The invention discloses a flotation separation technology for high-sulfur lead-zinc ore in a high-concentration environment. The flotation separation technology aims at high-sulfur lead-zinc sulfide ore containing associated gold and silver, and flotation of lead and associated gold and silver minerals is preferentially carried out in a high-concentration and neutral natural pH flotation environment; and lead-flotation tailings are subjected to two-stage rough flotation so as to realize bulk flotation of zinc-sulfur minerals, the pH value and the potential of ore pulp are regulated and controlled by adopting lime in the zinc-sulfur separation flotation process so as to realize zinc-sulfur separation, and a small amount of activating agents and collecting agents are added to strengthen flotation of zinc minerals difficult to float. According to the flotation separation technology, the defects that in the lead flotation process of high-alkali mediums, the recovery rate of lead, gold andsilver is low, the usage amount of agents is large, the floatation process is not stable, and the like can be overcome; and meanwhile, a zinc-sulfur mineral inhibitor XKK012 and a lead-gold-silver collecting and frothing agent XKY712 are invented, so that the problems that the lead-zinc-sulfur separation efficiency is low, the separation precision is poor, and the hydrophobic capacity of part of lead-gold-silver minerals is weak can be solved, and the technology has the advantages of being energy-saving, environment-friendly, non-toxic, harmless, low in beneficiation cost, high in recovery efficiency and suitable for comprehensive recovery of valuable metal minerals in the high-sulfur silver-lead-zinc-contained ore.