32results about How to "Improve insecticidal virulence" patented technology

The invention discloses a leaf vegetable type vegetable organic fertilizer and a preparation method thereof, and relates to the technical field of organic fertilizer. The leaf vegetable type vegetable organic fertilizer is prepared from 60-70 parts of humic acid fertilizer, 55-65 parts of plant ash, 45-55 parts of peanut meal, 45-50 parts of carrier components, 35-45 parts of cornstalk, 35-40 parts of pond sludge, 20-30 parts of urea, 20-30 parts of disinsectization components, 15-25 parts of beet, 15-20 parts of saccharose, 15-18 parts of edible mushroom dregs, 10-15 parts of organic material leavening agent, 10-12 parts of xylitol residues, 5-10 parts of pig manure, 2-8 parts of ammonium sulfate, 2-6 parts of magnesium sulfate and 90-100 parts of water. The leaf vegetable type vegetable organic fertilizer can not only promote crop growth of leaf vegetables, improve the crop quality and but also effectively prevent plant diseases and insect pests.

The invention discloses a compound pesticide, which is mainly prepared from the following raw materials in percentage by weight: 5-20% of azadirachtin and metarrhizium, 8-12% of emulsifier, 3-7% of stabilizer, 2-8% of synergist, 0.1-1.0% of antioxidant, 10-18% of cosolvent and 50-60% of organic solvent. The compound pesticide is environment-friendly, has high compatibility and obvious synergisticeffect, and can obviously improve the insecticidal toxicity of a single dosage and reduce the use of high-persistent chemical pesticide.

The invention discloses a metarhizium anisopliae transgenic strain as well as a preparation method and an application thereof. The strain is a recombinant engineering bacterium constructed by fusion of metarhizium anisopliae genes and exogenous Prla (subtilisin-like protease A) genes. The metarhizium anisopliae transgenic strain takes metarhizium anisopliae as the basis, takes Pgpd as a strong promoter, takes TTrpC as a terminator and takes Basta as a selection marker, a Prla overexpression box is constructed with a PCR fusion method, then a fungal protoplast is prepared, genetic engineering transformation is conducted with a CaCl2-PEG method, and the overexpression box is inserted in a metarhizium anisopliae gene group randomly for overexpression. The metarhizium anisopliae transgenic strain is good in German cockroach killing effect, higher in toxicity and capable of effectively controlling German cockroach populations in a short time.

The invention discloses a biogenic insecticide mixture controlling pests. The biogenic insecticide mixture is prepared from, by weight, 40-60 parts of a bacillus thuringiensis fermentation solution, 10-20 parts of a lactic acid bacteria fermented solution, 10-20 parts of fresh anisodus acutangulus leaves, 10-20 parts of fresh leaves of camphor trees and 0.05-0.1 part of Tween 20. According to the biogenic insecticide mixture, the selectivity of selected bacillus thuringiensis is high, damage to people and livestock does not exist, the biogenic insecticide mixture belongs to biological insecticide sources very high in safety, lactic acid bacteria belong to probiotics, the lactic acid bacteria are used for preparing an insecticide for the first time, the toxicity titer is high, time needed for disinsection is short, a preservative does not need to be additionally added, chemical substances are reduced, and the problem that residual time of chemical pesticide is long is solved. In addition, the invention discloses a preparation method and application of the biogenic insecticide mixture.

The invention relates to a method for acquiring high-activity Cry1Ai protein mutants and the mutants, and belongs to the biotechnology field. The method for acquiring the high-activity Cry1Ai protein mutants comprises the steps that continuous multi-point mutation is performed on an amino acid sequence in a loop 2 or/and a loop 3 of a Cry protein Domain II and comprises more than 10 continuous amino acid sequence changing. The insecticidal activity of the continuous multi-point mutant ModCry1Aid-loop 2 on cotton bollworm is improved by about 13 times, the activity of the continuous multi-point mutant ModCry1Aid-loop 2 on ostrinia nubilalis is improved by near 9 times compared with a Cry1Ai protein before improvement, and the activity of the continuous multi-point mutant ModCry1Aid-loop 2 and the continuous multi-point mutant ModCry1Aid-loop 3 on rice stem borer is improved by about 5-6 times compared with the Cry1Ai protein before improvement.

A recombinant autographa california multiple nuclear polybedrosis virus, its preparing process, and its application as insecticide with broad spectrum and high speed are disclosed.

The invention discloses a pleocidin and athomin compounded composition and application thereof.The effective ingredients of the compounded composition comprise pleocidin and athomin.A method in the technical field is adopted, the compounded composition can be prepared into emulsion in water or microemulsion or a suspending agent or wettable powder or water dispersible granules or the like.The pleocidin and athomin compounded composition has an obvious synergistic effect as a protective agent for grain storage, the insect killing toxicity is obviously higher than that of any single agent, the double effects of insect killing and mould preventing are achieved, and the insecticidal spectrum and the mould proof spectrum are wide.

The invention relates to an insecticidal composition with synergistic effect. The basic active components of the insecticidal composition are destruxins and diflubenzuron which are mixed according to a ratio of 1-9 g: 1-9 g. Adjuvants and excipients are added into the active components for preparation of a wettable powder, a suspending agent or an emulsion in water. The insecticidal composition can be applied to prevention and treatment of diamondback moth, prodenia litura, Lithocolletis ringoniella, grubs, wireworms, Trialeurodes vaporariorum, Liriomyza sativae, root maggots and Bradysia odoriphaga, preferably to prevention and treatment of lepidoptera insects like diamondback moth and prodenia litura on cruciferous vegetable, and has the advantages of stable efficacy, fast action, long action period, safety, environmental protection, etc.

The invention discloses an allyl isothiocyanate and allicin compound and an application thereof. The compound is prepared from allyl isothiocyanate (AITc), allicin and an organic solvent, and can be applied in food storage as a fumigation agent for food storage. The allyl isothiocyanate and allicin compound disclosed by the invention has an obvious synergistic effect; the insecticidal toxicity is obviously higher than that of any single dosage; the insecticidal bacteriostatic spectrum is obviously expanded; the control effect on pests, such as tribolium castaneum, oryzae philus surinamensis, sitophilus zeamais, grain moth and cryptolestes ferrugineus, in stored grain reaches over 95%; the bacteriostasis rate on a plurality of stored grain mildews such as aspergillus oryzae, aspergillus flavus, blue mould and aspergillus niger reaches over 95%; and the allyl isothiocyanate and allicin compound has relatively high cost advantages, economic benefits and relatively good control effect.

A Bacillus thuringiensis WY-190 (CCTCC No.M202005) for killing beet noctuids is prepared through loading soil sample in test tube containing nutritive liquid, vibration culture, diltuing, coating on discrete culture medium, inversion culture, purifying monoclonly, inoculating to fermenting culture medium, shake-flask fermentation until parasporal crystal is dropped, mixing with artificial feed, and biological test with target insect. Its advantage is high insecticiding toxin.

The invention relates to an insecticidal composition with a synergistic action. The insecticidal composition is prepared by taking destruxin and pyriproxyfen as basic active components and mixing the destruxin and the pyriproxyfen according to the following ratio of 1g to (1-99)g. After the two effective components are mixed, the insecticidal composition can be applied to preventing and controlling health pests including aedes albopictus, flies and termites and preferably preventing and controlling diptera health pests including mosquitos, flies and the like.

The invention belongs to the technical field of genetic engineering, and specifically relates to a synthetic bacillus thuringiensis insecticidal gene with virulence against lepidoptera. The synthetic gene comprises a nucleotide sequence of a Vip83Cry9Ca<*> gene and an amino acid sequence coded by the gene. Escherichia coli DH5alpha/Bt./6p/pGEVC9C comprising the gene plasmid is collected at China Center for Type CultureCollection (CCTCC), and has a collection number of CCTCC NO: M2011363. As a result of biological experiments, the insecticidal fusion gene has virulence against lepidoptera, and can specifically kill agricultural pests such as diamondback moth and the like. The invention also discloses an application method of the fusion protein in the respect of transgenic insecticidal microbes.

The invention discloses an insecticide for corn seedlings. The insecticide takes celastrus angulatus extract, garden balsam stem extract and pyrethroid as main components and is specifically preparedfrom the following components in parts by weight: 3-5 parts of celastrus angulatus extract, 15-22 parts of garden balsam stem extract, 3.5-4.6 parts of pyrethroid, 0.2-1.3 parts of xanthan gum, 0.6-2.6 parts of Tween-20 and 52-64 parts of water. The insecticide disclosed by the invention has the beneficial effects that all components can exert mutually synergistic effects; the insecticide is broadin spectrum, high in efficiency, not liable to generate resistance to drugs, low in toxicity, easy to degrade and free of residues; the insecticide for corn seedlings has better mobility in soil layers and has higher content of haedoxan A.