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Ground cover chrysanthemum strain type stoloniferous numerator mark auxiliary selection method

A molecular marker-assisted and molecular marker technology, applied in the biological field, can solve the problems that molecular markers have not been reported yet, and achieve the effect of high intensity, long cycle and early selection

Inactive Publication Date: 2009-02-18
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
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  • Application Information

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Problems solved by technology

[0003] At present, research on plant type genetic control and molecular markers is mainly focused on the model plant Arabidopsis thaliana, field crops such as rice, wheat, corn, and vegetable crops such as tomato and kidney bean (Ji Yi et al., 2006; Zhang Peitong et al., 2006; Li Peijin et al. , 2003), there are few studies on plant type genetics and molecular markers of ornamental plants, but the molecular markers of ground cover chrysanthemum plant type creeping have not been reported yet

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  • Ground cover chrysanthemum strain type stoloniferous numerator mark auxiliary selection method
  • Ground cover chrysanthemum strain type stoloniferous numerator mark auxiliary selection method
  • Ground cover chrysanthemum strain type stoloniferous numerator mark auxiliary selection method

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Embodiment Construction

[0032] (1) Materials and methods

[0033] The test materials are the ground cover chrysanthemum variety 03(6)-12 and the potted small chrysanthemum variety 'Zao Italian Red' and 'Zao Italian Red' (P1) as the female parent , 03(6)-12 is the male parent (P2) and 152 strains including erect, creeping and intermediate F 1 Plant type segregation groups. Adopt the method of surveying branch angle (the mensuration of branch angle is carried out in seedling stage, measure the angle of first-level main branch extension direction and ground plane with protractor, get average value. Branch angle is divided into 9 grades, 0~ 10° is grade 1, 11-20° is grade 2, and so on, 81-90° is grade 9. The specified branch angles are in the range of grades 1, 2, and 3 as creeping plant types; grades 4, 5, and 6 It is an intermediate type; grades 7, 8, and 9 are erect plant types) to identify the plant type of a single plant, and perform molecular marker screening and linkage analysis. The formula for...

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Abstract

The invention relates to an auxiliary selecting method of a ground-cover chrysanthemum plant type creeping molecule mark, which belongs to the technical field of biology and which can be used for auxiliary selecting and breeding for the ground-cover chrysanthemum plant type creeping molecule mark. RAPD primer A-10 or an SCAR primer is used to augmentground-cover chrysanthemum or the breeding material DNA; if the fragment of 555bp can be augmented, the existence of a ground-cover chrysanthemum creeping is proofed. The method takes 152 plants of F1 segregation population obtained by taking 'early red Italy' (P1) as a female parent and takes 03(6)-12 as a male parent (P2) as the testing materials to build a plant type creeping / vertical gene pool to carry out screening on the RAPD and SCAR molecule marks, which can effectively develop the auxiliary breeding for the ground-cover chrysanthemum plant type creeping molecule mark and greatly improve the selecting efficiency, thereby quickening the breeding process.

Description

1. Technical field [0001] The invention discloses a molecular marker-assisted selection method for ground cover chrysanthemum plant type creeping property, which belongs to the field of biotechnology and is used for molecular marker assisted selection breeding of ground cover chrysanthemum plant type creeping property. 2. Background technology [0002] Ground cover chrysanthemum is a new species of chrysanthemum first proposed by Academician Chen Junyu in the 1980s (Wang Pengwei et al., 1990; Chen J Y et al.1995). It has the characteristics of low plant type or creeping, multi-branching, strong resistance, wide adaptability, fast forming, strong covering ability, dense flowering, etc., and has comprehensive functions of greening, beautification, colorization and fragrance, and has a very broad application prospect in gardens (Yu Zhongxiang, 2004). Plant type is one of the important traits of ground cover chrysanthemum, and creeping ground cover chrysanthemum is more suitabl...

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Application Information

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IPC IPC(8): C12Q1/68C12N15/29
Inventor 陈发棣赵静媛陈素梅缪恒彬崔新利姜贝贝房伟民管志勇
Owner NANJING AGRICULTURAL UNIVERSITY
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