Purification method of salinomycin
A purification method, the technology of salinomycin, which is applied in the direction of organic chemistry, etc., can solve the problems of low yield that cannot meet the demand of salinomycin, yield and purity that cannot meet the demand, low yield and purity of salinomycin, etc., and achieve Eliminate the effect of saponification process, yield and purity improvement, yield and purity improvement
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Embodiment 1
[0050] A kind of purification method of salinomycin of embodiment 1
[0051] The specific steps are:
[0052] (1) Acidify 100 liters of salinomycin fermentation broth with hydrochloric acid to adjust the pH to 4.5, then add 100 g of aluminum potassium sulfate to the acidified fermentation broth, and incubate at 20° C. for 15 minutes to obtain a flocculated mixture A for subsequent use;
[0053] (2) Add 200 g of diatomaceous earth to the mixture A obtained in step (1) and stir evenly, then perform ultrasonic treatment at 2°C, the ultrasonic intensity is 30KHz, and the time is 15min to obtain the mixture B, which is transferred to the plate and frame press Filter in the filter, discard the filtrate, obtain filter cake C, and set aside;
[0054] (3) Flash-dry the filter cake C obtained in step (2) until the water content is 3%, to obtain bacterial powder D, which is set aside;
[0055] (4) The bacteria powder D obtained in the step (3) is leached twice with 3 times of 95% ethan...
Embodiment 2
[0060] A kind of purification method of salinomycin of embodiment 2
[0061] The specific steps are:
[0062] (1) Acidify 100 liters of salinomycin fermented liquid with acetic acid to adjust the pH to 5, then add 800 g of chitosan to the acidified fermented liquid, incubate at 25° C. for 20 min to obtain flocculated mixture A, and set aside;
[0063] (2) Add 400g of perlite to the mixture A obtained in step (1) and stir evenly, then perform ultrasonic treatment at 2°C, the ultrasonic intensity is 35KHz, and the time is 16min to obtain mixture B, which is transferred to plate and frame filter press Filter in the machine, discard the filtrate, obtain filter cake C, and set aside;
[0064] (3) Flash-dry the filter cake C obtained in step (2) until the moisture is 4%, to obtain bacterial powder D, which is set aside;
[0065] (4) The bacterial powder D obtained in the step (3) is leached twice with 3 times of 95% ethanol solution to obtain the extract E, which is set aside;
...
Embodiment 3
[0070] A kind of purification method of salinomycin of embodiment 3
[0071] The specific steps are:
[0072] (1) Acidify 100 liters of salinomycin fermentation broth with phosphoric acid to adjust the pH to 5.5, then add 300 g of seaweed gel to the acidified fermentation broth, and heat it at 30° C. for 25 minutes to obtain a flocculated mixture A for later use;
[0073] (2) Add 400g of cellulose to the mixture A obtained in step (1) and stir evenly, then perform ultrasonic treatment at 2°C, the ultrasonic intensity is 36KHz, and the ultrasonic time is 18min to obtain the mixture B, which is transferred to the plate and frame press Filter in the filter, discard the filtrate, obtain filter cake C, and set aside;
[0074] (3) Flash-dry the filter cake C obtained in step (2) until the water content is 5%, to obtain bacterial powder D, which is set aside;
[0075] (4) The bacterial powder D obtained in the step (3) is leached twice with 3 times of 95% ethanol solution to obtain...
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