34results about How to "Prospects for production and application are good" patented technology

The invention discloses bacilli for efficiently degrading soybean meal antigen protein and a bacterium enzyme mixed fermentation method. A group of bacilli for efficiently degrading soybean meal antigen protein comprise two strains of bacilli, belong to Bacillus subtilis subsp.subtilis, are preserved in CGMCC (China General Microbiological Culture Collection Center), and respectively have the collection number of CGMCC NO:12823 and CGMCC NO:12824. A method for degrading feed anti-nutrition factors through bacterium enzyme mixed fermentation comprises the following steps of preparing corn, bean meal and bran into composite feed; adding bacillus fermentation seed liquid accounting for 3 to 5 percent of the total mass; adding non-starch polysaccharide composite enzyme liquid accounting for 0.5 to 1 percent of the total mass; performing mixing and uniform stirring; performing fermentation for 24 hours at 30 to 40 DEG C to obtain fermentation mixed feed; adding 5 percent of lactobacillus seed liquid into the fermentation mixed feed; after the uniform mixing, encapsulating the materials into an anaerobic fermentation device; after the fermentation for 48 hours, obtaining finished product feed. The bacilli have the advantages that the anti-nutrition factor degradation effect is obvious; the fermentation acid flavor is realized; the food calling effect is good; good production and application prospects are realized.

The invention discloses a special seed dressing agent with effects of increasing the survival rate and invigorating seedlings for sweet corn. The seed dressing agent comprises gamma-aminobutyric acid, matrine, thiamethoxam, sedaxane, carboxin, amino-oligosaccharin, salicylic acid, glycine betaine, choline chloride, fulvic acid, starch grafted potassium acrylate, calcium chloride, a wetting dispersant, an emulsifier, gibberellin and microelement water soluble fertilizer mother liquor. Under normal conditions or adverse conditions of drought, low temperature and the like, the seed dressing agent has an obvious effect of improving the seed vigor and seedling quality of the sweet corn, can be used for effectively increasing the yield of the fresh spike of the sweet corn, also has effects of exterminating underground insects, protecting and invigorating seedlings and preventing and treating main plant diseases and insect pests of the corn, has control efficiency up to 70%-90% for plant diseases and insect pests in a seedling stage, has control efficiency up to 87%-96% for corn head smut, and not only can help farmers to save labor cost and seed cost greatly, but also can increase the yield by 15%-20%, thus having excellent social and economic benefits and application prospects.

The invention relates to a Gaussian process based injection molding forming process robust design and tolerance design method. The Gaussian process based injection molding forming process robust design and tolerance design method comprises obtaining the correlation of process parameters and quality objectives by a Gaussian process based self-adaptive canonical correlation analysis method and classify the parameters into main parameters and secondary parameters according to the sizes of degrees of the correlation; establishing a robust optimization model of the main parameters, adding the fluctuation quantity into an objective function and constraint conditions and obtaining a robust process parameter combination through simultaneous optimization of the two targets of the mean and variance to reduce the complexity of calculation of a multi-parameter model reasonably; performing analysis on the relation of the process parameter fluctuation tolerance range and the cost, establishing a mathematical expectation model of a quality overall loss and setting a reasonable tolerance range for every process parameter. According to the Gaussian process based injection molding forming process robust design and tolerance design method, the influence of human experience and trial and error experiment is greatly reduced in the design process, the design cycle is short, the qualified rate of products is high, the system cost is low, an intelligent control project is implemented conveniently, and the Gaussian process based injection molding forming process robust design and tolerance design method can be widely applied to the forming process of various materials.

The invention discloses a metarhizium anisopliae transgenic strain as well as a preparation method and an application thereof. The strain is a recombinant engineering bacterium constructed by fusion of metarhizium anisopliae genes and exogenous Prla (subtilisin-like protease A) genes. The metarhizium anisopliae transgenic strain takes metarhizium anisopliae as the basis, takes Pgpd as a strong promoter, takes TTrpC as a terminator and takes Basta as a selection marker, a Prla overexpression box is constructed with a PCR fusion method, then a fungal protoplast is prepared, genetic engineering transformation is conducted with a CaCl2-PEG method, and the overexpression box is inserted in a metarhizium anisopliae gene group randomly for overexpression. The metarhizium anisopliae transgenic strain is good in German cockroach killing effect, higher in toxicity and capable of effectively controlling German cockroach populations in a short time.

The invention discloses a trolley automatic traveling method during converter tapping. The trolley automatic traveling method comprises the steps: (1) a real-time image of a steel flow in the converter tapping process is collected and stored; (2) the real-time image of the steel flow is converted into a grayscale image, the converted grayscale image is subjected to image enhancement processing, and then the grayscale image subjected to image enhancement processing is binarized and denoised; (3) the characteristic boundary of the steel flow is determined from the binarized image, and the position of the real-time center line of the steel flow is obtained based on the characteristic boundary to dynamically track the steel flow; and (4) based on the real-time change of the position of the real-time center line of the steel flow, the trolley traveling amount of a trolley is output so as to enable the trolley to travel along with the change of the position of the steel flow to accurately bear molten steel. In addition, the invention further discloses a trolley automatic traveling system. The trolley automatic traveling system comprises an image collecting device, an image storing module, an image processing module, a dynamic tracking module and a control module.

The invention provides a simple method for breeding aphelinus mali. The simple method is characterized by comprising steps of (1), providing annual malus prunifolia seedlings and planting the malus prunifolia seedlings in cups or pots when 7-8 leaves grow on each malus prunifolia seedling; (2) inoculating 3-5 adult eriosoma lanigerum on the planted malus prunifolia seedlings, placing the malus prunifolia seedlings with the eriosoma lanigerum into screen cages with a large quantity of aphelinus mali which is subjected to eclosion in advance when nymphs of the eriosoma lanigerum on branches of the malus prunifolia seedlings develop into fourth-stage eriosoma lanigerum or adult eriosoma lanigerum, enabling the aphelinus mali to perform parasitism on the malus prunifolia seedlings in the screen cages for 48 hours and then taking out the malus prunifolia seedlings; continuing feeding the eriosoma lanigerum on the malus prunifolia seedlings for 1-2 days after the eriosoma lanigerum turns black, trimming the branches of the malus prunifolia seedlings by pruning shears or brushing off black pupae by brushes. The simple method has the advantage that the aphelinus mali can be quickly and efficiently bred.

The invention discloses a method for producing inulin by adopting a membrane separation process and belongs to the technical field of biological extraction and separation. The method for producing the inulin by adopting the membrane separation process comprises the following steps: carrying out three-stage filtration on an inulin extracting solution by virtue of a hollow fiber membrane, an ultrafiltration membrane and a nanofiltration membrane, and then carrying out adsorption treatment by utilizing macroporous resin, so that purity of the inulin can reach more than 99% finally, and yield also can reach more than 90%. The macroporous resin adopted in the invention is synthesized through multi-stage reaction polymerization and has the advantages of high adsorption rate and good specificity.

The invention discloses an environmental-friendly engineering plastic particle formula, and a processing method. The environmental-friendly engineering plastic particle is prepared from, by mass, 40.0 to 50.0% of nylon-6, 15.0 to 25% of nylon-66, 5 to 10% of glass microbead, and 25 to 30% of glass fiber; is prepared via steps of material selection, material mixing, material stirring, melting and filtering, cooling, extrusion and wiredrawing, particle cutting, filtering and air drying, and packaging and storing; possesses high strength, flame resistance, corrosion resistance, mechanical strength, and excellent chemical stability; and is low in cost. The processing steps are simple; full utilization of processing equipment is realized; energy consumption of processing is reduced as much as possible; resources are saved; pollution on the environment is small; production accords with state industrial policies; and production and application prospect is promising.

The invention discloses a strain for improving the intestinal tracts of aquatic animals. The strain is separated and selected from a substrate sludge sample from a culture pond of Ningde city in Fujian province, and the strain is Bacillus licheniformis FA08 which is collected in the China General Microbiological Culture Collection Center (CGMCC) with the collection number of CGMCC No: 9544 on August 25, 2014. In addition, the invention further discloses a method for preparing a probiotic preparation for improving the intestinal tracts of aquatic animals from the strain. The prepared microbial preparation has a good effect of inhibiting aquatic pathogenic bacteria such as vibrio alginolyticus and aeromonas hydrophila, can produce a variety of active enzymes, can reduce the feed coefficient of the aquatic animals and increase the survival rate of the aquatic animals and has good production and application prospect.

The invention discloses a beta-N-acetylglucosaminidase 159 as well as cloning expression and application of the beta-N-acetylglucosaminidase 159. The gene of the beta-N-acetylglucosaminidase 159 is constructed on a plasmid pET-28a (+) through a genetic engineering means and is transferred into E. coli BL21 (DE3) for expression; the molecular weight of the recombinase is 92 kDa; and according to a result of comparative analysis, the beta-N-acetylglucosaminidase 159 belongs to a glycoside hydrolase GH20 family. Research on enzymatic properties of the recombinase shows that the specific activity of a pure enzyme is 16.67 U / mg, an optimum temperature is 40 DEG C, an optimum pH value is 9.0, the enzyme activity can be maintained to be higher than 80% under the condition that the pH value is higher than 7, and the beta-N-acetylglucosaminidase 159 shows relatively high pH stability and synergistic effect when the recombinase is in synergism with other chitinase; and thus, a theoretical basis is provided for efficient preparation of N-acetylglucosamine.

The invention discloses a method for rapidly improving tenderness of fish meat of fugu. The method comprises the following steps of: cutting the fish meat of the fugu into blocks; immersing the blocksinto a papain tenderizing solution; sealing and packaging; and carrying out ultrasonic treatment to obtain tenderized fish meat. According to the method disclosed by the invention, an ultrasonic waveand papain tenderizing technology is cooperated to reduce the acting temperature and time of papain, and flavor substance changes, nutrient loss and freshness reduction, caused by continuous high temperature, are avoided; the fish meat is tenderized for a short time and the specific flavor, freshness and nutrient value of the fish meat of the fugu are kept; a tenderizing method adopted by the invention has high production efficiency and low energy consumption; efficient tenderization of the fish meat of the fugu and the keeping of flavor nutrients and freshness can be realized; and the methodhas a good production and application prospect.

The invention relates to the technical field of arts and crafts, and discloses a color protection method for dried blue cornflower. A blue cornflower material is pretreated and then immersed with a prepared color fixative. By changing osmotic equilibrium of the cell liquid in the petal, cytochrome enzyme activity is inhibited, and a group of the same color scheme is introduced into the pigment. The content of organic salt in the prepared color fixative is high, and through a reduction reaction and complex reaction with the cornflower pigment molecules, a stable blue substance is generated. After the color protection treatment, chromaticiy is almost the same as chromaticiy of the original flower color and the color does not fade for a long time. By the chemical color protection technology,the problem that the flower material is easy to fade in the process of blue cornflower production is solved, and the dried flower product reaches the great ornamental and long-lasting effects. The method of the invention is simple and fast, is easy to operate, is low-cost, has a good production application prospect, can help achieve sustainable development and utilization of cornflower resources,and is a technical scheme worthy of promotion and application.

The modular robot comprises a machine body plate, a closed machine shell, four bionic elephant legs, a bionic elephant nose paint spraying mechanism, a paint spraying power assembly used for driving the bionic elephant nose paint spraying mechanism to move, a paint spraying control assembly electrically connected with the paint spraying power assembly and the bionic elephant legs, and a bionic elephant nose rust removing mechanism. The rust removal control assembly is electrically connected with the bionic elephant nose rust removal mechanism and the bionic elephant legs; the laser radar is arranged at the front end of the closed machine shell and electrically connected with the paint spraying control assembly and the rust removal control assembly; a temperature sensor, a first visual sensor and a paint coating thickness measuring sensor which are electrically connected with the paint spraying control assembly are arranged on the bionic trunk paint spraying mechanism, and a second visual sensor, a corrosion sensor and an optical roughness sensor which are electrically connected with the rust removal control assembly are arranged on the bionic trunk rust removal mechanism. The working efficiency of paint spraying and rust removing can be effectively improved in different working environments.

The invention discloses a method for modifying a cellulose acetate filtering stick for a cigarette by adopting high-power electron beams. The method is characterized by comprising the following steps: (1) carrying out transmission irradiation on the cellulose acetate filtering stick in a nitrogen gas or air atmosphere by using the high-power electron beams generated by a high-power electron accelerator or a cobalt source, and leaving the filtering stick to stand after irradiation is finished, wherein the irradiation dose is controlled to be within a range of 50-100 KGy; (2) placing the filtering stick subjected to irradiation modification obtained in the step (1) for 10h under vacuum negative pressure condition with temperature of 40-60 DEG C for removing macromolecules such as free acetic acid or neutralizing acidic components, and drying the filtering stick; and (3) balancing water content in the neutralized or separated filtering stick obtained in the step (2), and directly applying the filtering stick to the split rolling technology for preparing the cigarette.

The present invention screens and isolates a bacterial strain with the best effect of degrading nitrite from mud samples collected from ports such as Haicang and Tong'an in Xiamen City, Fujian Province, and screens and obtains the effect of degrading nitrite from mud flat samples in Ningde City, Fujian Province. Another best strain; the two strains are Rhodopseudomonas palustris PSB20 and Bacillus subtilis ND04 respectively; the microbial preparation prepared by using the two strains can degrade ammonia nitrogen and nitrous acid, and has a good decomposition effect on the residual organic matter of the bait, and can also inhibit the growth of Vibrio pathogenicum in the aquaculture water to a certain extent, that is, it has a good production and application prospect; in addition , the biological preparation also has the characteristics of a simple preparation method.

The invention relates to a Gaussian process based injection molding forming process robust design and tolerance design method. The Gaussian process based injection molding forming process robust design and tolerance design method comprises obtaining the correlation of process parameters and quality objectives by a Gaussian process based self-adaptive canonical correlation analysis method and classify the parameters into main parameters and secondary parameters according to the sizes of degrees of the correlation; establishing a robust optimization model of the main parameters, adding the fluctuation quantity into an objective function and constraint conditions and obtaining a robust process parameter combination through simultaneous optimization of the two targets of the mean and variance to reduce the complexity of calculation of a multi-parameter model reasonably; performing analysis on the relation of the process parameter fluctuation tolerance range and the cost, establishing a mathematical expectation model of a quality overall loss and setting a reasonable tolerance range for every process parameter. According to the Gaussian process based injection molding forming process robust design and tolerance design method, the influence of human experience and trial and error experiment is greatly reduced in the design process, the design cycle is short, the qualified rate of products is high, the system cost is low, an intelligent control project is implemented conveniently, and the Gaussian process based injection molding forming process robust design and tolerance design method can be widely applied to the forming process of various materials.

The invention discloses a method for modifying a cellulose acetate filtering stick for a cigarette by adopting high-power electron beams. The method is characterized by comprising the following steps: (1) carrying out transmission irradiation on the cellulose acetate filtering stick in a nitrogen gas or air atmosphere by using the high-power electron beams generated by a high-power electron accelerator or a cobalt source, and leaving the filtering stick to stand after irradiation is finished, wherein the irradiation dose is controlled to be within a range of 50-100 KGy; (2) placing the filtering stick subjected to irradiation modification obtained in the step (1) for 10h under vacuum negative pressure condition with temperature of 40-60 DEG C for removing macromolecules such as free acetic acid or neutralizing acidic components, and drying the filtering stick; and (3) balancing water content in the neutralized or separated filtering stick obtained in the step (2), and directly applying the filtering stick to the split rolling technology for preparing the cigarette.

The invention provides a preparation method of p-hydroxyacetophenone. Chlorobenzene and acetyl chloride are used as reaction substrates; a Fries rearrangement reaction; acetyl is connected to the para-position of chlorobenzene; chlorine substituents on a benzene ring are hydrolyzed to hydroxyl groups by hydrolysis reaction, and thus the target product is obtained. The preparation method provided by the invention overcomes the problem of more byproducts o-hydroxyacetophenone in a conventional technology, and has the advantages of simple process, fewer types of used raw materials, no excessive discharge of three wastes in the post-treatment process, and good production and application prospects.

The invention discloses a mixed fermentation method of bacillus and bacterial enzymes for efficiently degrading soybean meal antigenic protein. A group of bacillus bacteria that can efficiently degrade soybean meal antigenic protein, including two bacillus strains belonging to Bacillus subtilis subsp.subtilis, are preserved in the General Microorganism Center of China Committee for the Collection of Microbial Cultures, and the preservation numbers are CGMCC NO: 12823, CGMCC NO: 12824. The method of bacteria and enzyme mixed fermentation to degrade the anti-nutritional factors of feed is to make corn, soybean meal and bran into compound feed, add Bacillus fermented seed liquid at 3-5% of the total mass, and add non-starch polysaccharide compound enzyme at 0.5-1% After mixing and stirring evenly, ferment at 30-40°C for 24 hours to obtain a fermented mixed feed, add 5% lactic acid bacteria seed liquid to the fermented mixed feed, mix evenly and package it in an anaerobic fermentation device, and ferment for 48 hours. Get finished feed. The anti-nutritional factor degradation effect of the present invention is obvious, has fermented sour fragrance, good food-attracting effect, and has good production and application prospects.

The present invention relates to a kind of chitinase cm Chi6 gene and its clone expression and application. The present invention clones for the first time the exochitinase with the nucleotide sequence shown in SEQ ID No.1 cm Chi6 gene, and obtained high-activity exochitinase, the preparation method is simple, the production cost is low, the product is easy to store, the suitable enzymatic hydrolysis temperature is 45°C, the optimum pH of enzymolysis is 7.4, at 25-50°C Good thermostability, 33 hours still maintain more than 60% of the enzyme activity; optimum pH 7.4, at pH 7.0‑10.0, 33 hours of enzyme activity still maintain more than 75%; Ba 2+ right cm Chi6 activity has a slight activation effect; cm Chi6 has high degradative activity on colloidal chitin, which can be used to prepare chitobiose with high conversion rate, low energy consumption, and environmental friendliness. It has important economic value in the application of industrial chitin degradation.

The present invention discloses synthetic method of p-N-glyoxalinyl benzaldehyde, which uses appropriate catalyst to increase specific surface area of synthesis reaction, and the invention provides acompletely new subzero fractionating and purifying method, which has the advantages of simply performing, high yield and low cost, and it lays foundation for industrialization production and extensiveapplication prospect of p-N-glyoxalinyl benzaldehyde.

The invention discloses a harvesting method of common coltsfoot flowers. The problems of being low in efficiency and small in harvesting quantity existing in traditional harvesting are solved. The harvesting method of common coltsfoot flowers comprises the following steps of (1) determining a harvesting period: from the first ten-day period of November to the middle ten-day period of November, before soil is frozen, and when bud bracts of the common coltsfoot flowers present claret, performing harvesting; (2) performing mining: digging out underground rhizomes of the common coltsfoot flowers line by line, and during digging out, reserving earth on tubers; (3) performing plant placing: performing monolayer ridge heap shaped plant placing on the mined underground rhizomes for 10-15 days, andperforming dehydration and nutrient ingredient transformation; (4) picking flowers: shaking the rhizomes of the common coltsfoot flowers after planting placing so as to remove earth, then picking offbuds, and performing boxing; and (5) performing drying in shade: placing the boxed common coltsfoot flowers at a ventilated place for spreading for cooling and drying in shade, wherein the spreadingthickness for cooling is 2-3cm, and after moisture dissipates, screening out topsoil so that the common coltsfoot flowers are harvested. Through the adoption of the harvesting method disclosed by theinvention, the harvesting quantity and the harvesting rate of the common coltsfoot flowers can be significantly increased, the labor time can be shortened, and the harvesting method has favorable production and application prospects.

The invention discloses a high-yield soapberry cultivation method, and belongs to the technical field of tree cultivation. The invention provides a set of standard cultivation and management method for soapberry cultivation from the perspective of obtaining high-yield soapberry trees by mastering of the growth characteristics of soapberry by the applicant. The method comprises the following five steps of nursery seedling culture, seedling transplanting, stock grafting, young forest tending and forest management. The soapberry trees cultivated by the method have the characteristics of being high in yield, stable in yield and convenient to harvest; the yield per mu of soapberry fruits can exceed 500 kg at most; and the problems that the soapberry belongs to tall trees, the fruit yield is low in the natural growth state, and picking is relatively difficult are solved. Therefore, the method has good production and application prospects and is suitable for popularization and application.

The invention discloses a heat recycling system and method for VOCs. The system comprises a microwave catalytic combustion device, one end of an air inlet channel is connected with a VOCs air source, the other end is connected with a shell side inlet of a first heat exchanger, a shell side outlet is connected with an air inlet of the microwave catalytic combustion device, an air outlet is connected with a pipe side inlet of the first heat exchanger, a pipe side outlet of the first heat exchanger is connected with a pipe side inlet of a second heat exchanger, a pipe side outlet of the second heat exchanger is connected with a tail gas absorption device, a fresh air device is connected with a shell side inlet of the second heat exchanger, and a shell side outlet is connected with a heat supply pipe network. By means of the microwave catalytic combustion device, VOCs waste gas can be subjected to efficient catalytic oxidation and mineralization, and the degradation and mineralization rate of the VOCs is effectively increased; the first heat exchanger is arranged, preheating of inlet air of the microwave catalytic combustion device is achieved, the temperature rising speed of a catalyst bed layer is increased, the temperature rising time of the catalyst bed layer is shortened, the VOCs waste gas degradation effect is improved, and heat recovery of the VOCs subjected to catalytic oxidation is achieved.

The invention provides a subtilisin-like gene recombination insect killing engineering bacteria and the microbial inoculums preparing method thereof. The insect killing engineering bacteria is a double-effect engineering bacteria which is fused and constructed by the crylAc gene of bacillus thuringiensis and a foreign toxin gene, and expresses fused insecticidal protein crystals in the bacillus thuringiensis. The bacillus thuringiensis is the 4.0718 strain of bacillus thuringiensis. The foreign toxin gene is the CDEP2 gene of subtilisin-like of spherical spore white muscardine fungi. The titers of subtilisin-like gene recombination insect killing engineering bacteria microbial inoculums prepared by fermenting production are all above 6000 international units / microgram, the insect killing rates on the lepidoptera, dipteral and coleopteran insects all reach 90 percent. Under same consistence, the insect killing effect of the invention is 3-5 times higher than that of the monovalent microbial inoculums. The invention has no toxic residue, is safe to human being and animal, and is conducive to protecting entironment.

The invention discloses a metarhizium anisopliae transgenic strain as well as a preparation method and an application thereof. The strain is a recombinant engineering bacterium constructed by fusion of metarhizium anisopliae genes and exogenous Prla (subtilisin-like protease A) genes. The metarhizium anisopliae transgenic strain takes metarhizium anisopliae as the basis, takes Pgpd as a strong promoter, takes TTrpC as a terminator and takes Basta as a selection marker, a Prla overexpression box is constructed with a PCR fusion method, then a fungal protoplast is prepared, genetic engineering transformation is conducted with a CaCl2-PEG method, and the overexpression box is inserted in a metarhizium anisopliae gene group randomly for overexpression. The metarhizium anisopliae transgenic strain is good in German cockroach killing effect, higher in toxicity and capable of effectively controlling German cockroach populations in a short time.

The invention discloses a special seed dressing agent with effects of increasing the survival rate and invigorating seedlings for sweet corn. The seed dressing agent comprises gamma-aminobutyric acid, matrine, thiamethoxam, sedaxane, carboxin, amino-oligosaccharin, salicylic acid, glycine betaine, choline chloride, fulvic acid, starch grafted potassium acrylate, calcium chloride, a wetting dispersant, an emulsifier, gibberellin and microelement water soluble fertilizer mother liquor. Under normal conditions or adverse conditions of drought, low temperature and the like, the seed dressing agent has an obvious effect of improving the seed vigor and seedling quality of the sweet corn, can be used for effectively increasing the yield of the fresh spike of the sweet corn, also has effects of exterminating underground insects, protecting and invigorating seedlings and preventing and treating main plant diseases and insect pests of the corn, has control efficiency up to 70%-90% for plant diseases and insect pests in a seedling stage, has control efficiency up to 87%-96% for corn head smut, and not only can help farmers to save labor cost and seed cost greatly, but also can increase the yield by 15%-20%, thus having excellent social and economic benefits and application prospects.

The invention relates to triton mobilis and application thereof, and belongs to the field of probiotic screening. The invention provides a strain of Tritonibacter mobilis R6-3G2, wherein the preservation number of the Tritonibacter mobilis R6-3G2 is GDMCC (China General Microbiological Culture Collection Center) No: 62115; the DNA gene sequence is as shown in SEQ ID NO. 1. The R6-3G2 can generate an antibacterial compound for inhibiting glass seedling pathogenic bacteria vibrio parahaemolyticus VP-HL. Bacteriostatic experiments prove that the fermentation liquor of R6-3G2 has an inhibition effect on vibrio parahaemolyticus VP-HL; it is proved through a resazurin color development method that intracellular and extracellular metabolic crude extracts of R6-3G2 can inhibit vibrio parahaemolyticus VP-HL; in R6-3G2 prawn culture experiments, the infection of the vibrio parahaemolyticus VP-HL to prawns can be reduced, the survival rate of young prawns is increased, the young prawns have no toxic or side effects, and the vibrio parahaemolyticus VP-HL can be used as probiotics to be added into a water body during culture; the feed can be used as an aquaculture water additive.

The invention discloses a chitin endonuclease CmChi4 as well as the cloning expression and the application of the chitin endonuclease CmChi4. A chitinase gene CmChi4 is constructed on a plasmid pCOLDI through a genetic engineering means, the plasmid pCOLDI is transferred into E.coli BL21 (DE3) for expression, and the molecular weight of the recombinase is 72.1 kDa. Researches on enzymatic properties of CmChi4 show that the activity is optimal when the temperature is 50 DEG C and the pH value is 6; fe <2+>, K<+>, Al<3+> and Zn <2+> have relatively strong activation on the enzyme CmChi4. In addition, the products of the chitin endonuclease CmChi4 for hydrolyzing the colloid chitin are (GlcNAc) 2, (GlcNAc) 3 and (GlcNAc) 4, and the chitin endonuclease CmChi4 is good in stability and suitable for industrialization.