Bacilli for efficiently degrading soybean meal antigen protein and bacterium enzyme mixed fermentation method

Abstract

The invention discloses bacilli for efficiently degrading soybean meal antigen protein and a bacterium enzyme mixed fermentation method. A group of bacilli for efficiently degrading soybean meal antigen protein comprise two strains of bacilli, belong to Bacillus subtilis subsp.subtilis, are preserved in CGMCC (China General Microbiological Culture Collection Center), and respectively have the collection number of CGMCC NO:12823 and CGMCC NO:12824. A method for degrading feed anti-nutrition factors through bacterium enzyme mixed fermentation comprises the following steps of preparing corn, bean meal and bran into composite feed; adding bacillus fermentation seed liquid accounting for 3 to 5 percent of the total mass; adding non-starch polysaccharide composite enzyme liquid accounting for 0.5 to 1 percent of the total mass; performing mixing and uniform stirring; performing fermentation for 24 hours at 30 to 40 DEG C to obtain fermentation mixed feed; adding 5 percent of lactobacillus seed liquid into the fermentation mixed feed; after the uniform mixing, encapsulating the materials into an anaerobic fermentation device; after the fermentation for 48 hours, obtaining finished product feed. The bacilli have the advantages that the anti-nutrition factor degradation effect is obvious; the fermentation acid flavor is realized; the food calling effect is good; good production and application prospects are realized.

Description

technical field [0001] The invention relates to a method for degrading feed anti-nutritional factors through bacterial enzyme synergistic fermentation, in particular to the degradation of anti-nutritive factors in corn and soybean meal-based diets. Background technique [0002] Corn soybean meal bran is currently the main feed raw material, and raw soybeans contain a large number of anti-nutritional factors, such as trypsin inhibitors, lectins, antigenic proteins, phytic acid and non-starch polysaccharides. The main antigenic proteins are macroglobulin and β-conglobulin. The bran contained in the diet also contains more non-starch polysaccharides. It is not conducive to the digestion and absorption of animals, especially young animals. [0003] Traditional physical processing methods such as heating and puffing can destroy a large number of heat-labile anti-nutritional factors such as lectins. However, the degradation of antigenic proteins, phytic acid, and non-starch pol...

AI Technical Summary

Problems solved by technology

[0004] However, the current bio-fermented feed mainly focuses on the fermentation of soybean meal, and the fermentation of compound feed is rare

Images