49results about How to "Simplify experimental procedures" patented technology

{0><}0{>The invention relates to a method for measuring contents of gold and silver in a gold mud sample by virtue of fire assay and belongs to a method for measuring a gold mud material by virtue of a fire assay method. <0}{0><}0{>The method comprises the following steps of: burdening and fusing a test sample so as to obtain a proper amount of lead buttons and fragile molten slag; recycling residual gold and silver in the molten slag; separating gold and silver from the lead buttons through cupelling, thereby obtaining gold and silver combination particles; processing and weighing the particles; grinding the gold and silver combination particles into slices; separating gold by use of nitric acid; and weighing, and calculating contents of gold and silver. <0}{0><}0{>The method is capable of improving the experiment accuracy and simplifying experimental procedures, is convenient to operate, and is capable of realizing batch operation for samples and effectively improving the working efficiency.

The invention discloses a fabrication method of cover glass for conidiospores of Ophio cordyceps sinensis and a dynamic counting method of produced conidiospores. The method comprises inoculating Ophio cordyceps sinensis at each hole on a solid culture medium containing at least one hole to obtain at least one inoculated hole; arranging a piece of cover glass above each inoculated hole to obtain a cover glass plate; and culturing the cover glass plate until a colony grows, taking 3 pieces of cover glass every a period of time, and counting to obtain the number of conidiospores produced by Ophio cordyceps sinensis at different moments. According to the experiment, the invention simplifies experimental operation, can in-situ observe and count at fixed points through an inverted microscope, and objectively reflects the conidiospore production cycle of Ophio cordyceps sinensis strains.

The invention discloses a fluorescence and/or radionuclide containing nanometer kit for detecting specific activated protease of serum and a preparation method and application of the kit. The kit is prepared from the following components: magnetic nanoparticles, protease-specific cut peptide chain, and fluorescent molecular and/or radionuclide; the preparation method comprises the steps of preparing the nanoparticles, synthesizing the peptide chain, connecting the fluorescent molecular and/or radionuclide and constructing a nanometer platform. The fluorescence and/or radionuclide containing nanometer kit for detecting specific activated protease of serum can be applied to detection of tumor-specific protease of solid tumors such as breast cancer, non-small cell lung cancer, thyroid cancer, liver cancer, ovarian cancer, endometrial cancer, pancreatic cancer, prostate cancer, glioma and melanoma; all components of the nanometer platform are non-toxic and high in biological applicability, protease-specific cut peptide chain has high-sensitive responsiveness to tumor-specific protease and the detection method is rapid, accurate and specific.

The invention belongs to the field of biological analysis, and relates to a fluorescent probe based on oxidative damage bases, and a test kit and a method for directly detecting DNA methylation. The fluorescent probe based on oxidative damage bases is developed for the first time, and DNA methylation of a single site is accurately detected through cyclic cutting signal amplification. hOGG1 enzymecan specifically cut an 8-oxoG base group in an 8-oxoG/5-mC base pair, but has no activity on the 8-oxoG base group in an 8-oxoG/U base pair. The presence of the target methylated DNA can induce cyclic cleavage of the fluorescent probe with the aid of the hOGG1 enzyme resulting in an enhanced fluorescent signal. The method is high in sensitivity and high in specificity, a single methylation site can be detected, and the lower detection limit reaches 3.458 * 10 <-15> M. And the method can distinguish 0.01% of methylation level in a complex DNA mixed system, and can also detect the DNA methylation level in the genome.

The invention discloses a flow speed measurement instrument in micro flow, which is applied to micro flow experiments for petroleum recovery rate. The flow speed measurement instrument is characterized in that at least three sections of micro speed measurement tubes are horizontally fixed on a fixed board, and one end of a first section of micro speed measurement tube is connected to an inlet tube line. The other end of the first section of micro speed measurement tube is connected with a tee, and a vertical end of the tee is connected with a section increasing plug tube line. The other end of the tee is horizontally connected with a second section of micro speed measurement tube. A scale is fixed along and beside the second section of micro speed measurement tube. The other end of the second section of micro speed measurement tube is connected with a bent tube and a micro speed measurement tube. The end part of the last section of micro speed measurement tube is connected with an outlet tube line. The flow speed measurement instrument has the effects that the instrument can quantitatively measure the seepage speed of the fluid in a micro model, can quantify the instantaneous seepage speed in the micro model, realizes the measurement for the seepage speed under a high pressure condition, and improves the measuring precision of micro model displacement experiments.

The invention provides a method for preserving the callus of rubber plant anther with vitrification and super low temperature, comprising the following steps: the anther callus is inoculated to the pre-culture medium, dark culture is carried out for 2 to 3 days with the culture temperature of 25 to 28 DEG C; the anther callus is processed by 60 percent of PVS2 for 10 to 30 minutes at room temperature; after dehydration process, the anther callus is processed by the PVS2 for 20 to 40 minutes at the temperature of 0 DEG C, the stock solution of the PVS2 is removed, then new PVS2 is added, and the anther callus is stocked in liquid nitrogen; and after the anther callus is resurgent, successive transfer culture is carried out, the regeneration plant can be obtained after further embryoid is induced and the mature embryoid is induced to carry out differentiation. The preservation method by liquid nitrogen with vitrification and super low temperature related by the invention has the advantages of simple equipment, simple experiment procedure and good effect and repetitiveness, etc., can preserve the callus of rubber plant anther for long time, obtains the regeneration plant by culturing after unfrozen and creates conditions for the genetic transformation of the rubber plant.

The invention provides a method for determining the electron transfer rate of a liquid/liquid interface, and the method comprises the following steps: through combining a computer simulation method with a thin-layer cyclic voltammetry, based on the liquid/liquid interface electron transfer theory of the thin-layer cyclic voltammetry, carrying out simulation and forecasting on a thin-layer cyclic voltammetry model through numerical simulation firstly so as to obtain the optimal experiment condition parameter; then, applying the optimal experiment condition to a specific experimental operation so as to obtain a series of ideal cathode platform current values; and finally, carrying out linear fitting on the cathode platform current values so as to obtain the precise constant value of the electron transfer rate of the liquid/liquid interface. In the method disclosed by the invention, the optimal experiment condition is determined by taking computer simulation as the guide and then applied to the specific experimental operation, therefore, the method disclosed by the invention is a new operable method which integrates the advantages of the computer simulation method and the thin-layer cyclic voltammetry, therefore, by using the method, the determination on the electron transfer rate of the liquid/liquid interface is more convenient, quick and accurate.

The invention relates to a three-dimensional physical similarity simulation experimental frame with an adjustable angle. The three-dimensional physical similarity simulation experimental frame comprises an external structural frame and an internal frame, wherein two ends of the external structural frame are provided with an angle determining plate, and each angle determining plate is provided with an angle adjusting track; the bottoms of the front side and the rear side of the internal frame are respectively provided with a guide pulley, the rear end guide pulley is movably hinged with the external structural frame, and the front end guide pulley is matched with the angle adjusting track; and the bottom of the external structural frame is provided with a hydraulic cylinder and a support thereof, and the hydraulic cylinder acts on the internal frame. The three-dimensional physical similarity simulation experimental frame has the advantages that: 1, the operation of the frame is simple, more manpower is saved, and the success rate of experiments is improved; 2, the edge of the frame is provided with an angle value, the angle for the experiment can be directly adjusted, and the experimental process is simplified; 3, the periphery of the frame is protected by using a high-strength transparent materialboard, so that the phenomenon is easy to observe, and results are more realistic; and 4, an open-off cut is reserved, so that the experimental procedure can be simplified, and the experiment is more realistic and simpler.

The invention discloses a method for detecting a PCR amplification product. The method is characterized by comprising the following steps: (1) adding a fluorescent dye and a nonionic detergent into aPCR reaction system, and then carrying out PCR amplification, wherein the concentration of the fluorescent dye in the PCR reaction system is 0.1-1X, and the concentration of the nonionic detergent inthe PCR reaction system is 0.1-0.5%; and (2) observing the system obtained in the step (1) by naked eyes in a dark field under irradiation of an ultraviolet lamp, or observing the system subjected tophotographing by a fluorescent imaging system, and judging whether the PCR amplification product exists or not through the color of the system. For PCR identification which is judged by whether reacting occurs or not, the method can be used for direct judging by observation in a PCR tube by using the fluorescent imaging system or naked eyes without gel electrophoresis, so that experimental procedures are reduced, experimental operation is reduced, time is saved, and efficiency is improved.