38 results about "Streptomyces albus" patented technology

The invention relates to a mutagenized strain of streptomyces albus TUST2, and a method for using the same to produce epsilon-polylysine and a salt of the epsilon-epsilon. The strain is a high-yield mutagenized strain of epsilon-polylysine selected and bred by means of ultraviolet mutagenesis, ultraviolet-chemical mutagenesis, N ion injection mutagenesis, etc from a strain of streptomyces albus TUST2 which is capable of producing epsilon-polylysine and is screened from the earth in Hainan province of China; the mutagenized strain is resistant to 10mg / ml or above S-(2 Aminoethyl )-L-Cysteine and can generate 10 to 30 g / L acid under optimal conditions; zymotic fluid is removed of the streptomyces albus by configuration and is filtered by diatomaceous earth to obtain clear filtrate and to weak acid type ion exchange resin to obtain coarse epsilon-polylysine products which is subjected to ultrafiltration and purification to get epsilon-polylysine and the salt of the epsilon-epsilon. The distribution of molecular weight of epsilon-polylysine is between 4000 and 6500Da.

The invention discloses an environmentally-friendly composite microorganism preparation for eliminating house-fitment pollution. The composite microorganism preparation comprises the following bacterial components in percentage by weight: 15 to 35 percent of lactobacillus acidophilus, 15 to 35 percent of candida utilis, 5 to 25 percent of bacillus licheniformis, 10 to 25 percent of streptomyces albulus and 15 to 30 percent of rhodopseudomonas palustris and is obtained by compositely culturing the bacterial components by a fermentation process which combines anaerobic fermentation with aerobic fermentation.

The invention relates to an epsilon-polylysine high-yielding strain and an epsilon-polylysine production method, and belongs to the biotechnical field. The epsilon-polylysine high-yielding strain EA-19 is named as Streptomyces albus, and the preservation number is CGMCC No.10156. The strain EA-19 is used to carry out fed batch fermentation culture, and the concentration of epsilon-polylysine in a fermentation liquid reaches 38.5-39 g / L. The epsilon-polylysine production method comprises is characterized in that the strain EA-19 is adopted to carry out fermentation culture. A bubbling technology is adopted, ventilation is carried out in and after the fermentation culture process, so epsilon-polylysine generated after fermentation exists in foams, and is separated from the fermentation liquid, so the technical problem of difficulty separation of epsilon-polylysine in present preparation methods is solved, a step of ion exchange which causes generation of a large amount of wastewater is omitted, and the product yield is improved by 50% or above. The method has the advantages of simple process, easiness in amplification, low energy consumption, small device investment, and easiness in realization of large-scale production.

The invention provides a high-inorganic nitrogen-sourced biological compound fertilizer and a preparation method thereof. The fertilizer contains the following functional bacteria: bacillus megaterium, bacillus subtilis, saccharomyces cerevisiae, lactobacillus plantarum, Mucor abundans and streptomyces albus. The content of inorganic nitrogen of the compound fertilizer can be as high as 35 percent, while the number of live bacteria can still be up to 2*10 cfu / kg. Experiments show that the field fertilizer efficiency of the compound fertilizer is significantly improved.

The invention discloses a streptomyces calculus genetically engineered bacterium PD-4. An ammonium transporter gene amtB from an S.albulus PD-1 genome is subjected to overexpression, and polylysine (epsilon-poly-L-lysine, epsilon-PL) synthesis ability higher than that of streptomyces albus S.albulus PD-1 is achieved. The sequence of the ammonium transporter gene is shown as SEQ ID No:1. The invention further discloses a construction method and fermentation verification of the recombinant strain. By means of efficient expression of amtB, limitations caused by defects of ammonium transporters are eliminated, and the rate of a nitrogen source in fermentation liquid utilized by S.albulus PD-4 is increased, so that the yield of epsilon-PL is increased, production cost is reduced, and huge economic benefits are brought.

The invention relates to a streptomyces albus X-18 and method for producing epsilon-polylysine by using the same, and belongs to the technical field of bioengineering. The streptomyces albus X-18 is separated and screened from soil and is preserved, and the preservation number is CGMCC No.17536. The streptomyces albus X-18 is used for producing the epsilon-polylysine, a fermentation culture mediumand fermentation conditions are improved, and intermediate material supplementation and a method for adding inducers are also used so that the fermentation yield of the epsilon-polylysine can reach 3.5-4.2 g / L. A strain with the independent intelligent property right is mainly used, and corn starch low in price is used as the main component of the fermentation culture medium, so that the production cost is reduced, and the fermentation yield is improved.

The invention discloses a preparation method of a seedling culture medium of watermelon seedlings. The preparation method comprises the following steps: (1) uniformly mixing straws, rice husks, sawdust, biogas residues, mushroom residues and wine residues to obtain a fermentation medium; (2) adding a fermentation bacterial agent into the fermentation medium, and laminating, composting and fermenting to obtain a composted fermentation product, wherein the fermentation bacterial agent is mixed bacteria of saccharomycetes, actinomycetes, lactobacillus and bacillus subtilis; the saccharomycetes is pichia pastoris, the actinomycetes is streptomyces albus, the lactobacillus is lactobacillus acidophilus and the bacillus subtilis is bacillus vallismortis; (3) mixing the composted fermentation product with pearlite to obtain the seedling culture medium of the watermelon seedlings. The preparation method of the seedling culture medium of the watermelon seedlings has the advantages that the preparation method is simple, the cost is low, the cultured watermelon seedlings are stronger, the quality of the watermelon seedlings is good, the total dry weight of the watermelon seedlings is remarkably improved, the root density is remarkably improved and the like.

The invention discloses a compound microbial agent for anaerobic digestion treatment of sludge and a production method of the compound microbial agent. The compound microbial agent is a liquid microbial agent which is prepared through fermentation culture and comprises the following bacteria: bacillus subtilis, streptomyces albus, streptomyces griseus, candida utilis, aspergillus sojae, lactobacillus bulgaricus, lactococcus lactis, rhodopseudomonas palustris, rhodopseudomonas capsulate and rhodobacter sphaeroides, and the number of total viable bacteria in 1ml of the compound microbial agent is not less than 2*10<8>. All the bacteria used by the compound microbial agent have metabiosis and symbiosis effects and form a biodegradation system with very high enzymatic activities. When the compound microbial agent disclosed by the invention is used for carrying out anaerobic digestion treatment on sludge, the degradation rate of organic matters can be effectively increased, the sludge hydrolytic process can be accelerated, the anaerobic digestion time of the sludge can be shortened, and the biological gas yield can be increased.

The invention discloses a method for producing a seedling raising substrate by using cotton straw and a substrate prepared with the same. The method comprises the following steps of: smashing cotton straw into sections of 10-50 millimeters; adding water till the water content is 60-80 percent according to mass percentage; adding 2-3kg of urea, 0.5-1kg of calcium superphosphate, 1-2kg of gypsum and 50-100g of fermentation inoculum into each cubic meter of substances obtained in the last step according to the volume ratio; mixing uniformly; and sealing, piling and fermenting at the air temperature of 15-40 DEG C, wherein the adopted compound fermentation inoculum is prepared by mixing pseudomonas aeruginosa, streptomyces albus and flavobacterium in the ratio of 2:3:1, and can be used by fermenting, airing in a scattered way and drying. In the method, a main raw material is derived from a large amount of waste cotton straw generated during the production of cotton, the raw material source is rich, a preparation process is simple, the aim of utilizing resources is fulfilled, and resource waste of cotton straw caused by direct smashing and returning to field can be reduced; and the method has a wide application value.

The present invention relates to product(s) containing living microorganism(s) suitable for soil treatment, microorganisms multiplying under different climatic and natural circumstances, as well as procedures for the production of the products, and procedures for the treatment of the soil and plants with the products. More particularly, the invention relates to a procedure for preparing the products from any of the microorganisms specified below, or from the mixture thereof. Furthermore, the invention relates to a procedure for the creation of the cultures of the microorganisms to be used. The subject invention also pertains to the microorganisms themselves. More particularly, the invention relates to a procedure for the treatment of the soil and the plants with a product containing at least one of the microorganisms selected from Azospirillum brasilense ssp. SW51 (NCAIM / P / B 001293), Azotobacter vinelandii ssp. M657 (NCAIM / P / B 001292), Pseudomonas fluorescens var. SW11 (NCAIM / P / B 001296), Bacillus polymyxa var. SW17 (NCAIM / P / B 001295), Bacillus megaterium var. M326 (NCAIM / P / B 001291), Micrococcus roseus ssp. A21 (NCAIM / P / B 001294), Bradyrhizobium japonicum var. PH25 (NCAIM / P / B 001302), and Streptomyces albus var. 0003 LP (NCAIM / P / B 001301), and furthermore the products multiplying and existing in the environment of the plant in question, containing the listed microorganisms and their production.

The invention discloses a pretreatment method for enhancing anaerobic sludge digestion by virtue of a compound microorganism bacterium agent. The pretreatment method comprises the following steps: concentrating residual sludge, adding a compound microorganism bacterium agent, performing stirring reaction, inoculating the pretreated residual sludge into an anaerobic digestion pond according to a certain ratio, and performing mesophilic anaerobic digestion on anaerobic sludge, wherein the compound microorganism bacterium agent is prepared by mixed fermentation of 5%-20% of each of bacillus subtilis, streptomyces albus, streptomyces griseus, candida utilis, aspergillus sojae, lactobacillus bulgaricus, lactococcus lactis, rhodopseudomonas palustris, rhodopseudomonas capsulate and rhodopseudomonas spheroides, the total viable count is not less than 2*108 / ml, and the pH value of the compound microorganism bacterium agent is 3-4. According to the method, the sludge hydrolysis process can be accelerated, the sludge retention time can be shortened, the biological gas production rate can be increased, and the anaerobic digestion efficiency can be improved.

The invention belongs to the technical field of bio-fertilizer, particularly relates to bio-organic fertilizer prepared from soy protein wastewater sludge, and further discloses a preparation method of the bio-organic fertilizer. The preparation method of the bio-organic fertilizer includes: bacillus stearothermophilus, streptomyces albus, fine candida and staphylococcus epidermidis are used as a composite fermentation microbial agent to perform aerobic fermentation on the protein sludge so as to prepare the bio-organic fertilizer. The prepared bio-organic fertilizer is high in nutritive value and can be used as organic fertilizer.

A method for improving the fermentation level of salinomycin is to reduce its response to salinomycin biosynthesis by inactivating the type I PKS biosynthesis gene cluster located at SLNWT0868‑SLNWT0920 (1,173,724bp‑1,276,916bp) in Streptomyces albicans DSMZ41398. Body competition, to achieve the increase of salinomycin production. By adopting the method of the invention, the final fermentation yield of salinomycin can be increased by more than 8 times, and the laboratory shake flask level can reach 6.3g / L.

The invention provides a composite microbial preparation for garbage deodorization and a preparation method thereof. The composite microbial preparation is a reddish brown or brown liquid having fermentation-type sour and has the pH value within 3.4 to 4.0; and the total number of effective viable bacteria in the composite microbial preparation is greater than or equal to 1.0*10<9>CFU / mL, wherein the effective viable bacteria comprise 10<5>CFU / mL to 10<7>CFU / mL of saccharomyces cerevisiae, 10<6>CFU / mL to 10<8>CFU / mL of bacillus subtilis, 10<6>CFU / mL to 10<8>CFU / mL of bacillus coagulans, 10<6>CFU / mL to 10<8>CFU / mL of lactobacillus fermentium, 10<6>CFU / mL to 10<8>CFU / mL of lactobacillus buchneri, 10<5>CFU / mL to 10<7>CFU / mL of enterococcus faecium and 10<4>CFU / mL to 10<6>CFU / mL of streptomyces albus. The composite microbial preparation is complex in composition, stable in structure, nontoxic and harmless and cannot generate side effects. The composite microbial preparation can be used for effectively inhibiting the breeding and the growth of putrefying bacteria, pathogenic bacteria and other spoilage organisms and deodorizing garbage, excrement and sewage so as to reduce pollution and improve the environment.

The invention discloses streptomycete strains and a combined application thereof in prevention and treatment of pepper seedling blight, and relates to the technical field of microorganisms. The streptomycetes disclosed by the invention are separated and obtained from a deep soil sample of 10-15cm collected from the Hangzhou xixi wet land in Zhejiang by adopting a streptomycete separation technology, are identified by microbial taxonomy, are named streptomyces albus (streptomyces albus) 2013 and streptomyces fradiae (streptomyces fradiae) M931,S.albus2013; the streptomyces albus 2013 is preserved at the China Center for Type Culture Collection on June 19, 2013; the preservation number is CCTCC NO:M2013269; the S.fradiae M931 is preserved at the China Center for Type Culture Collection on December 25, 2013; and the preservation number is CCTCC NO:M2013712. The two bacterial strains can be fermented to generate active substances with relatively strong antagonistic effects on rhizoctonia solani kuhn (Rhizoctonia solani Kuhn), and can be used for preventing and treating plant diseases such as the pepper seedling blight; when fermentation products of the two bacterial strains are mixed to prepare the mixed preparation according to the mass percent of 3 to 2, the effect for preventing and treating the pepper seedling blight is more significant in comparison with the effect when two metabolites are respectively used as single preparations.

The invention discloses a pyrazomycin biosynthesis gene cluster, recombinant bacteria and an application of the recombinant bacteria, and particularly discloses cloning, sequencing, analysis and function research for the biosynthesis gene cluster of a natural product pyrazomycin which is produced from pure white streptomyces albus NRRL 3601 and used for tumor treatment as well as an application ofthe biosynthesis gene cluster. The whole gene cluster contains 24 genes. Biosynthesis of pyrazomycin can be blocked or increased through genetic manipulation for the biosynthesis genes. The genes andproteins of the genes can be applied to genetic engineering, protein expression and enzymic catalytic reaction of the compound, and can also be used for searching and discovering compounds, genes andproteins capable of being applied to medicine, industry or agriculture.

The invention relates to a streptomyces albicans X-18 and a method for producing ε-polylysine by using the bacteria, belonging to the technical field of bioengineering. The Streptomyces albicans X‑18 is isolated and screened from soil, and has been preserved with the preservation number CGMCC No.17536. Utilize described Streptomyces albicans X-18 to ferment and produce ε-polylysine, and by improving the fermentation medium and fermentation conditions, in combination with the method of intermediate feeding and adding inducers, the fermentation of ε-polylysine The output can reach 3.5‑4.2g / L. The invention mainly uses strains with independent intellectual property rights, and uses cheap cornstarch as the main component of the fermentation medium, thereby reducing the production cost and increasing the fermentation output.

The invention relates to a mutagenized strain of streptomyces albus TUST2, and a method for using the same to produce epsilon-polylysine and a salt of the epsilon-epsilon. The strain is a high-yield mutagenized strain of epsilon-polylysine selected and bred by means of ultraviolet mutagenesis, ultraviolet-chemical mutagenesis, N ion injection mutagenesis, etc from a strain of streptomyces albus TUST2 which is capable of producing epsilon-polylysine and is screened from the earth in Hainan province of China; the mutagenized strain is resistant to 10mg / ml or above S-(2 Aminoethyl )-L-Cysteine and can generate 10 to 30 g / L acid under optimal conditions; zymotic fluid is removed of the streptomyces albus by configuration and is filtered by diatomaceous earth to obtain clear filtrate and to weak acid type ion exchange resin to obtain coarse epsilon-polylysine products which is subjected to ultrafiltration and purification to get epsilon-polylysine and the salt of the epsilon-epsilon. The distribution of molecular weight of epsilon-polylysine is between 4000 and 6500Da.

The invention discloses a method for efficiently synthesizing mequinomycin A in streptomyces albus. The invention is based on the method of introducing site-directed mutant A penicillin binding protein gene into the heterologous expression host Streptomyces.albus of the mequinomycin A biosynthesis gene cluster to optimize the heterologous host, and the mequinomycin A acts on peptidoglycan transferase structural domain active sites of bacterial cell walls aPBP to effectively inhibit the synthesis of the cell walls of Gram-positive bacteria. Large-fragment assembly and promoter optimization arecarried out on the mequinomycin biosynthesis gene cluster by means of genetic engineering, and the mequinomycin A resistance gene MaPBP is introduced into the optimized strain II to further improve the mequinomycin resistance of the strain II. Finally, the heterologous expression yield of the mequinomycin A in the strain LX03 is increased by 110% compared with that of the original strain I. According to the invention, not only the genetic engineering modification method of large-segment gene clusters is described, but also a precedent that a Streptomyces. Albus host is optimized by a resistance gene to improve the yield of a target compound is started.

The invention relates to a fermented feed without antibiotics for animals and a preparation method of the feed. Grape residues are adopted as main fermentation raw materials and mixed with auxiliary components such as corn residues, cottonseed cakes and bean dregs, citric acid is also added, a compound bacterial liquid composed of lactic acid bacteria, yeast, bacillus subtilis and streptomyces albus which are mixed according to a specific proportion is adopted, and all the materials are fermented in a water content and pH range of a specific fermentation mixture to prepare the fermented feed without antibiotics. After the animals are fed with the feed, the intestinal microbial balance of the animals can be improved, the immunity is enhanced, the growth performance of the animals is improved, the prepared fermented feed can replace the role of antibiotics in feed, and the feed safety problem is effectively solved. The fermented feed which can replace feed antibiotics is prepared by means of the simple preparation method under the optimized fermentation conditions, the cost is low, and industrialization is easy.

The invention discloses engineering streptomycete for producing daunorubicin and a construction method of the engineering streptomycete. Streptomyces albus ZD11 is used as an original strain, and through in-vitro fishing of large fragments of a genome, engineering transformation of a pathway specific activation protein gene promoter, deletion of a synthetic branch and blocking of a competitive synthetic pathway, an engineering streptomycete strain ZD11-M4 capable of heterologously synthesizing the daunorubicin is constructed, and the shake-flask fermentation yield of the daunorubicin reaches 22.62 mg / g and is doubled compared with that of an original strain. According to the engineering streptomycete provided by the invention, heterologous expression of daunorubicin synthetic gene clustersis realized for the first time, and a technical foundation is laid for industrial production of the daunorubicin. Besides, an efficient expression method of a polyketone compound in a heterologous host provided by the invention has important research and application values for exploring novel polyketone antibiotics, reducing the fermentation cost and increasing the yield.

The invention discloses a microbial composition and a method for treating wastewater by using the same. The invention relates to a microbial composition for treating wastewater. The microbial composition comprises at least two of pseudomonas stutzeri, streptomyces rubrum, streptomyces albus, bacillus subtilis, bacillus mucilaginosus and halophilous actinomycetes. The wastewater meets one or two of the following conditions: a, the COD (Chemical Oxygen Demand) value reaches 90000-110000 mg / L; and b, the total nitrogen value reaches 3000 to 5000 mg / L. The method for culturing the biological composition by using the taurine industrial wastewater, provided by the invention, is low in cost and good in effect; the waste liquid in the taurine production is used for culturing the microbial composition, so that COD and total nitrogen in the waste water can be reduced (more than 95% of COD can be degraded at most); and the wastewater can be used for culturing a microbial composition, and bacteria obtained by fermentation can be used as a biological fertilizer and have a fertility increasing effect on vegetables and green plants.

The invention provides a streptomyces albus decomposition accelerator and the application thereof in saline-alkali soil straw decay soil condition improvement. The decomposition accelerator comprises 2.2-3.1 parts of compound microorganism bacterial powder, 36-42 parts of Chinese elm barks, 24-29 parts of rose pollen, and 6-11 parts of scenedesmusarcuatus. The compound microorganism bacterial powder comprises 44*109 CFU / g streptomyces albus, 30*109 CFU / g rhodopseudomonas capsulata, 5*109 CFU / g lactobacillus bulgaricus, and 14*109 CFU / g bacillus subtilis. After the decomposition accelerator is adopted in saline-alkali soil straw decay soil condition improvement for a month, the content of salt in a 0-30 cm soil layer is 0.28-0.31%, the pH value of soil is 7.3-7.6, the mean size of the soil granular structure is 3.0-3.5 mm, and the total porosity of soil is 54-59%.

The invention relates to a strain of streptomyces albus for preventing and controlling aspergillus flavus and aflatoxin and application thereof. Microbes are separated from intestinal tracts of sea cucumbers under an oligotrophic culture condition by using a culture medium only containing agar powder; and in addition, the strain of streptomyces albus is separated from the intestinal tracts of healthy sea cucumbers. After being fermented, the streptomyces albus generates a great number of active secondary metabolites capable of inhibiting the growth of a pathogenic fungus of the aspergillus flavus and the synthesis of the aflatoxin. In addition, the streptomyces albus can completely inhibit the pollution of fungi and fungal toxin in the storage process of grains such as peanuts; and wide application prospects are realized in the field of grain safety.

The invention belongs to the field of biochemical engineering, and relates to an extraction method of polylysine. The method comprises the following steps: 1) centrifuging fermentation liquor of streptomyces albus for producing polylysine, and removing mycelia to obtain supernate; (2) adjusting the pH value of the obtained supernate, continuously centrifuging, and removing protein floccules to obtain clear fermentation liquor; (3) carrying out ion exchange on clarified fermentation liquor obtained by pulping through weakly acidic cation exchange resin D152, and filtering; 4) desorbing the filtered resin to obtain an ion exchange liquid; 5) concentrating the ion exchange liquid, and decolorizing with activated carbon; performing suction filtration with filter paper to remove activated carbon, precipitating filtrate with ethanol, and dissolving and precipitating with a small amount of water; and 6) after the organic solvent is completely volatilized, feeding residues for freeze drying to obtain powder, namely the polylysine. The method is simple and easy to implement, and the purity and the recovery rate of polylysine can be improved.

The invention discloses a genetically engineered bacterium of high-yield epsilon-polylysine, namely streptomyces albus pd9-pld3, and the preservation number of the genetically engineered bacterium is CCTCC (China Center for Type Culture Collection) M 20211549. The invention also discloses an application of the engineering bacterium in fermentation production of epsilon-polylysine, and an optimized fermentation culture medium for the streptomyces albus pd9-pld3, and also discloses an application of the engineering bacterium in fermentation production of epsilon-polylysine, and an optimized fermentation culture medium for the streptomyces albus pd9-pld3. The yield of the epsilon-polylysine of the streptomyces albus disclosed by the invention is obviously improved compared with that of an original strain, and the inhibition on an epsilon-polylysine degrading enzyme can be realized.

Disclosed is a high-throughput transcriptional assay format in Actinomycete bacteria, and Streptomyces spp. in particular, that leverages eGFP, inserted both at a neutral site and inside the biosynthetic cluster of interest, as a read-out for secondary metabolite synthesis. Using this approach, a silent gene cluster in Streptomyces albus J1074 was induced. The cytotoxins etoposide and ivermectin were revealed as potent inducers, allowing the isolation and structural characterization of nearly 20 novel small molecule products of the chosen cluster. One of these molecules is a novel antifungal, while several others inhibit a cysteine protease implicated in cancer. Studies addressing the mechanism of induction by the two elicitors led to the identification of a pathway-specific transcriptional repressor that silences the gene cluster under normal growth conditions. The successful implementation of this approach will allow future discovery of cryptic metabolites with useful bioactivities from Actinomycete bacteria.