38 results about "Pyricularia grisea" patented technology

The invention belongs to the technical field of gene engineering, and provides application of a magnaporthe oryzae Pmk1 protein K347 site as a target for inhibiting the pathogenicity of magnaporthe oryzae. Researches find that after the K347 site of the magnaporthe oryzae Pmk1 protein is mutated, the pathogenicity of magnaporthe oryzae is remarkably reduced, growth and expansion of magnaporthe oryzae hyphae can be inhibited, autophagy in the conidium germination process can be promoted, and secretion of effect protein of magnaporthe oryzae can be inhibited. Compared with the drug design aiming at the whole Pmk1 gene, the drug design aiming at the K347 site disclosed by the invention is accurate in range and has very high specificity and biological safety. The invention provides a new target site for developing a pyricularia grisea antibacterial drug, and the site has good application prospect and value in the aspect of preventing and controlling the rice blast.

The invention discloses application of polypeptide compounds in prevention and treatment of pathogenic microorganisms. The compound has a synergistic effect on agricultural fungi such as rhizoctonia solani, Sclerotinia sclerotiorum, Botrytis cinerea, Fusarium graminearum, Pyricaria oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae and pyricularia oryzae. The method comprises the following steps: carrying out sterilization on plant bacteria such as Xanthomonas oryzae PXO99A, Xanthomonas axonophora dispsp.citrijx-6, Pseudomonas solanacearum (Smith) BNCC335855, Pseudomonas sp. The method is characterized by comprising the following steps of: killing Aspergillus flavus ACCC32601 (Aspergillus flavus), Penicillium expansum ACCC 30898 (Penicillium expansum. EXPANSum ACCC 30898), Monilinia coerulea ACCC 36263 (Aspergillus oryzae, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger and Aspergillus niger); the compound disclosed by the invention has a certain inhibition activity on various diseases caused by streptococcus agalactiae ATCC 27956, streptococcus dysgalactiae ATCC27957, Escherichia coli ATCC 25922 and Staphylococcus aureus, particularly, the compound Thiostreptin has a particularly remarkable inhibition effect on agricultural pathogenic bacteria, food-borne germs and cow mastitis germs, and is expected to be developed into a novel antibacterial drug, so that the compound disclosed by the invention can be used for preparing the antibacterial agent for preventing and treating various diseases caused by the streptococcus agalactiae ATCC 27956, the streptococcus dysgalactiae ATCC27957, the Escherichia coli ATCC 25922 and the Staphylococcus aureus.

The invention belongs to the field of crop genetic breeding, and particularly relates to a rice blast resistant gene OsK8 (Os04g0658700 gene) and application thereof. The invention discloses a rice blast resistance gene OsK8. The nucleotide sequence of the gene is shown as SEQ ID NO: 1. The invention also discloses application of the rice blast-resistant gene OsK8. The OsK8 gene is knocked out from rice, so that the resistance of rice plants to pyricularia oryzae can be effectively enhanced; and the gene Os04g0658700 is overexpressed in the rice, so that the resistance of the rice to the pyricularia grisea is reduced.

The invention belongs to the field of microorganisms, and particularly relates to a banana endophyte compound fungicide as well as a preparation method and application thereof. According to the invention, a novel endophytic bacillus velezensis JDB15 strain and an endophytic trichoderma harzianum JDL4 strain are separated from a banana plant. The trichoderma harzianum JDL4 can inhibit botrytis cinerea, fusarium graminearum, rhizoctonia solani, peach anthracnose pathogen, peach brown rot pathogen and alternaria alternata, and has a good broad-spectrum antibacterial effect. Meanwhile, after the filtrate of the fermentation liquor of the trichoderma harzianum JDL4 and the bacillus velezensis JDB15 are compounded, the antibacterial effect is remarkably improved, and the control effect on banana fusarium wilt, pyricularia oryzae, tomato fusarium wilt and corn bipolaris maydis is also remarkably improved. The compound bacterial agent has wide biocontrol bacterial activity, can be developed deeply subsequently, and provides a sustainable and green solution for prevention and treatment of banana wilt and other plant diseases.

The invention discloses trichosporon and application thereof, and relates to the technical field of phytopathogen prevention and control. The trichosporon sp. Is named as trichosporon sp., the strain number of the trichosporon sp. Is ZJU2023TR1, and the preservation number of the trichosporon sp. Is CCTCC (China Center for Type Culture Collection) NO: M 20231367. The trichosporon sp. ZJU2023TR1 provided by the invention can inhibit the growth of pyricularia grisea and corn southern leaf blight pathogenic bacteria in a plate confrontation test; in a fermentation liquor test, the inhibition effect on magnaporthe oryzae reaches up to 87.65%, and the inhibition effect on corn southern leaf blight pathogenic bacteria reaches up to 91.58%. Therefore, the strain ZJU2023TR1 can effectively inhibit the growth of the pyricularia grisea and the corn southern leaf blight pathogenic bacteria, can be used for preparing a novel, efficient and broad-spectrum biocontrol inoculant, and provides a new way for the development of microorganisms applied to biological control.

The invention discloses a rice OsCMPG1 gene and application thereof in rice disease resistance, and belongs to the technical field of molecular biological agriculture. The gene OsCMPG1 is knocked out through a CRISPR / Cas9 technology, and a knockout mutant oscmpg1 is obtained; it is found through rice blast resistance identification that compared with wild type rice, the mutant oscmpg1 is more resistant to diseases and small in disease spot area, the number of pyricularia grisea carried by diseased leaves is small, and it is indicated that the OsCMPG1 negatively regulates the resistance of rice to rice blast. The bacterial leaf blight resistance identification finds that compared with wild rice, the mutant oscmpg1 is more resistant to diseases and shorter in scabs, which indicates that the OsCMPG1 negatively regulates the resistance of the rice to the bacterial leaf blight.

The invention relates to the technical field of biological prevention and control, in particular to a method for analyzing rice leaf rhythm change after rice is infected by pyricularia grisea and application of the method in accurate prevention and control of rice blast. The analysis method comprises the following steps: (1) spraying a pyricularia grisea spore suspension on rice leaves; (2) carrying out whole-genome transcriptome analysis on the rice leaves, and carrying out clustering heat map; (3) analyzing expression conditions of the biological clock genes and the phytochrome related genes in the circadian rhythm pathway of the rice leaves, and analyzing the biological clock genes and the phytochrome related genes by a MetaCycle method; and (4) analyzing the deposition amount of H2O2 and callose in the rice leaves. Determining the dynamic change rule of the rice immune process caused by magnaporthe oryzae infection according to the method; in combination with the characteristics of small molecules, small-molecule pesticides are reasonably applied at a specific time point aiming at the rice blast, so that efficient and accurate prevention and control of the rice blast are realized.

The invention discloses a broussonetia papyrifera source ecological pesticide or growth promoting agent and a preparation process thereof.The broussonetia papyrifera leaf extract is prepared from broussonetia papyrifera leaves, ethyl alcohol, dichloromethane, ethyl acetate and n-butyl alcohol, and the preparation process of the broussonetia papyrifera source ecological pesticide or growth promoting agent comprises the following steps that broussonetia papyrifera leaf raw materials are prepared; extracting a paper mulberry leaf raw material by using alcohol, adding petroleum ether to extract the solution collected in the step 2, collecting the obtained solution, adding dichloromethane to the solution collected in the step 3, adding ethyl acetate to the solution collected in the step 4, adding n-butyl alcohol to the solution collected in the step 5, collecting the obtained extract, and drying to obtain the paper mulberry leaf extract. Purifying the final extract of the broussonetia papyrifera leaves to prepare a pesticide or a growth promoting agent. According to the tree source ecological pesticide or the growth promoting agent and the preparation process thereof, the pesticide or the growth promoting agent is prepared from the extracts of the paper mulberry leaves, so that the germination of appressorium of pyricularia grisea spores of rice blast can be inhibited, the effect of promoting the growth of rice is achieved, and the yield of the rice is increased.

The invention discloses bacillus velezensis as well as a biological preparation and application thereof, and belongs to the technical field of microorganisms. The bacillus velezensis is rice endophytic bacillus velezensis, is classified and named as bacillus velezensis HA-B13, is preserved in the China Center for Type Culture Collection (CCTCC), has the preservation number of CCTCC NO: M 20251098, shows extremely excellent biological activity to nigatosporium oryzae, has the antibacterial rate of 94%, and can be used for preparing the bacillus velezensis. Meanwhile, the bacillus subtilis also has relatively good biological activity on a plurality of rice pathogenic bacteria such as rhizoctonia solani, pyricularia grisea, gylastoma gonoryzae and the like. And the strain is derived from rice plants and can be well colonized in the rice plants, so that the strain has a long-acting prevention and treatment effect on diseases such as spike rot and the like, can effectively reduce the investment of rice disease prevention and treatment products, is safer and has great prevention and treatment application potential.

The invention discloses a long-chain non-coding RNA (Ribonucleic Acid) gene OslncBR3 and application of the long-chain non-coding RNA gene OslncBR3 to improvement of rice blast resistance. The nucleotide sequence of the long-chain non-coding RNA gene OslncBR3 is as shown in SEQ ID NO. 1 (sequence identifier number 1). The invention discovers and proves that the long-chain non-coding RNA OslncBR3 positively regulates the rice blast resistance for the first time, the OslncBR3 is knocked out by a genetic engineering method to reduce the resistance of rice to pyricularia grisea, and the OslncBR3 is overexpressed to significantly enhance the resistance of rice to pyricularia grisea. The invention can make up the defects of the existing rice disease-resistant long-chain non-coding RNA research, and can improve the rice blast resistance caused by magnaporthe oryzae when being applied to rice, thereby accumulating valuable gene resources and theoretical basis for the breeding of new rice blast-resistant varieties.

The application discloses application and a method of a small peptide GLP-1(28-36)amide in preparation of a medicine for preventing and treating rice blast. A scheme discloses application of the small peptide GLP-1(28-36)amide in preparation of a medicine for preventing and treating Pyricularia oryzae, and application of the provided small peptide GLP-1(28-36)amide in inhibiting Pyricularia oryzae and preventing and treating rice blast. The small peptide GLP-1(28-36)amide of the application can effectively inhibit pathogenicity of spores of Pyricularia oryzae in a concentration range of 12.5-100 muM, in particular, the small peptide GLP-1(28-36)amide with a concentration of 100 muM can completely prevent and treat rice blast, and can be used for developing a new type of green pesticide.

The invention discloses a substituted quinoline compound and a preparation method and application thereof.The preparation method includes the steps that thionyl chloride and a specific intermediate react, DCM and other solvents are adopted, and pyridine, polyphosphoric acid, ethyl alcohol, acetyl chloride and other reagents are used in the reaction process. The structure of the obtained product is confirmed by a nuclear magnetic hydrogen spectrum, bactericidal activity tests are carried out on the obtained 29 target products, and the results show that the compound has a significant inhibition effect on a variety of plant pathogens at a concentration of 50 ppm, and especially shows excellent bactericidal activity in the aspects of pyricularia grisea, sclerotinia sclerotiorum and the like. In addition, the compounds have certain herbicidal activity.

The invention discloses a 3-ester-1, 3-quinazoline-2, 4-diketone compound and a preparation method and application thereof.The 3-ester-1, 3-quinazoline-2, 4-diketone compound is prepared by the steps that amino-acid ester hydrochloride and isatoic anhydride serve as raw materials and react in acetonitrile under the action of potassium carbonate to generate an amide ester compound; and then reacting the amide ester compound with triphosgene under the action of triethylamine to obtain the 3-ester-1, 3-quinazoline-2, 4-diketone compound. Raw materials for synthesis of the compound are cheap and easy to obtain, the synthesis method is simple, meanwhile, the compound has good bacteriostatic activity on crop germs, especially has a remarkable activity inhibition effect on germs such as gibberellic disease bacteria, phytophthora germs, pyricularia grisea, sclerotinia sclerotiorum, botrytis cinerea and rhizoctonia solani, and the yield of crops is well guaranteed.

The invention discloses pseudomonas aeruginosa, which is pseudomonas aeruginosa EB-Q03-02, the preservation number of the pseudomonas aeruginosa EB-Q03-02 is CGMCC (China General Microbiological Culture Collection Center) No.30779, the preservation number of the pseudomonas aeruginosa EB-Q03-02 is CGMCC No. 30779, and the preservation number of the pseudomonas aeruginosa EB-Q03-02 is CGMCC No. 30779. The pseudomonas aeruginosa is applied to prevention and treatment of pyrenospora cinerea, helminthosporium planhilum and phytophthora nicotianae. The pseudomonas aeruginosa EB-Q03-02 is separated from a healthy passion fruit root system, has an antagonistic effect on the passion fruit epidemic disease, and particularly has an obvious antagonistic effect on preventing and treating the passion fruit epidemic disease caused by phytophthora nicotianae; furthermore, the pseudomonas aeruginosa EB-Q03-02 disclosed by the invention can be used for effectively inhibiting pyrenospora cinerea, helminthosporium planhilum and phytophthora nicotianae, and the inhibition rate is up to 81% or above.

The invention belongs to the technical field of immune anti-attractants, and particularly relates to an immune anti-attractant containing probenazole and a preparation method of the immune anti-attractant. The immune anti-attractant containing probenazole is prepared from the following raw materials in parts by mass: 10 to 15 parts of probenazole, 0.01 to 0.10 part of sedaxane, 0.01 to 0.10 part of validamycin, 0.1 to 2 parts of a binding agent, 0.1 to 2 parts of a dispersing agent, 0.1 to 2 parts of a wetting agent, 50 to 80 parts of calcium carbonate and 20 to 30 parts of water. The immune anti-attractant containing probenazole disclosed by the invention has a relatively good disease-resistant effect on three diseases of rice damping-off, rice blast and banded sclerotial blight at the same time, and is high in heat storage stability at the same time.

The invention provides a preparation method and application of methyl 2-hydroxyphenylacetate. The methyl 2-hydroxyphenylacetate is separated from a fermentation culture of streptomyces wetland 13-3 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 22830. According to the method, the compound methyl 2-hydroxyphenylacetate is obtained from streptomycete for the first time, verification proves that the methyl 2-hydroxyphenylacetate has broad-spectrum antibacterial activity on various plant pathogenic fungi, particularly, the inhibitory activity on aspergillus fumigatus, colletotrichum truncatum, rhizoctonia solani, verruca verrucosa and magnaporthe oryzae is higher than that of amphotericin B, and the methyl 2-hydroxyphenylacetate can be used for preparing antibacterial agent drugs and has broad-spectrum antibacterial activity. A novel natural antibacterial compound is provided for the fields of agriculture and medicine, and the method is simple and convenient to operate, high in purification efficiency and environment-friendly.

The invention discloses application of a rice OsSIT1 gene in regulation and control of resistance of rice to rice blast. The CDS nucleotide sequence of the rice OsSIT1 gene disclosed by the invention is as shown in SEQ ID NO. 1. Researches show that the rice OsSIT1 gene plays an important role in the rice blast resistance process of rice. The deletion of the rice OsSIT1 gene reduces the resistance of the rice to the pyricularia grisea physiological race RB22, so that the rice OsSIT1 gene has important significance for cultivating rice blast resistant rice plants, and is suitable for popularization and application.

The invention belongs to the technical field of plant disease and insect pest control, and particularly relates to biocontrol pseudomonas fluorescens FH and application thereof, and the preservation number of the pseudomonas fluorescens FH is CCTCC NO: M 2025096. The pseudomonas fluorescens FH not only can effectively inhibit pyricularia grisea, but also can promote germination of the millet seeds, and the strain can promote germination of the millet seeds on the premise that normal growth of crops is not affected, so that the dual effects of resisting diseases and promoting growth are achieved. A new technical approach is provided for solving the problem of valley blast, and efficient, economical, safe and environment-friendly prevention and control are achieved.

The invention discloses application of salinomycin to prevention and treatment of pathogenic microorganisms. According to the present invention, the compound provides significant inhibition activity for agricultural fungi such as Sclerotinia sclerotiorum, Botrytis cinerea, Pyricaria oryzae, Moniliniafacticola ACCC 36263, food-borne bacteria such as Escherichia coli O157: H7ATCC 35150, Bacillus cereus BN CC 103930, Listeria monocytogenes ATCC 19115 and Salmonella enteritidis ATCC 14028, and further provides a certain inhibition effect on other agricultural pathogenic bacteria, such that the compound is expected to be developed into the one type of the compound,

The invention belongs to the technical field of plant disease control, and provides application of a tetrahydro-gamma-carboline derivative. The structural formula of the tetrahydro-gamma-carboline derivative is shown in the specification. The derivative has a good inhibition effect on fungal diseases of rice and tobacco and can be used for preparing pesticides for preventing and treating tobacco target leaf spot bacteria, alternaria alternata, rhizoctonia solani and pyricularia grisea, and a new parent structure is provided for development of the pesticides. The two tetrahydro-gamma-carboline derivatives provided by the invention can also be compounded with various types of pesticides, have a good synergistic effect on the plant diseases, and can be used for delaying the resistance development of existing commercial medicaments and prolonging the life cycle.

The invention discloses a rice gene OsLTD1 and application thereof. The CDS sequence of the rice gene OsLTD1 is as shown in SEQ ID NO.1, and the amino acid sequence of encoding protein of the rice gene OsLTD1 is as shown in SEQ ID NO.3. The invention further discloses a preparation method of the rice gene OsLTD1. Through gene localization and function complementation verification technologies, it is identified that function deletion of the OsLTD1 gene can significantly enhance the resistance of rice to rice blast. The function deletion mutant osltd1 of the gene shows leaf yellowing and lesion-like phenotypes from a seedling stage to a tillering stage, and shows broad-spectrum resistance to a plurality of pyricularia grisea physiological races. According to the invention, the broad-spectrum disease-resistant character and the excellent agronomic character are efficiently combined through a visual leaf color marker associated with gene function deletion, a new high-yield and disease-resistant rice variety is finally cultivated, and a new technical scheme and a new gene resource are provided for solving the problem of rice blast prevention and treatment.

The application discloses a Burkholderia glumae RS-36 and application thereof, and belongs to the technical field of microorganisms. Burkholderia gladioli The application provides a Burkholderia glumae (B. glumae) RS-36 with a preservation number of CCTCC No: M 20221584, and application of the B. glumae RS-36. Burkholderia gladioli The B. glumae RS-36 provided by the application has inhibiting effects on Magnaporthe oryzae, Ustilaginoidea virens, Exserohilum turcicum and Pyricularia grisea, can secrete indole acetic acid for promoting plant growth, can keep good activity in an acidic environment with a pH of 5 and a drought environment, has strong stress resistance, and has important significance for promoting green and sustainable development agriculture.

The invention relates to a fluorine-containing nitrogen heterocyclic compound, which is a series of compounds with a structure as shown in a formula I: # imgabs0 #. The novel series of fluorine-containing nitrogen heterocyclic compounds provided by the invention have excellent biological activity on various plant pathogenic fungi. The bactericidal composition has excellent prevention and treatment effects on diseases caused by botrytis cinerea, rhizoctonia solani, wheat stem rot, physalospora piricola, pyricularia grisea, sigatoka and the like, is safe to crops, and has great popularization and application values.

The invention discloses a thifluzamide and trifluoropyridinamine compounded bactericide composition and application thereof, and belongs to the technical field of agricultural bactericides. According to the technical scheme, the bactericidal composition comprises effective components and auxiliary components, the effective components are thifluzamide and trifluoropyridinamine, the mass ratio of thifluzamide to trifluoropyridinamine is (1-10): (1-10), and the bactericidal composition has prevention and treatment effects on banded sclerotial blight, damping off and stem rot caused by rhizoctonia and botrytis cinerea, powdery mildew, colletotrichum gloeosporioides, alternaria alternata and magnaporthe oryzae. Through scientific proportioning, the composition disclosed by the invention not only can expand the prevention and treatment range, but also can realize a synergistic or synergistic effect, effectively reduce the dosage of a single agent, delay the generation of resistance and improve the pesticide effect durability and application safety, so that the requirements of agricultural production on efficient, safe and environment-friendly bactericides are better met; good popularization and application values are realized.

The invention discloses application of a quinine derivative in prevention and treatment of agricultural pathogenic bacteria, and relates to the technical field of medicinal chemistry. A biological activity test shows that the quinine derivative has a significant effect on plant pathogenic bacteria such as Xanthomonas oryzae pv. Oryzae, Xanthomonas citri pv. Citri and tomato bacterial wilt, and plant pathogenic fungi such as rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, magnaporthe oryzae, fusarium pseudograminearum and fusarium graminearum. And the compound shows certain inhibitory activity on decay-causing pathogenic fungi such as aspergillus flavus, penicillium expansum and brown rot of stone fruits, and has a relatively good inhibitory effect on pathogenic bacteria of rice bacterial blight and rice blast bacteria. The compound is low in preparation difficulty, raw materials are cheap and easy to obtain, and the compound is expected to be developed into a novel agricultural antibacterial agent.

The invention discloses application of a rice endogenous oligopeptide in inducing rice to resist rice blast. A rice gene Bphi008a is knocked out by adopting CRISPR / Cas9, pyricularia grisea living injury inoculation experiments are respectively carried out on wild type and Bphi008a gene knockout mutant plants, and the result shows that the Bphi008a gene knockout mutant plants show a susceptible phenotype to pyricularia grisea, which indicates that the gene positively regulates the resistance of rice to pyricularia grisea. Furthermore, an oligopeptide containing 39 amino acids is designed and synthesized on the basis of the encoding protein of the Bphi008a gene. Oligopeptide with the final concentration of 10 mu g / mL is exogenously added into rice seedlings, and expression of rice resistance related PR genes (OsPR1a, OsPR5, OsPR10a and OsPAL06) can be quickly activated after the rice seedlings are treated by the ligopeptide for 6 hours. After the oligopeptide is treated for 24 hours, pyricularia oryzae is inoculated by spraying, and the application of the oligopeptide can obviously enhance the resistance of the rice to the pyricularia oryzae. In addition, 100 [mu] g / mL of short peptide has an obvious inhibition effect on the growth of bacterial blight. The results show that the oligopeptide has an application value of being developed into a novel biological resistance inducer and a bacteriostatic agent.

The invention discloses an application of a small peptide GLP-1 (28-36) amide in preparation of a medicine for preventing and treating rice blast and a method of the small peptide GLP-1 (28-36) amide. The invention discloses an application of a small peptide GLP-1 (28-36) amide in preparation of a medicine for preventing and treating magnaporthe oryzae, and provides an application of the small peptide GLP-1 (28-36) amide in inhibition of magnaporthe oryzae and prevention and treatment of magnaporthe oryzae. The small peptide GLP-1 (28-36) amide can effectively inhibit pathogenicity of pyricularia oryzae spores in the concentration range of 12.5-100 [mu] M, especially the small peptide GLP-1 (28-36) amide with the concentration of 100 [mu] M can completely prevent and treat pyricularia oryzae, and the small peptide GLP-1 (28-36) amide can be used for developing novel green pesticides.

The invention belongs to the technical field of microorganisms, and particularly relates to defensive pseudomonas JR-4 and application thereof in preventing and treating rice blast. The pseudomonas defensive JR-4 provided by the invention has the preservation number of CCTCC NO: M 20252405, is separated from rhizosphere soil of dry land high-quality rice-Yunnan rice you 615 in a healthy land parcel adjacent to a rice blast occurrence land parcel, has the capabilities of fixing nitrogen, dissolving cellulase, dissolving phosphorus and producing siderophores, can effectively inhibit rice blast bacteria, and has a good application prospect. Especially, hypha growth of pyricularia oryzae with strong pathogenicity can be effectively inhibited, and the bacteriostasis rate reaches 46.71%. Moreover, results of in-vitro leaf inoculation and root irrigation tests show that the fermentation broth for preventing the pseudomonas JR-4 provided by the invention can effectively inhibit the occurrence of the rice blast, and has good prevention and treatment effects on the rice blast of the rice, especially the rice variety of Yunnan rice Yunyou 615.

The invention discloses application of spiramerin in improving resistance of rice to rice blast and / or banded sclerotial blight, and relates to the technical field of biology. According to the method, endogenous metabolites capable of influencing the resistance of rice to the rice blast are excavated, the influence of the endogenous metabolites on the disease resistance of the rice is identified in the field through three treatment modes of simultaneous treatment, prevention treatment and treatment, and the result shows that the spiramerin can remarkably enhance the resistance of the rice to the rice blast. The result shows that after spiramerin treatment, pyricularia grisea spore germination and appressorium formation are inhibited. In addition, it is also found that spiramerin has prevention and treatment effects on rice sheath blight disease. The invention provides a novel endogenous metabolite for preventing and treating rice blast and sheath blight, and has important agricultural application value.

The invention discloses a screening method of magnaporthe oryzae toxin degrading bacteria, and relates to the technical field of microbiological screening, the method comprises the following specific steps: sample collection and strain preliminary separation: collecting a plurality of samples in different rice field environments, and obtaining pure culture strains through a dilution coating method or a tissue isolation method through multiple times of streak separation; primary screening and HPLC secondary screening based on a culture medium: preparing a culture medium primary screening strain by taking the magnaporthe oryzae toxin as a unique carbon and nitrogen source, through the culture medium primary screening by taking the magnaporthe oryzae toxin as the unique carbon and nitrogen source, potential degradation strains can be rapidly enriched from numerous microorganisms, a large amount of invalid screening is avoided, and the degradation capability of the strains can be accurately determined through HPLC secondary screening; according to the method, efficient degradation strains are quickly locked, target strains are further screened by utilizing the infection relation between bacteriophages and germs through phage-mediated screening, the overall screening efficiency is remarkably improved, the screening period is greatly shortened, and the strains with the capacity of efficiently degrading the pyricularia oryzae toxins are obtained more quickly.