38 results about "Streptomyces albus" patented technology

The invention relates to a mutagenized strain of streptomyces albus TUST2, and a method for using the same to produce epsilon-polylysine and a salt of the epsilon-epsilon. The strain is a high-yield mutagenized strain of epsilon-polylysine selected and bred by means of ultraviolet mutagenesis, ultraviolet-chemical mutagenesis, N ion injection mutagenesis, etc from a strain of streptomyces albus TUST2 which is capable of producing epsilon-polylysine and is screened from the earth in Hainan province of China; the mutagenized strain is resistant to 10mg/ml or above S-(2 Aminoethyl )-L-Cysteine and can generate 10 to 30 g/L acid under optimal conditions; zymotic fluid is removed of the streptomyces albus by configuration and is filtered by diatomaceous earth to obtain clear filtrate and to weak acid type ion exchange resin to obtain coarse epsilon-polylysine products which is subjected to ultrafiltration and purification to get epsilon-polylysine and the salt of the epsilon-epsilon. The distribution of molecular weight of epsilon-polylysine is between 4000 and 6500Da.

The invention discloses an environmentally-friendly composite microorganism preparation for eliminating house-fitment pollution. The composite microorganism preparation comprises the following bacterial components in percentage by weight: 15 to 35 percent of lactobacillus acidophilus, 15 to 35 percent of candida utilis, 5 to 25 percent of bacillus licheniformis, 10 to 25 percent of streptomyces albulus and 15 to 30 percent of rhodopseudomonas palustris and is obtained by compositely culturing the bacterial components by a fermentation process which combines anaerobic fermentation with aerobic fermentation.

The invention relates to an epsilon-polylysine high-yielding strain and an epsilon-polylysine production method, and belongs to the biotechnical field. The epsilon-polylysine high-yielding strain EA-19 is named as Streptomyces albus, and the preservation number is CGMCC No.10156. The strain EA-19 is used to carry out fed batch fermentation culture, and the concentration of epsilon-polylysine in a fermentation liquid reaches 38.5-39 g/L. The epsilon-polylysine production method comprises is characterized in that the strain EA-19 is adopted to carry out fermentation culture. A bubbling technology is adopted, ventilation is carried out in and after the fermentation culture process, so epsilon-polylysine generated after fermentation exists in foams, and is separated from the fermentation liquid, so the technical problem of difficulty separation of epsilon-polylysine in present preparation methods is solved, a step of ion exchange which causes generation of a large amount of wastewater is omitted, and the product yield is improved by 50% or above. The method has the advantages of simple process, easiness in amplification, low energy consumption, small device investment, and easiness in realization of large-scale production.

The invention discloses a streptomyces calculus genetically engineered bacterium PD-4. An ammonium transporter gene amtB from an S.albulus PD-1 genome is subjected to overexpression, and polylysine (epsilon-poly-L-lysine, epsilon-PL) synthesis ability higher than that of streptomyces albus S.albulus PD-1 is achieved. The sequence of the ammonium transporter gene is shown as SEQ ID No:1. The invention further discloses a construction method and fermentation verification of the recombinant strain. By means of efficient expression of amtB, limitations caused by defects of ammonium transporters are eliminated, and the rate of a nitrogen source in fermentation liquid utilized by S.albulus PD-4 is increased, so that the yield of epsilon-PL is increased, production cost is reduced, and huge economic benefits are brought.

The invention relates to a streptomyces albus X-18 and method for producing epsilon-polylysine by using the same, and belongs to the technical field of bioengineering. The streptomyces albus X-18 is separated and screened from soil and is preserved, and the preservation number is CGMCC No.17536. The streptomyces albus X-18 is used for producing the epsilon-polylysine, a fermentation culture mediumand fermentation conditions are improved, and intermediate material supplementation and a method for adding inducers are also used so that the fermentation yield of the epsilon-polylysine can reach 3.5-4.2 g/L. A strain with the independent intelligent property right is mainly used, and corn starch low in price is used as the main component of the fermentation culture medium, so that the production cost is reduced, and the fermentation yield is improved.

The invention discloses a compound microbial agent for anaerobic digestion treatment of sludge and a production method of the compound microbial agent. The compound microbial agent is a liquid microbial agent which is prepared through fermentation culture and comprises the following bacteria: bacillus subtilis, streptomyces albus, streptomyces griseus, candida utilis, aspergillus sojae, lactobacillus bulgaricus, lactococcus lactis, rhodopseudomonas palustris, rhodopseudomonas capsulate and rhodobacter sphaeroides, and the number of total viable bacteria in 1ml of the compound microbial agent is not less than 2*10<8>. All the bacteria used by the compound microbial agent have metabiosis and symbiosis effects and form a biodegradation system with very high enzymatic activities. When the compound microbial agent disclosed by the invention is used for carrying out anaerobic digestion treatment on sludge, the degradation rate of organic matters can be effectively increased, the sludge hydrolytic process can be accelerated, the anaerobic digestion time of the sludge can be shortened, and the biological gas yield can be increased.

The invention discloses a method for producing a seedling raising substrate by using cotton straw and a substrate prepared with the same. The method comprises the following steps of: smashing cotton straw into sections of 10-50 millimeters; adding water till the water content is 60-80 percent according to mass percentage; adding 2-3kg of urea, 0.5-1kg of calcium superphosphate, 1-2kg of gypsum and 50-100g of fermentation inoculum into each cubic meter of substances obtained in the last step according to the volume ratio; mixing uniformly; and sealing, piling and fermenting at the air temperature of 15-40 DEG C, wherein the adopted compound fermentation inoculum is prepared by mixing pseudomonas aeruginosa, streptomyces albus and flavobacterium in the ratio of 2:3:1, and can be used by fermenting, airing in a scattered way and drying. In the method, a main raw material is derived from a large amount of waste cotton straw generated during the production of cotton, the raw material source is rich, a preparation process is simple, the aim of utilizing resources is fulfilled, and resource waste of cotton straw caused by direct smashing and returning to field can be reduced; and the method has a wide application value.

The invention discloses a pretreatment method for enhancing anaerobic sludge digestion by virtue of a compound microorganism bacterium agent. The pretreatment method comprises the following steps: concentrating residual sludge, adding a compound microorganism bacterium agent, performing stirring reaction, inoculating the pretreated residual sludge into an anaerobic digestion pond according to a certain ratio, and performing mesophilic anaerobic digestion on anaerobic sludge, wherein the compound microorganism bacterium agent is prepared by mixed fermentation of 5%-20% of each of bacillus subtilis, streptomyces albus, streptomyces griseus, candida utilis, aspergillus sojae, lactobacillus bulgaricus, lactococcus lactis, rhodopseudomonas palustris, rhodopseudomonas capsulate and rhodopseudomonas spheroides, the total viable count is not less than 2*108/ml, and the pH value of the compound microorganism bacterium agent is 3-4. According to the method, the sludge hydrolysis process can be accelerated, the sludge retention time can be shortened, the biological gas production rate can be increased, and the anaerobic digestion efficiency can be improved.

The invention belongs to the technical field of bio-fertilizer, particularly relates to bio-organic fertilizer prepared from soy protein wastewater sludge, and further discloses a preparation method of the bio-organic fertilizer. The preparation method of the bio-organic fertilizer includes: bacillus stearothermophilus, streptomyces albus, fine candida and staphylococcus epidermidis are used as a composite fermentation microbial agent to perform aerobic fermentation on the protein sludge so as to prepare the bio-organic fertilizer. The prepared bio-organic fertilizer is high in nutritive value and can be used as organic fertilizer.

The invention provides a composite microbial preparation for garbage deodorization and a preparation method thereof. The composite microbial preparation is a reddish brown or brown liquid having fermentation-type sour and has the pH value within 3.4 to 4.0; and the total number of effective viable bacteria in the composite microbial preparation is greater than or equal to 1.0*10<9>CFU/mL, wherein the effective viable bacteria comprise 10<5>CFU/mL to 10<7>CFU/mL of saccharomyces cerevisiae, 10<6>CFU/mL to 10<8>CFU/mL of bacillus subtilis, 10<6>CFU/mL to 10<8>CFU/mL of bacillus coagulans, 10<6>CFU/mL to 10<8>CFU/mL of lactobacillus fermentium, 10<6>CFU/mL to 10<8>CFU/mL of lactobacillus buchneri, 10<5>CFU/mL to 10<7>CFU/mL of enterococcus faecium and 10<4>CFU/mL to 10<6>CFU/mL of streptomyces albus. The composite microbial preparation is complex in composition, stable in structure, nontoxic and harmless and cannot generate side effects. The composite microbial preparation can be used for effectively inhibiting the breeding and the growth of putrefying bacteria, pathogenic bacteria and other spoilage organisms and deodorizing garbage, excrement and sewage so as to reduce pollution and improve the environment.

The invention discloses streptomycete strains and a combined application thereof in prevention and treatment of pepper seedling blight, and relates to the technical field of microorganisms. The streptomycetes disclosed by the invention are separated and obtained from a deep soil sample of 10-15cm collected from the Hangzhou xixi wet land in Zhejiang by adopting a streptomycete separation technology, are identified by microbial taxonomy, are named streptomyces albus (streptomyces albus) 2013 and streptomyces fradiae (streptomyces fradiae) M931,S.albus2013; the streptomyces albus 2013 is preserved at the China Center for Type Culture Collection on June 19, 2013; the preservation number is CCTCC NO:M2013269; the S.fradiae M931 is preserved at the China Center for Type Culture Collection on December 25, 2013; and the preservation number is CCTCC NO:M2013712. The two bacterial strains can be fermented to generate active substances with relatively strong antagonistic effects on rhizoctonia solani kuhn (Rhizoctonia solani Kuhn), and can be used for preventing and treating plant diseases such as the pepper seedling blight; when fermentation products of the two bacterial strains are mixed to prepare the mixed preparation according to the mass percent of 3 to 2, the effect for preventing and treating the pepper seedling blight is more significant in comparison with the effect when two metabolites are respectively used as single preparations.

The invention discloses a pyrazomycin biosynthesis gene cluster, recombinant bacteria and an application of the recombinant bacteria, and particularly discloses cloning, sequencing, analysis and function research for the biosynthesis gene cluster of a natural product pyrazomycin which is produced from pure white streptomyces albus NRRL 3601 and used for tumor treatment as well as an application ofthe biosynthesis gene cluster. The whole gene cluster contains 24 genes. Biosynthesis of pyrazomycin can be blocked or increased through genetic manipulation for the biosynthesis genes. The genes andproteins of the genes can be applied to genetic engineering, protein expression and enzymic catalytic reaction of the compound, and can also be used for searching and discovering compounds, genes andproteins capable of being applied to medicine, industry or agriculture.

The invention relates to a fermented feed without antibiotics for animals and a preparation method of the feed. Grape residues are adopted as main fermentation raw materials and mixed with auxiliary components such as corn residues, cottonseed cakes and bean dregs, citric acid is also added, a compound bacterial liquid composed of lactic acid bacteria, yeast, bacillus subtilis and streptomyces albus which are mixed according to a specific proportion is adopted, and all the materials are fermented in a water content and pH range of a specific fermentation mixture to prepare the fermented feed without antibiotics. After the animals are fed with the feed, the intestinal microbial balance of the animals can be improved, the immunity is enhanced, the growth performance of the animals is improved, the prepared fermented feed can replace the role of antibiotics in feed, and the feed safety problem is effectively solved. The fermented feed which can replace feed antibiotics is prepared by means of the simple preparation method under the optimized fermentation conditions, the cost is low, and industrialization is easy.

The invention discloses engineering streptomycete for producing daunorubicin and a construction method of the engineering streptomycete. Streptomyces albus ZD11 is used as an original strain, and through in-vitro fishing of large fragments of a genome, engineering transformation of a pathway specific activation protein gene promoter, deletion of a synthetic branch and blocking of a competitive synthetic pathway, an engineering streptomycete strain ZD11-M4 capable of heterologously synthesizing the daunorubicin is constructed, and the shake-flask fermentation yield of the daunorubicin reaches 22.62 mg/g and is doubled compared with that of an original strain. According to the engineering streptomycete provided by the invention, heterologous expression of daunorubicin synthetic gene clustersis realized for the first time, and a technical foundation is laid for industrial production of the daunorubicin. Besides, an efficient expression method of a polyketone compound in a heterologous host provided by the invention has important research and application values for exploring novel polyketone antibiotics, reducing the fermentation cost and increasing the yield.

The invention discloses a microbial composition and a method for treating wastewater by using the same. The invention relates to a microbial composition for treating wastewater. The microbial composition comprises at least two of pseudomonas stutzeri, streptomyces rubrum, streptomyces albus, bacillus subtilis, bacillus mucilaginosus and halophilous actinomycetes. The wastewater meets one or two of the following conditions: a, the COD (Chemical Oxygen Demand) value reaches 90000-110000 mg/L; and b, the total nitrogen value reaches 3000 to 5000 mg/L. The method for culturing the biological composition by using the taurine industrial wastewater, provided by the invention, is low in cost and good in effect; the waste liquid in the taurine production is used for culturing the microbial composition, so that COD and total nitrogen in the waste water can be reduced (more than 95% of COD can be degraded at most); and the wastewater can be used for culturing a microbial composition, and bacteria obtained by fermentation can be used as a biological fertilizer and have a fertility increasing effect on vegetables and green plants.

The invention discloses a genetically engineered bacterium of high-yield epsilon-polylysine, namely streptomyces albus pd9-pld3, and the preservation number of the genetically engineered bacterium is CCTCC (China Center for Type Culture Collection) M 20211549. The invention also discloses an application of the engineering bacterium in fermentation production of epsilon-polylysine, and an optimized fermentation culture medium for the streptomyces albus pd9-pld3, and also discloses an application of the engineering bacterium in fermentation production of epsilon-polylysine, and an optimized fermentation culture medium for the streptomyces albus pd9-pld3. The yield of the epsilon-polylysine of the streptomyces albus disclosed by the invention is obviously improved compared with that of an original strain, and the inhibition on an epsilon-polylysine degrading enzyme can be realized.

Disclosed is a high-throughput transcriptional assay format in Actinomycete bacteria, and Streptomyces spp. in particular, that leverages eGFP, inserted both at a neutral site and inside the biosynthetic cluster of interest, as a read-out for secondary metabolite synthesis. Using this approach, a silent gene cluster in Streptomyces albus J1074 was induced. The cytotoxins etoposide and ivermectin were revealed as potent inducers, allowing the isolation and structural characterization of nearly 20 novel small molecule products of the chosen cluster. One of these molecules is a novel antifungal, while several others inhibit a cysteine protease implicated in cancer. Studies addressing the mechanism of induction by the two elicitors led to the identification of a pathway-specific transcriptional repressor that silences the gene cluster under normal growth conditions. The successful implementation of this approach will allow future discovery of cryptic metabolites with useful bioactivities from Actinomycete bacteria.