Markable dark red fluorescent active ester

Abstract

The invention belongs to the field of fluorescent imaging reagents and relates to a dark red fluorescent active ester IR640B-NHS which can be rapidly marked, and a precursor IR640B of the ester. According to the ester, 2,3,3-trimethylindole is adopted as a matrix which reacts with raw materials such as butane sultone, then a cyanine type fluorescent group with a sulfonic group is synthesized, further through a Suzuki-Miyaura reaction, phenyl carboxylic acid is introduced into a fluorescent group through a carbon-carbon bond, and the phenyl carboxylic acid is modified to generate an N-hydroxylcarboxyfluorescein diacetate succinimidyl ester. The active ester can react with a primary amino group in biological macromolecules under physiological conditions, and then dark red fluorescent groupscan be marked on target molecules through amide bonds. By adopting the ester, biological macromolecules such as polypeptide, proteins, antibodies or polymer molecules can be rapidly, safely, effectively and stably marked, in-vitro evaluation on acceptor targeting properties and intracellular distribution of target biological macromolecules can be implemented by using a fluorescence microscope, aflow cytometer and the like, and nondestructive monitoring and quantitative tracing on target molecules can be achieved through living optical imaging.

Description

technical field [0001] The invention belongs to the field of fluorescent imaging reagents and relates to a markable deep red fluorescent active ester, in particular to a class of active deep red fluorescent active ester and its precursor which can quickly label target biomolecules. The invention provides the synthesis, characterization, optical property measurement and application in biomolecular labeling of the active deep red fluorescent active ester and its precursor. Background technique [0002] The prior art discloses that the so-called imaging system is to convert some phenomena or processes that cannot be directly observed by the naked eye into visual images through a certain way, so as to facilitate observation and research by the observer. According to reports, the German physicist Roentgen accidentally discovered for the first time that X-rays can penetrate the human body for imaging. Only then did scientists pay more attention to imaging and further research and ...

AI Technical Summary

Problems solved by technology

[0005] So far, there have been no reports of the above-mentioned fluorescent dyes being used as deep red fluorescent probes for bioactive molecule labeling.

Images