of aquaculture. [0003] Studies have found that existing protein feeds often have anti-nutritional factors and toxicity problems, which affect the safety of use; on the other hand, most of the digested protein in the feed is absorbed by oligopeptides rather than monomeric amino acids....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K20/147A23K40/00
Inventor 赵雁青张玳华姚琨贾秋英白玉卿孙占敏刘宇赵军
Owner BEIJING DABEINONG TECH GRP CO LTD
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