Kit and method for quantitative detection of 25-hydroxyvitamin D through TRFIA

Abstract

The invention relates to a 25-hydroxyvitamin D TRFIA (Time-Resolved Fluoroimmunoassay) kit and a detection method, which utilize the sensitivity of TRFIA technology, utilize rare earth vanadate nano fluorescent labeling materials and combine a dry-type immunofluorescence analyzer to realize high sensitivity, accurate quantification, simpleness and convenience. The kit of the invention can accurately and quantitatively detect the content of the 25-hydroxyvitamin D in serum, plasma and whole blood samples of persons, the whole blood samples can be filtered through the treatment of blood filtering membrane sample pads, the fluorescence interference of the samples can be avoided by utilization of a time resolution detection technology, the fluorescent microspheres are rare earth vanadate nanomaterials, thus the advantages of low background, strong fluorescence signals, high signal-to-noise ratio and the like are achieved, rare earth vanadate nano particles are connected with antibody through covalent bonds by labelling, a labeled product is stable, thus the kit has the characteristics of wide detection range (3-100ng / mL), high sensitivity (detection limit is 3ng / mL), high accuracy, quick and convenient detection and the like.

Description

[0001] (1) Technical field [0002] The invention relates to a kit and a detection method for time-resolved fluorescence quantitative detection of 25-hydroxyvitamin D. [0003] (2) Background technology [0004] Vitamin D is a fat-soluble steroid precursor, mainly produced by the skin after exposure to light. Two important forms of vitamin D include vitamin D 3 (cholecalciferol) and vitamin D 2 (ergocalciferol), in humans, vitamin-binding protein and vitamin D 3 and vitamin D 2 Combined and transported to the liver, the two are hydroxylated to produce 25-hydroxyvitamin D. 25-hydroxyvitamin D is a metabolite that can be detected in blood, and most of the 25-hydroxyvitamin D detected in serum is 25-hydroxyvitamin D3, so the status of vitamin D can be determined by detecting 25-OH VD . Vitamin D can promote intestinal absorption of calcium and regulate calcium balance, and is the main element for maintaining bone health. Severe vitamin D deficiency in childhood can lead to s...

AI Technical Summary

Problems solved by technology

[0007] In addition, most of the 25-OH VD in the blood is combined with the vitamin-binding protein VDBP. Therefore, a sample dissociation solution is required to dissociate 25-OH VD from VDBP for measurement. Most of the existing dissociation methods are insufficiently dissociated and affect the measurement results.

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