30 results about "Biological clock" patented technology

The invention relates to the technical field of biological prevention and control, in particular to a method for analyzing rice leaf rhythm change after rice is infected by pyricularia grisea and application of the method in accurate prevention and control of rice blast. The analysis method comprises the following steps: (1) spraying a pyricularia grisea spore suspension on rice leaves; (2) carrying out whole-genome transcriptome analysis on the rice leaves, and carrying out clustering heat map; (3) analyzing expression conditions of the biological clock genes and the phytochrome related genes in the circadian rhythm pathway of the rice leaves, and analyzing the biological clock genes and the phytochrome related genes by a MetaCycle method; and (4) analyzing the deposition amount of H2O2 and callose in the rice leaves. Determining the dynamic change rule of the rice immune process caused by magnaporthe oryzae infection according to the method; in combination with the characteristics of small molecules, small-molecule pesticides are reasonably applied at a specific time point aiming at the rice blast, so that efficient and accurate prevention and control of the rice blast are realized.

The invention discloses an application of a 5, 7-dihydroxy flavonoid compound in preparation of a circadian rhythm regulation product. It is found for the first time that 5, 7-dihydroxy flavonoid compounds can enable cell phases to move backwards, regulate expression of core biological clock protein, inhibit cell HDAC3 activity, reduce REV-ERB alpha protein expression stability and restore mouse pressure to aggravate biological clock protein expression disorder after colitis, and therefore the effect of preventing and treating colitis is achieved. Therefore, the 5, 7-dihydroxy flavonoid compound can be used as an anti-colitis drug targeting a biological clock system, and has important significance in treating related diseases caused by biological clock disorder.

The invention relates to the field of economic animal feeding, and particularly discloses a technical method for promoting efficient and healthy breeding of bumblebees based on circadian rhythm, which comprises the following steps: breaking the diapause state of queen bees by using high-concentration COs, recovering the activity of the queen bees, transferring the recovered queen bees into an early-stage queen bee nesting feeding box, and providing normal pollen and liquid food; putting the queen bee breeding box into an incubator, simulating a natural photoperiod environment at the temperature of 28-29 DEG C and the humidity of 50-60%, and helping the queen bee to adapt to the circadian rhythm; at the beginning, the feeding amount of the queen bees every day is equal to the size of soybeans, and after the first batch of worker bees are hatched, the queen bees, the worker bees and honeycombs are transferred into a larger feeding box; according to the method, the biological clock of the queen bee is adjusted by controlling time service factors such as illumination, temperature, humidity and food, the survival rate of the queen bee is remarkably increased, the growth and development of the queen bee and the health state of a swarm are improved, and the technical method for promoting efficient and healthy breeding of the bumblebees based on the circadian rhythm is established.

A live body stimulation system comprising: a body temperature measurement unit (101) for measuring the body temperature of a user; a calculation unit (102) for extracting, from the body temperature of the user, a feature amount generated by the biological clock of the user; a stimulus output unit (104) for applying a stimulus to the body of the user; and a control unit (103) for controlling, on the basis of the feature amount, the stimulus applied to the body of the user by the stimulus output unit (104).

The present invention relates to an in vitro method for predicting the chronological age of a test Chinese Hamster Ovary (CHO) cell or population of test CHO cells, the method comprising the steps of: (a) determining the test methylation level of a set of specific CpG sites from genomic material obtained from the test CHO cell or from the population of test CHO cells from a CHO cell line using a DNA-methylation bead-based array; (b) comparing the test methylation level determined from step (a) with a reference methylation level of the same set of specific CpG sites from an age-correlated reference sample, thereby establishing the epigenetic age and predicting the chronological age of the test CHO cell or the population of test CHO cells wherein the set of specific CpG sites comprises at least 10%, preferably 30%, more preferably 50% of the CpG sites indicated in Table 1.

The invention discloses a biological clock protein related to a soybean flowering period and a soybean maturation period as well as a coding gene and application of the biological clock protein. Statistical analysis on the flowering period and the mature period of the soybean homozygous mutant plant with the Gmrve4d gene knocked out shows that compared with wild soybeans, the flowering period and the mature period of the soybean homozygous mutant plant with the Gmrve4d gene knocked out are remarkably shortened under the conditions of long sunlight, short sunlight and natural light. The invention discovers that the Gmrve4d protein can regulate and control the flowering period and the mature period of the plant for the first time, and has important significance on plant variety adaptability improvement and breeding of new plant varieties capable of adapting to specific regions by using a molecular breeding means.

The application discloses a molecular marker of a wheat biological clock gene TaRVE7-A1 and application thereof, and belongs to the technical field of crop selection and cultivation. The molecular marker is a KASP marker, including RVE7-A1-SNP-709269692 and RVE7-A1-SNP-709269783, the former is obtained by amplification of a primer group shown in SEQ ID NO:9, SEQ ID NO:10 and SEQ ID NO:11, two forward primers are marked with FAM and HEX at the 5' end respectively, and the latter is obtained by amplification of a primer group shown in SEQ ID NO:12, SEQ ID NO:13 and SEQ ID NO:14, two forward primers are marked with FAM and HEX at the 5' end respectively. By applying the molecular marker developed in the application, excellent haplotypes can be identified in wheat line selection, cost is saved, and selection efficiency is greatly improved.

Provided herein are methods and compositions for an epigenetic clock comprising differentiation-independent methylation sites to asses biological age of a human subject.

The invention discloses a stem cell culture generation identification method based on an epigenetics clock. Belongs to the field of biotechnology and molecular biology, and designs a specific multiple PCR primer pool and establishes a high-precision generation prediction model by screening eight CpG sites highly related to stem cell replicative senescence. The method comprises the following steps: firstly, carrying out hydrosulfite conversion on sample DNA (Deoxyribonucleic Acid), carrying out targeted amplification by adopting a primer group with an optimized ratio, calculating a methylation level through high-throughput sequencing, and substituting into a formula to output a result. The model has small mean absolute error and is significantly related to aging function indexes. Compared with traditional chip detection, the cost is reduced, the detection period is shortened, and the method is suitable for release inspection of stem cell treatment products, anti-aging drug screening and cell bank quality management.

The invention relates to the technical field of light environment detection, and discloses a natural light exposure dynamic evaluation method and system, a wearable device and a storage medium, and the method comprises the steps: carrying out the scene analysis of a light source which is judged to be natural light, precisely classifying an exposure event into direct exposure, indirect exposure or invalid exposure, and only accumulating effective exposure duration. The system generates a specifically executable contextualized health intervention suggestion based on the difference between the exposure duration and the health goals. The problems of misjudgment of light source types, lack of scene perception, disjunction of intervention suggestions and user states and the like are solved, the natural light monitoring precision and the health intervention compliance are improved, and the method can be applied to the health management fields of biological clock adjustment, myopia prevention and control and the like.

The invention relates to the technical field of digital psychotherapy, and discloses a digital meta-cognitive insomnia treatment and intervention method, which comprises the following steps of: receiving initial scale data, calculating a physical interactive wake-up baseline threshold value and initializing a main control state machine; obtaining user interaction data, executing bottom layer isolation packaging, and distributing an initial locking time parameter; collecting multi-dimensional terminal behavior characteristics, calculating a monitoring wake-up parameter, and when the parameter is greater than a threshold value, prolonging a locking parameter and accumulating a state machine rollback mark; outputting a cross-modal physical stimulation control signal according to the mark; and executing one-way sliding rendering on the unlocking data when the countdown returns to zero, and performing a circulation stage when the variable quantity returns to zero. According to the method, data asynchronous isolation is executed by bypassing semantic analysis, and physical stimulation and locking parameters are dynamically output in combination with physical behavior characteristics, so that abnormal cognitive rumination migration is converted into bottom hardware redirection intervention, sleep wake-up overshoot fluctuation can be suppressed, and the regulation recovery time of a biological clock steady state is shortened.

The invention belongs to the field of gene engineering, and relates to application and a method of a wheat biological clock gene TaPRR95 in regulating and controlling a recent rhythm cycle of wheat. Rhythmicity change of the growth rate of a second leaf of a wheat seedling is taken as a quantitative index, and recent rhythm cycles of wild wheat Fielder and TaPRR95 mutants are compared, so that the fact that the recent rhythm cycles of wheat are remarkably shortened due to function deletion of the TaPRR95 gene is found. Meanwhile, the recent rhythm cycle quantitative detection method based on the growth rate of the second leaf of the wheat seedling is established, and the method is easy and convenient to operate, rapid in detection and high in quantitative precision. The key function of the TaPRR95 gene in wheat recent rhythm regulation is defined, the blank of wheat PRR family gene rhythm regulation function research is filled, important gene resources and technical support are provided for wheat recent rhythm regulation mechanism analysis and molecular breeding, and important theoretical significance and application prospects are achieved.

The invention relates to the technical field of biological medicine, and discloses a therapeutic target for regulating circadian rhythm disorder, and the therapeutic target is BMAL1-USP2-BCAT2; the therapeutic target can be used for adjusting the therapeutic target of the circadian rhythm disorder in preparation of products for relieving or detecting circadian rhythm disorder related injuries, participates in treatment of myocarditis, cardiovascular diseases and Alzheimer's disease related to the circadian rhythm disorder, and provides a circadian rhythm disorder related myocardial protective agent. The composition comprises an active component and a delivery system for delivering the active component, and the active component is used for targeted regulation of a therapeutic target BMAL1-USP2-BCAT2. According to the invention, three-dimensional intervention of biological clock-ubiquitination-metabolism is realized through a BMAL1-USP2-BCAT2 time sequence regulation and control network, the limitation of existing single-target treatment is broken through, and a brand-new target is provided for regulating treatment of diseases related to circadian rhythm disorder.

The disclosure relates to methods for disrupting the function of upstream open reading frames (uORFs) in the 5' untranslated regions (UTRs) of plant clock regulator genes by introducing or selecting mutations, for example, using a gene editing construct, such as CRISPR / Cas (including Cas9, Cas12, MAD7, or SHARC™), which contains a polynucleotide encoding a Cas enzyme and at least one guide RNA targeting a uORF sequence in a plant. These guide RNAs specifically target uORFs in 5'UTRs associated with main open reading frames (ORFs) encoding polypeptides that are homologous to, or share at least 30%–100% identity with sequences set forth specified SEQ ID Nos disclosed therein. The disclosure also covers polypeptides (uPEPs) encoded by uORFs within sequences set forth in additional specified SEQ ID NOs or their homologous nucleotide sequences.

This invention provides an artificial lighting system and method for promoting circadian health. [Solution] Methods, systems, and computer-readable media utilizing artificial lighting regulate the human circadian clock. By utilizing light-emitting diodes (LEDs) that emit wavelengths associated with the human opsin absorption spectrum, the device can stimulate human opsins. For example, the device replicates normal sunlight (for example, based on the season or time of day) by emitting light that changes in rhythmic intensity or spectral modulation.

A combination of medications and medication doses is disclosed whereby age-related changes in systemic inflammation, cancer risk, heart disease risk, CD38 expression, hair color, thymotrophic hormones, immune cell populations, the CD4 / CD8 cell ratio, bone marrow density, thymus structure, kidney function, and epigenetic age can be reversed in humans. Surprisingly, agents that accelerate the growth of cells reduce cancer risk, agents that intensify immune responses attenuate age-related inflammation, agents with no prior connection to hair color reverse age-related hair whitening, and a combination of agents that induces IGF-1, a hormone previously thought to drive systemic aging, results in a reversal of systemic aging as documented by an epigenetic clock. Medication combinations useful in the present invention include human growth hormone (GH) or GH releasers, dehydroepiandrosterone (DHEA), and metformin.

The invention discloses a GIS (Geographic Information System)-based rice low-temperature cold injury risk assessment method and system. The method comprises the following steps of 1, constructing rice physiological behavior time sequence data; 2, constructing a day and night behavior phase model; 3, mapping the rice physiological behavior time sequence data to a day and night behavior phase model, setting a biological clock drift judgment condition, and generating a biological clock drift signal when the condition is met; 4, constructing a fusion feature vector sequence; 5, inputting the fusion feature vector sequence into an improved TimesNet model, embedding a double-time-domain gating residual filtering mechanism, and outputting a rhythm anomaly feature vector; step 6, constructing a cold damage feature vector; and 7, analyzing the recovery conditions of the photosynthetic efficiency and the chlorophyll fluorescence, and outputting a cold damage risk grade layer. According to the method, the day and night behavior phase model and the improved TimesNet model are fused, and intelligent risk grading of rice cold damage is achieved.

The present invention relates to biological clocks for measuring skin aging based on the protein expression level measured in a sample of a person's stratum corneum.

The invention discloses a child patient sleep aiding system based on a light adjustment remodeling biological clock, and belongs to the field of medical health. A child patient sleep aiding system based on a light adjustment remodeling biological clock comprises an optical adjustment module, an environment monitoring module, a physiological monitoring module and a central processing module. The intelligent sleep-aiding system solves the problems that an existing sleep-aiding product for children lacks an optical adjustment means for a biological clock, and intervention is inaccurate and child patient compliance is low due to the fact that light environments are changeable and contact type monitoring is uncomfortable. The intelligent sleep-aiding system integrates intelligent phototherapy, ambient light dynamic compensation and non-contact physiological monitoring into a whole, and the intelligent sleep-aiding system has the advantages that the intelligent sleep-aiding system is simple in structure, convenient to use and high in practicability. Individualized illumination intervention can be automatically provided according to individual features and real-time states of child patients so as to improve child sleep disorders, accurate phototherapy dosage is ensured through a spectrum adjustable light source and real-time calibration, sleep physiological signals are analyzed through fusion of multiple sensors, dynamic optimization of intervention parameters is achieved, and the accuracy of the sleep physiological signals is improved. Therefore, the biological clock of the child patient is safely and effectively rebuilt.

The present invention provides agents and other devices that can advance the phase of the circadian rhythm. [Solution] The body clock regulator contains bergamot oil, geranium oil, and cedarwood. It contains at least one of Virginia oil, citral, and β-caryophyllene. It contains an active ingredient that advances the phase of the expression rhythm of clock genes.

The invention relates to the field of biological medicine, in particular to application of butenoid in preparation of a medicine for improving biological clock disorder. According to the application disclosed by the invention, the purple rivet can be used for remarkably inhibiting rhythm oscillation of biological clock core genes Bmal1 and Per2 in two reporter cell lines of Bmal1-luc U2OS and Per2-luc U2OS cells in a dose-dependent manner, so that the period of the rhythm oscillation is prolonged; different T cycles are stimulated by dexamethasone, and the range of U2OS cells adapting to different cycles can be expanded by butenogen. In-vivo experiments show that butein can obviously accelerate the speed of adjusting the time difference of + 6h,-6h and 12h of a mouse. Therefore, the invention provides a new application for butenogenin and expands the application field of butenogenin.

The invention relates to an application of longishin (CRS, C17H16N2O2S) in preparation of a medicine for preventing or treating a time difference syndrome, and belongs to the technical field of biological medicines. The invention finds that the longiday element can adjust the biological clock phase of the zebra fish at 0.3-80 [mu] M. Furthermore, the action mechanism of the longdate element in the period and phase of the biological clock is defined, the longdate element is found to adjust the phase of the biological clock instead of mainly aiming at sleep, the compound has more and more obvious phase adjustment along with the increase of concentration, and the compound is especially suitable for people who intercontinental cross-time-zone travel, and has a wide application prospect. And the internal biological clock can be properly adjusted by adopting corresponding concentration according to the change amount of the time zone, so that the clock can quickly adapt to the local time. Meanwhile, the biological clock regulation function of the longbiantin is reversible, the longbiantin has the regulation function after being taken, and the longbiantin recovers to a normal biological clock after being canceled.

The invention discloses a composite plant composition for synchronizing and magnifying skin circadian rhythm, which comprises a myrothamnus flabellifolia leaf / stem extract, a crithmum maritimum extract, a Thermus thermophilus fermentation product and a broad-leaved epiphyllum callus extract in a weight ratio of (1-3): (0.1-0.5): (0.5-4): (0.5-10). The myrothamnus flabellifolia leaf / stem extract, the crithmum maritimum extract, the Thermus thermophilus fermentation product and the broad-leaved epiphyllum callus extract are combined to act, so that the disordered skin biological clock can be reset, and the time rhythm can be synchronized. And on the basis of rhythm synchronization, the nighttime repair capability of the skin can be remarkably amplified, DNA damage repair is accelerated, barrier related protein synthesis is promoted, and the cell renewal rate is increased.

Provided is a compound that exhibits a circadian rhythm regulating action by transmucosal intake, particularly oral intake. Also provided is a transmucosal intake agent comprising at least one selected from the group consisting of a compound represented by general formula (1), a salt thereof, and solvates of the same.

The present invention provides a method for generating a biological clock containing a DNA methylation map applicable to at least two different sample types, the method comprising: (i) providing a first set of DNA methylation maps generated from at least two different sample types from multiple subjects; (ii) generating a composite DNA methylation map from the first set of DNA methylation maps, wherein the composite DNA methylation map contains methylation sites having a matching state in the different sample types; and (iii) generating a biological clock using the composite DNA methylation map with a reference DNA methylation map from one of the at least two sample types.

The invention discloses a biological clock protein Cry-melatonin combined medicine as well as a preparation method and application thereof, and belongs to the field of biological medicines. The core components of the combined drug comprise a recombinant expression vector containing a biological clock protein Cry gene and melatonin; the preparation process comprises preparation of the recombinant expression vector containing the biological clock protein Cry gene, preparation forming and combined packaging. According to the combined medicine disclosed by the invention, through the synergistic effect that the Cry recombinant expression vector regulates MT1 / 2 receptor expression and melatonin inhibits a TLR4 / NF-kappa B inflammation pathway, mRNA and protein expression of inflammatory factors can be remarkably reduced, testis-epididymis pathological injuries can be repaired, spermatogenesis related gene expression can be up-regulated, so that the sperm malformation rate is reduced, the sperm quality is recovered, and the curative effect is good. The invention has the advantages of high safety and no drug resistance risk, can be applied to the treatment of testis-epididymitis of male mammals, provides potential technical reference for the treatment of human male reproductive system inflammation, and has important significance in the fields of endangered animal protection, animal husbandry development and reproductive medicine.

This invention relates to a computer-implemented method for establishing an epigenetic clock for avian species, the method comprising (a.) identifying and determining the methylation level of specific CpG sites in genomic DNA obtained from multiple different biological sample materials from avian species and representing specific time points within the lifespan of that avian species, (b.) excluding all CpG sites associated with single nucleotide polymorphisms (SNPs) from the CpG sites identified in step (a.), (c.) excluding all CpG sites located on sex chromosomes (Z and W) from the CpG sites obtained in step (b.), (d.) performing a tissue-specific normalization step on the CpG sites obtained in step (c.), and (e.) using a penalized regression model to correlate the CpG methylation level of the CpG sites obtained in step (d.) with actual age.

The invention discloses an artificially optimized soybean biological clock rhythm specific enhancer-promoter element, and belongs to the technical field of genetic engineering. The technical problem to be solved by the invention is how to realize specific regulation and control of expression rhythm and expression quantity of target genes in soybeans. In order to solve the technical problem, the invention provides a nucleic acid molecule, and the nucleic acid molecule can be double-stranded DNA containing ACR1 and ACR2. ACR1 or / and ACR2 is expected to be used for genetic engineering breeding of soybeans, and specific regulation and control of expression rhythm and expression quantity of target genes in the soybeans are realized.

The present application relates to the technical field of medical typing decision, in particular to a myocardial infarction metabolic rhythm intelligent typing and dosing decision method and system, comprising: synchronously detecting biological clock gene BMAL1 mRNA, USP2-BCAT2 complex and branched chain amino acid BCAAs through a multi-index joint detection test box; collecting BCAAs circadian rhythm curve through a wearable monitoring patch integrating a sweat BCAAs sensor and an electrocardio monitoring module; and utilizing a random forest model myocardial infarction metabolic rhythm typing intelligent model. The present application establishes a multi-dimensional dynamic typing system by integrating BMAL1 methylation, USP2-BCAT2 complex and BCAAs rhythm fluctuation, accurately distinguishes high-risk subtypes requiring metabolic intervention, early warns metabolic abnormal state through real-time monitoring of BCAAs concentration fluctuation by an intelligent patch, and automatically optimizes dosing timing based on individual biological clock phase, thereby solving the clinical pain points of insufficient typing precision, abnormal early warning lag and dosing timing mismatch.