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Labeled Alkaline Phosphatase Antigen Antibody Diluent

A technology of antigen antibody and diluent, applied in the direction of enzyme stability, etc., can solve the problem of low sensitivity of labeling reagents

Active Publication Date: 2016-05-11
AILEX TECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Domestic manufacturers who use enzymes as amplification systems mostly use horseradish peroxidase, the main reason is that the enzyme is cheap and the labeling reagent is relatively stable in storage, but the horseradish peroxidase labeling reagent is more sensitive than alkaline phosphatase Low
The reason why alkaline phosphatase is not widely used is the stability of alkaline phosphatase labeling reagents.

Method used

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  • Labeled Alkaline Phosphatase Antigen Antibody Diluent
  • Labeled Alkaline Phosphatase Antigen Antibody Diluent
  • Labeled Alkaline Phosphatase Antigen Antibody Diluent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The diluent of the present invention is configured from the following materials.

[0024] Phosphate buffer:

[0025] NaCl8.0g;

[0026] KCl0.2g;

[0027] Na 2 HPO 4 .12H 2 O2.9g;

[0028] K H 2 PO 4 0.2g;

[0029] Adjust the pH to 7.4, add DDW to make up to 1000mL.

[0030] Polyhydroxy organic compounds:

[0031] Mannitol 30.0g.

[0032] Amino acids and protein compounds:

[0033] BSA (Bovine Serum Albumin) 10.0g;

[0034] Glycine 20.0g.

[0035] Metal ion:

[0036] MgCl21mM;

[0037] ZnCl20.1mM;

[0038] Nonionic Surfactants:

[0039] TweenX-200.5ml.

[0040] preservative:

[0041] NaN3 (sodium azide) 0.90 g.

[0042] Dilute the concentrated solution of AFP antibody labeled with alkaline phosphatase with a diluent at 1:4000 to obtain an enzyme-labeled reagent. The reagent is divided into two parts, one part is placed in a 37°C incubator for accelerated testing, and the other part is Store in a refrigerator at 2-8°C. After accelerating for 7 days a...

Embodiment 2

[0045] Phosphate buffer:

[0046] NaCl8.0g;

[0047] KCl0.2g;

[0048] Na 2 HPO 4 .12H 2 O2.9g;

[0049] K H 2 PO 4 0.2g;

[0050] Adjust the pH to 7.4, add DDW to make up to 1000mL.

[0051] Polyhydroxy organic compounds:

[0052] Sucrose 25.0g.

[0053] Amino acids and protein compounds:

[0054] BSA (Bovine Serum Albumin) 10.0g;

[0055] Valine 20.0g.

[0056] Metal ion:

[0057] MgCl21mM;

[0058] ZnCl20.1mM;

[0059] Nonionic Surfactants:

[0060] TweenX-1000.5ml.

[0061] preservative:

[0062] Proclin-3000.5ml.

[0063] Dilute the concentrated solution of hepatitis B surface antibody labeled with alkaline phosphatase with a diluent at a rate of 1:2000 to obtain an enzyme-labeled reagent. Store in a refrigerator at 2-8°C. After accelerating at 37°C for 7 days, take out the enzyme-labeled reagent, and use the enzyme-labeled reagent at 2-8°C as a control, add the ELISA plate wells coated with hepatitis B surface antibody, add hepatitis B surface antige...

Embodiment 3

[0066] Phosphate buffer:

[0067] NaCl8.0g;

[0068] KCl0.2g;

[0069] Na 2 HPO 4 .12H 2 O2.9g;

[0070] K H 2 PO 4 0.2g;

[0071] Adjust the pH to 7.4, add DDW to make 1000mL.

[0072] Polyhydroxy organic compounds:

[0073] Sucrose 25.0g.

[0074] Galactose 20.0g

[0075] Amino acids and protein compounds:

[0076] BSA (Bovine Serum Albumin) 10.0g;

[0077] Casein 10.0g.

[0078] Metal ion:

[0079] MgCl 21mM.

[0080] Nonionic Surfactants:

[0081] TweenX-1000.5ml.

[0082] preservative:

[0083] Proclin-3000.5ml.

[0084] Dilute the concentrated solution of hepatitis B core antibody labeled with alkaline phosphatase with a diluent at 1:12000 to obtain an enzyme-labeled reagent. Store in a refrigerator at 2-8°C. After accelerating for 7 days at 37°C, take out the enzyme-labeled reagent, and use the enzyme-labeled reagent at 2-8°C as a control, add it to the wells of the ELISA plate coated with hepatitis B core antigen, add hepatitis B core antibody to com...

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Abstract

The invention relates to a medical reagent, and particularly relates to an alkaline phosphatase labeled antigen-antibody diluent. The diluent is characterized by consisting of the following substances: a phosphate buffer, polyhydroxy organic compounds, amino acid and protein compounds, metal ions, a nonionic surface active agent, and a preservative. Compared with the prior art, the diluent provided in the invention can stabilize the activity of an alkaline phosphatase labeled antigen-antibody, and the alkaline phosphatase labeled antigen-antibody is prepared into a lyophilized product by using the freeze-drying method by adding the diluent into the alkaline phosphatase labeled antigen-antibody, so that the activity of the alkaline phosphatase labeled antigen-antibody can be stabilized in a long term.

Description

[technical field] [0001] The invention relates to a pharmaceutical reagent, in particular to a diluent labeled alkaline phosphatase antigen antibody. The diluent can stabilize the activity of antigen-antibody labeled alkaline phosphatase, and can stabilize the activity of antigen-antibody labeled alkaline phosphatase for a long time by freeze-drying method, which greatly improves the use of alkaline phosphatase in the field of immunological in vitro diagnostics. Applications. [Background technique] [0002] Alkaline phosphatase (ALP) is an enzyme widely present in animals, plants and microorganisms. Alkaline phosphatase, which is often used in clinical in vitro diagnosis, is mainly obtained from calf intestinal mucosa and Escherichia coli. Both enzymes can be purchased in the market such as Roche and Sigma. Alkaline phosphatase can catalyze the color development of a variety of substrates, so it has a wide range of applications in the clinical immunological in vitro diagno...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/96
Inventor 李子樵饶啊文
Owner AILEX TECH GRP CO LTD
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