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Molecule labeling method for non-heading Chinese cabbage late bolting gene

A technology of head cabbage and molecular markers, which is applied in biochemical equipment and methods, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of yield and quality decline, and achieve accelerated breeding process, long cycle, and realization of The effect of seedling selection

Active Publication Date: 2010-07-07
南京农业大学资产经营有限公司
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Problems solved by technology

However, due to early bolting, its yield and quality have decreased significantly. Therefore, late bolting has become an important breeding target for non-heading Chinese cabbage.

Method used

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  • Molecule labeling method for non-heading Chinese cabbage late bolting gene
  • Molecule labeling method for non-heading Chinese cabbage late bolting gene
  • Molecule labeling method for non-heading Chinese cabbage late bolting gene

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Embodiment Construction

[0024] The use of molecular marker technology such as SSR to search for markers linked to late bolting genes provides an extremely useful tool for locating and cloning these genes, and lays a good foundation for molecular marker-assisted breeding. Molecular marker-assisted selection is not restricted by environmental conditions, and can realize seedling selection, reduce workload, and speed up the breeding process. At present, there are few studies on molecular markers of late bolting genes in non-heading Chinese cabbage at home and abroad.

[0025] Implementation procedure of the present invention is:

[0026] (1) Materials and methods The late bolting line Y5 of non-heading Chinese cabbage, that is, Shanghai Siyueman and early bolting line P120, that is, Nanjing Aijiaohuang, (see references: Cao Shouchun, Li Shijun. Preliminary Research on Local Varieties of Chinese Cabbage II. Main Research on biological characteristics. Journal of Nanjing Agricultural University, 1981, (1...

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Abstract

The present invention relates to a molecule marking method for a non-head Chinese cabbage late bolting gene, and the method belongs to the molecular biology field. Using the marking primer DBC16 to amplify the DNA of the non-head Chinese cabbage late bolting family or the breeding material, when the late bolting LB fragment of 252bp is amplified out, the existence of the non-head Chinese cabbage late bolting gene is proved. The present invention sieves the non-head Chinese cabbage late bolting family and the early bolting family P120 for the SSR molecule marking, and a late bolting mark LB ofthe non-head Chinese cabbage late bolting gene is obtained. The molecule marking provided by the present invention can be carried out for selecting and breeding the non-head Chinese cabbage late bolting breed, the select efficiency can be improved greatly, the breeding process can be accelerated, and in producing, the present invention can be applied to the purity identifying for the non-head Chinese cabbage late bolting breed.

Description

1. Technical field [0001] The invention relates to a molecular marker method for late bolting genes of non-heading Chinese cabbage, belongs to the field of molecular biology, relates to molecular breeding of late-bolting lines of non-heading Chinese cabbage, and is specially used for screening and identification of late-bolting gene varieties of non-heading Chinese cabbage. 2. Technical background [0002] Non-heading Chinese cabbage (Brassica campestris.ssp.chinensis.Makino), also known as Chinese cabbage, green vegetables, and rapeseed, is a vegetable cultivated in four seasons in the middle and lower reaches of the Yangtze River and its south. In recent years, it has also grown in a large area in northern my country. introduction, cultivation and promotion. However, due to early bolting, its yield and quality are significantly reduced, so late bolting has become an important breeding target for non-heading Chinese cabbage. [0003] Hidetoshi et al. used the BSA (bulked se...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 侯喜林张波史公军王建军
Owner 南京农业大学资产经营有限公司
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