Purification method of salinomycin

Abstract

The invention relates to a purification method of salinomycin. The purification method of salinomycin disclosed by the invention comprises the following steps: obtaining a salinomycin filter cake directly from salinomycin fermentation broth through a flocculation process, saving a saponification process, thereby improving the yield and purity of salinomycin. In the implementation process, preferably, flocculants provided by the invention are used, the organic flocculant and the inorganic flocculant are used in a mixed state and the ratio of the organic flocculant and the inorganic flocculant is adjusted to improve the yield of salinomycin. In the invention, after a filter aid is added, and a mixed solution is subjected to ultrasonic treatment, so that salinomycin can be better precipitated, and then concentrated and crystallized in the subsequent steps to greatly improve the purity of salinomycin. The energy is saved and the cost is lowered in the actual production process to protect the environment.

Description

technical field [0001] The invention relates to a purification process of salinomycin, in particular to a purification process of salinomycin after fermentation, and belongs to the technical field of antibiotic production. Background technique [0002] Salinomycin (salinomycin) belongs to polyether monocarboxylic acid, which is produced by fermentation of Streptomyces albus. It has a special ring structure and is a typical ionophore antibiotic. The affinity of rubidium ions is particularly strong, which enhances the permeability of cations necessary for biology through the lipid barrier on the membrane, hinders the transmission of cations inside and outside the cell, changes the concentration of ions inside and outside the cell, affects the osmotic pressure, and finally causes the cell to disintegrate , Play a bactericidal effect. Salinomycin is highly sensitive to Gram-positive bacteria, fungi, viruses and malaria parasites. The effect of preventing coccidiosis is 100%, an...

AI Technical Summary

Problems solved by technology

Although the yield of salinomycin prepared by this method is improved to a certain extent, the quality is poor and the purity is low.

[0007] Another example is the Chinese Patent Publication CN1078263A which discloses the preparation method of salinomycin and its sodium salt crystalline product. The preparation method is to add filter aid to filter the fermented liquid after acidification, and the filter residue is extracted with alcohol, filtered, and the filtrate is decolorized and filtered with activated carbon. 1. Take the filtrate to concentrate, and add water to get the product. The preparation method of the invention is simple in process and low in cost, but the yield of the prepared salinomycin is only about 70%, and the low yield cannot meet the market demand for salinomycin

[0008] Another example is Chinese patent application 201510026181X, which discloses a process for extracting salinomycin. The solution adopted in the present invention is to add a certain amount of flocculant to the fermentation broth, and then perform plate and frame filtration, and directly squeeze and granulate the filter cake. , drying, and mixing auxiliary materials to obtain the finished product of salinomycin medicine, although the present invention can shorten the reaction time in the production process, improve production efficiency, reduce energy consumption, and reduce costs, the yield and purity of the salinomycin produced by the invention are both low. relatively low

[0009] In summary, the current extraction and purification methods for salinomycin are either costly when used in actual industrial production, or the yield and purity cannot meet the demand. Therefore, the purity and yield of salinomycin can be improved while simplifying the production steps. It is very necessary to reduce the production cost and improve the production quality