Brassica campestris ssp. Chinensis male sterile molecular marker-assisted selection method

A molecular marker-assisted, head-heading cabbage technology, applied in the directions of biochemical equipment and methods, DNA/RNA fragments, microorganism determination/inspection, etc. long effect

Active Publication Date: 2007-03-28
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

But so far there is no report on mtDNA cytoplasmic male sterility gene marker screening and marker-assisted selection of non-heading Chinese cabbage. MAS will improve the selection efficiency, thereby speeding up the breeding process

Method used

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  • Brassica campestris ssp. Chinensis male sterile molecular marker-assisted selection method
  • Brassica campestris ssp. Chinensis male sterile molecular marker-assisted selection method

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Embodiment Construction

[0031] Implementation procedure of the present invention is:

[0032] (1) Materials and methods Non-heading Chinese cabbage cytoplasmic male sterile line 98HA and its maintainer line 98HB, a near-isogenic line, were obtained from the Vegetable Research Institute of Nanjing Agricultural University (Hou X.L.Cao S.C and He Y.K.Creation of a new germplasm of CMS non -heading Chinese cabbage. Acta Horticulturae, 2004, 1: 75-81, the Vegetable Research Institute of Nanjing Agricultural University guarantees to provide it to the outside world within 20 years from the date of the patent application). Fertility investigation, screening of molecular markers and linkage analysis were carried out on the testcross and self-bred progenies of the cytoplasmic male sterile line 98HA and its maintainer line 98HB. The formula for calculating the recombination rate is: recombination rate = number of recombined plants / total number of plants × 100% .

[0033] (2) Molecular marker screening and analys...

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Abstract

The invention relates to a non-heading cabbage male sterile molecule marking assistant selecting method. Using marking primer of OPAG20 or SCAG20 to amplify non-heading cabbage male sterile series or breeding material chondriosome DNA, if the 700bp section is amplified, the male sterile gene is existed. The invention could be used to taking selecting to PAPD and SCAR molecule marking for non-heading cabbage male sterile series 98HA and the maintainer line 98HB chondriosome DNA to improve selecting efficiency and speed up breeding process.

Description

1. Technical field [0001] The invention discloses a non-heading cabbage male sterile molecular marker-assisted selection method, which belongs to the field of biotechnology and is used for the molecular marker-assisted selection breeding of non-heading Chinese cabbage cytoplasmic male sterile lines. 2. Technical background [0002] Non-heading cabbage (Brassica campestris ssp.chinensis Makino), native to China, is the most popular vegetable widely grown in southern my country, and plays a pivotal role in the annual supply of vegetables (Hou Xilin, 2003). Non-heading Chinese cabbage is a typical cross-pollination crop, with serious self-fertilization decline, and the yield advantage of excellent hybrid combinations is obvious. Non-heading Chinese cabbage has small flower organs and high cost of artificial detasseling. The use of male sterile lines is an ideal material for hybrid production. Among them, Cytoplasmic Male Sterility (CMS) can obtain 100% sterile lines. It has a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11C12P19/34
Inventor 侯喜林史公军
Owner NANJING AGRICULTURAL UNIVERSITY
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