9 results about "Direct assay" patented technology

The invention discloses an ultratrace mercury isotope analysis method, and relates to the technical field of environmental analysis. The method comprises the following steps: purging a liquid sample to be detected by nitrogen in a stannous chloride atmosphere, and trapping and concentrating mercury into a gold trapping tube; pyrolyzing the pyrolytic gold trapping tube to release mercury vapor; pyrolytic mercury vapor and thallium isotope internal standard aerosol are mixed to serve as a sample injection sample, and the sample injection sample is introduced into a multi-receiver inductively coupled plasma mass spectrometry; acquiring a mercury instantaneous signal and a thallium isotope signal; and calculating the mercury isotope ratio by adopting least square linear regression fitting in combination with a thallium internal standard ratio correction algorithm. According to the method, the instantaneous mercury isotope signal is generated by online combination of gold trapping and multi-receiver inductively coupled plasma mass spectrometry, the instantaneous signal is strategically collected and processed, the determination concentration of isotope analysis can be greatly reduced, the sensitivity of the method is remarkably improved, and direct determination of mercury isotope composition in a water sample with the concentration of 10 ng L <-1 > is met.

The present disclosure relates to a method and an extrusion line in which an additive content, in particular, a carbon black content, can be determined inline during extrusion of an extrusion product. For this purpose, an initial measurement is carried out in which a layer thickness and a refractive index of the extrusion product are determined, whereupon a continuous in-line measurement of the current additive content is carried out by a stationary radar measuring device. Thus, the layer thickness is set and the additive content is determined directly by the radar measuring device and controlled by setting the additive feed rate, in particular as a close-loop control.

The present application relates to an assay method using affinity crosslinking of DNA-linked ligands to proteins to enable small molecule screening and determination of apparent affinity constants of ligands to the proteins. This method has been applied to determine 96 compounds' dissociation constants to a protein target simultaneously, and directly determine a compound's IC50 against five protein targets concurrently in crude cell lysates. Additionally, this approach was used to screen a Library of Pharmacologically Active Compounds (LOPAC) library against dihydrofolate reductase (eDHFR), enabling the discovery of a novel eDHFR inhibitor (IC50=7.9 μM). An assay kit and the method of uses are within the scope of this disclosure.

The invention belongs to the field of chemistry, and relates to a method and system for directly measuring the content of titanium carbide in carbide slag through X-ray diffraction. The method comprises the following steps: preparing a series of carbide slag standard samples containing different known TiC contents; adopting an X-ray diffraction analyzer to respectively scan the standard sample and the to-be-detected sample under the same parameter condition to obtain an XRD (X-Ray Diffraction) spectrum; selecting a characteristic diffraction peak of TiC at 2theta of 41.9 + / -0.2 degrees, measuring the intensity of the characteristic diffraction peak, and drawing a standard working curve by taking the TiC mass fraction of the standard sample as an abscissa and the corresponding peak intensity as an ordinate; and measuring the characteristic diffraction peak intensity of a sample to be measured, substituting the characteristic diffraction peak intensity into the standard working curve, and calculating to obtain the mass fraction of TiC in the sample to be measured. The method has the characteristics of rapidness and accuracy, the analysis time of TiC in the carbide slag is shortened from 2 hours to 15 minutes, the operation conditions are improved, the labor intensity is reduced, and the analysis process is greatly shortened.

The invention discloses an experimental method capable of directly measuring the anisotropy coefficient in any direction in a panel. The experimental method comprises the following steps: S1, determining the shape and size of an experimental sample; s2, cutting the experimental sample in the step S1 from a to-be-tested plate; s3, a flanging die and a rolling die are prepared; s4, performing flanging forming on the experimental sample by adopting a flanging die, and forming a straight wall area around the hole of the experimental sample; s5, placing the experimental sample on a calendering mold for calendering forming, and enabling the straight wall area to generate deformation required for measuring the anisotropy coefficient of the plate in any direction; s6, characteristic parameters of the straight wall area are obtained; and S7, determining the anisotropy coefficient in each direction in the experimental sample by using the characteristic parameters. According to the invention, the plate can be simultaneously and continuously in a uniaxial tensile stress state in all directions during deformation, and the obtained test data is ensured to be in a continuous form under the same experiment condition, so that the anisotropy coefficient in any direction in the plate surface can be accurately measured.

The invention discloses an air pressure measurement-based carbon sequestration amount indirect measurement method, which is implemented on a closed wet-process carbon sequestration system equipped with an inlet gas flowmeter and a reaction kettle pressure gauge, and CO2 with the total input amount of Nin in the system can be divided into three parts, namely carbon sequestration amount NI, water soluble amount NII and free amount NIII according to the distribution position and the occurrence state. Nin and NIII are directly measured through an inlet gas flowmeter and a reaction kettle pressure gauge. Meanwhile, the method comprises a first round of pressure measurement, that is, a corresponding relation of NII in an effective stable pressure Pd range under the liquid-gas two-phase condition is calibrated and established in advance. And then carrying out a second round of pressure measurement in a solid-liquid-gas three-phase carbon sequestration process, and obtaining NI according to a formula NI = Nin-NII-NIII by combining the pre-measured NII with the directly measured Nin and NIII. According to the method, sampling is not needed, errors caused by non-uniform materials and non-representative sampling are avoided, the carbon sequestration amount is deduced by directly measuring the pressure, the overall carbon sequestration level of the materials can be reflected, and the analysis accuracy of the carbon sequestration amount is remarkably improved.

The application discloses a method for ultra-trace mercury isotope analysis and relates to the technical field of environmental analysis. The method comprises the following steps: blowing nitrogen to the liquid sample to be measured under the atmosphere of stannous chloride, and concentrating mercury into a gold trapping tube; pyrolyzing the gold trapping tube to release mercury vapor; mixing the pyrolyzed mercury vapor with thallium isotope internal standard aerosol, and then introducing the mixture into a multi-receiver inductively coupled plasma mass spectrometer as a sample; collecting mercury transient signals and thallium isotope signals; and calculating the mercury isotope ratio by using a least square linear regression fitting combined with a thallium internal standard ratio correction algorithm. The application generates transient mercury isotope signals by using the online combination of gold trapping and the multi-receiver inductively coupled plasma mass spectrometer, strategically collects and processes the transient signals, significantly improves the sensitivity of the isotope analysis by greatly reducing the determination concentration, and meets the direct determination of mercury isotope composition in 10 ng / L ‑1 grade concentration water samples.

The application discloses a kind of indirect determination methods of carbon fixation amount based on air pressure determination, is implemented in a closed wet carbon fixation system equipped with inlet gas flow meter and reactor pressure gauge, total input in system is N in CO2According to distribution position and occurrence state, can be divided into carbon fixation amount N I , water-soluble amount N II , free amount N III Three parts.By inlet gas flow meter and reactor pressure gauge directly determine N in And N III , simultaneously, this method contains first round pressure determination, i.e.prior calibration and establish the corresponding relationship of N II In the range of effective stable pressure P d Under liquid-gas two-phase condition.N II Then carry out the second round pressure determination of solid-liquid-gas three-phase carbon fixation process, combine the directly determined N in , N III , according to formula N I =N in -N II -N III Obtain N I The application does not need sampling, avoids the error caused by material uneven, sampling not representative, derives carbon fixation amount by direct measurement of pressure, can reflect the overall carbon fixation level of material, significantly improves the accuracy of carbon fixation amount analysis.

The invention belongs to the technical field of gold content analysis in a geological exploration ore dressing and smelting process, and particularly relates to a method for directly measuring gold in ore by ICP-MS (Inductively Coupled Plasma Mass Spectrometry) applying an internal standard ion matrix strength agent. After a (1 + 1) aqua regia hot plate is used for heating an acid-soluble sample, complex operations such as separation and enrichment are not needed, a to-be-detected solution is obtained through direct suction filtration, then the inductively coupled plasma mass spectrometry is directly adopted for determination, meanwhile, an internal standard ion matrix strength agent is added, matrix adjustment is conducted, and complex matrix interference of the sample is reduced. According to the method, separation and enrichment are not needed in the pretreatment stage, an inductively coupled plasma mass spectrometer is matched for determination, and an internal standard ion matrix strength agent is added, so that the method is simple to operate, high in recovery rate, high in detection sensitivity, accurate and reliable in result and high in stability, and an excessive dilution multiple is not needed; the technical problems existing in the existing gold content analysis technology are solved.