Molecular marker for co-separating from aroma character

A molecular marker and co-separation technology, applied in the field of molecular genetics, can solve the problems of rice aroma genes that have not been reported, and achieve the effects of speeding up assisted breeding, saving costs, and stabilizing markers.

Inactive Publication Date: 2010-12-22
SHANDONG RICE RES INST
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Problems solved by technology

[0007] The technology of molecular marker-assisted selection of rice aroma genes using molecular markers has been reported at home and abroad, but so far, there has been no report on the co-segregation markers of rice aroma genes, and the use of TPS solution to identify aroma traits is also the first report.

Method used

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  • Molecular marker for co-separating from aroma character
  • Molecular marker for co-separating from aroma character
  • Molecular marker for co-separating from aroma character

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Embodiment Construction

[0031] 1. F 2 Population creation and phenotyping

[0032] In 2005, Qufu fragrant rice, Mingshui fragrant rice, and Xiangjing 9407 were crossed with IRBB60. At the end of the year, F 1 The seeds are sent to Sanya, Hainan Island for generation, self-bred to produce F 2 seed. 2006 will F 2 The separated populations were sown on the experimental farm of Shandong Rice Research Institute. They were sown on May 1 and seedlings were planted on June 20. Single plant was planted. Planting seedlings for 20 days to extract F 2 Isolate the DNA of individual plants of the population, and use the method for identifying rice aroma of the present invention (TPS method) to identify the aroma traits of individual rice plants. There are two phenotypes of aroma and no aroma. Each isolated population planted 3000 plants, totaling 9000 plants .

[0033] 2. F 2 Molecular Marker Analysis of Segregated Population

[0034] (1) Using CTAB method to extract F from Qufu Fragrant Rice / IRBB60, Mingshui Fragrant ...

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Abstract

The invention relates to a molecular marker for co-separating from aroma characters, belonging to the field of molecular genetics. 1-2 pieces of spires at the upper part of individual rice are taken at the tillering stage and are reserved in a refrigerator with the temperature of below 80 DEG C for later use. Rice leaves with the length of 2-4cm are taken to be placed in a centrifugal tube of 1.5ml, liquid nitrogen is added, grinding is carried out, 900 mu l of TPS solution is added, and water bath is performed for 20min at the temperature of 75 DEG C. A cover is opened to smell the odour by a nose to determine whether aroma exists. A specific PCR primer L01 or L02 is utilized to obtain the molecular marker by PCR amplification. The invention can be specially used for seed selection and genetic research of the rice aroma variety and can be used for cloning aroma genes.

Description

[0001] This application is a divisional application of the original application number 200810015264.9, the filing date is April 18, 2008, and the invention title is "a method for identifying the aroma of rice and a molecular marker co-separated with the aroma traits". Technical field [0002] The invention relates to a molecular marker co-separated with fragrance traits, which belongs to the field of molecular genetics, and is dedicated to the breeding and genetic research of rice fragrance varieties, and can be used to clone fragrance genes. Background technique [0003] Aroma is an important eating quality trait of rice. Many excellent rice varieties have a pleasant aroma and are popular with consumers. Fragrant rice is grown in every rice producing country or region in the world. [0004] At present, the main methods for determining aroma include chewing grain method, potassium hydroxide method, and high performance liquid chromatography. The first two methods are carried out by ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 姚方印李广贤姜明松李润芳张晓东王文英
Owner SHANDONG RICE RES INST
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