Application of salinomycin in preparing medicament for resisting various human malignant tumors

A technology for malignant tumors and salinomycin, applied in the field of chemical medicine, can solve the problem of unclear influence of stem cells on inhibition

Active Publication Date: 2011-09-21
王毅 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although salinomycin can kill breast cancer stem cells in mice, inhibit new breast cancer cells and slow down their growth rate, but the inhibitory effect on other human tumors and stem cells is still unclear. conduct in-depth research

Method used

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  • Application of salinomycin in preparing medicament for resisting various human malignant tumors
  • Application of salinomycin in preparing medicament for resisting various human malignant tumors
  • Application of salinomycin in preparing medicament for resisting various human malignant tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Embodiment 1: In vitro anti-tumor cell proliferation experiment of salinomycin

[0011] The selected cell lines include human lung cancer cell A549, skin cancer cell A-431, pancreatic cancer cell BxBCO, colon cancer cell Caco2, colorectal cancer cell HT-29, liver cancer cell HepG2, tongue cancer cell SCC-9, renal cell carcinoma Cell 786-O, brain metastatic prostate cancer cell DU-145, etc. Take tumor cells in the logarithmic growth phase to make a certain concentration of cell suspension and inoculate them in a 96-well plate. After the cells are cultured for 48 hours, take out the culture plate, add 50ul of pre-cooled 50% trichloroacetic acid to each well, and let it stand After 5 minutes, move it into a 4°C refrigerator and let it stand for 1 hour, take it out and wash it 5 times with deionized water, and dry it in the air. After it is completely dry, add 100ul of 0.4% SRB prepared with 1% acetic acid to each well, and pour it out after 20 minutes of staining Dye solu...

Embodiment 2

[0021] Example 2: Effect of Salinomycin on Human Lung Cancer Cell Line A549 Stem Cells

[0022] 1) Isolation of lung cancer stem cells from human lung cancer cell line A549:

[0023] A549 cell line was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). These cells were cultured in medium recommended by ATCC and tested in logarithmic growth phase. The ALDEFLUOR kit (StemCell Technologies, Durham, NC, USA) was used to react with human ALDH1A1 as much as possible to determine cancer stem cells. In simple terms, cells expressing fluorescent marker ALDH1A1 were detected and sorted by flow cytometry ( BD Biosciences, San Jose, CA, USA). Specifically, ALDH1A1 activation is based on the expression of the ALDEFLUOR inhibitor diethylaminobenzaldehyde (DEAB). Data were analyzed using Quest software (BD Biosciences).

[0024] [Flow cytometry detection and sorting of cells positive for the expression of the fluorescent marker ALDH1A1 are shown in Figure 2-1] ...

Embodiment 3

[0027] Example 3: The animal experiment on the effect of salinomycin on human lung cancer A549 stem cells is in the process of experimentation.

[0028] Nod-scid immune triple-deficient mice were purchased from Shanghai Slack Experimental Animal Co., Ltd. 5000 ALDH-positive A549 cells were suspended in 1 ml of Matrigel medium (Viglass Biotechnology (Beijing) Co., Ltd.), diluted 1:2 in DMEM, and injected subcutaneously into NOD-SCID mice. Tumor formation was detected by palpation. Salinomycin injection was given 24 hours later. The use of animals was carried out in accordance with the relevant national regulations. Salinomycin (5 mg / kg / day) or paclitaxel (5 mg / kg / day) was injected intraperitoneally once a day to 6 mice in each group for a total of four weeks.

[0029] In summary, salinomycin has the potential and prospect of being developed into an antitumor drug.

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Abstract

The invention discloses an application of salinomycin in the preparation of medicaments for resisting various human malignant tumors. Salinomycin adopted by the present invention assists in inhibiting the growth of a plurality of human tumor cells and metastatic tumor cells. Further, salinomycin is capable of killing a plurality of tumour stem cells such as lung cancer stem cells. Salinomycin haspotentials to be adopted in the exploitation of anticancer medicaments aimed at tumour stem cells, and has important values in the exploitation of medicaments for resisting various human malignant tumors such as lung cancers, skin cancers, pancreas cancers, colon cancers, liver cancers, rectal cancers, brain cancers, renal cell carcinoma, brain-metastatic prostate cancers, and the like.

Description

1. Technical field [0001] The invention relates to the field of chemical medicine. It specifically relates to the application of a salinomycin in the preparation of anti-human malignant tumor drugs. 2. Background technology [0002] In the past 30 years, the morbidity and mortality of malignant tumors in the world have shown an obvious upward trend. The mortality rate of malignant tumors in our country is increasing by 1.8% every year, and the incidence rate is increasing by 2.5% every year. At the same time, the ranking of high-incidence malignant tumors in China has also undergone significant changes. The order of major malignant tumors in the 1970s and 1980s was: gastric cancer, esophageal cancer, liver cancer, lung cancer, and cervical cancer. It has evolved into: lung cancer (breast cancer for women), liver cancer, stomach cancer, esophagus cancer, colon / rectal cancer or prostate cancer. Because its pathogenesis has not yet been ascertained, the current treatment me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/351A61K9/20A61K9/48A61K9/14A61K9/08A61K9/02A61P35/00
Inventor 王毅刘亚光廖端芳
Owner 王毅
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