91results about How to "Mark stable" patented technology

The invention discloses a chemiluminescent detection kit for interleukin-6 and a preparation method thereof. The kit includes: sample diluent, magnetic particles coated with interleukin-6 monoclonal antibody, interleukin-6 monoclonal antibody labeled with acridinium ester, interleukin-6 series standard solution, chemiluminescence excitation solution A, chemiluminescence excitation solution B. Cleaning solution. The kit of the invention combines the chemiluminescent technology with the immunomagnetic particle, and provides a nearly homogeneous reaction system. Compared with the prior art, the kit of the invention has the advantages of high sensitivity, strong specificity, short reaction time and the like.

The invention discloses a chemiluminescent detection kit for procalcitonin and a preparation method thereof. The kit includes: sample diluent, magnetic particles coated with procalcitonin monoclonal antibody, procalcitonin monoclonal antibody labeled with acridinium ester, procalcitonin series standard solution, chemiluminescence excitation solution A, Chemiluminescence excitation solution B, cleaning solution. The kit of the invention combines the chemiluminescent technology with the immunomagnetic particle, and provides a nearly homogeneous reaction system. Compared with the prior art, the kit of the invention has the advantages of high sensitivity, strong specificity, short reaction time and the like.

The invention relates to a molecular marker for mungbean weevil resistance gene linkage, which is used for screening mungbean weevil resistant genes. V2709, VC1973 (zhonglu No. 1) and the individual DNA of F2 separation population thereof are extracted by the CTAB method. 63 RAPD molecular markers, 100 pairs of SSR molecular markers and 28 pairs of STS molecular markers are adopted to carry out the PCR product polymorphism screening on the resistant bulk and the susceptible bulk of the two parents and the F2 progeny, and then each of F2 generation individuals is screened out the weevil resistance gene linkage marker. A RAPD primer, OPC-06 and a STS primer are found to link with a weevil resistance genes The molecular marker can target the weevil resistance gene, and the selection efficiency of weevil resistance breeding is greatly improved.

The invention belongs to the field of molecular imaging reagents ad relates to an active near infrared fluorophore for rapidly marking bioactive molecules. A general formula of the active near infrared fluorophore is IRP-B-NHS, wherein IRP is an anthocyanin near infrared fluorophore; B is an aromatic group introduced to the secondary position of the anthocyanin fluorophore by virtue of a carbon-carbon bond; NHS is N-hydroxysuccinimide eater. A preparation method of the active near infrared fluorophore comprises the following steps: with the anthocyanin fluorophore as a parent, introducing benzene carboxylic acid into the fluorophore by virtue of the carbon-carbon bond through Suzuki-Miyaura reaction, modifying phenyl carboxylic acid to produce the N-hydroxysuccinimide eater, reacting with primary amine in a biomolecule under the physiological condition, and marking the biomolecule with the near infrared fluorophore to realize noninvasive tracing. The active near infrared fluorophore is capable of rapidly, safely, effectively and stably marking the bioactive molecules including polypeptide, proteins, antibodies or polymer molecules and has important significance of noninvasively monitoring and quantifying distribution of target active molecules in vivo.

The invention provides a method for identifying soybean male sterile cytoplasm through SNP marks of chloroplast DNA. The method can also be applied to distinction of a soybean cytoplasmic male sterile line and a maintainer line containing normal cytoplasm. The method includes the following steps of extracting the seed genome DNA of the soybean cytoplasmic male sterile line and the seed genome DNA of the maintainer line to serve as amplification templates, selecting four pairs of the SNPs marks of the chloroplast DNA, and designing a pair of primers on the side wing sequences of each pair of SNPs respectively to conduct PCR amplification. The PCR products obtained through amplification conduct identification on the difference between the length of the enzyme-digested products of the sterile line and the length of the enzyme-digested products of the maintainer line through digestion of restriction enzymes. According to the method, the soybean male sterile cytoplasm and normal fertile cytoplasm can be rapidly and accurately identified. The method can also be used for detecting the maintainer line which contains fertile cytoplasm and is mixed in the sterile line containing sterile cytoplasm in the breeding process of the sterile line, and provides guarantees for the purity requirement in the production process of soybean sterile line seeds.

The invention relates to a non-heading cabbage male sterile molecule marking assistant selecting method. Using marking primer of OPAG20 or SCAG20 to amplify non-heading cabbage male sterile series or breeding material chondriosome DNA, if the 700bp section is amplified, the male sterile gene is existed. The invention could be used to taking selecting to PAPD and SCAR molecule marking for non-heading cabbage male sterile series 98HA and the maintainer line 98HB chondriosome DNA to improve selecting efficiency and speed up breeding process.

The invention relates to a 25-hydroxyvitamin D TRFIA (Time-Resolved Fluoroimmunoassay) kit and a detection method, which utilize the sensitivity of TRFIA technology, utilize rare earth vanadate nano fluorescent labeling materials and combine a dry-type immunofluorescence analyzer to realize high sensitivity, accurate quantification, simpleness and convenience. The kit of the invention can accurately and quantitatively detect the content of the 25-hydroxyvitamin D in serum, plasma and whole blood samples of persons, the whole blood samples can be filtered through the treatment of blood filtering membrane sample pads, the fluorescence interference of the samples can be avoided by utilization of a time resolution detection technology, the fluorescent microspheres are rare earth vanadate nanomaterials, thus the advantages of low background, strong fluorescence signals, high signal-to-noise ratio and the like are achieved, rare earth vanadate nano particles are connected with antibody through covalent bonds by labelling, a labeled product is stable, thus the kit has the characteristics of wide detection range (3-100ng/mL), high sensitivity (detection limit is 3ng/mL), high accuracy, quick and convenient detection and the like.

The invention belongs to the technical field of biology, and relates to a method for quickly identifying the genetic purity of a glutinous corn hybrid. The method comprises the following steps of: extracting genomic deoxyribonucleic acid (DNA) from glutinous corn, performing polymerase chain reaction (PCR) amplification by using the screened sequence-related amplified polymorphism (SRAP) effective primer combination NAUSRem7/NAUSRpm1 and random amplified polymorphic DNA (RAPD) effective primers NAUSR709 and NAUSR712, respectively performing non-denaturing polyacrylamide gel electrophoresis and agarose gel electrophoresis on a PCR product obtained through amplification, and shooting a DNA electrophoresis pattern; and comparing and analyzing the size and position difference of polymorphism amplified fragments formed due to the difference of DNA fragment sequences in the electrophoresis pattern, and identifying the genetic purity of the glutinous corn F1 hybrid, namely a Suyunuo 2 seed. The detection method has the advantages of marking stability, high accuracy, no influence of the growth stage and environment of a sample to be detected, low cost, capability of being performed in thewhole growth season, and the like.

The invention relates to a molecular marker for co-separating from aroma characters, belonging to the field of molecular genetics. 1-2 pieces of spires at the upper part of individual rice are taken at the tillering stage and are reserved in a refrigerator with the temperature of below 80 DEG C for later use. Rice leaves with the length of 2-4cm are taken to be placed in a centrifugal tube of 1.5ml, liquid nitrogen is added, grinding is carried out, 900 mu l of TPS solution is added, and water bath is performed for 20min at the temperature of 75 DEG C. A cover is opened to smell the odour by a nose to determine whether aroma exists. A specific PCR primer L01 or L02 is utilized to obtain the molecular marker by PCR amplification. The invention can be specially used for seed selection and genetic research of the rice aroma variety and can be used for cloning aroma genes.

The invention relates to an auxiliary selecting method of a ground-cover chrysanthemum plant type creeping molecule mark, which belongs to the technical field of biology and which can be used for auxiliary selecting and breeding for the ground-cover chrysanthemum plant type creeping molecule mark. RAPD primer A-10 or an SCAR primer is used to augmentground-cover chrysanthemum or the breeding material DNA; if the fragment of 555bp can be augmented, the existence of a ground-cover chrysanthemum creeping is proofed. The method takes 152 plants of F1 segregation population obtained by taking 'early red Italy' (P1) as a female parent and takes 03(6)-12 as a male parent (P2) as the testing materials to build a plant type creeping/vertical gene pool to carry out screening on the RAPD and SCAR molecule marks, which can effectively develop the auxiliary breeding for the ground-cover chrysanthemum plant type creeping molecule mark and greatly improve the selecting efficiency, thereby quickening the breeding process.

The invention discloses development and application of a KASP marker of an oryza sativa high-temperature-resistant gene TT1, and belongs to the technical field of gene biology. The molecular marker is a KASP marker KASP-TT1 which is closely linked with an oryza sativa high-temperature-resistant gene TT1, the KASP-TT1 is an SNP site in an oryza sativa genome, the nucleotide type of the KASP-TT1 is A or G, and the KASP-TT1 is the 101 nucleotide of SEQ ID No.4 in a sequence table. The KASP technology is applied to perform genotype identification on the oryza sativa high-temperature-resistant gene TT1, the method has the advantages of simplicity and convenience in operation, low cost, short detection period, stability in marking, environment friendliness and the like, the oryza sativa high-temperature-resistant gene TT1 can be accurately detected, and the method has important significance in promoting high-temperature-resistant oryza sativa breeding.

The invention belongs to the field of fluorescent imaging reagents and relates to a dark red fluorescent active ester IR640B-NHS which can be rapidly marked, and a precursor IR640B of the ester. According to the ester, 2,3,3-trimethylindole is adopted as a matrix which reacts with raw materials such as butane sultone, then a cyanine type fluorescent group with a sulfonic group is synthesized, further through a Suzuki-Miyaura reaction, phenyl carboxylic acid is introduced into a fluorescent group through a carbon-carbon bond, and the phenyl carboxylic acid is modified to generate an N-hydroxylcarboxyfluorescein diacetate succinimidyl ester. The active ester can react with a primary amino group in biological macromolecules under physiological conditions, and then dark red fluorescent groupscan be marked on target molecules through amide bonds. By adopting the ester, biological macromolecules such as polypeptide, proteins, antibodies or polymer molecules can be rapidly, safely, effectively and stably marked, in-vitro evaluation on acceptor targeting properties and intracellular distribution of target biological macromolecules can be implemented by using a fluorescence microscope, aflow cytometer and the like, and nondestructive monitoring and quantitative tracing on target molecules can be achieved through living optical imaging.

The invention belongs to the technical field of biology and discloses a chrysanthemum amenone form molecular marker assistant selection method. A molecular marker is an SRAP molecular marker or/and SCAR marker; in the method, 80 F1 segregating populations, acquired by hybridizing amenone form spray cut chrysanthemum variety Nannong Xuefeng as a female plant and non amenone form variety QX096 as amale plant, are taken as a test material; specific sites, linked to amenone form genes, are screened and controlled by using the SRAP molecular marker, wherein SRAP molecular marker primer combinationM11E1 is amplified in an amenone form chrysanthemum material to obtain a specific fragment of 272bp; by designing a specific SCAR molecular marker primer, amplification is performed in an amenone material to obtain a single band of 168bp; it is further verified in two populations of Nannong Xuefeng*QX096 and Nannong Xuefeng*Mengbai that the accuracy reaches up to 92.5 percent and 84.3 percent respectively; and the situation that the marker is successfully transformed into a SCAR molecular marker is explained. The method provided by the invention improves the selection efficiency of an amenoneform marker and accelerates the selecting process of a new variety of the amenone form chrysanthemum.

The invention relates to the molecule marking method for non-heading cabbage male sterile gene that takes selecting of PAPD and SCAR molecule mark for non-heading cabbage male sterile series 98HA and maintainer line 98HB to gain two marks of non-heading cabbage male sterile genes--NAU/RAPD/CMS1800 and NAU/RAPD/CMS2600 and transforming it to two SCAR marks NAU/SCAR/CMS3800 and NAU/SCAR/CMS4600. The invention could improve selecting efficiency and speed up breeding process.