88 results about "Pyricularia grisea" patented technology

The invention discloses bacillus subtilis NB12, as well as a culture method and an application thereof. The strain with a preservation number of CGMCC No.5383 is preserved in China General Microbiological Culture Collection Center on October 24, 2011. Researches prove that: the strain has strong inhibition effects to rhizoctonia solani, colletotrichum musae, pyricularia grisea, fusarium graminearum, fusarium oxysporum f.sp.cubense, peronophythora litchi, ustilago scitaminea, fulvia fulva, xanthomonas oryzae and xanthomonas oryzae pv.oryzicola, is a broad-spectrum antagonistic strain, can be used for prevention and control of various plant diseases, has a particular good prevention and control effect to the rice sheath blight and damping off in seedling phase of cowpea, has the advantages of harmlessness to human and livestock, greenness and safety, good environment compatibility, difficult resistance generation and the like, can be used for commercial development, and has a wide application prospect.

The invention discloses burkholderia cepacia SD7 and a culturing method and application thereof. The bacterial strain is preserved in China General Microbiological Culture Collection Center on October 24th, 2001, and the preservation number is CGMCC No.5384. Through research, the bacterial strain has strong inhibiting effect on rhizoctonia solani, collectotrichum musae, pyricularia grisea, gibberella zeae, fusarium oxysporum f. sp. cubense, peronophythora litchi, ustiago scitamimea syd, fulvia fulva, xanthomonas oryzaepv.oryzae, xanthomonas oryzae pv. Oryzicola, and the like, is broad-spectrum antagonistic bacteria, can be used for preventing and treating a plurality of plant diseases, particularly has better preventing and treating effect for banana fruit anthracnose and rice sheath blight, has the advantages of harmlessness to person and animals, environmental protection and safety, good environmental compatibility, and difficulty in becoming resistant, is good for business development, and has broad application prospect.

The invention discloses a strain of efficient bacillus amyloliquefaciens, and a bacterial agent and application thereof, wherein the Latin name of the bacillus amyloliquefaciens is Bacillus amyloliquefaciens; the strain number is HW05; the accession number of the bacillus amyloliquefaciens in China General Microbiological Culture Collection Center is CGMCC No. 10273. The efficient bacillus amyloliquefaciens provided by the invention has the obvious inhibition effects on fusarium oxysporum, phytophthora capsici, ralstonia solanacearum, pyricularia grisea, colletotrichum gloeosporioides and root-knot nematode. Through the single application of HW05, the efficient prevention and control effects can be achieved on leaf surface disease sweet melon powdery mildew, mango anthracnose diseases andmulberry red rust diseases; the HW05 and paecilomyces lilacinus can be matched for preventing and treating soil-borne banana wilting diseases and banana root-knot nematode diseases, and a good synergyeffect is achieved.

The invention discloses streptomyces katrae NB 20, as well as a culture method and an application thereof. The strain with a preservation number of CGMCC No.5382 is preserved in China General Microbiological Culture Collection Center on October 24, 2011. Researches prove that: the strain has strong inhibition effects to colletotrichum musae, pyricularia grisea, fusarium graminearum, fusarium oxysporum f.sp.cubense, peronophythora litchi, ustilago scitaminea, fulvia fulva, xanthomonas oryzae and xanthomonas oryzae pv.oryzicola, is a broad-spectrum antagonistic strain, can be used for prevention and control of various plant diseases, has a particular good prevention and control effect to the colletotrichum musae, has the advantages of harmlessness to human and livestock, greenness and safety, good environment compatibility, difficult resistance generation and the like, and has a wide application prospect.

The invention discloses a strain of plant pathogenic fungi antagonistic bacterium and the application of the plant pathogenic fungi antagonistic bacterium in preventing and curing plant diseases. The plant pathogenic fungi antagonistic bacterium is bacillus subtilis BEB2 CGMCC No.2243. The plant pathogenic fungi antagonistic bacterium bacillus subtilis BEB2 CGMCC No.2243 can prevent and cure the plant diseases which are caused by banana vascular wilt pathogen, colletotrichum musae, pyricularia grisea, corynespora leaf-fall disease of hevea rubber, bamboo fusarium axysporum and mango anthracnose pathogen, and the plant pathogenic fungi antagonistic bacterium bacillus subtilis BEB2 CGMCC No.2243 in particular can inter-grow with bananas, can survive in the bananas stably, and can prevent and cure banana panama diseases.

The invention discloses an oleanolic acid oxime ester derivate compound, a preparation method and an application thereof. The structural formula of the compound is shown in formula I, and in the formula I, R refers to furyl, pyridyl, 1-menaphthyl, 4-chlorophenoxy methyl or phenyl with 1 to 2 substitutional groups; and the substitutional groups in phenyl with 1 to 2 substitutional groups are halogen (such as fluorine, chlorine, bromine and iodine) or nitryl. The preparation method comprises the following steps: 1) performing the esterification reaction between oleanolic oxime subject to benzylation protection and substituted carboxylic acids to prepare an oxime ester compound; and 2) removing benzylation protection on the oxime ester compound by Pd / C-H2 to prepare the compound in the formula I. The bactericidal activity test result shows that CAU2012-A has a favorable effect of inhibiting growth of sclerotinia scleotiorum, phytophthora melongenae, botrytis cintrea, pyricularia grisea, rhizoctonia solani and cotton fusarium wilt.

The invention of molecular labeling method of Pyricularia grisea avirulent gene belongs to the field of agricultural biotechnology. The molecular labeling technology includes performing molecular labeling to avirulent gene though assessment from two parental strains and filial generation groups of Pyricularia grisea. M355-356 labeled by SSR near NO. 1 chromosome telomere links with avirulent gene Avr-Pit with a genetic distance of 2.3cm; S487 labeled by RAPD located near NO. 7 chromosome telomere links with avirulent gene Avr-Pia with a genetic distance of 3.5cm; S361 labeled by m677-678 and RAPD located at middle of N0. 3 chromosome telomere links with Pyricularia grisea specified avirulent gene PRE1 with genetic distances of 5.9cm and 6.4. The invention can not only make use of the nontoxic gene marker as gene probe, but also set foundations for further clone of the gene through physical mapping.

The invention provides a biological pesticide by using psoralidin to control plant fungous disease. The invention relates to a plant used biological pesticide, in particular to biological pesticide by using psoralidin to control plant fungous disease. Compound is dried Guzhi seed 5Kg. The compound is quenched and extracted four times by using 70 percent of industrial ethanol. After lixivium is decompressed and condensed, ethanol extract is gained. Water is added and mixed with the ethanol extract. Mixture is extracted by using petroleum ether and ethyl acetate in turn. 100g of ethyl acetate extraction part extract is taken, and processed chromatography over again by silica gel column, and processed gradient elution by petroleum ether-ethyl acetate and are separated, and then psoralidin (50mg) is gained (the ratio between the petroleum ether and ethyl acetate is 4 to 1). The invention can restrain common plant pathogen, like apple decay pathogen, apple macrophoma kawatsukai, pyricularia grisea, rice sheath blight fungus, cucumber fusarium wilt, wheat scab pathogen, cucumber anthracnose, phytophthora parasitica and so on.

The invention relates to a method for simply and rapidly identifying the resistance of the pyricularia grisea. The method comprises the steps of: extracting a heading rice plant from the rice field, respectively taking rice internode stems with lengths of not less than 5cm above and below an internode by using the internode of a spike neck for later use; placing degreasing cotton in a moisturizing plastic box, irrigating 8-10ml of spore suspension solution each milliliter of which contains 0.6 millions pyricularia grisea; and placing the rice internode stems to be detected on the degreasing cotton, moisturizing and cultivating for 14 days at a temperature of 25-26 DEG C so that scabs which can be fully identified can be formed, wherein sizes and invasion degrees of the scabs are divided into five levels, the rice internode stems with 0-level or 1-level morphological characteristics are of a disease-resistant type R, the rice internode stems with 4-level morphological characteristics are of a disease-susceptible type S, and the rice internode stems with 2-level or 3-level morphological characteristics are of a middle type M. Identification can be carried out indoors by using a stem node inoculating method, thus errors caused by identification under the natural condition in the field are avoided. The method does not need equipment, and is simple and easy to operate and strong in practicability.

The invention belongs to the field of biological pesticides, and discloses a sugarcane endophytic bacillus amyloliquefaciens RGB15 strain and an application thereof. The bacillus amyloliquefaciens CGB15 strain is preserved in the Guangdong Province Microbial Culture Collection Center on October 17, 2019, and the preservation number is GDMCC No: 60742, and the preservation address is the 5th floorof No.59 building of No.100 courtyard of the Xianlie Middle Road of Guangzhou city. The bacillus amyloliquefaciens RGB15 strain has a strong inhibition effect on ustilago scitaminea, pyricularia grisea, peronophythora litchii, colletotrichum litchii and banana fusarium oxysporum, and has an excellent biological control effect. A microbial preparation prepared from the strain is simple in preparation and use method, has broad-spectrum disease resistance, has little influence on the ecological environment, does not influence the crop quality, and has an excellent application prospect.

The invention discloses an application of pseudolaric acid B in preventing plant diseases. An experiment proves that the pseudolaric acid B has agricultural fungicidal activity. The compound has good bacteriostasis on setosphaeria turcica, pyricularia grisea, botrytis cinerea, rhizoctonia solani, mango stem end rot pathogen and colletotrichum gloeosporioides when the concentration for testing is 5mg / L, and the EC value is between 0.08mg / L-1mg / L; the compound has a certain spore germination inhibition on the setosphaeria turcica and the botrytis cinerea when the EC values are only 7.806mg / L and 4.104mg / L respectively; and at the same time, the result of a living body experiment shows that the compound further has good protective and therapeutic actions on the botrytis cinerea. The result indicates that the pseudolaric acid B has broad-spectrum bactericidal effect on the plant diseases, which lays the foundation for creating a novel non-polluted plant source pesticide by using the unique plant pseudolaric acid B in China.

The invention discloses an application of a volatile substance produced by streptomyces fimicarius in control of plant diseases. Particularly, the volatile substance produced by streptomyces fimicarius can inhibit growth of 15 phytopathogens, has the highest inhibition function on peronophythora litchii, pythium myriotylum, alternaria alternate, fusarium graminearum, colletotrichum musarum and pyricularia grisea and has the inhibition rate up to 100%. The invention further makes clear that the volatile substance produced by streptomyces fimicarius contains 7 compounds with remarkable bacteriostasis, and a direction is provided for future studies of control drugs for peronophythora litchii, pythium myriotylum, alternaria alternate, colletotrichum musarum, pyricularia grisea and other phytopathogens.

The invention relates to an application of an isoflavonoids compound, that is, glabridin, to prepare an agricultural bactericide. The compound is used for inhibiting the following pathogenic funguses of crops: fusarium graminearum, colletotrichum gloeosporioides, botrytis cinerea, phytophthora infestans, or pyricularia grisea, and the following pathogenic bacteria of crops: erwina carotovora, pseudomonas solanacearum, ralstonia solanacearum, or pseudomonas syringae pv. actinidae. Furthermore, the compound can be prepared into various formulations with other agricultural auxiliaries, and the compound has a synergistic effect after combined with shikonin according to a certain weight ratio.

The invention provides a set of production technology for preventing ralstonia solanacearum from growing and reproducing in solanaceae crops to increase crop yield by aiming at the defects of the prior art for controlling the damages of the ralstonia solanacearum to the crops. Brevibacillus choshinensis X23 has a wide bacteriostatic spectrum and good environmental safety and has antagonism effects to various pathogenic fungi, such as phytophthora parasitica var nicotianae, pyricularia grisea, alternaria alternate, phytophthora infestans and the like, so that the brevibacillus choshinensis X23 has favorable development prospect and application potential.

The invention discloses a pesticide composition containing oxine-copper and kasugamycin, and an application thereof. The composition comprises oxine-copper, kasugamycin and common assistants, and a weight ratio of the oxine-copper to the kasugamycin is (1-60 ):(1-20). The oxine-copper and the kaempromycin have significant synergistic effect after being compounded, and the doses of effective components of the above compounded medicament are obviously lower than those of individually used effective components, so the pesticide composition ahs the characteristics of synergistic effects, delaying of drug resistance, enlarged control range, good control effect, small dose and low agricultural cost. The composition is suitable for controlling most fungal diseases, and especially Pyricularia grisea, bacterial leaf streak, watermelon bacterial angular leaf spot disease and citrus ulcer disease.

The invention discloses an entomopathogenic nematode symbiotic bacteria strain for inhibiting various plant fungal diseases and application. The bacteria strain is a high-toxicity bacteria strain NN6of xenorhabdus bovienii, the preservation number is CCTCC NO: M 2020097, and the bacteria strain is preserved in China Center for Type Culture Collection on April 29, 2020. The xenorhabdus bovienii NN6 disclosed by the invention has relatively strong ability of inhibiting growth of fusarium oxysporum f.sp.niveum, fusarium oxysporium f.sp.cucumerinum, fusarium graminearum, pyricularia grisea, botrytis cinerea and phytophthora capsici hypha, the ability is increased along with the increase of volume concentration, and the antibacterial effect is obvious. The bacteria strain also has the effect of inhibiting the germination of fusarium spores, and the inhibition effect is remarkable. The bacteria strain has the characteristics of low culture condition requirement, long lasting period, environment-friendly and non-toxic source and the like, has important significance in protecting the ecological environment and improving the added value of agricultural products, and has good market development and application prospects due to the advantage of wider antibacterial spectrum.

The invention discloses a loquat endophytic unciform trichoderma strain and applications of the loquat endophytic unciform trichoderma strain. The endophytic trichoderma strain is obtained through separation, then morphological and molecular biological identification is carried out, meanwhile, the antibacterial activity of the endophytic trichoderma strain is tested, and thus a material is provided for the development and utilization of the strain; the endophytic trichoderma strain has the antibacterial activity for five phytopathogens, wherein the inhibiting effect for fungi is obviously stronger than that for bacteria, specifically, the inhibiting effect for pyricularia grisea is the strongest and is 83.17%, the antibacterial rate for alternaria tenuissima takes the second place and is 75.10%, the antibacterial rates for fusarium proliferatum and fusarium sambucinum have no obvious differences and are respectively 52.02% and 49.07%, and the antibacterial rate for Xanthomonas oryzae pv.oyzae is the poorest, and is 38.24%. The trichoderma strain has the antibacterial wide spectrum, and has the development and utilization values and prospects.

This invention encompasses the identification and isolation of genes that confer disease control properties in plants, as well as plants comprising such genes. These genes are derived from the following sources: Nicotiana benthamiana, Oryzae sativa (var. Indica IR7), Papaver rhoeas, Saccharomyces cerevisiae and Trichoderma harzianum (Rifai 1295-22). The control conferred is against the one or more of the following phytopathogens: Aspergillus flavus, Cercospora zeae-maydis, Fusarium monilforme, Fusarium graminearum, Helminthosporium maydis, Phoma lingam, Phomopsis helianthi, Phytopthera infestans, Pyricularia oryzae, Pythium ultimum, Rhizoctonia solani, Sclerotinia sclerotiorum, Ustilago maydis, and Verticillium dahliae. Further, this invention encompasses other homologous and heterologous sequences with a high degree of functional similarity.

The invention discloses cyclic dipeptide and application of the cyclic dipeptide in the preparation of a bactericide and belongs to the field of plant protection. The cyclic dipeptide is 3-isopropyl-pyrrolopiperazine-2,5-dione. Pathogen controlled by the bactericide is one or more than one of a group consisting of Botrytis cinerea, corynespora cassiicola, Ascochyta citrullina, Colletotrichum orbiculare, Pyricularia grisea, Candida albicans, Pellicularia, Fusarium oxysporum, Rhizoctonia solani, xanthomonas oryzae, aspergillus flavus and Fusarium moniliforme. The invention reports bactericidal activity of cyclo(L-proline-L-valine) for the first time and provides a new thinking for research and development of the bactericide. As a bactericide, the cyclic dipeptide cyclo(L-proline-L-valine) isderived from a secondary metabolite of Pseudomonas syringae, belongs to a natural product, is easy to degrade in the environment, and is not easy to cause harm o the environment.

The invention discloses a filamentous fungus promoter, a filamentous fungus terminator, and a plasmid containing the same. The filamentous fungus promoter has a base sequence represented by SEQ ID No.1, and the filamentous fungus terminator has a base sequence represented by SEQ ID No.2. The promoter of the invention is a strong promoter. The promoter has high-strength promotion effect in the hypha, spore and appresorium stages of pyricularia grisea. The promoter gene belongs to a gene of which the stability of the expression strength in cells of pyricularia grisea ranking in top 100. The gene is an important gene for removing active oxygen in cell and has a great significance for maintaining the normal operation of cells. The gene can be expressed in a large amount under the induction of active oxygen and is suitable for synthesizing a large amount of recombinant protein.

The invention belongs to the field of molecular biology and in particular relates to a molecular marker for detecting an anti-pyricularia grisea cav. gene in rice and special primers thereof. The molecular marker is a dominant molecular marker pi5-1-2 of the anti-pyricularia grisea cav. gene pi5. The molecular marker uses a primer pair SEQ ID NO:1 and SEQ ID NO:2 to amplify a nucleotide sequence from the total DNA (deoxyribonucleic acid) of rice, and can be applied to molecular marker-assisted selection of pi5 in rice pyricularia grisea cav. resistance breeding to improve the disease-resistant variety breeding efficiency and reduce the workload of field identification.

The invention discloses application of cyclic dipeptide cyclo-(S)-pro-(S)-phe to prevention and control of bacterial wilt and / or rice blast. The inventor finds out that the cyclic dipeptide cyclo-(S)-pro-(S)-phe has a very strong inhibition effect on rastonia solanacearum and provides a new option for preparation of a novel bacterial wilt biocontrol preparation for the first time; furthermore, the cyclic dipeptide cyclo-(S)-pro-(S)-phe also has a remarkable inhibition effect on spores of pyricularia grisea. A preparation method of the cyclic dipeptide cyclo-(S)-pro-(S)-phe, provided by the invention, takes escherichia coli GZ-34 as a strain, and comprises the steps of culturing, separating and purifying; the method comprises the simple steps and is simple to operate, so that industrial production is easy to carry out.

The invention discloses a 1,3,4-thiadiazole (oxadiazole)-phenazine-1-formamide compound. The structure of the compound is as shown in a formula (I), wherein R is at least one of hydrogen, halogen, nitryl, hydroxyl and alkyl or alkoxy of which the carbon atom sum is 1-4; and X is O or S. The 1,3,4-thiadiazole (oxadiazole)-phenazine-1-formamide compound disclosed by the invention is remarkably higher than shenqinmycin on the aspect of inhibiting the activity of fusarium graminearum, and the EC50 of a compound I-8 with the optimal activity is reduced by 95.29mu g . mL<-1> than that of PCA; and the compounds I-8 and I-22 are equivalent to shenqinmycin on the aspect of inhibiting the activities of rhizoctonia solani, Pyricularia grisea and phytophthora capsici. In addition, the preparation method provided by the invention is simple and rapid, high in yield and convenient for large-batch preparation in an actual production process.

The invention discloses a rapid immunoassay test strip for phytophthora infestans and application thereof. The test strip comprises a bottom plate, wherein a water absorption pad, a detection pad, a gold label pad and a sample pad are sequentially adhered to one surface of the bottom plate from top to bottom; every two adjacent pads are connected in an overlapped mode at the joint, a nitrocellulose membrane serves as a base pad of the detection pad, a quality control line and a detection line are transversely arranged on the nitrocellulose membrane from top to bottom, the detection line is located below the quality control line, the detection line is coated with an anti-pyricularia grisea polyclonal antibody 0550, and the quality control line is coated with goat anti-mouse IgG. The gold label pad is sprayed with a nanogold labeled anti-pyricularia oryzae monoclonal antibody. The specificity is high, and no cross reaction is caused to pythium aphanidermatum, pear rust water, pear rot, pear anthracnose and the like of Asian pears; the sensitivity of the test strip can reach 1*10<5> cfu / mL, and the test strip can be used for rapid screening of pythium aphanidermatum in fields.

The invention discloses a fungicide composition of fluoride containing ether bacteria amide and validamycin, which consists of active components and supplementary materials. The active components are fluoride ether bacteria amide and validamycin, the weight ratio of fluoride ether bacteria amide to validamycin is 1:4-4:1, and the total content of the active components is 10%-60%. The fungicide composition provided by the invention is specially utilized to prevent and control rice sheath blight disease, pyricularia grisea, rice false smut, wheat sharp eyespot, vegetable leaf spot disease, vegetable powdery mildew, pear scab, coniothyrium diplodiella, apple anthracnose, macrophoma kawatsukai, cucumber corynespora target leaf spot and strawberry fusarium wilt and the like.

The invention discloses a hydrocarbon-oxygen imino group dibenzo caprolactam derivative CAUWL-2010, as well as a preparation method an application of the derivative. The constitutional formula of the derivative CAUWL-2010 is shown as in formula I, wherein R1 is H, C1, Br or I, R2 is C1-C4 alkyl, allyl, n-heptyl, myristyl, hexadecyl, benzyl or benzyl with 1-2 substituents; and the substituents in the benzyl with 1-2 substituents are F, C1, CH3, CF3 or NO2. The compound CAUWL-2010 is obtained by the reaction of the compound shown in the formula II with hydrocarbon-oxygen amine hydrochloride. Through bactericidal activity, the result indicates that both the compound CAUWL-2010 and the preparation of the compound CAUWL-2010 have excellent restraining effect for the growth of sclerotinia scleotiorum, cucumber Botrytis Cinerea, asparagus phomopsis asparagi and pyricularia grisea. (formula I) (formula II).

The invention discloses a method for lowering sensitivity of a pyricularia grisea effect protein gene overexpression strain to pH. The method comprises the following steps: treating pyricularia grisea effect protein gene overexpression strain spores with pH5 and pH8; performing statistics to obtain spore yield, sprouting rate and appressorium forming rate. Compared with the prior art, the method has the advantages that the sensitivity of the pyricularia grisea effect protein gene overexpression strain to different pH can be easily, conveniently and accurately determined, and an important reference basis is laid for rapid comprehension of the pathogenicity of a pathogenic bacteria effect protein gene overexpression strain.

The invention discloses a bacillus siamensis BsNlG13 strain for inhibiting pyricularia grisea and promoting seed germination and application thereof. The bacillus siamensis BsNlG13 strain is preserved in Guangdong Microbial Culture Collection Center on December 25, 2020, and the preservation number of the bacillus siamensis BsNlG13 strain is GDMCC NO: 61391. The research shows that bacillus siamensis BsNlG13 strain fermentation liquor can effectively inhibit the growth of the pyricularia grisea, so that hyphae of the pyricularia grisea deform, and the normal growth of the pyricularia grisea cannot be realized; and meanwhile, the bacillus siamensis BsNlG13 strain fermentation broth has a significant promotion effect on rice seed germination. The bacillus siamensis BsNlG13 strain disclosed by the invention can be used as a good biocontrol and plant production regulation resource, is used for preventing and controlling the pyricularia grisea and promoting rice seed germination, and has a good application prospect.

The invention belongs to the technical field of screening and application of functional microorganisms, and particularly relates to bacillus subtilis CS30 and application thereof. The bacillus subtilis CS30 capable of producing lipopeptide is collected in China general microbiological culture collection center (address: 3#, Yard 1, Beichen West Road, Chaoyang District, Beijing (Institute of Microbiology, Chinese Academy of Sciences)) on October 31, 2018 with a collection number of CGMCC NO.16666. The bacillus subtilis CS30 of the invention is separated in deep sea cold spring sediment, and hasthe characteristic of restraining the growth of plant pathogenic fungi pyricularia grisea and tumor cells.

The invention discloses a pyricularia grisea spore production culture medium and a preparation method thereof. The culture medium comprises raw tartary buckwheat / wheat bran, agar and water. The preparation method of the culture medium comprises the following steps that the raw tartary buckwheat / wheat bran is mixed with water to be boiled, filtering is carried out, the agar is added into a filter liquor, after heating and melting are carried out, water is added until constant volume, and sterilization is carried out for standby application at high temperature and high pressure; before inoculation, the culture medium is heated and melted, and then cooled, and the culture medium is poured into a culture dish before solidification. The pyricularia grisea spore production culture medium has theadvantages that the formula components are easy to obtain, the formula is simple and reasonable, the preparation method of the culture medium is simple and convenient and easy to operate, the cultureprocess is not easy to contaminate, and the time and labor are saved.