Method for preparing salinomycin particle premix
A salinomycin and premix technology, which is applied in the field of preparation of salinomycin granule premix, can solve the problems of salinomycin premix not meeting requirements, long production cycle, complicated process, etc., and achieve energy saving uniformity and stability, low oil content, the effect of ensuring stability
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Embodiment 1
[0014] Embodiment 1 content is the preparation method of the salinomycin granule premix of 16.5%
[0015] 1 Bacterial culture of salinomycin
[0016] Select a few single colonies with a plump appearance from the slant, and put the colony spores into 5ml sterile water and shake them evenly under aseptic conditions. Apply the spore suspension evenly on the spore-producing slant medium by streaking method and culture in a 30°C constant temperature room for 8 days. Mark the well-grown slant spore bottle with the strain number, the date of the next bottle, and store in a refrigerator at 2-4°C spare.
[0017] 2 Fermentation production
[0018] Add 30ml of water to each slant of the slant spores, scrape the spores carefully so that they do not carry any medium substances, put them together and pour them into a sterile bottle, and then inoculate them into a 500L first-level seed tank by the differential pressure method. After 30 hours, the mycelia Dense network segmented hyphae are...
Embodiment 2
[0025] Embodiment 2 content is the preparation method of the salinomycin granule premix of 31%
[0026] 1. Bacterial culture of salinomycin
[0027] Select a few single colonies with a plump appearance from the slant, and put the colony spores into 5ml sterile water and shake them evenly under aseptic conditions. Apply the spore suspension evenly on the spore-producing slant medium by streaking method and culture in a 30°C constant temperature room for 8 days. Mark the well-grown slant spore bottle with the strain number, the date of the next bottle, and store in a refrigerator at 2-4°C spare.
[0028] 2. Fermentation production
[0029] Add 30ml of water to each slant of the slant spores, scrape the spores carefully so that they do not carry any medium substances, put them together and pour them into a sterile bottle, and then inoculate them into a 500L first-level seed tank by the differential pressure method. After 32 hours, the mycelia Dense network segmented hyphae are...
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