66 results about "Biological clock" patented technology

The invention relates to the technical field of biological prevention and control, in particular to a method for analyzing rice leaf rhythm change after rice is infected by pyricularia grisea and application of the method in accurate prevention and control of rice blast. The analysis method comprises the following steps: (1) spraying a pyricularia grisea spore suspension on rice leaves; (2) carrying out whole-genome transcriptome analysis on the rice leaves, and carrying out clustering heat map; (3) analyzing expression conditions of the biological clock genes and the phytochrome related genes in the circadian rhythm pathway of the rice leaves, and analyzing the biological clock genes and the phytochrome related genes by a MetaCycle method; and (4) analyzing the deposition amount of H2O2 and callose in the rice leaves. Determining the dynamic change rule of the rice immune process caused by magnaporthe oryzae infection according to the method; in combination with the characteristics of small molecules, small-molecule pesticides are reasonably applied at a specific time point aiming at the rice blast, so that efficient and accurate prevention and control of the rice blast are realized.

The invention relates to the technical field of medical typing decision, in particular to a myocardial infarction metabolic rhythm intelligent typing and administration decision method and system.The myocardial infarction metabolic rhythm intelligent typing and administration decision method comprises the steps that biological clock genes BMAL1 mRNA, a USP2-BCAT2 compound and branched chain amino acid BCAs are synchronously detected through a multi-index joint detection kit; a BCAs circadian rhythm curve is collected through a wearable monitoring patch integrating a sweat BCAs sensor and an electrocardiogram monitoring module; the invention relates to a random forest model myocardial infarction metabolic rhythm typing intelligent model. A multi-dimensional dynamic typing system is established by integrating BMAL1 methylation, a USP2-BCAT2 compound and BCAs rhythm fluctuation, and high-risk subtypes needing metabolic intervention are accurately distinguished; the concentration fluctuation of the BCAs is monitored in real time through the intelligent patch, and the abnormal metabolism state is pre-warned; and the administration opportunity is automatically optimized based on the individualized biological clock phase, and clinical pain points such as insufficient typing precision, abnormal early warning lag and administration time sequence mismatch are solved.

A method and device for predicting the bull semen quality. The method comprises: on the basis of the variation of methylation level values, using known methylation spectrum data of methylation sites of a bull sperm to select 83 methylation sites; on the basis of an elastic net regression algorithm, using the selected methylation sites to construct an epigenetic clock; and using the epigenetic clock to predict the age of a bull to be detected. The age of a bull can be predicted on the basis of an epigenetic clock, and the bull semen quality can be predicted on the basis of an epigenetic acceleration. The present invention can be applied to bull variety breeding and bull breeding.

The invention discloses an application of a 5, 7-dihydroxy flavonoid compound in preparation of a circadian rhythm regulation product. It is found for the first time that 5, 7-dihydroxy flavonoid compounds can enable cell phases to move backwards, regulate expression of core biological clock protein, inhibit cell HDAC3 activity, reduce REV-ERB alpha protein expression stability and restore mouse pressure to aggravate biological clock protein expression disorder after colitis, and therefore the effect of preventing and treating colitis is achieved. Therefore, the 5, 7-dihydroxy flavonoid compound can be used as an anti-colitis drug targeting a biological clock system, and has important significance in treating related diseases caused by biological clock disorder.

Disclosed are a method for predicting semen quality of a bull, a device therefor, and a computer-readable storage medium. The method screens 83 CpG sites from known methylation profile data of bull sperm based on variability in methylation levels, and constructs an epigenetic clock using elastic net regression with the screened CpG sites. The epigenetic clock is used to predict an age of a subject bull. Semen quality is predicted based on epigenetic age acceleration, defined as a difference between the predicted epigenetic age and a chronological age of the bull, and may be used for selection and breeding of bulls.

The invention relates to the field of economic animal feeding, and particularly discloses a technical method for promoting efficient and healthy breeding of bumblebees based on circadian rhythm, which comprises the following steps: breaking the diapause state of queen bees by using high-concentration COs, recovering the activity of the queen bees, transferring the recovered queen bees into an early-stage queen bee nesting feeding box, and providing normal pollen and liquid food; putting the queen bee breeding box into an incubator, simulating a natural photoperiod environment at the temperature of 28-29 DEG C and the humidity of 50-60%, and helping the queen bee to adapt to the circadian rhythm; at the beginning, the feeding amount of the queen bees every day is equal to the size of soybeans, and after the first batch of worker bees are hatched, the queen bees, the worker bees and honeycombs are transferred into a larger feeding box; according to the method, the biological clock of the queen bee is adjusted by controlling time service factors such as illumination, temperature, humidity and food, the survival rate of the queen bee is remarkably increased, the growth and development of the queen bee and the health state of a swarm are improved, and the technical method for promoting efficient and healthy breeding of the bumblebees based on the circadian rhythm is established.

A live body stimulation system comprising: a body temperature measurement unit (101) for measuring the body temperature of a user; a calculation unit (102) for extracting, from the body temperature of the user, a feature amount generated by the biological clock of the user; a stimulus output unit (104) for applying a stimulus to the body of the user; and a control unit (103) for controlling, on the basis of the feature amount, the stimulus applied to the body of the user by the stimulus output unit (104).

The present invention relates to an in vitro method for predicting the chronological age of a test Chinese Hamster Ovary (CHO) cell or population of test CHO cells, the method comprising the steps of: (a) determining the test methylation level of a set of specific CpG sites from genomic material obtained from the test CHO cell or from the population of test CHO cells from a CHO cell line using a DNA-methylation bead-based array; (b) comparing the test methylation level determined from step (a) with a reference methylation level of the same set of specific CpG sites from an age-correlated reference sample, thereby establishing the epigenetic age and predicting the chronological age of the test CHO cell or the population of test CHO cells wherein the set of specific CpG sites comprises at least 10%, preferably 30%, more preferably 50% of the CpG sites indicated in Table 1.

A method of determining and presenting a protocol for adjusting a circadian rhythm of a user including receiving first and second indications of first and second intended circadian shifts comprising shift magnitudes, receiving first and second indications of first and second user circadian phases, defining first and second user circadian shift protocols responsive to the shift magnitude and the indication of the user circadian phase, the user circadian shift protocol comprising one or more activities scheduled to be performed at a certain date and time, the activities comprising at least one of a light exposure or light avoidance activity, a chronobiotic activity, a nutritional consumption activity, a physical activity, and a rest activity. The method may further provide indications to perform each activity on first and second user devices.

The invention discloses a biological clock protein related to a soybean flowering period and a soybean maturation period as well as a coding gene and application of the biological clock protein. Statistical analysis on the flowering period and the mature period of the soybean homozygous mutant plant with the Gmrve4d gene knocked out shows that compared with wild soybeans, the flowering period and the mature period of the soybean homozygous mutant plant with the Gmrve4d gene knocked out are remarkably shortened under the conditions of long sunlight, short sunlight and natural light. The invention discovers that the Gmrve4d protein can regulate and control the flowering period and the mature period of the plant for the first time, and has important significance on plant variety adaptability improvement and breeding of new plant varieties capable of adapting to specific regions by using a molecular breeding means.

The application discloses a molecular marker of a wheat biological clock gene TaRVE7-A1 and application thereof, and belongs to the technical field of crop selection and cultivation. The molecular marker is a KASP marker, including RVE7-A1-SNP-709269692 and RVE7-A1-SNP-709269783, the former is obtained by amplification of a primer group shown in SEQ ID NO:9, SEQ ID NO:10 and SEQ ID NO:11, two forward primers are marked with FAM and HEX at the 5' end respectively, and the latter is obtained by amplification of a primer group shown in SEQ ID NO:12, SEQ ID NO:13 and SEQ ID NO:14, two forward primers are marked with FAM and HEX at the 5' end respectively. By applying the molecular marker developed in the application, excellent haplotypes can be identified in wheat line selection, cost is saved, and selection efficiency is greatly improved.

Disclosed herein are compositions and methods useful for reducing biological age measured by DNA methylation and the treatment or amelioration of various diseases, disorders, or conditions promoted by increased biological age. Some aspects pertain to a pharmaceutical composition comprising 17-ethynyl-10R, 13S-dimethyl 2, 3, 4, 7, 8R, 9S, 10, 11, 12, 13, 14S, 15, 16, 17-hexadecahydro-1H-cyclopenta[a]phenanthrene-3R, 7R, 17S-triol, including solid states thereof. Also presented herein is the surprising discovery that exposing a subject to the compositions disclosed herein can treat, reduce, or ameliorate a condition related to biological clocks.

Provided herein are methods and compositions for an epigenetic clock comprising differentiation-independent methylation sites to asses biological age of a human subject.

The invention discloses an intelligent sleep-aiding control system and method integrating phototherapy and biological rhythm, and the system comprises a biological rhythm monitoring device which is used for collecting the biological rhythm data of a user, and transmitting the data to a computing terminal through a Bluetooth module; the computing terminal is used for analyzing the biological rhythm data through a sleep staging algorithm, judging the current sleep stage of the user, generating a corresponding control instruction and transmitting the control instruction to the intelligent sleep-aiding phototherapy pillow through the Bluetooth module; the intelligent sleep-aiding phototherapy pillow is used for adjusting the phototherapy function in real time according to the received control instruction so as to adapt to the current sleep stage of the user, and therefore automatic control and real-time adjustment of the sleep process of the user are achieved. By accurately simulating the change of natural light, adjusting the biological clock of the user, optimizing the sleep-aiding effect, improving the intelligent level, simplifying the system structure and reducing the cost, the requirements for an efficient and intelligent sleep-aiding system in modern life are met.

The invention discloses a stem cell culture generation identification method based on an epigenetics clock. Belongs to the field of biotechnology and molecular biology, and designs a specific multiple PCR primer pool and establishes a high-precision generation prediction model by screening eight CpG sites highly related to stem cell replicative senescence. The method comprises the following steps: firstly, carrying out hydrosulfite conversion on sample DNA (Deoxyribonucleic Acid), carrying out targeted amplification by adopting a primer group with an optimized ratio, calculating a methylation level through high-throughput sequencing, and substituting into a formula to output a result. The model has small mean absolute error and is significantly related to aging function indexes. Compared with traditional chip detection, the cost is reduced, the detection period is shortened, and the method is suitable for release inspection of stem cell treatment products, anti-aging drug screening and cell bank quality management.

The invention discloses a diabetes nutrition intervention system based on a four-dimensional theory, which belongs to the field of nutrition intervention and comprises a precise nutrition intervention module, a metabolic rhythm regulation module, an intestinal flora optimization module, an emotional and lifestyle management module, a data acquisition and analysis module and a scheme regulation module. Through the synergistic effect of four dimensions of precise nutrition intervention, metabolic rhythm regulation, intestinal flora optimization and emotional and lifestyle management, the limitation of a single intervention mode is broken through, and systematic regulation of blood sugar is realized from multiple aspects of nutrition metabolism, biological clock regulation, intestinal microecological balance, physical and mental states and the like; compared with traditional single diet or exercise intervention, the insulin sensitivity can be more remarkably improved, and the blood sugar fluctuation range is reduced; according to the invention, food intervention is taken as a core, and life style adjustment is combined, so that the side effects of gastrointestinal reaction, liver and kidney function burden and the like possibly caused by long-term drug treatment are avoided.

A system and method evaluate the efficiency of circadian-effective luminaires. Such circadian-effective luminaires affect the master biological clock in the brain, which regulates the daily timings of behavioral activities and physiological functions. Disruption of circadian rhythms can lead to poor performance and poor health, while consistent exposure to bright days and dim nights is necessary for circadian entrainment, and thus for good sleep and good health. The system and method provide a standard test for assessing the efficiency of luminaires for providing circadian-effective light to building occupants. The system and method measure and quantify luminaire efficiency based on the electrical watts needed, termed SOWatt, to reach a circadian stimulus (CS) criterion of 0.3 at the eyes of a standard observer (SO). The system and method can be applied to ceiling mounted, accent, and table-top luminaires.

The present invention relates to an application of a thioamide compound in regulating circadian rhythm. The thioamide compound of the present invention has been subjected to in vitro and in vivo experiments, showing obvious rapid regulation of circadian rhythm disorders, reduction of synchronization time of circadian rhythm phase shifts, and regulation of the expression of hypothalamic circadian clock proteins Per2, Bmal1 and related phosphorylated proteins P‑Per2, P‑CaMKII. Therefore, it can be used to treat jet lag, shift work, continuous multi-day and night work or circadian rhythm-related sleep disorders, and has the characteristics of high efficiency and low toxicity, can improve and guarantee work ability, and is of great significance for people with circadian rhythm disorders who work in night shifts, shift work, and long-distance cross-time zone flights.

The invention relates to an anti-aging composition capable of regulating circadian rhythm and retarding social contact time difference, a cosmetic and a preparation method of the anti-aging composition. The anti-aging composition is prepared from hydrolyzed yeast protein wrappage, rice fermentation liquor and L-sodium lactate in a mass ratio of (0.1-5): (0.1-10): (0.1-2). Compared with the prior art, by adjusting internal mechanisms such as biological clock and by simulating or enhancing natural circadian rhythm of the skin, physiological functions of the skin are optimized, so that skin aging is delayed. The skin can be repaired and regenerated at correct time, so that a more lasting anti-aging effect is realized.

The invention provides an epigenetic clock calculation model and a construction method, and belongs to the technical field of bioinformatics. A mathematical relationship between DNA methylation and age (month age) is established by taking northeast tiger as an example and taking excrement of the northeast tiger as a material, a method for constructing a calculation model agent of an epigenetic clock is provided, and the problem that in the prior art, materials needed for calculating the age of large animals (including wild animals) are not easy to obtain is solved. The calculation model of the epigenetic clocks is applied to calculation of the age of large animals or wild animals, selected materials are materials which are free of excrement damage and easy to obtain, and the accuracy of age calculation is higher than that of a calculation method using blood in a traditional method.

The invention relates to the technical field of light environment detection, and discloses a natural light exposure dynamic evaluation method and system, a wearable device and a storage medium, and the method comprises the steps: carrying out the scene analysis of a light source which is judged to be natural light, precisely classifying an exposure event into direct exposure, indirect exposure or invalid exposure, and only accumulating effective exposure duration. The system generates a specifically executable contextualized health intervention suggestion based on the difference between the exposure duration and the health goals. The problems of misjudgment of light source types, lack of scene perception, disjunction of intervention suggestions and user states and the like are solved, the natural light monitoring precision and the health intervention compliance are improved, and the method can be applied to the health management fields of biological clock adjustment, myopia prevention and control and the like.

The invention relates to the technical field of digital psychotherapy, and discloses a digital meta-cognitive insomnia treatment and intervention method, which comprises the following steps of: receiving initial scale data, calculating a physical interactive wake-up baseline threshold value and initializing a main control state machine; obtaining user interaction data, executing bottom layer isolation packaging, and distributing an initial locking time parameter; collecting multi-dimensional terminal behavior characteristics, calculating a monitoring wake-up parameter, and when the parameter is greater than a threshold value, prolonging a locking parameter and accumulating a state machine rollback mark; outputting a cross-modal physical stimulation control signal according to the mark; and executing one-way sliding rendering on the unlocking data when the countdown returns to zero, and performing a circulation stage when the variable quantity returns to zero. According to the method, data asynchronous isolation is executed by bypassing semantic analysis, and physical stimulation and locking parameters are dynamically output in combination with physical behavior characteristics, so that abnormal cognitive rumination migration is converted into bottom hardware redirection intervention, sleep wake-up overshoot fluctuation can be suppressed, and the regulation recovery time of a biological clock steady state is shortened.

The invention belongs to the field of gene engineering, and relates to application and a method of a wheat biological clock gene TaPRR95 in regulating and controlling a recent rhythm cycle of wheat. Rhythmicity change of the growth rate of a second leaf of a wheat seedling is taken as a quantitative index, and recent rhythm cycles of wild wheat Fielder and TaPRR95 mutants are compared, so that the fact that the recent rhythm cycles of wheat are remarkably shortened due to function deletion of the TaPRR95 gene is found. Meanwhile, the recent rhythm cycle quantitative detection method based on the growth rate of the second leaf of the wheat seedling is established, and the method is easy and convenient to operate, rapid in detection and high in quantitative precision. The key function of the TaPRR95 gene in wheat recent rhythm regulation is defined, the blank of wheat PRR family gene rhythm regulation function research is filled, important gene resources and technical support are provided for wheat recent rhythm regulation mechanism analysis and molecular breeding, and important theoretical significance and application prospects are achieved.

The invention discloses a biological clock disorder monitoring device and monitoring method, and relates to the technical field of biological clock monitoring, and the biological clock disorder monitoring device comprises a multi-dimensional biological clock associated data acquisition unit, a biological clock data analysis unit and a biological clock disorder display unit. According to the invention, the physiological parameter acquisition device, the work and rest parameter self-reporting terminal and the diet parameter self-reporting terminal are respectively used for acquiring physiological parameter data, work and rest time parameters and dining time parameters of a monitored target, and data acquisition does not cause trouble to privacy of the monitored target; the biological clock data analysis unit can calculate a biological clock disorder degree score according to a biological clock disorder degree algorithm, and finally, the biological clock disorder display unit judges and displays the target biological clock disorder degree based on a threshold value, so that the biological clock disorder degree of a monitored target can be quantified, and the biological clock disorder degree is judged according to a quantization result; and the monitoring result is more accurate.

The invention relates to the technical field of health food preparation, and particularly discloses an oyster peptide composition capable of improving sleep and a preparation method thereof, the oyster peptide composition is prepared from 50-60 parts of oyster peptide powder, 10-15 parts of gamma-aminobutyric acid GABA, 15-20 parts of spina date seed extract, 0.3-0.5 part of melatonin, 5-8 parts of vitamin B6, 10-15 parts of lycium barbarum polysaccharide and 8-10 parts of functional probiotic powder; the finished product oyster peptide composition prepared by the preparation method disclosed by the invention is high in safety of natural components, and the oyster peptide is rich in micromolecule active peptide, taurine, zinc, selenium and other microelements and has multiple sleep aiding mechanisms; neurotransmitters can be adjusted, and active peptides can promote GABA secretion, relieve anxiety and enhance the sedation effect; the traditional Chinese medicine composition can improve the biological clock, improve the serum melatonin level, help to adjust the circadian rhythm, promote deep sleep, adjust the function of a central nervous system and increase the deep sleep proportion, and has the functions of resisting oxidation and relieving fatigue.

The invention belongs to the technical field of medicines, and particularly relates to application and application of pemetrexed in prevention and treatment of biological clock disorder. Pemetrexed can be directly combined with biological clock protein Bmal1, the amplitude of cell biological rhythm is remarkably inhibited, time difference of mice can be adjusted more quickly, biological clock disorder is effectively relieved, and pemetrexed has a certain treatment effect on biological clock disorder.

The invention relates to the technical field of biological medicine, and discloses a therapeutic target for regulating circadian rhythm disorder, and the therapeutic target is BMAL1-USP2-BCAT2; the therapeutic target can be used for adjusting the therapeutic target of the circadian rhythm disorder in preparation of products for relieving or detecting circadian rhythm disorder related injuries, participates in treatment of myocarditis, cardiovascular diseases and Alzheimer's disease related to the circadian rhythm disorder, and provides a circadian rhythm disorder related myocardial protective agent. The composition comprises an active component and a delivery system for delivering the active component, and the active component is used for targeted regulation of a therapeutic target BMAL1-USP2-BCAT2. According to the invention, three-dimensional intervention of biological clock-ubiquitination-metabolism is realized through a BMAL1-USP2-BCAT2 time sequence regulation and control network, the limitation of existing single-target treatment is broken through, and a brand-new target is provided for regulating treatment of diseases related to circadian rhythm disorder.

The present disclosure relates to use of DNA methylation states of retroelements as an epigenetic biomarker of aging. Disclosed herein are retroelement-based epigenetic clocks, and methods for determining age (epigenetic, biological or chronological) of a subject by measuring DNA methylation status at CpG loci located in at least one retroelement. Also, disclosed are methods of treatment, prevention, assessing impact or efficacy, and prediction of outcome based on epigenetic age determinations made in accordance with the described methods and using retroelement-based epigenetic clocks. Also disclosed is use of an antiretroviral therapy for treatment of an age-associated disease or condition, rejuvenation and / or reducing epigenetic age.

The disclosure relates to methods for disrupting the function of upstream open reading frames (uORFs) in the 5' untranslated regions (UTRs) of plant clock regulator genes by introducing or selecting mutations, for example, using a gene editing construct, such as CRISPR / Cas (including Cas9, Cas12, MAD7, or SHARC™), which contains a polynucleotide encoding a Cas enzyme and at least one guide RNA targeting a uORF sequence in a plant. These guide RNAs specifically target uORFs in 5'UTRs associated with main open reading frames (ORFs) encoding polypeptides that are homologous to, or share at least 30%–100% identity with sequences set forth specified SEQ ID Nos disclosed therein. The disclosure also covers polypeptides (uPEPs) encoded by uORFs within sequences set forth in additional specified SEQ ID NOs or their homologous nucleotide sequences.

The invention relates to the technical field of bioengineering, in particular to a coccinella septempunctata biological clock Timeless (Cs-Tims) gene as well as an inhibitor and application thereof. The invention provides a nucleotide sequence of a coccinella septempunctata biological clock Cs-Tima gene as shown in SEQ ID NO. 1 and an amino acid sequence as shown in SEQ ID NO. 2; the invention also provides an application of the biological clock Cs-Tims gene inhibitor in relieving biological diapause. After the biological clock Cs-Timm gene provided by the invention is subjected to dsRNA inhibition expression, the diapause of the coccinella septempunctata can be relieved, the dsRNA of the Cs-Timm gene can be used as a related preparation and product for relieving the diapause of the coccinella septempunctata, the development of the natural enemy industry is promoted, and the application prospect is wide.