Development and application of KASP marker of oryza sativa high-temperature-resistant gene TT1

A high-temperature, rice-resistant technology, applied in the field of genetic biology, can solve the problems of unsuitable high-throughput molecular detection platform, aerosol pollution environment, low detection efficiency, etc. easy effect

Inactive Publication Date: 2022-01-14
LIANYUNGANG ACAD OF AGRI SCI
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  • Abstract
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  • Claims
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Problems solved by technology

Linked molecular markers have also been developed for the above genes. However, although SSR markers, InDel markers or CAPS markers can be used for molecular marker-assisted selection, the detection efficiency is low, and aerosol pollution may also be generated, which is not suitable for high-throughput Molecular detection platform

Method used

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  • Development and application of KASP marker of oryza sativa high-temperature-resistant gene TT1
  • Development and application of KASP marker of oryza sativa high-temperature-resistant gene TT1
  • Development and application of KASP marker of oryza sativa high-temperature-resistant gene TT1

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Experimental program
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Effect test

Embodiment 1

[0045] Development of KASP Marker Tightly Linked to Rice High Temperature Tolerance Gene TT1

[0046] The development process of the KASP marker closely linked to the rice high temperature tolerance gene TT1 is as follows: figure 1 shown. According to literature reports, a SNP locus closely linked with TT1 gene was obtained.

[0047] 1. Determination of primers: The tightly linked SNP site of the TT1 gene was anchored at base 15422542 of rice chromosome 3 (Hu Xuejiao, Cheng Can, Tu Rongjian, et al. Development of functional markers for rice high temperature resistant TT1 gene and Application [J]. Molecular Plant Breeding, 2019, v.17(22):148-153.). The flanking sequence of closely linked SNP sites of TT1 gene was downloaded from NCBI database, and three sets of KASP primers were designed by using Primer5.0 software. After detection by the ArrayTape platform of Douglas Scientific Company, a set of KASP primers with good polymorphism was selected for subsequent verification. T...

Embodiment 2

[0072] Application of KASP marker closely linked to rice high temperature tolerance gene TT1 in marker-assisted selection of high temperature resistant rice plants

[0073] In order to test the practicability of the KASP_TT1 site of the present invention, the F1 population was obtained by crossing the high-temperature-resistant rice material CG14 with the non-high-temperature-resistant rice material Mianhui 725, and 48 F2 natural segregation populations were generated by natural selfing of F1, and the segregation populations were marked with KASP For detection and phenotypic verification (Table 2), refer to Example 1 for implementation methods of marker detection and phenotypic verification. Through the detection and analysis of phenotype and genotype of the isolated population, only 4 of the 48 individual plants of the isolated population were inconsistent with the genotype and phenotype results, and the consistency result between the marker KASP_TT1 and field resistance was P...

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Abstract

The invention discloses development and application of a KASP marker of an oryza sativa high-temperature-resistant gene TT1, and belongs to the technical field of gene biology. The molecular marker is a KASP marker KASP-TT1 which is closely linked with an oryza sativa high-temperature-resistant gene TT1, the KASP-TT1 is an SNP site in an oryza sativa genome, the nucleotide type of the KASP-TT1 is A or G, and the KASP-TT1 is the 101 nucleotide of SEQ ID No.4 in a sequence table. The KASP technology is applied to perform genotype identification on the oryza sativa high-temperature-resistant gene TT1, the method has the advantages of simplicity and convenience in operation, low cost, short detection period, stability in marking, environment friendliness and the like, the oryza sativa high-temperature-resistant gene TT1 can be accurately detected, and the method has important significance in promoting high-temperature-resistant oryza sativa breeding.

Description

technical field [0001] The invention relates to the development and application of the KASP marker of rice high temperature resistant gene TT1, and belongs to the field of gene biotechnology. Background technique [0002] Rice (Oryza sativa) is an important food crop in my country, and its planting area accounts for 30% of the total food crop planting area. Rice is not only the staple food of our people, but also an important raw material for industrial processing in our country. It is often used to make starch, wine, vinegar and medicine. Sex [J]. Agricultural Science and Technology Weekly, 2014, 005: 112-112.). Therefore, rice is of great strategic significance to my country's people's livelihood security, and rice production stability and increase is an important direction of research in our country. [0003] In recent years, high temperature heat damage has become an important natural disaster affecting rice production, and the middle and lower reaches of the Yangtze R...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11A01H1/04
CPCC12Q1/6895A01H1/04C12Q2600/13C12Q2600/156
Inventor 迟铭邢运高李景芳孙志广徐波刘艳刘晓敏王宝祥
Owner LIANYUNGANG ACAD OF AGRI SCI
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