27 results about "Pyricularia grisea" patented technology

The invention belongs to the field of microorganisms, and particularly relates to a banana endophyte compound fungicide as well as a preparation method and application thereof. According to the invention, a novel endophytic bacillus velezensis JDB15 strain and an endophytic trichoderma harzianum JDL4 strain are separated from a banana plant. The trichoderma harzianum JDL4 can inhibit botrytis cinerea, fusarium graminearum, rhizoctonia solani, peach anthracnose pathogen, peach brown rot pathogen and alternaria alternata, and has a good broad-spectrum antibacterial effect. Meanwhile, after the filtrate of the fermentation liquor of the trichoderma harzianum JDL4 and the bacillus velezensis JDB15 are compounded, the antibacterial effect is remarkably improved, and the control effect on banana fusarium wilt, pyricularia oryzae, tomato fusarium wilt and corn bipolaris maydis is also remarkably improved. The compound bacterial agent has wide biocontrol bacterial activity, can be developed deeply subsequently, and provides a sustainable and green solution for prevention and treatment of banana wilt and other plant diseases.

The invention relates to the technical field of biological prevention and control, in particular to a method for analyzing rice leaf rhythm change after rice is infected by pyricularia grisea and application of the method in accurate prevention and control of rice blast. The analysis method comprises the following steps: (1) spraying a pyricularia grisea spore suspension on rice leaves; (2) carrying out whole-genome transcriptome analysis on the rice leaves, and carrying out clustering heat map; (3) analyzing expression conditions of the biological clock genes and the phytochrome related genes in the circadian rhythm pathway of the rice leaves, and analyzing the biological clock genes and the phytochrome related genes by a MetaCycle method; and (4) analyzing the deposition amount of H2O2 and callose in the rice leaves. Determining the dynamic change rule of the rice immune process caused by magnaporthe oryzae infection according to the method; in combination with the characteristics of small molecules, small-molecule pesticides are reasonably applied at a specific time point aiming at the rice blast, so that efficient and accurate prevention and control of the rice blast are realized.

The invention discloses bacillus velezensis as well as a biological preparation and application thereof, and belongs to the technical field of microorganisms. The bacillus velezensis is rice endophytic bacillus velezensis, is classified and named as bacillus velezensis HA-B13, is preserved in the China Center for Type Culture Collection (CCTCC), has the preservation number of CCTCC NO: M 20251098, shows extremely excellent biological activity to nigatosporium oryzae, has the antibacterial rate of 94%, and can be used for preparing the bacillus velezensis. Meanwhile, the bacillus subtilis also has relatively good biological activity on a plurality of rice pathogenic bacteria such as rhizoctonia solani, pyricularia grisea, gylastoma gonoryzae and the like. And the strain is derived from rice plants and can be well colonized in the rice plants, so that the strain has a long-acting prevention and treatment effect on diseases such as spike rot and the like, can effectively reduce the investment of rice disease prevention and treatment products, is safer and has great prevention and treatment application potential.

The invention discloses a long-chain non-coding RNA (Ribonucleic Acid) gene OslncBR3 and application of the long-chain non-coding RNA gene OslncBR3 to improvement of rice blast resistance. The nucleotide sequence of the long-chain non-coding RNA gene OslncBR3 is as shown in SEQ ID NO. 1 (sequence identifier number 1). The invention discovers and proves that the long-chain non-coding RNA OslncBR3 positively regulates the rice blast resistance for the first time, the OslncBR3 is knocked out by a genetic engineering method to reduce the resistance of rice to pyricularia grisea, and the OslncBR3 is overexpressed to significantly enhance the resistance of rice to pyricularia grisea. The invention can make up the defects of the existing rice disease-resistant long-chain non-coding RNA research, and can improve the rice blast resistance caused by magnaporthe oryzae when being applied to rice, thereby accumulating valuable gene resources and theoretical basis for the breeding of new rice blast-resistant varieties.

The application discloses application and a method of a small peptide GLP-1(28-36)amide in preparation of a medicine for preventing and treating rice blast. A scheme discloses application of the small peptide GLP-1(28-36)amide in preparation of a medicine for preventing and treating Pyricularia oryzae, and application of the provided small peptide GLP-1(28-36)amide in inhibiting Pyricularia oryzae and preventing and treating rice blast. The small peptide GLP-1(28-36)amide of the application can effectively inhibit pathogenicity of spores of Pyricularia oryzae in a concentration range of 12.5-100 muM, in particular, the small peptide GLP-1(28-36)amide with a concentration of 100 muM can completely prevent and treat rice blast, and can be used for developing a new type of green pesticide.

The invention discloses a substituted quinoline compound and a preparation method and application thereof.The preparation method includes the steps that thionyl chloride and a specific intermediate react, DCM and other solvents are adopted, and pyridine, polyphosphoric acid, ethyl alcohol, acetyl chloride and other reagents are used in the reaction process. The structure of the obtained product is confirmed by a nuclear magnetic hydrogen spectrum, bactericidal activity tests are carried out on the obtained 29 target products, and the results show that the compound has a significant inhibition effect on a variety of plant pathogens at a concentration of 50 ppm, and especially shows excellent bactericidal activity in the aspects of pyricularia grisea, sclerotinia sclerotiorum and the like. In addition, the compounds have certain herbicidal activity.

The invention discloses a 3-ester-1, 3-quinazoline-2, 4-diketone compound and a preparation method and application thereof.The 3-ester-1, 3-quinazoline-2, 4-diketone compound is prepared by the steps that amino-acid ester hydrochloride and isatoic anhydride serve as raw materials and react in acetonitrile under the action of potassium carbonate to generate an amide ester compound; and then reacting the amide ester compound with triphosgene under the action of triethylamine to obtain the 3-ester-1, 3-quinazoline-2, 4-diketone compound. Raw materials for synthesis of the compound are cheap and easy to obtain, the synthesis method is simple, meanwhile, the compound has good bacteriostatic activity on crop germs, especially has a remarkable activity inhibition effect on germs such as gibberellic disease bacteria, phytophthora germs, pyricularia grisea, sclerotinia sclerotiorum, botrytis cinerea and rhizoctonia solani, and the yield of crops is well guaranteed.

The invention belongs to the technical field of immune anti-attractants, and particularly relates to an immune anti-attractant containing probenazole and a preparation method of the immune anti-attractant. The immune anti-attractant containing probenazole is prepared from the following raw materials in parts by mass: 10 to 15 parts of probenazole, 0.01 to 0.10 part of sedaxane, 0.01 to 0.10 part of validamycin, 0.1 to 2 parts of a binding agent, 0.1 to 2 parts of a dispersing agent, 0.1 to 2 parts of a wetting agent, 50 to 80 parts of calcium carbonate and 20 to 30 parts of water. The immune anti-attractant containing probenazole disclosed by the invention has a relatively good disease-resistant effect on three diseases of rice damping-off, rice blast and banded sclerotial blight at the same time, and is high in heat storage stability at the same time.

The invention provides a preparation method and application of methyl 2-hydroxyphenylacetate. The methyl 2-hydroxyphenylacetate is separated from a fermentation culture of streptomyces wetland 13-3 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 22830. According to the method, the compound methyl 2-hydroxyphenylacetate is obtained from streptomycete for the first time, verification proves that the methyl 2-hydroxyphenylacetate has broad-spectrum antibacterial activity on various plant pathogenic fungi, particularly, the inhibitory activity on aspergillus fumigatus, colletotrichum truncatum, rhizoctonia solani, verruca verrucosa and magnaporthe oryzae is higher than that of amphotericin B, and the methyl 2-hydroxyphenylacetate can be used for preparing antibacterial agent drugs and has broad-spectrum antibacterial activity. A novel natural antibacterial compound is provided for the fields of agriculture and medicine, and the method is simple and convenient to operate, high in purification efficiency and environment-friendly.

The invention belongs to the technical field of plant disease and insect pest control, and particularly relates to biocontrol pseudomonas fluorescens FH and application thereof, and the preservation number of the pseudomonas fluorescens FH is CCTCC NO: M 2025096. The pseudomonas fluorescens FH not only can effectively inhibit pyricularia grisea, but also can promote germination of the millet seeds, and the strain can promote germination of the millet seeds on the premise that normal growth of crops is not affected, so that the dual effects of resisting diseases and promoting growth are achieved. A new technical approach is provided for solving the problem of valley blast, and efficient, economical, safe and environment-friendly prevention and control are achieved.

The invention belongs to the technical field of plant disease control, and provides application of a tetrahydro-gamma-carboline derivative. The structural formula of the tetrahydro-gamma-carboline derivative is shown in the specification. The derivative has a good inhibition effect on fungal diseases of rice and tobacco and can be used for preparing pesticides for preventing and treating tobacco target leaf spot bacteria, alternaria alternata, rhizoctonia solani and pyricularia grisea, and a new parent structure is provided for development of the pesticides. The two tetrahydro-gamma-carboline derivatives provided by the invention can also be compounded with various types of pesticides, have a good synergistic effect on the plant diseases, and can be used for delaying the resistance development of existing commercial medicaments and prolonging the life cycle.

The invention discloses a rice gene OsLTD1 and application thereof. The CDS sequence of the rice gene OsLTD1 is as shown in SEQ ID NO.1, and the amino acid sequence of encoding protein of the rice gene OsLTD1 is as shown in SEQ ID NO.3. The invention further discloses a preparation method of the rice gene OsLTD1. Through gene localization and function complementation verification technologies, it is identified that function deletion of the OsLTD1 gene can significantly enhance the resistance of rice to rice blast. The function deletion mutant osltd1 of the gene shows leaf yellowing and lesion-like phenotypes from a seedling stage to a tillering stage, and shows broad-spectrum resistance to a plurality of pyricularia grisea physiological races. According to the invention, the broad-spectrum disease-resistant character and the excellent agronomic character are efficiently combined through a visual leaf color marker associated with gene function deletion, a new high-yield and disease-resistant rice variety is finally cultivated, and a new technical scheme and a new gene resource are provided for solving the problem of rice blast prevention and treatment.

The application discloses a Burkholderia glumae RS-36 and application thereof, and belongs to the technical field of microorganisms. Burkholderia gladioli The application provides a Burkholderia glumae (B. glumae) RS-36 with a preservation number of CCTCC No: M 20221584, and application of the B. glumae RS-36. Burkholderia gladioli The B. glumae RS-36 provided by the application has inhibiting effects on Magnaporthe oryzae, Ustilaginoidea virens, Exserohilum turcicum and Pyricularia grisea, can secrete indole acetic acid for promoting plant growth, can keep good activity in an acidic environment with a pH of 5 and a drought environment, has strong stress resistance, and has important significance for promoting green and sustainable development agriculture.

The invention discloses a thifluzamide and trifluoropyridinamine compounded bactericide composition and application thereof, and belongs to the technical field of agricultural bactericides. According to the technical scheme, the bactericidal composition comprises effective components and auxiliary components, the effective components are thifluzamide and trifluoropyridinamine, the mass ratio of thifluzamide to trifluoropyridinamine is (1-10): (1-10), and the bactericidal composition has prevention and treatment effects on banded sclerotial blight, damping off and stem rot caused by rhizoctonia and botrytis cinerea, powdery mildew, colletotrichum gloeosporioides, alternaria alternata and magnaporthe oryzae. Through scientific proportioning, the composition disclosed by the invention not only can expand the prevention and treatment range, but also can realize a synergistic or synergistic effect, effectively reduce the dosage of a single agent, delay the generation of resistance and improve the pesticide effect durability and application safety, so that the requirements of agricultural production on efficient, safe and environment-friendly bactericides are better met; good popularization and application values are realized.

The invention discloses application of a quinine derivative in prevention and treatment of agricultural pathogenic bacteria, and relates to the technical field of medicinal chemistry. A biological activity test shows that the quinine derivative has a significant effect on plant pathogenic bacteria such as Xanthomonas oryzae pv. Oryzae, Xanthomonas citri pv. Citri and tomato bacterial wilt, and plant pathogenic fungi such as rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, magnaporthe oryzae, fusarium pseudograminearum and fusarium graminearum. And the compound shows certain inhibitory activity on decay-causing pathogenic fungi such as aspergillus flavus, penicillium expansum and brown rot of stone fruits, and has a relatively good inhibitory effect on pathogenic bacteria of rice bacterial blight and rice blast bacteria. The compound is low in preparation difficulty, raw materials are cheap and easy to obtain, and the compound is expected to be developed into a novel agricultural antibacterial agent.

The invention discloses application of a rice endogenous oligopeptide in inducing rice to resist rice blast. A rice gene Bphi008a is knocked out by adopting CRISPR / Cas9, pyricularia grisea living injury inoculation experiments are respectively carried out on wild type and Bphi008a gene knockout mutant plants, and the result shows that the Bphi008a gene knockout mutant plants show a susceptible phenotype to pyricularia grisea, which indicates that the gene positively regulates the resistance of rice to pyricularia grisea. Furthermore, an oligopeptide containing 39 amino acids is designed and synthesized on the basis of the encoding protein of the Bphi008a gene. Oligopeptide with the final concentration of 10 mu g / mL is exogenously added into rice seedlings, and expression of rice resistance related PR genes (OsPR1a, OsPR5, OsPR10a and OsPAL06) can be quickly activated after the rice seedlings are treated by the ligopeptide for 6 hours. After the oligopeptide is treated for 24 hours, pyricularia oryzae is inoculated by spraying, and the application of the oligopeptide can obviously enhance the resistance of the rice to the pyricularia oryzae. In addition, 100 [mu] g / mL of short peptide has an obvious inhibition effect on the growth of bacterial blight. The results show that the oligopeptide has an application value of being developed into a novel biological resistance inducer and a bacteriostatic agent.

The invention discloses an application of a small peptide GLP-1 (28-36) amide in preparation of a medicine for preventing and treating rice blast and a method of the small peptide GLP-1 (28-36) amide. The invention discloses an application of a small peptide GLP-1 (28-36) amide in preparation of a medicine for preventing and treating magnaporthe oryzae, and provides an application of the small peptide GLP-1 (28-36) amide in inhibition of magnaporthe oryzae and prevention and treatment of magnaporthe oryzae. The small peptide GLP-1 (28-36) amide can effectively inhibit pathogenicity of pyricularia oryzae spores in the concentration range of 12.5-100 [mu] M, especially the small peptide GLP-1 (28-36) amide with the concentration of 100 [mu] M can completely prevent and treat pyricularia oryzae, and the small peptide GLP-1 (28-36) amide can be used for developing novel green pesticides.

The invention belongs to the technical field of microorganisms, and particularly relates to defensive pseudomonas JR-4 and application thereof in preventing and treating rice blast. The pseudomonas defensive JR-4 provided by the invention has the preservation number of CCTCC NO: M 20252405, is separated from rhizosphere soil of dry land high-quality rice-Yunnan rice you 615 in a healthy land parcel adjacent to a rice blast occurrence land parcel, has the capabilities of fixing nitrogen, dissolving cellulase, dissolving phosphorus and producing siderophores, can effectively inhibit rice blast bacteria, and has a good application prospect. Especially, hypha growth of pyricularia oryzae with strong pathogenicity can be effectively inhibited, and the bacteriostasis rate reaches 46.71%. Moreover, results of in-vitro leaf inoculation and root irrigation tests show that the fermentation broth for preventing the pseudomonas JR-4 provided by the invention can effectively inhibit the occurrence of the rice blast, and has good prevention and treatment effects on the rice blast of the rice, especially the rice variety of Yunnan rice Yunyou 615.

The invention discloses a screening method of magnaporthe oryzae toxin degrading bacteria, and relates to the technical field of microbiological screening, the method comprises the following specific steps: sample collection and strain preliminary separation: collecting a plurality of samples in different rice field environments, and obtaining pure culture strains through a dilution coating method or a tissue isolation method through multiple times of streak separation; primary screening and HPLC secondary screening based on a culture medium: preparing a culture medium primary screening strain by taking the magnaporthe oryzae toxin as a unique carbon and nitrogen source, through the culture medium primary screening by taking the magnaporthe oryzae toxin as the unique carbon and nitrogen source, potential degradation strains can be rapidly enriched from numerous microorganisms, a large amount of invalid screening is avoided, and the degradation capability of the strains can be accurately determined through HPLC secondary screening; according to the method, efficient degradation strains are quickly locked, target strains are further screened by utilizing the infection relation between bacteriophages and germs through phage-mediated screening, the overall screening efficiency is remarkably improved, the screening period is greatly shortened, and the strains with the capacity of efficiently degrading the pyricularia oryzae toxins are obtained more quickly.

The invention discloses a carboline alkaloid compound and application thereof in prevention and treatment of agricultural pathogenic bacteria, and relates to the technical field of natural medicinal chemistry. The carboline alkaloid compound disclosed by the invention can be used for preventing and treating agricultural fungal diseases caused by agricultural fungi such as rhizoctonia solani, Sclerotinia sclerotiorum, Botrytis cinerea, Fusarium graminearum and Pyricaria oryzae, and can also be used for preventing and treating plant bacterial diseases caused by plant bacteria such as Xanthomonas oryzae ACCC 11602 and Xanthomonas axonopois pv.citri. The compound disclosed by the invention can be used for preventing and treating the plant bacterial diseases caused by the plant bacteria such as the Xanthomonas oryzae and the Xanthomonas axonopois pv.citri. As a novel agricultural bactericide, the carboline alkaloid compound has the characteristics of novel structure, broad spectrum and high efficiency, and has the value of being further researched and developed into the novel agricultural bactericide.

The utility model provides a field collection device for pyricularia grisea, and belongs to the technical field of agricultural collection. The pyricularia grisea field collecting device comprises a collecting box and a collecting and slitting mechanism, a collecting cover is installed on the rear side of the collecting box, a fixing strip is installed on the rear side of the right side of the collecting box, slitting grooves are formed in the two sides of the inner wall of the collecting cover, and slitting knives located in the slitting grooves are fixedly connected to the two sides of the rear side of the collecting box. The turnover grooves are formed in the two sides of the rear side of the fixing strip, turnover strips located in the turnover grooves are fixedly connected to the two sides of the front side of the right side of the collecting cover, and by arranging the collecting and slitting mechanism, in the process that a worker samples diseased plants, closed slitting work can be conducted on the positions of sampled pyricularia oryzae; the sampling device is simple in structure and convenient to use, workers can rapidly sample diseased plants, the situation that germ spores fly in the diseased plant sampling process is prevented, and therefore the sampling convenience and the sampling effect of the rice blast germs are improved.

The invention discloses application of a magnaporthe oryzae squalene cyclooxygenase gene MoERG1 in prevention and treatment of magnaporthe oryzae, and belongs to the technical field of agricultural biology. The invention proves that the squalene cyclooxygenase gene MoERG1 of the magnaporthe oryzae is a key essential gene for environmental adaptability and pathogenicity of the magnaporthe oryzae. The gene is specifically silenced by an RNA interference technology, so that the completeness of cell walls of pathogenic bacteria can be effectively damaged, the tolerance of the pathogenic bacteria to oxidative stress and hypertonic stress is remarkably weakened, the yield of conidia and the formation rate of a key infection structure-appressorium of the pathogenic bacteria are greatly reduced, and finally, the pathogenic ability of the pathogenic bacteria to rice is completely lost. The core function of the squalene ring oxygenase gene MoERG1 as a multi-effect regulation target is disclosed from the molecular level. In order to solve the industrial problems that the existing single-target bactericide is easy to generate drug resistance and the traditional biocontrol effect is unstable, the invention provides a brand-new effective solution with a more fundamental action mechanism.

The application discloses an arecoline ester derivative, a preparation method and application thereof, relates to the technical field of chemical pesticides, and has a brand-new molecular structure, and the bacteriostatic activity and insecticidal activity of the arecoline ester derivative are significantly enhanced compared with arecoline; the arecoline ester derivative has certain inhibiting effects on Puccinia recondita, Diplodia natalensis, Gibberella saubinetii, Pyricularia grisea, Phomopsis vexans, Physalospora piricola, Pestalotiopsis versiflorea, and Colletotrichum gloeosporioides; and the arecoline ester derivative also has certain insecticidal activity on Aedes aegypti larvae.

The invention provides a method for enhancing plant disease resistance based on rice disease-resistant PTR protein and application. The rice disease-resistant PTR protein is an endoplasmic reticulum positioned pentamer Ca < 2 + > channel, the C terminal of the rice disease-resistant PTR protein comprises a self-inhibition structural domain, the structural domain contains a CaaX motif (CCIS), and C902-site cysteine is subjected to farnesylation modification so as to maintain the inactivation state of the channel. According to the present invention, the pyricularia grisea effect protein AVR-Pita specifically recognizes and cuts the site (PEPPA / KPEP) between the 874th alanine (A874) and the 875th lysine (K875) of the PTR, and a self-inhibition module is removed so as to activate the Ca < 2 + > channel activity, release the Ca < 2 + > of the endoplasmic reticulum to the cytoplasm, and further trigger the immune response; the method can be widely applied to disease-resistant breeding of rice and other crops, and has good application prospects and industrial value.

The application discloses a bacillus licheniformis longus agent, a preparation method and application, and the agent is prepared from a bacillus licheniformis longus strain C1 and comprises the following steps: culturing the bacillus licheniformis longus strain through a tryptone soy agar culture medium until yellow single colonies appear; inoculating the yellow single colonies into serum, and performing seed culture on the bacillus licheniformis longus liquid culture medium until a logarithmic growth phase is reached to obtain a bacterial liquid; inoculating the cultured bacterial liquid into a production device according to the bacillus licheniformis longus strain, and performing production culture on the bacillus licheniformis longus production culture medium until a logarithmic growth phase is reached to prepare a bacillus licheniformis longus biocontrol agent; performing centrifugal treatment on the obtained bacillus licheniformis longus biocontrol agent, taking supernatant, and performing filtration on the supernatant to prepare a bacillus licheniformis longus biocontrol fermentation liquid. The bacillus licheniformis longus agent is applied to antagonizing pyricularia oryzae and preventing and controlling rice blast.

The invention relates to the field of plant disease control, and discloses a siderophore antifungal agent and application thereof in plant disease control. The siderophore antifungal agent disclosed by the invention is prepared from the following components: (2R, 4R)-2-[(4R)-2-(2-hydroxyphenyl)-4, 5-dihydro-1, 3-thiazole-4-yl]-3-methyl-1, 3-thiazolidine-4-carboxylic acid. When the siderophore antifungal agent is used for preventing and treating plant diseases, the formation of conidium appressorium can be inhibited, and the proportion of anthrax hijinensis appressorium which intrudes hyphae and infects flowering cabbage cotyledons is only 6% and is obviously lower than 86% of a control group. The disease index of the flowering cabbage plant inoculated with the colletotrichum hijinensis can be obviously reduced from 74 to 27 at the concentration of 10 mu M, and 0 mm disease spots can be realized at the concentration of 30 mu M; meanwhile, the compound also has a certain control effect on fungi such as magnaporthe oryzae and colletotrichum gloeosporioides, and shows broad-spectrum antibacterial activity.

The invention belongs to the technical field of microbial fertilizer manufacturing, and particularly relates to a rice nano bacterial fertilizer for reducing the incidence rate of rice blast. According to the nano bacterial fertilizer, corynespora cassiicola, coniothyrium minitans YAFEF037 and mucor are introduced, antibacterial substances can be generated, growth and reproduction of pyricularia oryzae can be inhibited, competitive advantages can be formed on rhizosphere and leaf surfaces of rice, ecological niches of pathogenic bacteria are occupied, and then the incidence rate of the pyricularia oryzae is effectively reduced; due to the high specific surface area and adsorbability of the nano silicon dioxide, a good adhesion and growth environment is provided for microorganisms, and the metabolic capability and stress resistance of the strain are further enhanced; the organic matter fermentation liquor contains a large amount of nutritional ingredients, growth and development of rice root systems can be promoted, and the nutrient absorption capacity of rice is improved; the growth vigor and the stress resistance of the rice are improved through the nano bacterial fertilizer, the resistance of the rice to the rice blast is further enhanced, and a synergistic effect mechanism of promoting the fertilizer with bacteria and preventing diseases with the fertilizer is formed.