Page 1 of 3 pages. Details of the invention? A name that reflects the invention? A method for increasing
biomass digestion enzyme production by culturing different fungal strains together under specific conditions.
Solid fermentation followed by liquid
fermentation. Five branches of science related to invention.
Biotechnology related to methods for increasing the production of
biomass digesting enzymes through... Cultivating different strains of fungi together under
solid fermentation conditions followed by liquid fermentation. Background in the relevant art or science? Enzymes that digest lignocellulose
biomass, namely
cellulase and
xylanase, are used. It is widely used in various industries worldwide, such as food,
animal feed, textiles, and
agriculture. The demand for
cellulase and
xylanase enzymes accounts for 20% of the global
enzyme market.
Cellulase enzymes consist of three groups: endoglucanase, exoglucanase, and β-.
Glucosidase is a group of enzymes that catalyze the breakdown of (1-4)-glycosidic bonds. Within the molecular structure of
cellulose, if
digestion is complete, glucose is obtained, while the
enzyme cytokinesis... 15. Lanase consists of two groups of enzymes: endoxylanase and α-xylosidase, which are enzymes that digest...
Hemicellulose is broken down into its smallest units, which are
xylose sugars.
Biomass digestive enzymes can be produced by culturing fungi through
solid fermentation or fermentation. Liquid fermentation versus
solid fermentation has the
advantage of providing conditions for
fungal growth on the biomass surface. The solids, which serve as both nutrients and binding sites, use minimal water and energy; however, fermentation... 20. Liquids also have the
advantage of
mass transfer in the
liquid phase, combined with stirring and mixing, resulting in
mass transfer. Fungal cells require adequate nutrients and
oxygen for culturing different fungal strains together. One approach to compensating for
genome variation between strains of microorganisms is to increase the expression of... Enzymes working together create a synergistic effect in biomass
decomposition to produce sugars for growth. And it stimulates the production of enzymes in both strains, including the application of the obtained enzymes in production. 25. Other
biological substances next. Although there have been some reports of biomass
digestion enzyme production using different fungal strains, there is still no... Cultivating different strains of fungi together under a two-stage fermentation process, in which the fungi are cultured. The potential for different catalytic reactions will result in a diverse group of enzymes that help to enhance... The total output of enzyme production and the products from the action of this group of enzymes, in addition to... 30. The two-stage fermentation process also promotes the synergistic action of fungi under both solid fermentation and liquid fermentation conditions. And liquids, therefore, a method to increase biomass digestion enzyme production by culturing different strains of fungi together? Under conditions of solid fermentation followed by liquid fermentation, a process was developed for
batch production. Enzymes that work together efficiently lead to cost reduction, improved quality, and enhanced efficiency. Production of
bioproducts from lignocellulose biomass. 35. From searching patent and utility model databases in both Thailand and abroad, the following patents were found: A utility model patent that is similar to this invention is: Korean patent number KR20120082123A describes a mixed enzyme preparation for... Degradation of non-
starch polysaccharides by the fungi
Aspergillus oryzae GB-N1 and
Aspergillus niger GB-X2. Page 2 of 3. By culturing under forced-circulation solid fermentation conditions with controlled
moisture content. During fermentation, however, there are limitations as it does not specify the relationship between the two fungal strains to production.
Enzyme mixture? Australian Patent No. AU2013299022B2 presents a biomass conditioning method. 5. Lignocellulose is broken down by heating and water under pressure, followed by
enzymatic digestion to produce
sugar. Xilostat? This patent does not present a method for increasing the production of mixed enzymes. U.S. Patent No. WO2015014364A1 proposes a method for conditioning lignum biomass.
Cellulose is broken down using acids and bases combined with heating, followed by
enzymatic digestion to produce
sugar.
Xylose and
arabinose? This patent does not present a method for increasing the production of the enzyme mixture. 10 Characteristics and Purposes of Invention This invention involves a process for increasing biomass digestion enzyme production by culturing different fungi. The genetic strains are combined under solid fermentation conditions followed by liquid fermentation in the following steps: The preparation of fungal inoculum with different enzymatic activity compared to the preparation of lignocellulose biomass
waste material. From
agriculture or industry, solid fermentation is followed by liquid fermentation and
processing. 15. Pure enzyme? (partially) This invention aims to increase biomass digestion enzyme production by culturing different fungal strains. The combination of genetic material under solid fermentation followed by liquid fermentation can stimulate... The production of enzyme blends is lower than traditional methods, and the resulting enzyme blends are highly efficient in
biodegradation.
Cellulose and
hemicellulose masses. 20. Complete disclosure of the invention. This invention proposes a method for increasing biomass digestion enzyme production by culturing different fungal strains. The genetic material is combined under solid fermentation conditions followed by liquid fermentation in the following steps: a. Prepare two or more fungal inoculum strains with different enzyme activities by culturing them on...
Agar-
agar dish consisting of 20% potatoes, 2% dextrose
sugar, and 1.5%
agar-
agar powder. pH. 255.0-5.5 After 7-10 days, transfer the resulting spores to a
liquid culture medium containing
yeast extract. 0.1% and a surfactant such as Tween-80 at 0.1%. In this step, a fungal inoculum is obtained.
Enzyme activity? It's different. B. Prepare
lignocellulosic biomass, which is agricultural or
industrial waste material, such as: Rice
bran, corn
husk, palm
bunches, coconut
husk, etc., are dried in the sun for a period of time. 302-3 days in this stage, resulting in dried
lignocellulosic biomass? C. Perform solid fermentation by spraying a liquid fungal culture medium containing spores of both fungi. 911 The biomass strains from step B should produce 10-10 spores per strain. Mix 60-70%
moisture content biomass (1.5-2.3 liters per
kilogram of biomass) thoroughly and ferment in... A rectangular container with a biomass layer height not exceeding 5 centimeters should be covered with cheesecloth and sprayed with
disinfectant. Add 0.15-0.23 liters of water and mix well daily. Ferment at
room temperature for 2-3 days. This step is complete. Fungi that grow on biomass. d. Perform liquid fermentation by transferring the solid-state biomass fermented from step b into a
mixing tank. Mix and add 40-50 liters of liquid feed per
kilogram of biomass, stirring at a speed of 100
revolutions per minute. (Page 3 of 3) Liquid fermentation takes 5-7 days. This stage yields a fermented liquid with high mixed enzyme activity and biomass. Leftover from fungal fermentation. Step J: Filter and separate the fermented biomass from the fermentation liquid. Then, separate the fine
sediment from the resulting fermentation liquid. Using a
centrifuge for 20-30 minutes, settle the
clear liquid with
acetone at an
acetone to particle ratio. 5 parts clear to 2 parts to 1 by volume. The resulting
sediment is partially purified biomass enzyme that can... Can it be reused or benefited from further? What is the best way to invent something? As already mentioned in the section on complete disclosure of the invention;