57 results about "Kitasamycin" patented technology

The invention provides kitasamycin microcapsule and a preparation method and application thereof, which pertains to a feed drug additive field, in particular relates to novel kitasamycin preparation and a preparation technique thereof, and the application thereof in the preparation of livestock and poultry antibiotic growth promoter. The kitasamycin microcapsule of the invention comprises the kitasamycin and medical auxiliary materials; the weight of the medical auxiliary materials is 2-16 times of that of the kitasamycin material; the microcapsule is prepared through mixing the kitasamycin with heated and melted medical auxiliary materials and spray cooling. The kitasamycin microcapsule eliminates the bitterness of kitasamycin, intensifies the delayed release function of kitasamycin, reduces the stimulation of the kitasamycin to the stomach and the damage and decomposition degree thereof in the stomach, and enhances the effective intestines concentration and the functioning time of kitasamycin; the method for preparing the kitasamycin microcapsule is a wholly close manufacturing process and a wholly close material conveying system, thus decreasing flying of dust and material loss with has high yield of the products.

The invention discloses a monoclonal antibody for kitasamycin residual detection and a preparation method and application thereof. The monoclonal antibody is secreted by a hybridoma KA / 2A9, which is collected by CCTCC (China Center for Type Culture Collection) and has the collection number of C201184. The preparation method comprises the steps of: (A) coupling hapten kitasamycin with bovine serum albumin so as to obtain an immunogen; (B) coupling hapten kitasamycin with ovalbumin so as to obtain a coating antigen; (C) preparing the monoclonal antibody secreted by the hybridoma KA / 2A9 by using the immunogen; (D) coating a solid phase carrier by using the coating antigen; (E) extracting a sample to be detected by using metaphosphoric acid in the presence of ethanol, carrying out back extraction by using ethyl acetate, blow-drying by using nitrogen gas, and diluting the sample so as to obtain a substance to be detected; and (F) carrying out enzyme-linked immunoassay on the substance to be detected. A kit for kitasamycin residual detection comprises an ELISA (Enzyme-Linked Immunosorbent Assay) plate coated by the coating antigen kitasamycin-AOOA-OVA, a kitasamycin standard solution, a goat anti-mouse IgG (Immunoglobulin G) antibody working solution labeled by a horseradish peroxidase, a concentrated phosphate buffer, a concentrated washing solution, a substrate mixture and a stop solution. The method has the characteristics of simplicity, convenience, rapidness, sensitiveness and accuracy.

The invention relates to a Kitasamycin fermenting culture medium and belongs to the field of production of kitasamycin. The fermenting culture medium comprises the components in percentages by weight: 1.5-4.5% of starch, 0.1-0.3% of glucose, 2.0-4.0% of soybean cake powder, 0.5-1.5% of fibroin powder, 0.2-0.5% of calcium carbonate, 0.006-0.015% of amylase, 2.0-3.5% of soya-bean oil, 0.01-0.06% of GPE, 0.05-0.1% of potassium dihydrogen phosphate, 0.15-0.55% of ammonium sulfate, 0.04-0.06% of manganese chloride, 0.005-0.007% of zinc sulfate as well as an emulsifier which is tween 80. The tween 80 is uniformly mixed with the soy-bean oil and is then mixed with other comments; the pH of the fermenting culture medium is 6.3-7.5. By using the culture medium, the fermenting level of kitasamycin can be improved. Moreover, after fermentation of the culture medium, oil in fermenting liquor is fully used, so that the use level of the soy-bean oil is reduced, and the production cost is lowered; generation of emulsification in the extraction process is further reduced to facilitate extraction of a product in a purifying section, so that the extraction yield is improved, and the product quality is improved to a great extent.

The invention relates to a Kitasamycin monoclonal antibody hybridoma cell strain SML and an application thereof, and belongs to the field of food safety immune detection. The hybridoma cell line SML is deposited in the general microbiology center of the Council for the preservation and administration of microbial strains in China, with the preservation number of CGMCC No. 17392. The complete antigen of kitasamycin and equivalent freund's adjuvant are mixed and emulsified to immunize BALB / c rats. Complete freund's adjuvant is used for the first immunization, incomplete freund's adjuvant is used for several times to strengthen immunization, and finally, kitasamycin complete antigen is used for sprint immunization. Splenic cells of rats with high titer and low IC50 are fused with myeloma cells of rats by a PEG method, cultured and screened; then the cells are screened by indirect competitive enzyme-linked immunosorbent assay and subcloned three times to finally obtain a monoclonal antibody hybridoma cell strain. The monoclonal antibody secreted by the cell strain has good specificity and detection sensitivity to kitasamycin, can realize the detection of kitasamycin residues in aquatic products, and has practical application value.

The invention discloses a production method of enteric-coated kitasamycin for feed, which comprises the following process steps: step one, the preparation of drug-loaded pellets; step two, inner layer sustained-release coating of the drug-loaded pellets, which prolongs the release and acting time of kitasamycin; and steps three, outer layer enteric coating of the drug-loaded pellets, which ensures the release in succus entericus and small or no release in gastric juice. The method has the advantages that the kitasamycin is subjected to pellets pelletizing and 99 percent of the prepared granulums can pass through 24 meshes, so the dust is greatly reduced and the fluidity is increased; the coating of the inner layer sustained-release agent (HPMC) prolongs the release and acting time of the kitasamycin, reduces medication times and reduces the medication cost; and a layer of enteric substance, namely acrylic resin-III is sprayed and coated on the outer layer of particles. The substance protects the kitasamycin which is a weakly alkaline antibiotic from being damaged by gastroc acid in stomach, quickly disintegrates after entering enteric canal and releases the kitasamycin; and then the kitasamycin is absorbed by gastrointestinal mucosa into blood drug to play a role of restraining the reproduction of pathogenic microorganism and preventing diarrhea. Insoluble in the stomach, kitasamycin coating formulations have no pessimal stimulation on the stomach, and cannot result in regurgitation and vomiting. In addition, the sustained-release formulation, namely the kitasamycin prolongs the acting time so the medication times is reduced, the medication cost of farmers is reduced, and the economic benefit is improved.