Method of chrysanthemum amenone form molecular marker assistant selection

A technology of osmanthus osmanthus flower type and molecular markers, which is applied in the direction of biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve the problem of being easily affected by the environment and the small contribution rate of phenotypic variation of osmanthus osmanthus related flower traits , Difficult molecular marker-assisted selection, etc., to achieve the effect of wide selection range, early selection, and accelerated breeding process

Active Publication Date: 2018-08-17
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

In recent years, Chen Sumei et al. (2015) obtained some molecular markers related to flower organ traits of Tuogui-type chrysanthemum through linkage mapping method, but the markers were screened by the environment to 4 pairs of main effect QTLs that were less affected by the environment and had a greater contribution rate. and its related mo

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  • Method of chrysanthemum amenone form molecular marker assistant selection
  • Method of chrysanthemum amenone form molecular marker assistant selection
  • Method of chrysanthemum amenone form molecular marker assistant selection

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Embodiment 1

[0072] (1) Materials and methods

[0073] In this experiment, the cut-flower chrysanthemum 'Nannong Xuefeng' of the osmanthus-supporting type was used as the female parent, and the cut-flowering chrysanthemum 'QX096' was used as the male parent (such as figure 1 shown). In the autumn of 2014, the hybridization test was carried out, and 80 grains of F 1 The hybrid seeds were used for follow-up research, and the above materials were preserved in the "China Chrysanthemum Germplasm Resources Preservation Center" of Nanjing Agricultural University. In early March of the following year, they were planted on-demand in plug trays, together with the parent cutting seedlings, were planted in the chrysanthemum nursery in the middle and late April of 2015 and 2016, and the routine management was the same as that in the field. Observation and recording of parental and F 1 The flower type traits of the generation plants were screened for molecular markers and linkage analysis. The form...

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Abstract

The invention belongs to the technical field of biology and discloses a chrysanthemum amenone form molecular marker assistant selection method. A molecular marker is an SRAP molecular marker or/and SCAR marker; in the method, 80 F1 segregating populations, acquired by hybridizing amenone form spray cut chrysanthemum variety Nannong Xuefeng as a female plant and non amenone form variety QX096 as amale plant, are taken as a test material; specific sites, linked to amenone form genes, are screened and controlled by using the SRAP molecular marker, wherein SRAP molecular marker primer combinationM11E1 is amplified in an amenone form chrysanthemum material to obtain a specific fragment of 272bp; by designing a specific SCAR molecular marker primer, amplification is performed in an amenone material to obtain a single band of 168bp; it is further verified in two populations of Nannong Xuefeng*QX096 and Nannong Xuefeng*Mengbai that the accuracy reaches up to 92.5 percent and 84.3 percent respectively; and the situation that the marker is successfully transformed into a SCAR molecular marker is explained. The method provided by the invention improves the selection efficiency of an amenoneform marker and accelerates the selecting process of a new variety of the amenone form chrysanthemum.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for assisted selection of chrysanthemum and osmanthus-like molecular markers. Background technique [0002] Chrysanthemum (Chrysanthemum morifolium) is native to my country. It is one of the top ten traditional famous flowers in my country and one of the four major cut flowers in the world. It occupies an important position in the flower industry (Dai Silan, 2004). Tuogui is one of the important flower types of chrysanthemums. Its main characteristics are: the central tubular flowers are particularly developed, the tubular florets are elongated, and the corolla tube at the top is cracked like a supported osmanthus (Chen et al., 2009). Intogui-type chrysanthemums have unique flower shapes, and the tubular flowers and tongue-shaped flowers can show different colors. They have extremely high ornamental value and economic value, and are widely acclaimed. In recent y...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张飞仰小东苏江硕王海滨房伟民陈素梅陈发棣
Owner NANJING AGRICULTURAL UNIVERSITY
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