103 results about "Salinomycin" patented technology

The invention relates to a beaver rabbit feed, which belongs to the feed field. The beaver rabbit feed mainly comprises the components of robenidine hydrochloride, monensin, salinomycin, bacitracin, garlicin, feed yeast, feed grade magnesium sulphate, feed grade DL-methionine, feed grade L-lysine hydrochloride, common salt, sulfur, calcium hydrophosphate, copper element, zinc element, iron element, choline chloride and vitamin complex premix, etc. The beaver rabbit feed can ensure that the conception rate of female beaver rabbits is improved to more than 93 percent and even to 100 percent; by adopting the beaver rabbit feed, the survival rate at 60 days of the beaver rabbits in the growing period can be improved to about 95 percent, and the growth cycle can be reduced by about half a month; simultaneously the growth of the hair of the beaver rabbit can be promoted, and the density, thickness uniformity and color brightness of the hair can be improved.

The invention relates to a biosynthetic gene cluster of salinomycin and application of the biosynthetic gene cluster of the salinomycin and specifically provides clone, sequencing, analysis, function researches and the application of the biosynthetic gene cluster of antibiotic-salinomycin which is generated by streptomyces albus and has coccidium inhibitory activity and growth promotion activity. The whole gene cluster comprises 30 genes, wherein 17 of the genes are structural genes biosynthesized by the salinomycin, one of the genes is an adjustable gene biosynthesized by the salinomycin, two of the genes are transport relevant genes, other genes are 10 in number, and the 10 genes are slnM, orf11, orf12, orf13, orf14, orf15, orf16, orf17, orf18 and orf19. The genes provided by the biosynthetic gene cluster of the salinomycin and the application of the biosynthetic gene cluster of the salinomycin and protein of the genes can be used for searching and finding out chemical compounds, genes or protein which are used in medicines, industry or agriculture.

The invention discloses an application of salinomycin in the preparation of medicaments for resisting various human malignant tumors. Salinomycin adopted by the present invention assists in inhibiting the growth of a plurality of human tumor cells and metastatic tumor cells. Further, salinomycin is capable of killing a plurality of tumour stem cells such as lung cancer stem cells. Salinomycin haspotentials to be adopted in the exploitation of anticancer medicaments aimed at tumour stem cells, and has important values in the exploitation of medicaments for resisting various human malignant tumors such as lung cancers, skin cancers, pancreas cancers, colon cancers, liver cancers, rectal cancers, brain cancers, renal cell carcinoma, brain-metastatic prostate cancers, and the like.

The invention relates to a processing process for a salinomycin granular preparation. The processing process includes the following steps that acid fermentation liquor is prepared; the pH value of the salinomycin fermentation liquor is adjusted to range from 4.0 to 5.0 through acid liquor; the acid fermentation liquor is pre-treated, wherein the acid fermentation liquor with the adjusted pH value is subjected to heat preservation for 3-4 hours at the temperature of 60 DEG C to 65 DEG C; the pH value is adjusted into an alkaline state for saponification, and salinomycin feed liquor is prepared, wherein a certain amount of light calcium carbonate is added to the obtained acid fermentation liquor to prepare the salinomycin feed liquor; spray drying is conducted, wherein after spraying particles are added to the prepared salinomycin feed liquor, the salinomycin feed liquor is prepared into particles through a spray drying method, the particles are screened, the particles and auxiliary materials are packaged in a mixed mode, and then the salinomycin granular preparation is obtained. The problems of the low yield and the unideal spraying effect caused by an existing salinomycin granular preparation process are solved.

The invention discloses an environment-friendly ecological broiler feed which comprises, by weight, 528 parts of corn, 100 parts of wheat, 275 parts of 46% soybean meal, 5 parts of soya-bean oil, 50 parts of 60% high protein powder, 15 parts of stone flour, 3 parts of salt, 13.5 parts of calcium hydrogen phosphate, 1.3 parts of 99% methionine, 4.05 parts of 65% lysine, 0.25 part of anti-oxidants, 0.31 part of vitamin for chicken, 0.13 part of colistin sulfate, 0.11 part of phytase, 0.9 part of probiotics, 0.15 part of kemzyme, 2.5 parts of choline, 0.5 part of salinomycin, 1 part of broiler multi-minerals, 0.5 part of 10% bacitracin zinc, and 0.5 part of Youpuding. The breeding method using the broiler feed is a broiler breeding method for reducing broiler manure nitrogen phosphorus discharge; because of the addition of phytase and probiotics in the feed, the nitrogen and phosphorus utilization rates of broilers are effectively improved, the excretion amounts of nitrogen and phosphorus are decreased, and the utilization rate of the feed is improved.

The invention relates to a preparation method for a salinomycin granular preparation. The preparation method includes the following steps that acid fermentation liquor is prepared, wherein the pH value of the salinomycin fermentation liquor is adjusted to range from 4.0 to 5.0 through acid liquor; the acid fermentation liquor is pre-treated, wherein the acid fermentation liquor with the adjusted pH value is subjected to heat preservation for 3-4 hours at the temperature of 60 DEG C to 65 DEG C; salinomycin feed liquor is prepared, wherein a certain amount of light calcium carbonate is added to the obtained acid fermentation liquor to prepare the salinomycin feed liquor; spray drying is conducted, wherein the prepared salinomycin feed liquor is prepared into particles through a spray drying method, and then the salinomycin granular preparation is obtained. Acid fermentation is adopted in the salinomycin fermentation process, the layering speed of the fermentation liquor is increased, the layering time is shortened, layering is complete, the yield of salinomycin is increased, the spray pelleting is added and matched with set spraying conditions, spraying efficiency is improved.

The invention provides a phosphoryl methyl salinomycin ether derivative, which can be prepared by the following steps: conversing a hydroxyl and a halogeno methyl alkyl sulfur compound of site 9 and site 20 in a salinomycin compound into alkyl sulfide methyl ether so as to generate an alkyl sulfide methyl salinomycin ether compound; reacting with SO2Cl2 to generate halogeno methyl salinomycin ether; then reacting with phosphoryl-protecting group to generate a phosphoryl methyl salinomycin ether with the protecting group; and then conducting catalytic hydrogenation, adding alkali or amine, thus preparing the phosphoryl methyl salinomycin ether derivative. The phosphoryl methyl salinomycin ether derivative has good water-solubility and can be converted into salinomycin in blood and effectively kill cancer stem cells, thus achieving the treatment purposes of effectively killing cancer cells, preventing cancer reoccurrence and cancer metastasis. A preparation method of the invention has moderate reaction conditions, high yield, easy operation and capability of industrialized production. The structural general formula of the phosphoryl methyl salinomycin ether derivative is shown general formula (I).

The invention discloses an elastic targeting polypeptide-based medicine-carrying nanoparticle. The nanoparticle is prepared from an elastic targeting polypeptide and a modified medicine, wherein the modified medicine is a modified paclitaxel PTX-LEV-MECH or modified salinomycin Sail-ABA-MPBH. The elastic targeting polypeptide-based medicine-carrying nanoparticle provided by the invention is 100nmor below in particle size, so that the dispersion degree is low, the medicine carrying rate is high, and the nanoparticle can be combined with in-vivo albumin for transferring and carrying medicine, and also can be combined with acidic rich cysteine specifically secreted by tumor cells, the drug is concentrated in the acidic environment, the breast cancer in-situ cancer is targeted for killing, and the toxic and side effects of the drug on the whole body are reduced; and the efficacy of treating breast cancer is increased and improved by utilizing the synergistic effect of two nanoparticles, the occurrence of the metastasis of the breast cancer cells through a lymphatic system and a blood system is reduced, and the occurrence of serious complications such as medical-source lymphatic edemacaused by a lymph node sweeping surgery is reduced.

The invention discloses a method for improving the fermentation level of salinomycin by increasing supply of precursors. The method comprises the following steps: doubling acetyl coA carboxylase genes from streptomyces coelicolor, methyl malonyl coA mutase genes from streptomyces albus DSMZ41398 and crotonoyl coA reductase genes from the streptomyces albus DSMZ41398 on the chromosomes of the streptomyces albus DSMZ41398 through an integrating vector pSET152 respectively to increase supply of three precursors required for synthesizing salinomycin so as to increase the salinomycin yield. According to the method, the final fermentation yield of engineering strain salinomycin is increased by 260% and the shake flask level of a lab reaches 2 g/L.

The invention provides the application of salinomycin and derivatives thereof in preparing drugs for preventing or treating brain glioma, relates to the new pharmaceutical use of known salinomycin and derivatives thereof and develops a new application area. The salinomycin and derivatives thereof show strong pharmacological actions, have a strong effect of selectively killing brain glioma stem cells, and have good pharmaceutical prospects.

The invention provides a phosphoryl carboxylic acid salinomycin ester derivative having a structure as stated in the lower right general formula (I): ensuring that salinomycin compound reacts with (a) at the existence of alkali to obtain corresponding ester; reacting with (b) to obtain a target product (I); or ensuring that salinomycin (a) reacts with (c) at the existence of alkali to obtain corresponding ester (c); and performing catalytic hydrogenation to obtain a target product. The derivative of the invention has favourable water solubility and can be prepared into injection, and the derivative is converted into salinomycin in blood, the prepared preparation has the characteristics of high bioavailability, fast adsorption, favourable stability and the like, and can effectively kill cancer stem cells. The preparing method of the invention has high yield, simple operation and industrial production foreground. The general formula (I) structure of the invention is disclosed.

The invention belongs to the technical field of veterinary drug residue analysis and discloses a high performance liquid chromatogram pre-column derivatization reaction analysis method for a hydrogen substitution derivatization reaction of carboxyl compounds of polyetherionophores. Carboxyl groups contained in polyetherionophores react with a fluorescent reagent of 1-(bromoacetyl) pyrene, and the products have extremely-high fluorescence. The following reaction conditions are optimized: a 18-crown ether-6 solution is used as a phase transfer catalyst; a 1-(bromoacetyl) pyrene solution is used as a derivatization reagent; the reaction is conducted in a water bath at 30-70 DEG C for 150 min; after being cooled, the products are detected through a high performance liquid chromatograph. The excitation wavelengths of the derivatives are 360 nm; the derivatives have the highest fluorescence intensity when the emission wavelengths of the derivatives are 420 nm; the minimum detectable amount is 50 Mug/L; the derivatives show linear response to peak areas within the range of 200-1600 [mu]g/L; the result reproducibility is high; the variable coefficient of 3 concentrations and 15 repetitions is smaller than 7.8%; all indexes can meet the residue analysis requirements of the three polyetherionophores of monensin, salinomycin and lasalocid.

The invention relates to a salinomycin granule preparation preparation technology based on improvement of the spraying effect. The preparation technology includes the following steps that acidic fermentation liquid is prepared, wherein the pH of salinomycin fermentation liquid is adjusted to 4.0-5.0 through acidic liquid; the acidic fermentation liquid is pre-processed, wherein the temperature of the acidic fermentation liquid with the pH adjusted is preserved for 3-4 h at the temperature of 60-65 DEG C; the pH is adjusted till the salinomycin fermentation liquid is alkaline, a saponification reaction is performed, and salinomycin material liquid is prepared, wherein a certain amount of light calcium carbonate is added into the prepared acidic fermentation liquid so that salinomycin material liquid can be prepared; spraying and drying are performed, wherein after spraying particles are added into the prepared salinomycin material liquid, granules are prepared through a spraying and drying method, and then a salinomycin granule preparation is obtained. The salinomycin granule preparation preparation technology solves the problems that an existing salinomycin granule preparation technology is low yield and not ideal in spraying effect.

The invention relates to a piglet concentrated forage. The piglet concentrated forage comprises 43% soybean meal, puffed soybean powder, corn oil, steamed fish meal, puffed corn, milk replacer, 98% lysine, methionine, choline chloride, a sweetening agent, a flavoring agent, white sugar, youtianbao, calcium biphosphate, TP100, zinc oxide, salt, pig multivitamins, pig microelements, colistin sulfate, lysozyme, salinomycin and an antioxidant. The piglet is fed by mixing the piglet concentrated forage as a fine feed and a farmer self-prepared coarse feed according to a proportion of 1:1. The forage is relatively low in cost, has good palatability, has a relatively good feed effect and is welcome to pig-breeding farmers. The piglet fed by the forage has relatively obvious daily weight increase and relatively low diarrhea rate.

The present invention discloses a method for detecting salinomycin and its special-purpose ELIA kit. Said kit includes salinomycin specific antibody, coating source and enzyme label. The described coating source is conjugate of salinomycin semiantigen and carrier protein or antiantibody, the described enzyme label is enzyme-labelled antiantibody or enzyme-labelled salinomycin semiantigen. When the described coating source is the conjugate of salinomycin semiantigen and carrier protein, the described enzyme label is enzyme-labelled antiantibody, and when the described coating source is antiantibody, the described enzyme label is enzyme-labelled salinomycin semiantigen.

The present invention discloses a fermented goat feed, comprising the following raw materials in parts by weight of 53 to 60 parts of grass meal, 20 to 25 parts of soybean cake, 8 to 10 parts of seaweed meal, 8 to 10 parts of wheat bran, 3 to 4 parts of folium isatidis, 2 to 4 parts of sweetberry jointfir, 2 to 4 parts of grape pip, 1 to 2 parts of lygodium japonicum, 1 to 2 parts of crushed Mongolian snakegourd shell, 1 to 2 parts of isatis root, 2 to 3 parts of compressed biscuit additive, 0.1 to 0.3 parts of salinomycin, 0.08 to 0.1 parts of methionine and 0.001 to 0.0012 parts of sodium selenite. Feed provided by the present invention contains various raw materials required for the goat production, which not only ensure health production of the goat, but provide health care value, and the method of mixed bacteria liquid fermentation increases the shelf life of feed, and a plurality of probiotics promote normal growth of the goat and improve disease resistance, and the added biscuit additives further complement a variety of nutrients needed for the goat growth, and the goat chews more the feed in the form of compressed biscuit, while the goat chews less the traditional goat feed.

The invention discloses a method for extracting salinomycin compounds from an animal sample and a special immunoaffinity absorbent thereof. The immunoaffinity absorbent for extracting the salinomycin compounds from the animal sample consists of a solid phase carrier and a salinomycin monoclonal antibody which is coupled with the solid phase carrier; the salinomycin monoclonal antibody is an antibody obtained by taking a conjugate of salinomycin semiantigen and carrier protein as immunogen; and the salinomycin compounds are salinomycin or methylsalinomycin. The immunoaffinity absorbent and a chromatographic column use the high specific salinomycin monoclonal antibody, have high selectivity, ensure the reliability of detection effect, greatly simplify the pretreatment processes of samples at the same time, are particularly applicable to the pretreatment of a trace amount of salinomycin and methylsalinomycin in muscle and liver, and improve the analysis quality. A detection method of the invention can efficiently detect the content of the salinomycin and the methylsalinomycin, and remedy the disadvantages that the direct assay of the samples by a single immunoassay technology has less information amount, poor quantification accuracy, or low selectivity of physical and chemical methods and so on, so the immunoaffinity absorbent, the chromatographic column, a reagent kit and the methods for extracting and detecting the salinomycin compounds in the animal sample are suitable to be promoted and applied.

The invention discloses a long-hair-rabbit biological feed formula and a preparation method thereof. According to the formula, the long-hair-rabbit biological feed comprises the following components in parts by mass: 15-20 parts of bean cake, 1-2 parts of fish meal, 1-2 parts of bone powder, 10-15 parts of bran, 0.6-0.9 part of table salt, 0.8-1 part of astragalus membranaceus, 40-45 parts of grass meal, 6-8 parts of rice polishing, 1-2 parts of dry distillers' grains, 0.8-1 part of isatis root, 0.6-0.9 part of cyrtomium fortune, 0.4-0.7 part of fructus forsythiae, 0.8-1 part of hymenasplenium cardiophyllum, 0.9-1.3 parts of pteris multifida poir, 0.4-0.5 part of cortex moutan, 100-150 parts of corn, 0.08-0.1 part of methionine, 0.1-0.3 part of salinomycin and 0.06-0.08 part of lysine. By adoption of the formula, the hair color of long-hair rabbits is brighter, diseases of long-hair rabbits can be reduced; raw materials in the formula of the feed are abundant, and thus the formula is economical and practical.

The invention discloses big meat chicken feed containing 4% of premix. The big meat chicken feed containing 4% of premix comprises 4% of premix, and is composed of the following components in parts by weight: calcium hydrogen phosphate, stone powder, salt, iron, copper, zinc, manganese, potassium iodide, sodium selenite, vitamin A, vitamin D3, vitamin E, vitamin K3, vitamin B1, vitamin B2, vitamin B6, vitamin B12, nicotinic acid, biotin, pantothenic acid, folic acid, salinomycin, sweetening agent, wheat bran powder, and a proper amount of zeolite powder. Big meat chickens fed with the big meat chicken feed containing 4% of premix are low in the feed to gain ratio (the feed conversion rate is high), and the death rate is low, so that the breeding cost is reduced, and the breeding benefit is improved.

The invention discloses a solid-liquid separation method of salinomycin fermentation liquor. The method comprises the following steps of: firstly, raising the temperature of the salinomycin fermentation liquor to 55 to 65 DEG C by heating; during stirring, adding 30 percent of caustic soda liquid of an ionic membrane to adjust a potential of hydrogen (pH) value to 10.0 to 11.0; adding concentrated sulfuric acid to adjust the pH value to 7.0 to 8.0; adding calcium carbonate with 3 volume percent of fermentation liquor and sodium silicate with 6 volume percent of fermentation liquor; stirring for 2 hours to obtain salinomycin bacteria grout; and under the squeezing pressure of 1.6 MPa, performing membrane filter pressing on the salinomycin bacteria grout to obtain a salinomycin filter cake solid. With the characteristic that the salinomycin fermentation liquor is subjected to alkaline hydrolysis and then subjected to solid-liquid separation by using a plate frame, a salinomycin filter cake having lower water content is produced. The solid-liquid separation method of the salinomycin fermentation liquor has the advantages of high solid-liquid separation yield, low concentration of a chemical oxygen demand (COD) in waste liquor, low water content of the filter cake, and low production cost; and the cost of environmental protection is reduced.