34results about How to "Prospects for production and application are good" patented technology

The invention relates to a Gaussian process based injection molding forming process robust design and tolerance design method. The Gaussian process based injection molding forming process robust design and tolerance design method comprises obtaining the correlation of process parameters and quality objectives by a Gaussian process based self-adaptive canonical correlation analysis method and classify the parameters into main parameters and secondary parameters according to the sizes of degrees of the correlation; establishing a robust optimization model of the main parameters, adding the fluctuation quantity into an objective function and constraint conditions and obtaining a robust process parameter combination through simultaneous optimization of the two targets of the mean and variance to reduce the complexity of calculation of a multi-parameter model reasonably; performing analysis on the relation of the process parameter fluctuation tolerance range and the cost, establishing a mathematical expectation model of a quality overall loss and setting a reasonable tolerance range for every process parameter. According to the Gaussian process based injection molding forming process robust design and tolerance design method, the influence of human experience and trial and error experiment is greatly reduced in the design process, the design cycle is short, the qualified rate of products is high, the system cost is low, an intelligent control project is implemented conveniently, and the Gaussian process based injection molding forming process robust design and tolerance design method can be widely applied to the forming process of various materials.

The invention discloses a method for modifying a cellulose acetate filtering stick for a cigarette by adopting high-power electron beams. The method is characterized by comprising the following steps: (1) carrying out transmission irradiation on the cellulose acetate filtering stick in a nitrogen gas or air atmosphere by using the high-power electron beams generated by a high-power electron accelerator or a cobalt source, and leaving the filtering stick to stand after irradiation is finished, wherein the irradiation dose is controlled to be within a range of 50-100 KGy; (2) placing the filtering stick subjected to irradiation modification obtained in the step (1) for 10h under vacuum negative pressure condition with temperature of 40-60 DEG C for removing macromolecules such as free acetic acid or neutralizing acidic components, and drying the filtering stick; and (3) balancing water content in the neutralized or separated filtering stick obtained in the step (2), and directly applying the filtering stick to the split rolling technology for preparing the cigarette.

The present invention relates to a kind of chitinase cm Chi6 gene and its clone expression and application. The present invention clones for the first time the exochitinase with the nucleotide sequence shown in SEQ ID No.1 cm Chi6 gene, and obtained high-activity exochitinase, the preparation method is simple, the production cost is low, the product is easy to store, the suitable enzymatic hydrolysis temperature is 45°C, the optimum pH of enzymolysis is 7.4, at 25-50°C Good thermostability, 33 hours still maintain more than 60% of the enzyme activity; optimum pH 7.4, at pH 7.0‑10.0, 33 hours of enzyme activity still maintain more than 75%; Ba 2+ right cm Chi6 activity has a slight activation effect; cm Chi6 has high degradative activity on colloidal chitin, which can be used to prepare chitobiose with high conversion rate, low energy consumption, and environmental friendliness. It has important economic value in the application of industrial chitin degradation.

The invention discloses a metarhizium anisopliae transgenic strain as well as a preparation method and an application thereof. The strain is a recombinant engineering bacterium constructed by fusion of metarhizium anisopliae genes and exogenous Prla (subtilisin-like protease A) genes. The metarhizium anisopliae transgenic strain takes metarhizium anisopliae as the basis, takes Pgpd as a strong promoter, takes TTrpC as a terminator and takes Basta as a selection marker, a Prla overexpression box is constructed with a PCR fusion method, then a fungal protoplast is prepared, genetic engineering transformation is conducted with a CaCl2-PEG method, and the overexpression box is inserted in a metarhizium anisopliae gene group randomly for overexpression. The metarhizium anisopliae transgenic strain is good in German cockroach killing effect, higher in toxicity and capable of effectively controlling German cockroach populations in a short time.

The invention relates to canine influenza virus monoclonal antibody hybridoma cell strain F112 and belongs to the technical field of biology. Immune antigens are prepared by using canine influenza virus epidemic strain A / Canine / Nanjing / 11 / 2012 (H3N2) through lymphocyte hybridoma technique, the efficient hybridoma cell strain F112 is screened out from the antigens, and the canine influenza virus monoclonal antibody hybridoma cell strain F112 has hemagglutination inhibition titer of 212 and neutralizing titer of 105 for canine influenza virus. A canine influenza preventive therapeutic agent prepared by using F112 monoclonal antibody is used for preventing and treating canine influenza, under the effective rate of 100%. The efficient F112 monoclonal antibody is applicable to the detection and diagnosis of canine influenza and the preventive therapy, and has a promising application prospect.